• The Plant Pathology Journal
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• v.40 no.2
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• pp.205-217
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• 2024

Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicola isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.157-165
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• 2001

Amplified fragment length polymorphism(AFLP) technique is based on the polymorphism detection through selective PCR amplification of restriction fragments from digested genomic DNA and thus includes the procedures of the total DNA digestion by endonucleases, ligation of adapters to the ends of the fragments, and following the selective amplification of the restricted DNA fragments. This study were aimed to : (1) determine the genetic variability of S aureus strains, (2) estimate genetic diversity within and among these strains, (3) compare phylogenetic relationships among these strains as genetic markers using AFLP techniques. Genomic DNA was digested with a particular combination of three restriction enzymes with specific recognition sites and the DNA fragments were ligated to restriction specific adapters and amplified using the selective primer combinations. In the S aureus strain, the number of scorable AFLP bands detected per each primer combination varied from 29 to 102, with an average of 61.59 using 27 primer combinations. A total of 1,663 markers were generated, 904 bands of which were polymorphic, showing a 33.48% level of polymorphism with these primer combinations. Among the primer combinations, E02/T02, E02/T03, E04/H02, E02/T01 and E04/H03 primer combinations showed a high level of polymorphism with 0.78, 0.76, 0.74, 0.71 and 0.70, respectively. But T03/H01, E01/T02 and E01/T03 primer combinations showed a low level of polymorphism with 0.38, 0.37 and 0.15, respectively, Therefore, the former primer combinations will be the most effective for AFLP analysis of S aureus. In SA1 sub-types the level of polymorphism of S aureus KCTC 1927 was similar to that of S aureus CU 01(0.825) and higher than those of other strains such as S aureus CU 02 (0.715), S aureus KCTC 2199(0.625), S aureus KCTC 1916(0.607) and S aureus KCTC 1621 (0.553). In SA2 sub-types the level of polymorphism of S aureus CU 07 was similar to that of S aureus CU 08(0.935) and higher than those of both S aureus CU 04(0.883) and S aureus CU 05(0.883) and lower than those of S aureus CU 03(0.583). In SA3 subtypes the level of polymorphism of S aureus CU 11 was similar to that of S aureus CU 12(0.913) and lower than that of S aureus CU 15(0.623). The results proved that AFLP marker analysis of S aureus strain could be used to study the epidemiology of mastitis and in addition, common genotype in geographic region could be useful for the development of an effective vaccine or DNA marker for easy diagnosis of mastitis caused by S aureus infection.

• Journal of the Korean Association of Geographic Information Studies
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• v.11 no.4
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• pp.32-40
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• 2008

In this study, we monitor ice cap using calculated NDSI from September to December in 2001, 2003, 2006, 2007 and snow cover area in 2007 decrease by compare with 2001. Global warming is one of the most important issue in this world. Because global-warming is the reason of various meteorological disasters and extreme weather events in these days and snow and glaciers showed that global warming effect most easily. Snow and glaciers play an important role in Earth cooling system because of their high reflectance. The present study has been carried out monitoring ice cap in Himalayas, using MODIS(Moderate Resolution Imaging Spectroradiometer)data. Indicator to monitoring ice cap, NDSI(Normalized Differenced Snow Index) was used in this study. The NDSI is a spectral band ratio that takes advantage of the spectral differences of snow in visible and short-wave infrared domain to detect snow cover area versus non-snow cover area in a scene. This study is quantitative evaluation about effect of global warming for icecap.

