• Title/Summary/Keyword: Genus identification

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Molecular Identification and Morphological Comparison of Juveniles of Two Collichthys Species (Pisces: Sciaenidae) from the Yellow Sea (황해 강달이속(Collichthys) 치어 2종의 분자동정 및 형태비교)

  • Lee, Soo Jeong;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.1
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    • pp.79-83
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    • 2014
  • Thirty five juveniles belonging to the genus Collichthys were collected using a bag net at Gang-wha-do, in the eastern Yellow Sea, between July and September, 2012, and identified using combined genetic and morphological methods. We sequenced 316 base pairs of mitochondrial DNA cytochrome c oxidase subunit I of 35 individuals, of which 22 individuals were identified as Collichthys niveatus (12.9-47.6 mm in SL) and 13 as Collichthys lucidus (13.4-40.3 mm SL). Morphologically, the number of occipital crests, an important taxonomic character during the adult stage, could not distinguish the two species during the juvenile stage, but the shape of the first anal fin spine clearly distinguished the two species even among juveniles.

Genetic Identification of Hatchery Reared Tilapia Strains (양식 틸라피아에 대한 유전학적 동정)

  • Kim Dong Soo;Park In-Seok
    • Journal of Aquaculture
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    • v.3 no.1
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    • pp.31-37
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    • 1990
  • Ten strains of tilapiine species from genus Oreochromis were cytogenetically studied for genetic stock identification. Both the chromosome numbers(2n=44) were identical in all 10 strains. Heteromorphic sex chromosome pair were not found in any strains. Nuclear volumes vary between O. niloticus(21.0 $\mu$ $m^3$) and O. aureus(22.4 $\mu$ $m^3$)

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Molecular and Morphological Identification of a Muraenichthys gymnopterus (Ophichthidae: Anguilliformes) Leptocephalus Collected on Jeju island, Korea (한국 제주도에서 채집된 갯물뱀(뱀장어목: 바다뱀과) 엽상자어(Leptocephalus)의 형태 및 분자동정)

  • Ji, Hwan-Sung;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.5
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    • pp.507-512
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    • 2012
  • One leptocephalus (TL 59.9 mm) from the family Ophichthidae collected from Jeju Island, Korea, was identified using morphological and molecular methods. Our ophichthid leptocephalus was identified as belonging to the genus Muraenichthys based on morphological characters: 157 myomeres; the origin of the dorsal fin located a little in front of the anus; a distinct melanophore present on the opercle; and six gut swellings present. An analysis of 886 base pairs of the 12S rRNA mtDNA sequences showed that our leptocephalus must be Muraenichthys gymnopterus, because its sequences were concordant with those of an adult M. gymnopterus (d=0.001) and next to those of Muraenichthys sp. leptocephalus (d=0.034). Here, we are the first to describe the morphological characteristics of the M. gymnopterus leptocephalus.

Numerical Identification of Streptomyces fIaveus Producing Antibiotic Substances Inhibitory to Plant Pathogenic Fungi

  • Lee, Jung-Yeop;Kim, Beom-Seok;Hwang, Byung-Kook
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.324-334
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    • 1995
  • The actinomycete strain A 11 was antagonistic to plant pathogenic fungi Phytophthora capsid and Magnaporthe grisea. Based on the diaminopimelic acid (DAP) type and morphological characteristics examined by scanning electron microscopy, the strain A 11 was confirmed to belong to the genus Streptomyces. Based on Willcox probability and similarity level, the strain A 11 was numerically identified as Streptomyces flaveus using TAXON program of Ward and Goodfellow. Antibiotic production of S. flaveus strain A 11 was most favorable when cultured on glycerol yeast extract peptone (GYP) agar for 20 days at $28^{\circ}C$. The crude antibiotics from solid GYP agar cultures of the strain A 11 were most effective against Phytophthora capsici and Sclerotinia sclerotiorum among the fungi tested. Antifungal activity of the antibiotics against Alternaria solani, Botryosphaeria dothidea, Cercospora capsici, Magnaporthe grisea, and Rhizoctonia solani was somewhat high, whereas Colletotrichum gloeosporioides and Fusarium oxysporum f. sp. cucumerinum were rarely inhibited even at high concentrations.

