• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.93-97
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• 1998

The results of chromosome aberration test in mammalian cells in culture (Chinese hamster lung fibroblast cells) showed no induction of structural and numerical aberrations by antifungal agents of YH1715 series regardless of metabolic activation. While positive control group (mitomycin C and benzo(a)pyrene) showed structural chromosome aberrations of 37% and 23%, respectively. The in vivo induction of micronuclei was measured in polychromatic erythrocytes in bone marrow of male ddY mouse given YH1715R and YH1729R at 1, 0.5, 0.25 g/kg by p.o. once. After 24 hours, animals were sacrificed and evaluated 40 the incidence of micronucleated polychromatic erythrocytes in whole erythrocytes. Although a positive response for induction of micronuclei in animals treated with mitomycin C demonstrated the sensitivity of the test system for detection of a chemical clastogen, YH1715R did not induce micronuclei in bone marrow of ddY male mice but induced cytotoxicity to bone marrow cells at the highest concentration (1 g/kg, p〈0.05), and YH1729R induced micronuclei in bone marrow of ddY male mice dose dependently (p<0.05) but did not induce cytotoxicity to bone marrow cells.

• The Plant Pathology Journal
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• 제17권4호
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• pp.227-230
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• 2001

Bioassay techniques using young leaves and roots were developed to screen resistance of Atractylis spp. against Phytophthora drechsleri. Among 638 plants collected from various regions of Korea from 1994 to 1996, 67 were pre-screened in fields naturally infested with P. drechsleri, which is the causal pathogen of rhizome rot of Atractylis. Among the pre-screened sources, 18 (ca. 26.8%) were highly resistant to the pathogen in leaf inoculation. In the root inoculation test, abundant sporangia were formed in susceptible plant roots, while only a few or no sporangia were produced on the roots which were found resistant in the leaf inoculation test. Among the selected resistant plants, A. japonica 96066 and 96104 were used to cross with another species, A. macrocephala 96362 that showed high yield with good quality of rhizome but susceptible to the pathogen. The F$_1$hybrids designated as HA03 turned out to be resistant to the pathogen, indicating that resistant gene(s) was inherited. Among intra-species hybrids of A. japonica, HA07 and HA09 were resistant to the pathogen in leaf inoculation and moderate in root inoculation. However, HA08 was susceptible in both inoculation tests. This result suggests that the parent material might be genetically heterogeneous. Further genetic study should be carried out to verify this phenomenon.

• Smart Structures and Systems
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• 제11권1호
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• pp.53-67
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• 2013

In this paper we apply Monte Carlo Filter to identifying dynamic parameters of structural systems and improve the efficiency of this algorithm. The algorithms using Monte Carlo Filter so far has not been practical to apply to structural identification for large scale structural systems because computation time increases exponentially as the degrees of freedom of the system increase. To overcome this problem, we developed a method being able to reduce number of particles which express possible structural response state vector. In MCF there are two steps which are the prediction and filtering processes. The idea is very simple. The prediction process remains intact but the filtering process is conducted at each node of structural system in the proposed method. We named this algorithm as relaxation Monte Carlo Filter (RMCF) and demonstrate its efficiency to identify large degree of freedom systems. Moreover to increase searching field and speed up convergence time of structural parameters we proposed an algorithm combining the Genetic Algorithm with RMCF and named GARMCF. Using shaking table test data of a model structure we also demonstrate the efficiency of proposed algorithm.

• 한국작물학회지
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• 제47권1호
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• pp.36-41
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• 2002

Two rice varieties, 'Oochikara' with large grain and 'Hwayeongbyeo' and their progenies (F$_1$, F$_2$, B$_1$ and B$_2$) were tested to understand gene action of morphological traits of rice grain and their relationships. The evaluated traits were 1,000-grain weight, grain length, width, thickness, length-width ratio and chalkiness of brown rice. Correlation between grain weight and chalkiness was highly significant in the all progenies, and grain length were not associated with width and thickness in an F$_2$ population. Scaling test and jonit scaling test revealed that inheritance of grain traits were fitted to additive-dominance model without epistasis. Additive effects for the traits were much greater than the dominance effects.

• ALGAE
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• 제24권3호
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• pp.129-138
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• 2009

DNA-based discrimination of species is a powerful way for morphologically otherwise similar species, like centric diatoms. Here, the author sequenced long-range nuclear ribosomal DNAs, spanning from the 18S to the D5 region of the 28S rDNA, of Stephanodiscus, particularly including a Korean isolate. By comparisons, high DNA similarities were detected from the rDNAs of nine Stephanodiscus (>99.4% in 18S rDNA, >98.0% in 28S rDNA). Their genetic distances, however, were significantly different (Kruskal-Wallis test, p < 0.01) compared to two related genera, namely Cyclotella and Discostella. In addition, genetic distances of 18S rDNAs were significantly different (Student’s t-test, p = 0.000) against those of the 28S rDNAs according to individual genera (Cyclotella, Discostella, and Stephanodiscus). Phylogenetic analyses showed that Stephanodiscus and Discostella showed a sister taxon relationship, and their clade was separated from a cluster of Cyclotella (1.00 PP, 100% BP). This suggests that Stephanodiscus has highly conserved sequences of both 18S and 28S rDNA; however, Stephanodiscus is well-separated from other freshwater centric diatoms, such as Cyclotella and Discostella, at the generic level.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 하계학술대회 논문집 Vol.4 No.1
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• pp.58-62
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• 2003

