• Title/Summary/Keyword: Germination Percentage

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Effects of Media and Plant Growth Regulators on Germination of Somatic Embryos of Aralia elata Seem (두릅나무 체세포배(體細胞胚)의 발아(發芽)에 미치는 배지(培地) 및 식물생장조절제(植物生長調節劑)의 영향(影響))

  • Park, Cheol-Ho;Lee, Youn-Su;Jhang, Han-Ho;Kim, Nam-Soo;Shin, Young-Boum
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.3
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    • pp.241-245
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    • 1994
  • Effects of media and plant growth regulators on the germination of somatic embryos of Angelica tree(Aralia elata Seem.) was studied for the mass production of Angelica tree through tissue culture. MS medium was found to be the most effective for the germination of somatic embryos(65% germination rate), Among the MS medium, the medium containing 25% less inorganic salts and 1% less sucrose was found to be the most effective. Gelling agent with 0.2-0.3% gelrite promoted the germination of somatic embryo$(65{\sim}70%)$ and caused good growth of shoots and roots. 0.1 mg/l of BA and kinetin treatment caused $65{\sim}70%$ germination rate of somatic embryos and good growth of shoots and roots, and resulted in high percentage of dry matter. 1mg/l or 5 mg/l treatment of putrescine, and 10 mg/l treatment of spermidine caused 90% germination rate of somatic embryos and good growth of plant organs, and inhibited vitrification of regenerated plants.

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Effects of Several Chemicals and Burial of Seeds into the Soil on Dormancy-breaking of Weed Species (잡초(雜草) 종자(種子)의 휴면타파(休眠打破)에 대한 화학물질(化學物質)과 토양(土壤) 내(內) 종자매몰(種子埋沒)의 효과(效果))

  • Shim, S.I.;Lee, S.G.;Kang, B.H.
    • Korean Journal of Weed Science
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    • v.18 no.4
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    • pp.295-303
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    • 1998
  • The seed dormancy is one of the peculiar characteristics of a number of weed species and it makes difficulties in weed control. To clarify the mechanism of seed dormancy, several chemicals such as $KNO_3$, KOH, thiourea, and $H_2O_2$ and phytohormone($GA_3$) were treated to dormant seeds. Among the species treated with several chemicals, the germination percentages of Setaria glauca, Ambrosia trifida and Ranunculus sceleratus were increased with $KNO_3$ and those of S. glauca, R sceleratus were increased with thiourea. Hydrogen peroxide promoted the germination of Setaria viridis and S. glauca. Germination percentages of S. viridis, S. glauca and Cyperus saraguinolentus were increased with enzyme treatment using pectinase. GA treatment enhanced the geim.ination of Eleusine indica and R sceleratus but the other species were affected slightly. Especially. E. indica showed linearity in the relationship between germination percentage and GA concentration. So, It seemed that E. indica can be used as a bioassy material for GA. Considering the phenological habits of weed species, the seeds were buried under soil for long time(more than 1 month) over winter. When seeds were buried in soil, the degree of dormancy was drastically decreased. Especially, germination of seeds buried were increased under alternating temperature. The germination rates of Persicaria ssp. and Chenopodium ssp. were increased by 50% order alternating temperature after burial for seven weeks.

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Germination Behaviors and Seed Longevities of Three Ulmus Species in Korea (한국산(韓國産) 느릅나무속 3수종(樹種)의 종자저장성(種子貯藏性) 및 발아특성(發芽特性))

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.438-444
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    • 2011
  • This study was conducted to investigate temperature effect on seed germination behaviors and seed viability changes by drying periods of three Ulmus species (Ulmus davidiana var. japonica (Rehder) Nakai, U. pumila L., and U. parvifolia Jacq.) distributed in Korea. Statistical analysis showed that temperature had a significant effect on mean germination time in three Ulmus species, but its effect on germination percentage was only shown in U. parvifolia (p<0.01). U. davidiana var. japonica and U. pumila germinated relatively consistent in all temperature conditions, especially the latter showed high germination behaviors in low temperatures (10-15$^{\circ}C$). U. parvifolia germinated well in high temperature (25$^{\circ}C$), while this species rarely germinated in low temperature conditions (10-20$^{\circ}C$). The results confirm that seeds of U. pumila and U. parvifolia are orthodox seeds which can be stored under 4-8% moisture condition, and U. davidiana var. japonica seed is recalcitrant seed which is difficult to store for a long time. In conclusion, these differences of the germination behaviors for three Ulmus species seem to be adaptive regimes of their natural habitats, respectively.

