• Animal Systematics, Evolution and Diversity
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• 제33권3호
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• pp.169-175
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• 2017

In the northwestern Pacific region, the pen shell (Atrina pectinata) is a widely distributed bivalve and economically important in fisheries. Recently, stock of this species has been greatly reduced due to overexploitation and marine pollution, which arouses interest in conservation. Studies on genetic and taxonomic entities of pen shells have not been tried in Korea, which makes difficult to take measures for effective conservation of this marine resource. In this study, we investigated mitochondrial genetic polymorphism of pen shells collected from 4 locations in Korea and Japan using cytochrome c oxidase I (COI) gene sequences. A total of 39 haplotypes were identified among 86 individuals of pen shell. Although only 5 haplotypes were shared, no significant genetic differentiation was observed between Korean and Japanese populations. These results suggest that pen shell populations of these regions share an ancestral population which might have experienced expansion during the Pleistocene, but gene flow must have been highly restricted after expansion.

• 한국자원식물학회지
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• 제22권6호
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• pp.571-583
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• 2009

Lam Dong Province of Vietnam has an exceptional diversity of orchids and herbs. At least 920 herb species are widely mentioned in Vietnamese traditional medicinal literature and over 239 Lam Dong wild orchid species are used for attention under the Conservation Vietnamese wild orchids projects. In collaboration with Department of Plant Biotechnology, Dongguk University - South Korea working under supported ODA funds from Korea government (MIFAFF), we will consider how to collecting and preserving these plants in situ or conservating in vitro as a genetic resource.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.482-485
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• 2006

Korean native goats have lived on the Korean peninsula for more than 2,000 years and are regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 19 Korean native goats were grouped into six haplotypes and the large majority of haplotypes were present in 13 animals. All mtDNA of these Korean goats belonged to the mitochondrial (mt) lineage A and revealed remarkably small genetic distances within the population when compared with other Asian goat populations, indicating less genetic variation in the Korean native goats. These results indicate high-inbred status of the Korean native goats and will influence breeding and conservation strategies adopted for this breed.

• Molecules and Cells
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• 제26권3호
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• pp.314-318
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• 2008

Korean long-tailed goral (Nemorhaedus caudatus) is one of the most endangered species in South Korea. However, detailed species distribution and sex ratio data on the elusive goral are still lacking due to difficulty of identification of the species and sex in the field. The primary aim of this study was to develop an economical PCR-RFLP method to identify species using invasive or non-invasive samples from five Korean ungulates: goral (N. caudatus), roe deer (Capreolus pygargus), feral goat (Capra hircus), water deer (Hydropotes inermis) and musk deer (Moschus moschiferus). The secondary aim was to find more efficient molecular sexing techniques that may be applied to invasive or non-invasive samples of ungulate species. We successfully utilized PCR-RFLP of partial mitochondrial cytochrome b gene (376 bp) for species identification, and sex-specific amplification of ZFX/Y and AMELX/Y genes for sexing. Three species (goral, goat and water deer) showed distinctive band patterns by using three restriction enzymes (Xbal, Stul or Sspl). Three different sexing primer sets (LGL331/335 for ZFX/Y gene; SE47/48 or SE47/53 for AMELX/Y gene) produced sex-specific band patterns in goral, goat and roe deer. Our results suggest that the molecular analyses of non-invasive samples might provide us with potential tools for the further genetic and ecological study of Korean goral and related species.

• Journal of Ecology and Environment
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• 제43권4호
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• pp.454-461
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• 2019

Background: Ikonnikov's whiskered bat (Myotis ikonnikovi) is found throughout the Korean Peninsula, as well as in Kazakhstan, Russia, Mongolia, China, and Japan. It is small-sized and primarily inhabits old-growth forests. The decrease and fragmentation of habitats due to increased human activity may influence the genetic structure of bat populations. This study was designed to elucidate the population genetic structure of M. ikonnikovi using mitochondrial genes (cytochrome oxidase I and cytochrome b). Results: The results showed that M. ikonnikovi populations from Korea have high genetic diversity. Although genetic differentiation was not detected for the COI gene, strong genetic differentiation of the Cytb gene between Mt. Jeombong and Mt. Jiri populations was observed. Moreover, the results indicated that the gene flow of the maternal lineage may be limited. Conclusions: This study is the first to identify the genetic population structure of M. ikonnikovi. We suggest that conservation of local populations is important for sustaining the genetic diversity of the bat, and comprehensive studies on factors causing habitat fragmentation are required.

• 한국자원식물학회지
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• 제30권2호
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• pp.219-239
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• 2017

본 연구는 국립용현자연휴양림일대 관속식물상을 밝히고 주요 식물의 분포를 파악하고자 2014년 2월부터 2014년 9월까지 총 7회에 걸쳐 조사를 실시하였다. 관속식물은 95과 280속 398종 3아종 50변종 9품종의 총 460분류군이며, 이 중 한국 특산식물은 총 6분류군, 산림청 지정 희귀식물은 8분류군이 조사되었다. 식물구계학적 특정식물종은 IV등급 7분류군, III등급 7분류군, II등급 12분류군, I등급에는 17분류군 등 총 43분류군이 분포하고 있었다. 귀화식물은 39분류군으로 확인되었으며, 귀화율은 8.5%로 나타났다. 조사된 470분류군의 유용도는 식용 189분류군(41.1%), 약용 155분류군(33.7%), 목초용 177분류군(38.5%), 관상용 52분류군(11.3%), 목재용 17분류군(3.7%), 섬유용 12분류군(2.6%), 공업용 3분류군(0.7%)로 나타났다. 본 연구지역은 식생은 양호하나 인위적인 간섭에 의한 훼손이 일어나고 있으며, 장기모니터링을 통해 관속식물상과 식생 동태파악이 이루어져야 할 것으로 판단된다.