• Journal of the Korean Association of Geographic Information Studies
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• v.15 no.1
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• pp.1-15
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• 2012

Marine scientific monitoring, including monitoring of oil pollution, marine ecosystems, and the marine environment in general, has been being carried out continuously in order to assess the impact of oil pollution since the Hebei Spirit oil spill in December 2007. GIS thematic maps containing visual and summarized information are very useful for conducting exploratory analyses on the spatio-temporal variability of the marine environment and marine ecosystems due to oil pollution. Defining map types and building a legend in accordance with data characteristics are essential elements for mapping. In the present study, map types were defined according to the data attributes and GIS data types for each data item and classification of data intervals for the legend was defined by using two data distribution types through a histogram analysis. The data interval method was defined as follows: If the histogram of data has a uniform distribution, an equal interval method is applied; in the case of a normal distribution, a standard deviation method is applied. In addition, thematic map templates were made for each map type through the definition of marginal elements. Through the establishment of systematic mapping methods and procedures in this study, it was possible to effectively make standardized thematic maps for various kinds of marine scientific data.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.4
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• pp.39-53
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• 2013

Although the efficient formation of urban space structure is a key factor in energy saving and environmentally-friendly aspect, the maintenance of the center and sub-center of the city that are key factors has been becoming increasingly difficult due to the variability and complexity of urban activities. In the case of Busan, amid the expansion of urban scale due to rapid economic development and overpopulation, systematic approaches to professional diagnosis and maintenance have been significantly insufficient - other than the city basic plan which has been conducted at an interval of 20 years. For the effective management of urban central area, systematic monitoring of the CBD through demand forecast and blight forecast at a city level must first be implemented. In order to fulfill this goal, this study is to figure out the current state of the CBD through the diagnosis on blight of the urban central area in the viewpoint of rehabilitation of the CBD and to propose the measures for practical utilization of the information about space for the further management of the central area of the city. For analysis, the study looks into the present state in terms of physical index, economic index, and social index. And then as a micro-approach by utilizing economic index, the study has thoroughly examined the economic blight of the Seomyun urban central area of Busan. The outcome of the analysis shows that in terms of population distribution and land utilization the area is in the stage of inefficient dispersion after having gone through the stage of suburbanization. It is expected that this study, as the material that proves the necessity of enhancing the function of the CBD, can propose the direction for the management of the urban center of Busan through blight prediction and management of the urban center and can provide the basic data for the long-term urban development that aims at the efficient strengthening of functions of the CBD.

• Korean Journal of Remote Sensing
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• v.20 no.2
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• pp.103-116
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• 2004

The mesoscale atmospheric models to produce surface temperature can not generally consider the effect of the sloped terrain for direct solar radiation. These have not showed the regional difference of solar radiation and as a result, have made the big error in the local surface temperature. Therefore, we wished to represent the exact locality of surface temperature by considering the geometric properties of surface as well as the vegetated properties of surface. The purpose of the study is to produce local surface ground temperature in sloped terrain diagnostically using surface Energy Balance Model (EBM) with the use of GRID model in Geographic Information Systems (GIS). In this study, surface inhomogeneity over southeastern part of Korean peninsula are considered in estimation of the absorbed surface solar radiation in terms of the illumination angle, depending on topographical aspect and slope in GRID. Also, the properties of vegetated surface which the major components for the variability of surface temperature are considered in terms of NDVI. The results of our study show the locally changes in the surface ground temperature due to local ground aspect and slope effect and local properties of vegetated surface. The more detailed distribution of local surface temperature may drive the local circulation at lower atmospheric and it may explain better the real local circulation.

• Environmental Analysis Health and Toxicology
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• v.31
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• pp.3.1-3.11
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• 2016

Objectives This study was conducted to describe the regional malaria incidence in relation to the geographic and climatic conditions and describe the effect of altitude on the expansion of malaria over the last decade in Papua New Guinea. Methods Malaria incidence was estimated in five provinces from 1996 to 2008 using national health surveillance data. Time trend of malaria incidence was compared with rainfall and minimum/maximum temperature. In the Eastern Highland Province, time trend of malaria incidence over the study period was stratified by altitude. Spatio-temporal pattern of malaria was analyzed. Results Nationwide, malaria incidence was stationary. Regionally, the incidence increased markedly in the highland region (292.0/100000/yr, p =0.021), and remained stationary in the other regions. Seasonality of the malaria incidence was related with rainfall. Decreasing incidence of malaria was associated with decreasing rainfall in the southern coastal region, whereas it was not evident in the northern coastal region. In the Eastern Highland Province, malaria incidence increased in areas below 1700 m, with the rate of increase being steeper at higher altitudes. Conclusions Increasing trend of malaria incidence was prominent in the highland region of Papua New Guinea, while long-term trend was dependent upon baseline level of rainfall in coastal regions.