Performance of Acacia senegal (L.) Wild Seedlings Growth under some Tree Manures and NPK Fertilizers in Nursery Site

  • Daldoum, Daldoum Mohamed A.;Hammad, Ghassan Habib
    • Journal of Forest and Environmental Science
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    • v.31 no.4
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    • pp.303-311
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    • 2015
  • This study aimed to assess the effects of tree manures in comparison with NPK fertilizer on growth of Acacia senegal seedlings. It was conducted in the nursery of the Faculty of Forestry, University of Khartoum, Shambat (Lat.: $15^{\circ}$ 39' 387" N and Long.: $32^{\circ}$ 30' 871" E), during August 2008 and January 2009. The growing media were prepared by mixing ground foliage of trees with sandy soil (weight/volume) as follows, Albizia lebbeck (AL): 25 g, 50 g and 75 g; Azadirachta indica (AZ): 25 g, 50 g and 75 g; Khaya senegalensis (KH): 25 g, 50 g and 75 g; NPK fertilizer: 30 g per seedling. Albizia lebbeck and Azadirachta indica manures and NPK fertilizer have stimulated the Acacia senegal seed germination percentage in comparison with the control, with respective values of 89, 82.7, 81 and 71%. Khaya senegalensis manure has suppressed the Acacia senegal seed germination percentage with a value of only 49%. Effects of treatments on the seedlings growth parameters varied in the following percentages, in comparison to the control, shoot height: AZ 62.9%, AL 46%, KH 9.9% and NPK 27.8%; root length: AZ 25.8%, AL 31.5%, KH 30.6% and NPK 4.4%; diameter: AZ 75.2%, AL 37.1%, KH 34.3% and NPK 20%; Shoot biomass: AZ 319%, AL 195.2%, KH 57.1% and NPK 42.9%; root biomass: AZ 288.9%, AL 116.7%, KH 55.6% and NPK 16.7%%;. Thus, the effectiveness of the used substrates on the growth performance of Acacia senegal seedlings occurred in the following descending order: Azadirachta indica> Albizia lebbeck>Khaya senegalensis>NPK fertilizer. These results assert clearly the ameliorative and fertilizing characteristics of tree manures that can be reliably used for raising seedlings stocks in the nurseries.

Effect of Basal Medium and Plant Growth Regulator on in vitro Plant Regeneration from Axillary Buds of Walnut New Cultiver "Sinlyeong"

  • Kwon, Young Hee;Lee, Joung Kwan;Kim, Hee Kyu;Kim, Kyung Ok;Park, Jae Seong;Huh, Yoon Sun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.15-15
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    • 2019
  • The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

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Effect of Seed Moisture Contents and Cylinder Speed of Thresher on the Mechanical Damage and Germination of Soybean Seeds (콩의 기계 탈곡시 종실수분 함량과 급동속도에 관한 연구)

  • Moon, Yun-Ho;Hwang, Young-Hyun;Lee, Young-Ho;Kim, Seok-Dong;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.150-155
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    • 1986
  • An experimental study was undertaken to obtain the basic information on the effect of seed moisture content and cylinder speed of thresher on the mechanical damage and seed germination in soybeans. The moisture content at maturity was the highest in stern and followed by seed and pod-shell for Hwang-keurnkong and also the highest in stern and followed by pod-shell and seed for Danyeobkong in that order. The variation in the moisture contents of stern, seed, and pod-shell in a day on the 7th day after maturity showed gradually decreasing trends from 7 :00 in the morning to 17 :00 in the afternoon. On the 14th day after maturity, the moisture content of pod-shell was higher than that of seeds up until 11 :00 in the morning but it was higher in the seeds after that. The greater the cylinder speed and the higher the moisture content of seeds, the higher the percentage of seeds damaged was resulted. At the same time, the percentage of seeds damaged was higher in Hwangkeumkong large seed sized than in Danyeobkong small seed sized at the same cylinder speed. Considering the seed yield, percentage of seeds damaged, percentage of seeds germinated, threshing efficiency and drying, etc., the appropriate cylinder speed was believed to be about II m per second and the most appropriate moisture contents of seeds for threshing were believed to be about 15-20%.