• 한국습지학회지
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• 제24권1호
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• pp.1-24
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• 2022

본 연구는 국내 산림에 분포하는 습원을 대상으로 유형을 구분한 후 평가체계에 따라 등급을 부여하여 효율적인 보전 및 관리 체계를 구축하고자 2015년부터 2019년까지 수행되었다. 2005년부터 2014년까지 제 1차 전국 산림습원 조사를 통해 도출된 1,264개소와 추가로 발굴된 16개소 총 1,280개소의 산림습원 중 습원의 기능과 가치가 있는 455개소를 구분하여, 평가를 진행하였다. 산림습원은 자연형, 묵논형, 인공형, 변형 등 4개 유형으로 구분되고, 이를 세부적으로 재분류하여, 총 11개의 세부적 습지 유형으로 구분할 수 있었다. 이를 토대로 식물 및 생태, 수문·수리, 인문사회, 교란정도 등 다양한 항목에 따라 평가한 후 등급화한 결과, 산림습원의 가치가 매우 높은 A 등급은 30개소, 비교적 높은 수준의 가치를 지닌 B등급 201개소, 보통 수준의 가치인 C등급 184개소, 낮은 수준의 가치인 D등급 40개소를 확인하였다. 평가에서 A 또는 B로 높은 등급을 받은 231개소에 대해 산림유전자원보호구역과 기타 효과적인 지역 기반 보전 수단을 지정하고, 또한 장기적인 모니터링 체계 시스템을 구축하여 산림생물다양성의 핫스팟 지역인 산림습원을 범국가적인 차원에서 보다 더 효과적이고 체계적으로 관리할 수 있을 것으로 판단된다.

• Animal Systematics, Evolution and Diversity
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• 제29권2호
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• pp.136-143
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• 2013

The collared scops owl that occurs in Korea is a protected species but its exact specific status has been questioned. To resolve the species status, a molecular phylogenetic analysis was conducted using two fragments of mitochondrial DNA, cytochrome b (cyt b, 891 bp) and NADH dehydrogenase subunit 2 (ND2, 627 bp) genes. Phylogenetic trees of cyt b revealed that all Korean specimens formed a monophyletic group with Japanese scops owl Otus semitorques with very low sequence divergence (d=0.008). We obtained a similar ND2 tree as well (d=0.003); however, the genetic distance between Korean individuals and O. lempiji from GenBank (AJ004026-7, EU348987, and EU601036) was very high and sufficient enough to separate them as species (cyt b, d=0.118; ND2, d=0.113). We also found that Korean species showed high differentiation from O. bakkamoena (AJ004018-20 and EU601034; cyt b, d=0.106; ND2, d=0.113) and O. lettia (EU601109 and EU601033, cyt b, d=0.110; ND2, d=0.117) as well. Therefore, we suggest that the Korean collared scops owl should be designated as Otus semitorques.

• Journal of Ginseng Research
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• 제28권1호
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• pp.60-66
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• 2004

“The Regional complex long-term program of restoration (reintroduction) of Primoryes ginseng population up to 2005” elaborated by Primorye governor administration, Regional Committee of Natural Resources and Russian Academy of Sciences operates in Russian Primorye. The Institute of Biology and Soil Science (IBSS) provides the scientific implementation of the program including the genetic analysis of extant ginseng populations, plant reproduction and off-spring identification. According to our investigations, the genetic resource of P. ginseng in Primorye is represented by three populations of wild-growing ginseng and a few pritate plantations. The results obtained by RAPD allowed concluding that the resource is dispersed among the wild and cultivated ginseng sub-populations in such a way that each of sub-populations studied has to be represented as a stock material to maintain species genetic variability. The allozyme analyses also showed that the small sub-populations of wild ginseng are characterized by unique genetic diversity and, therefore, they all need to be represented in reintroduction centers. Additionally the allozyme analysis discovered that the Blue Mountain and Khasan populations possess the most genetic diversity. So, at least one more reproductive ginseng unit has to be created besides two already existing reintroduction centers representing the Sikhote-Alin and the Blue Mountain populations.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.509-521
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• 2002

'The Regional complex long-term program of restoration (reintroduction) of Primoryes ginseng population up to 2005' elaborated by Primorye governor administration, Regional Committee of Natural Resources and Russian Academy of Sciences operates in Russian Primorye. The Institute of Biology and Soil Science (IBSS) provides the scientific implementation of this program including the genetic analysis of extant ginseng populations, plant reproduction and offspring identification. According to our investigations, the genetic resource of P. ginseng in Primorye is represented by three populations of wild-growing ginseng and a few private plantations. The results obtained by RAPD allowed concluding that this resource is dispersed among the wild and cultivated ginseng sub-populations in such a way that each of sub-populations studied has to be represented in living plant collection as a stock material to maintain species genetic variability. The allozyme analyses also showed that the small sub-populations of natural ginseng are characterized by unique genetic diversity and, therefore, they all need to be represented in reintroduction centers. Additionally the allozyme analysis discovered that the Blue Mountain and Khasan populations possess the most genetic diversity. So, at least one more reproductive ginseng unit has to be created besides two already existing reintroduction centers representing the Sikhote-Alin and the Blue Mountain populations.