• KIISE Transactions on Computing Practices
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• v.23 no.12
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• pp.660-669
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• 2017

In order to improve user accessibility and interactivity, system scalability, service speed, and a non-standard internet web environment, we developed a Web-based geovisualization system of oceanographic information using HTML5 and dynamic particles. In particular, oceanographic and meteorological data generated from a satellite remote sensing and radar measurement and a 3-dimensioanl numerical model, has the characteristics of a heterogeneous large-capacity multi-dimensional continuous spatial and temporal variability, based on geographic information. Considering those attributes, we applied dynamic particles represent the spatial and temporal variations of vector type oceanographic data. HTML5, WebGL, Canvas, D3, and Leaflet map libraries were also applied to handle various multimedia data, graphics, map services, and location-based service as well as to implement multidimensional spatial and statistical analyses such as a UV chart.

• Journal of Environmental Science International
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• v.13 no.5
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• pp.479-488
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• 2004

Five sediment cores from the tidal flat of artificial Lake Shihwa are analyzed in terms of sedimentology and geochemistry to evaluate the heavy metal contamination and redox condition of surficial sediment following the Shihwa seawall construction. The variability of concentrations of various elements depends on the depositional environment, and reflects the various redox conditions and sediment provenances. The amounts of Ti and Al and their ratio of Ti/ Al with respect to Li clearly indicate that there is an anthropogenic contribution to the surficial sediment. The high concentrations of heavy metals suggest an anthropogenic contribution at ST. 34 and ST. 22. Concentrations of most elements (Cr, Cu, Zn and Pb) are higher near the Shihwa-Banwol industrial complex than in the central part of Lake Shihwa. Concentrations of heavy metal in surficial sediment near the Shihwa-Banwol industrial complex are two to eight times higher than in the center of Lake Shihwa. Enrichment factors (EF), which are normalized by the unpolluted shale, suggests a significant metallic contamination near the Shihwa-Banwol industrial complex (SBIC). The redox condition is divided into two anoxic and mixed oxi $c_oxic zones based on the carbon:sulfur (C/S) ratios of organic matter and elemental relationships. Correlations among geochemical elements Mn, U and Mo are significantly different from site to site, and may therefore be an indicator of the spatial redox condition. Controlling factors for switching anoxic/oxic conditions are thought to be water depth and the differences in industrial effluent supply. The variations of the Cu/Mn ratio in the sediments confirms above mentioned spatial differences of a redox condition in part, and therefore shows a location-dependence redox condition in sediments at four other sites. The redox condition of the surficial sediment characteristics of the Shihwa Lake are controlled by its geographic location and water depth.th.

• Journal of the Korean Data and Information Science Society
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• v.27 no.5
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• pp.1215-1224
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• 2016

Climate information of the high resolution grid units is an important factor to explain the phenomenon in a variety of research field. Statistical linear interpolation models are computationally inexpensive and applicable to any climate data compared to the dynamic simulation method at regional scales. In this paper, we considered four different linear-based statistical interpolation models: general linear model, generalized additive model, spatial linear regression model, and Bayesian spatial linear regression model. The climate variable of interest was the daily mean temperature, where the spatial variability was explained using geographic terrain information: latitude, longitude, elevation. The data were collected by weather stations in January from 2003 and 2012. In the sense of RMSE and correlation coefficient, Bayesian spatial linear regression model showed better performance in reflecting the spatial pattern compared to the other models.