• Title/Summary/Keyword: Genomics

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Diversity and Polymorphism in AHL-Lactonase Gene (aiiA) of Bacillus

  • Huma, Nusrat;Shankar, Pratap;Kushwah, Jyoti;Bhushan, Ashish;Joshi, Jayadev;Mukherjee, Tanmoy;Raju, Sajan C.;Purohit, Hemant J.;Kalia, Vipin Chandra
    • Journal of Microbiology and Biotechnology
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    • v.21 no.10
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    • pp.1001-1011
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    • 2011

  • To explore bacterial diversity for elucidating genetic variability in acylhomoserine lactone (AHL) lactonase structure, we screened 800 bacterial strains. It revealed the presence of a quorum quenching (QQ) AHL-lactonase gene (aiiA) in 42 strains. These 42 strains were identified using rrs (16S rDNA) sequencing as Bacillus strains, predominantly B. cereus. An in silico restriction endonuclease (RE) digestion of 22 AHL lactonase gene (aiiA) sequences (from NCBI database) belonging to 9 different genera, along with 42 aiiA gene sequences from different Bacillus spp. (isolated here) with 14 type II REs, revealed distinct patterns of fragments (nucleotide length and order) with four REs; AluI, DpnII, RsaI, and Tru9I. Our study reflects on the biodiversity of aiiA among Bacillus species. Bacillus sp. strain MBG11 with polymorphism (115Alanine > Valine) may confer increased stability to AHL lactonase, and can be a potential candidate for heterologous expression and mass production. Microbes with ability to produce AHL-lactonases degrade quorum sensing signals such as AHL by opening of the lactone ring. The naturally occurring diversity of QQ molecules provides opportunities to use them for preventing bacterial infections, spoilage of food, and bioremediation.

  • https://doi.org/10.4014/jmb.1105.05056 인용 PDF KSCI

Korea Brassica Genome Sequencing Project

  • Kwon, Soo-Jin;Yang, Tae-Jin;Kim, Jung-Sun;Kim, Jin-A;Jin, Min-A;Park, Jee-Young;Choi, Beom-Soon;Lim, Myung-Ho;Lee, Soo-In;Lee, Sang-Choon;Kim, Ho-Il;Lim, Ki-Byung;Lim, Yong-Pyo
    • The Korea Genome Conference
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    • 2006.09a
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    • pp.27-27
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    • 2006

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Flowering time genes in Brassica genome

  • Kwon, Soo-Jin;Yang, Tae-Jin;Kim, Jung-Sun;Lim, Ki-Byung;Kim, Jin-A;Lim, Myung-Ho;Jin, Mi-Na;Park, Jee-Young;Kim, Ho-Il;Park, Beom-Seok
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2005.11a
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    • pp.396-396
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    • 2005

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Comparative analysis of expressed sequence tags of Brassica rapa

  • Lim, Myung-Ho;Kim, Jin-A;Lee, Ji-Young;Park, Yang-Sun;Park, Beom-Soon;Seol, Young-Joo;Kim, Jung-Sun;Jin, Mi-Na;Lim, Ki-Byung;Yang, Tae-Jin;Kim, Ho-Il;Lim, Chae-Oh;Chung, Yong-Yoon;Hur, Yoon-Kang;Park, Beom-Seok
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2005.11a
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    • pp.340-340
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    • 2005

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