Silicon carbide (SiC) was etched in a $NF_3/CH_4$ inductively coupled plasma. The etch process was modeled by using a neural network called generalized regression neural network (GRNN). For modeling, the process was characterized by a $2^4$ full factorial experiment with one center point. To test model appropriateness, additional test data of 16 experiments were conducted. Particularly, the GRNN predictive capability was drastically improved by a genetic algorithm (GA). This was demonstrated by an improvement of more than 80% compared to a conventionally obtained model. Predicted model behaviors were highly consistent with actual measurements. From the optimized model, several plots were generated to examine etch rate variation under various plasma conditions. Unlike the typical behavior, the etch rate variation was quite different depending on the bias power Under lower bias powers, the source power effect was strongly dependent on induced dc bias. The etch rate was strongly correated to the do bias induced by the gas ratio. Particularly, the etch rate variation with the bias power at different gas ratio seemed to be limited by the etchant supply.

• 한국동물학회지
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• 제20권4호
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• pp.159-168
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• 1977

계대 배양중인 생쥐의 임파종양 L5178Y 세포의 유전자돌연변이 유발성 검출법(Methotrexate-저항성)과 순수분리한 사람의 임파구에서의 DNA 회복복제법을 사용하여 Salmomella/microsome 시스템에서 돌연변이 유발성이 확인된 살충데 DDVP와 trichlorfon, 살균제 TMTD 및 제초제 MO와 NIP등 5종의 농약이 포유동물 세포에 미치는 유전적 영향을 조사했다. 조사한 농약중 TMTD는 상기조사한 시스템 모두에서 양성결과를 보여준 반면에 DDVP와 trichlofon은 L5178Y 세포에서의 돌연변이 유발성은 나타내지 않았으나 DNA회복 복제법에서는 양성결과를 보여주었다. MO와 NIP는 조사한 시스템 모두에서 양성결과를 나타내지않았다.

• Nuclear Engineering and Technology
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• 제39권1호
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• pp.63-74
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• 2007

In this paper, a reconfigurable controller consisting of a normal controller and a standby controller is designed to control the thermoelectric (TE) power in the SP-100 space reactor. The normal controller uses a model predictive control (MPC) method where the future TE power is predicted by using support vector regression. A genetic algorithm that can effectively accomplish multiple objectives is used to optimize the normal controller. The performance of the normal controller depends on the capability of predicting the future TE power. Therefore, if the prediction performance is degraded, the proportional-integral (PI) controller of the standby controller begins to work instead of the normal controller. Performance deterioration is detected by a sequential probability ratio test (SPRT). A lumped parameter simulation model of the SP-100 nuclear space reactor is used to verify the proposed reconfigurable controller. The results of numerical simulations to assess the performance of the proposed controller show that the TE generator power level controlled by the proposed reconfigurable controller could track the target power level effectively, satisfying all control constraints. Furthermore, the normal controller is automatically switched to the standby controller when the performance of the normal controller degrades.

• 한국약용작물학회지
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• 제17권3호
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• pp.198-203
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• 2009

Isatis tinctoria var. yezoensis (Ohwi) Ohwi (Cruciferae) is one of major natural dyeing crops in the world and also have used as a medicinal plant in Korea. We evaluated seed purity in $F_1$-hybrid accessions using amplified fragment length polymorphism (AFLP) markers. One hundred sixty seeds from the male and female harvests were subsequently screened for seed purity with ten primers. The 13 accession-specific bands and many variable AFLP bands scored for accessions. Especially, E-AAC/M-CAA and E-AAG/M-CAT were presented clear hybrid bands for $F_1$ hybrids. $F_1$ hybrids maintained higher average level of genetic diversity compared with their correspondent parents. Self-inbred seeds from the female and male harvests were revealed 8.0% and 5.0%, respectively. The AFLP may lead to a better insight in to the hybrid seed purity test in I. tinctoria var. yezoensis.

• Journal of Genetic Medicine
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• 제18권1호
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• pp.31-37
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• 2021

Purpose: To summarize the results of chromosomal microarray analysis (CMA) for copy number variants (CNVs) detection and clinical utility in a single tertiary hospital. Materials and Methods: We performed CMA in 46 patients over the course of two years. Detected CNVs were classified into five categories according to the American College of Medical Genetics and Genomics guidelines and correlated with clinical manifestations. Results: A total of 31 CNVs were detected in 19 patients, with a median CNV number per patient of two CNVs. Among these, 16 CNVs were classified as pathogenic (n=3) or likely pathogenic (LP) (n=11) or variant of uncertain significance (n=4). The 16p11.2 deletion and 16p13.11 deletion classified as LP were most often detected in 6.5% (3/46), retrospectively. CMA diagnostic yield was 24.3% (9/37 patients) for symptomatic patients. The CNVs results of the commercial newborn screening test using next generation sequencing platforms showed high concordance with CMA results. Conclusion: CMA seems useful as a first-tier test for developmental delay with or without congenital anomalies. However, the classification and interpretation of CMA still remained a challenge. Further research is needed for evidence-based interpretation.