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Species Identification of Wooden Structural Members of the Beomeo Temple

  • Eom, Young Geun;Kim, Hwa Sung;Xu, Guang Zhu
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.2 s.130
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    • pp.1-7
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    • 2005
  • Tree species of wooden structural members of the Beomeo Temple were identified based on light and scanning electron microscopic characters in the present study. Of 10 structural members, 9 softwoods and 1 hardwood were identified. Among softwood members, 7 belonged to hard pine of the Sylvestris section, and the remaining 2 to hard pine of other than the Sylvestris section and hemlock of the genus Tsuga, respectively. A single hardwood member was identified as white oak of the Prinus section under the subgenus Lepidobalanus.

Isolation and Identification of Antifungal Bacteria on Blue Mold in Apple (사과 푸른곰팡이병의 길항미생물의 분리 및 동정)

  • 이인선;조정일
    • Journal of Food Hygiene and Safety
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    • v.14 no.2
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    • pp.167-171
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    • 1999
  • In order to screen the antagonistic bacteria which inhibit the growth of the apple pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as CH142, was selected. The antagonistic strain CH142 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization. The CH142 showed 55.9% of antifungal activity against the growth of P. expansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis CH142 showed 90% and 15% of antifungal activity, respectively.

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Identification of Genus Triadinium for Plankton Monitoring Network (플랑크톤 모니터링 네트워크를 위한 Triadinium속의 동정)

  • Yeo, Hwan-Goo
    • Proceedings of the KAIS Fall Conference
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    • 2009.05a
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    • pp.842-844
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    • 2009
  • The major group which contains all the organisms that are commonly termed Dinoflagellates is called the division Pyrrophyta or Dinophyta in botanical systems and the order Dinoflagellida or Dinoflagellata in zoological systems. Most dinoflagellates are biflagellated solitary planktonic cells. A taxonomic survey of the dinoflagellate family, Triadiniaceae Dodge was conducted for plankton monitoring network in the coastal waters of Korea. Identification of species in water samples was usually done by using differential interference contrast (DIC), which revealed especially well lighted thecal structures. For the apparent three-dimensional image, scanning electron microscope (SEM) was used. Two species were identified and described, which were Triadinium orientale(Lindemann)Dodge and Triadinium sphaericum(Murray and Whitting)Dodge.

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Studies on Koji for Optimum Conditions of Growth and Idenfication of Aspergillus spp.(Part2) (장류용 강력국균 연구 2)

  • 이계호;장건형
    • Korean Journal of Microbiology
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    • v.2 no.1
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    • pp.17-18
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    • 1964
  • Ke Ho Lee and Kun Hyung Chang (Army Research and Testing Lab.): Studies on koji for optimum condition of growth and identification of Aspergillus spp. Cpart 2). Kor. J. Microbiol. Vol. 2, No. 1, p. 17-18 (1964). The experiments reported in this paper are concerned with the identifications, the growth conditions and lethal temperatures of the three strains of Aspergillus spp. which have been isolated from the soils and Meju (fermented soy bean in Korea). The results obtained in the light of the manual of Raper and Thom for identification of the Genus, Aspergillus, have been shown that the three strains of Aspergillus spp. are pertained to Aspergillus flavusoryzae group. The optimum temperature, pH and the lethal temperatures of the Aspergillus spp. have been measured.

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Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

  • Liu, Zhigao;Korpelainen, Helena
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.889-897
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    • 2018
  • Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.

New Record of Sillago sinica (Pisces: Sillaginidae) in Korean Waters, and Re-identification of Sillago parvisquamis Previously Reported from Korea as S. sinica

  • Bae, Seung Eun;Kwun, Hyuck Joon;Kim, Jin-Koo;Kweon, Seon-Man;Kang, Chung Bae
    • Animal Systematics, Evolution and Diversity
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    • v.29 no.4
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    • pp.288-293
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    • 2013
  • A single specimen of the genus Sillago, collected from Gwangyang, Korea, in May 2009, is characterized by XI first dorsal fin spines, 3 or 4 rows of melanophore pattern along the second dorsal fin membrane, and a darkish posterior margin of the caudal fin. Our specimen was identified as Sillago sinica reported as a new species; this identification is confirmed by mitochondrial DNA cytochrome oxidase subunit I sequences, which show that our specimen corresponds to S. sinica (d=0.000) and differs from the congeneric species Sillago parvisquamis (d=0.170). Comparisons of Korean specimens previously reported as S. parvisquamis with specimens of S. sinica show that the S. parvisquamis specimens are actually S. sinica. We propose the new Korean name "buk-bang-jeom-bo-ri-myeol" for S. sinica.