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Pre-Harvest Sprouting Variation of Rice Seeds Located on Each Panicle Position According to Grain Filling Days (벼 등숙일수에 따른 이삭 착생 부위별 종자의 수발아 변이)

  • Baek, Jung-Sun;Chung, Nam-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.1
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    • pp.22-26
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    • 2014
  • This research was implemented to investigate pre-harvest sprouting characteristics in two Korean rice cultivars, Shindongjin (SDJ) and Hopum (HP). The panicles of both varieties were sampled after 15 days after heading (DAH) to 60 DAH at intervals of 5 days. As soon as sampled, the panicles were imbibed in water for 14 days at $25^{\circ}C$ to determine the vigor and germination ability of seeds according to location on panicle. To investigate the cause of non-germination of seeds in panicles, tetrazolium test and dormancy breaking were performed. The preharvest sprouting of HP started at 20 DAH while that of SDJ began at 30 DAH. The germination of seeds located in high rank branches within a panicle and the terminal seeds within a branch were earlier and faster and the germination patterns were same in both cultivars. The times at more than 50% of germination in a panicle were 35 DAH (57.0%) in HP and 45 DAH (56.8%) in SDJ. Preharvest sprouting was more than 80% at 50 DAH (82.6% of HP, 92.3% of SDJ) and more than 99% at 60 DAH (99.5%, 99.4%, HP and SDJ). These results indicated that the rate of PHS in a panicle increased with accumulation of the days after heading. The cause of non-germinated seeds at 15-25 DAH in panicle was immature embryo. After 30 DAH, however, when the non-germinated seeds were hulled, they started to germinate due to dormancy breaking, in which the germination percentage was higher in SDJ than HP. In conclusion, the pre-harvest sprouting varied according to days after heading, the seed position on panicle, and the dormancy intensity of varieties.

Evaluation of Seed Vigor and Morphology by Varietal Difference of Sorghum (Sorghum bicolor (L.) Moench) (수수의 종자형태 및 발아세 평가)

  • Jeon, Seung-Ho;Yoon, Dong-Kyung;Oh, Seung-Ka;Lee, Eun-Jung;Lee, Hyoun-Jun;Lee, Byung-Jin;Jung, Ki-Youl;Cho, Young-Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.457-462
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    • 2014
  • This study was conducted to evaluate basic seed characteristics such as morphological characteristics and various seed vigor using 4 sorghum varieties, Nampungchal, Donganme, Hwanggeumchal and Whinchal. No difference was shown in morphological characteristics of the varieties except that the width. Whinchal has bulb-shaped seed, while the other varieties have oval-shaped seeds. The seed coat color of Whinchal is obviously different from those of the other varieties; especially, Nampungchal and Hwanggeumchal are not glossy. Germination rate were Hwanggeumchal > Nampungchal > Donganme > Whinchal in the order of higher rate in standard germination tests and cold germination test. In the ageing test, Donganme showed the lowest 15% decrease in comparison with the standard cultivar. Electric conductivity (EC) has negative correlation with standard germination tests, cold germination test and accelerated ageing test. In the percentage of emergence (PE), Hwanggeumchal was the highest 85.7% which was more than 40% to the lowest Whinchal. Mean emergence time (MET) showed significant difference between the varieties; Whinchal showed the slowest 6.0 days.

Effects of Different Nitrogen Solution Varying Concentration on Seed Germination of Vegetable Crops(Brassica rapa L., Lactuca sativa L.) (농도(濃度)가 다른 여러가지 질소원(窒素源) 양분용액(養分溶液)이 배추와 상치 종자발아(種子發芽)에 미치는 영향(影響))

  • Hong, Soon-Dal;Lee, Yun-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.1
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    • pp.57-61
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    • 1992
  • For testing salts effect on seed germination, nitrogen solutions($(NH_4)_2SO_4$, $NH_4NO_3$, $KNO_3$, $Mg(NO_3)_2$, $Ca(NO_3)_2$, $(NH_2)_2CO$) were treated to chinese cabbage(Brassica rape L.) and lettuce(Lactuca sativa L.) seeding on the quartz sand bed given a tilt to wet by dripping. Electrical conductivities (mS/cm) increased with direct proportion by equivalent gram-molecular weight at $(NH_4)_2SO_4$ and $KNO_3$, $NH_4NO_3$, but those of Mg and Ca salt remained 1 1/2 times higher. And urea solution never showed EC at all. $(NH_4)_2SO_4$ solution appeared to have value of EC with 1.37 times as high as that of $KNO_3$ and it was in order of $(NH_4)_2SO_4>NH_4NO_3>KNO_3>Ca(NO_3)_2>Mg(NO_3)_2$ comparing with same percentage solution. Application of $Mg(NO_3)_2$ and $(NH_4)_2SO_4$ among 6 salts resulted in a severe decreas in germination of both crops, and $NH_4NO_3$ showed light inhibition in comparison to $KNO_3$. Urea showed little effect on germination as the same as $Ca(NO_3)_2$ and $KNO_3$ solution at low concentrations, but the latter two salts reduced germination remarkably at high concentrations. It is likely to show that germination inhibition is not affected by salt salanity but by the sort of salts.

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