• 대한병원협회지
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• 제30권4호통권272호
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• pp.4-8
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• 2001

• 대한가정학회지
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• 제40권11호
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• pp.83-91
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• 2002

This study examined the consumers' willingness to purchase Genetically Modified Organism(GMO) and the factors to distinguish subjects with differential levels of their willingness to buy GMO. Data were collected from 506 male and female adults in Seoul, Daegu and Susan by the self-administered questionnaire. Frequencies, X²tests, F tests and discriminant analysis were conducted to analyze the data by SPSS Windows. The results of this study were as follows: (1) the concerns and the information-seeking behavior about GMO were generally high, but recognition of GMO was low, (2) the willingness to purchase GMO was generally high in that 2/3 of the subjects were willing to purchase it, and (3) the concerns about GMO, the information-seeking behavior, age and sex were the factors to distinguish acceptors from non-acceptors.

• 대한가정학회지
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• 제40권4호
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• pp.73-84
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• 2002

This study found out the consumers' awareness and information-seeking behaviors towards Genetically Modified Organism(GMO). Data were collected from 506 adults in Seoul, Daegu, and Busan by the self-administered questionnaire. Frequencies, Pearson's correlation and multiple regression analyses were conducted by SPSS Windows. The resets of this study were as follows: (1) the concerns and the need for information about GMO were high, but recognition was low, (2) the factor affecting the need for information about GMO was the concerns about GMO, and (3) sex, age, the need for information about GMO, and subscription experience of consumer journals were the factors affecting the information-seeking behaviors.

• 농업과학연구
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• 제46권4호
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• pp.705-713
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• 2019

This study was conducted to determine the effects of dietary non-genetically modified organism (non-GMO) wheat-based diets on the growth performance, nutrient digestibility, blood profile and meat quality of grower-finisher pigs. A total of 70 [(Landrace × Yorkshire) × Duroc] growing pigs with an initial body weight of 26.15 ± 1.57 kg were used in a 112 day trial. The dietary treatments were as follows: (I) CD, corn-based diet and (II) non-GMO WD, a non-genetically modified organism wheat-based diet. Each treatment consisted of 7 replicate pens with 5 pigs per pen. In the current study, the pigs fed the corn-based diet had a higher body weight than the pigs fed the non-GMO wheat-based diet at day 21 and day 77 (p < 0.05). There was a significant difference in the average daily gain (ADG) during the first 21 days (p < 0.05). The non-GMO wheat-based diet had no effect on nutrient digestibility. In addition, the non-GMO wheat-based diet had no effect on the blood profile except for blood urea nitrogen (BUN) at d 21. In conclusion, the non-GMO wheat-based diet only had a slight effect on the growth performance of growing pigs but had no significant impact on the nutrient digestibility, fecal score, blood profile and meat quality of the pigs during the grower-finisher period.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제1권1호
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• pp.83-89
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• 2020

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

• 농업과학연구
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• 제45권1호
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• pp.28-37
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• 2018

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1150-1156
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• 2004

The GMO (Genetically Modified Organism) labeling system on raw materials has been in Korea since March 2001, and genetically modified organisms (GMOs)-derived foods since July 2001. Therefore, we designed a multiplex PCR method to ascertain the validity of the labeling system and to monitor the status of circulation for genetically modified maize (GM Maize). Five lines of GM Maize (GA21, TC1507, Mon810, NK603, and Bt176) were used, and specific primer pairs were designed to detect each line. Using this method, the different lines of GM Maize were monitored from raw products and processed foods in Korean market. Some of the maize processed foods and raw materials were shown to contain more than one foreign gene. This method was found to be effective for-detecting five different GM Maize in a single reaction.

• 한국생활과학회지
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• 제12권3호
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• pp.351-362
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• 2003

The goal of this study was to examine the effects of perceived risks toward GMO (Genetically Modified Organism) food on the decision-making to purchase it and demands to reveal its Identity. The major results were as follows. First, consumers strongly perceived the risk toward GMO food, and demanded labeling to identify it. But, many consumers had indecisive in the purchase of GMO food. Second, consumers with high level of perceived risks toward GMO food were more likely not to buy GMO food. In addition, the level of perceived risk was high among consumers who had many children. Finally, consumers with high level of perceived risks toward GMO food were more likely to demand the labelling for identifying GMO food. Consumers who had many children and high level of reliability for labelling food safety strongly demanded to label for revealing GMO food.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.309-315
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• 2020

Advances in biotechnology have led to progress in crop genetic engineering to improve agricultural productivity. The use of genetically modified (GM) crops has increased, as have consumers' and regulators' concerns about the safety of GM crops to human health, and ecological biodiversity. As such, the identification of GM crops is a critical issue for developers and distributors, and their labeling is mandatory. Multiplex polymerase chain reaction (PCR) has been developed and its use validated for the detection and identification of GM crops in quarantine. Herein, we established a simultaneous detection method to identify four GM maize events. Event-specific primers were designed between the junction region of transgene and genome of four GM maize lines, namely 5307, DAS-40278-9, MON87460, and MON87427. To verify the efficiency and accuracy of the multiplex PCR we used specificity analysis, limit of detection evaluation, and mixed certified reference materials identification. The multiplex PCR method was applied to analyze 29 living, modified maize volunteers collected in South Korea in 2018 and 2019. We performed multiplex PCR analysis to identify events and confirmed the result by simplex PCR using each event-specific primer. As a result, rather than detecting each event individually, the simultaneous detection PCR method enabled the rapid analysis of 29 GM maize volunteers. Thus, the novel multiplex PCR method is applicable for living modified organism volunteer identification.

• 대한지역사회영양학회지
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• 제15권5호
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• pp.679-686
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• 2010

The purpose of this study is to monitor genetically modified bean sprouts at traditional markets in Seoul and to investigate perception of traditional market merchants and high school students on genetically modified organisms (GMOs). We analyzed 30 bean sprouts that were selected at 11 traditional markets in Seoul using the method of polymerase chain reaction (PCR). Also, we compared perception of GMOs between merchants (n = 30) and students (n = 126). Knowledge test about GMOs was performed by students. The result of PCR, 16.7% of bean sprouts were confirmed as GM bean sprouts (n = 5). Students had significantly more exposure to information about GMOs than merchants (p < 0.05). Major information sources about GMOs were from mass media (television, newspaper and radio). About half of subjects were not aware that they eat GMOs and GMOs are sold to consumers. Only 17.3% of subjects had constant eating intent for GMOs after perceiving foods that he/she usually eats are GMOs. 51.3% of subjects had willingness to purchase GMOs if GMOs have same quality and lower cost than natural foods. 37.2% of subjects thought that GMOs would be harmful to humans. Students had more positive perception of GMOs' side effects than merchants (p < 0.01). There was no merchant who knew labeling of GMOs. 19.1% of students knew labeling of GMOs. Students' mean percentage of correct answers of six questions about GMOs was 45.2%. Therefore, providing adequate information about GMOs is needed for consumer's choice whether to purchase GMOs or not.