• Current Research on Agriculture and Life Sciences
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• 제20권
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• pp.77-82
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• 2002

This study was carried out in order to produce useful material for the forest multiple use and forest protection by physico-chemical soil analysis of studied area in Sokwang-ri Forest Genetic Resource Protection Forest which was divided into in standard plots include Tricholoma matsutake and Sarcodon aspratus production forest. The result of physico-chemical soil analysis represented as following. The soil type of T. matsutake production forest was Dry brown forest soil(B1), while on the other hand the soil type of S. aspratus production forest was Moderately moist brown forest soil(B3). Between T. matsutake and S. aspratus production forest did not result in significant changes in soil pH(5.22-5.60) and soil depth(47cm), but available phosphorus, carbon, and nitrogen contents were different results. CN ratio of the fairy ring of T. matsutake was quite lower than that in S. aspratus production forests, which indicated that T. matsutake production forest was built up in the relatively immature soils which contain little organic matter. Generally, it was predicted that Pinus densiflora for. erecta forest succeeded to deciduous tree forest in stable soil environments. To conserve these T. matsutake and S. aspratus production forest, the contents of available phosphorous and exchangeable cation should be increased by continuous soil environment management and it should be established the secondary growth forests of old aged Pinus densiflora for. erecta trees as soon as possible.

• 정보과학회 논문지
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• 제42권7호
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• pp.878-888
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• 2015

소프트웨어 프로젝트 계획은 각 작업의 공수를 예측하고, 적절한 인력을 할당한 뒤 일정을 산출하는 과정을 포함한다. 프로젝트의 규모가 커질수록 가능한 개발 계획의 경우의 수가 많아지기 때문에 관리자가 합리적인 결정을 내리기 어렵다. 이를 지원하기 위해 프로젝트 계획 생성 기법들이 제안되어왔다. 제안된 대부분의 기법들은 전체 프로젝트 일정의 최소화를 계획 생성의 가장 중요한 목표로 사용한다. 하지만 일정 최소화를 달성하는 계획은 개발되는 소프트웨어의 품질을 고려하지 못하기 때문에 이러한 부분을 함께 반영한 프로젝트 계획 생성이 필요하다. 본 연구에서는 합리적인 프로젝트 계획을 생성하기 위하여 소프트웨어 프로젝트 계획에 따른 소프트웨어 품질 점수를 정의하고 동시에 전체 일정 목표를 고려할 수 있는 프로젝트 계획 생성 기법을 제안한다. 관리자는 본 기법을 사용하여 전체 소프트웨어 개발 프로젝트에 요구되는 기한 안에 해당 소프트웨어의 품질을 최대하는 계획을 생성할 수 있다.

• 한국육종학회지
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• 제41권3호
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• pp.205-212
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• 2009

Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).

• 한국버섯학회지
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• 제16권4호
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• pp.334-337
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• 2018

유버섯자원 개발을 위한 기초자료를 확보하기 위해용용 흰가시광대버섯(A. virgineoides)의 정점조사지역 내 발생양상과 기온의 변화양상을 조사하였다. 버섯발생전 60일간의 기온변화는 조사지역에 따라 다르게 나타났으며, 특히 발생전 20일간의 기온분포는 발생여부와 조사지역에 따라 상반되는 양상을 보였다. 흰가시광대버섯은 활물공생균으로 기온, 강수량, 상대습도 등과 같은 기상환경뿐만 아니라, 식물분포, 토양미생물, 토양유기물 등 다양한 환경요인이 자실체의 발생에 영향을 주는 것으로 알려져 있다. 본 연구에서는 기온변화 양상만을 분석한 결과, 흰가시광대버섯의 발생과 기온과의 관련성은 조사지역별로 다른 양상을 보였으며, 흰가시광대버섯의 재배생산기술을 개발하기 위해서는 식물상과 토양환경에 대한 정밀한 조사분석을 통해 자실체발생을 좌우하는 요인의 구명이 필요하다.

• 대한의용생체공학회:의공학회지
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• 제39권6호
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• pp.250-255
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• 2018

Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.

• Journal of Ecology and Environment
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• 제43권3호
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• pp.320-331
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• 2019

Background: Dioecious plant species having both male and female plants have been investigated regarding sex-related characteristics such as sex ratio, sex-differential resource requirements, and spatial segregation of the sexes. Habitat loss and fragmentation are major threats to the survival of plant populations, but dioecious species are particularly more prone to such habitat degradation than non-dioecious species because of their dimorphic sexual system. We examined the sex-related demographics of two Ilex cornuta populations being different regarding land use history. Methods: During 2016-2017, we examined I. cornuta trees with a basal diameter ${\geq}1.5cm$ in the Yongsu-ri population (YS population) and the Gotjawal Provincial Park population (GP population). Plant sex (male, female, or unsexed) was identified. The tree size (basal diameter and height of the main stem), clonal production (the ramet numbers per genet), and vitality for each clone were measured. The associations between population, sex, tree size, clonal production, and vitality were examined using ANOVAs and contingency table analyses. Finally, point pattern analyses using O-ring statistics were conducted to assess spatial patterns. Results: Upon excluding unsexed trees, the YS population with 74 trees was significantly male-biased (0.66), while the GP population with only 26 trees had a 1:1 sex ratio. In both populations, males and females did not differ in tree size. Although the mean number of ramets differed significantly between populations, females tended to produce more ramets than males. The proportion of weak trees was significantly higher in the YS than in the GP population. Neither population showed evidence of spatial segregation of the sexes. Conclusions: The two populations of dioecious I. cornuta are characterized by the small number of trees and relatively high frequencies of non-reproductive trees. Both indicate that these populations are quite susceptible to environmental and genetic stochasticity. On the other hand, the differences between populations in sex ratio, clonal production, and vitality suggest that conservation efforts for I. cornuta need to be population-specific. In order to help recover and enable this vulnerable species to persist, it is necessary to find ways to enhance their sexual reproduction and simultaneously reduce habitat disturbances due to anthropogenic activities.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.58-58
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• 2019

덕항산 산림유전자원보호구역(337ha)은 강원도 삼척시 신기면에 위치하며, 남한에서 유일하게 해발고도 1,000m에 가까운 고산지대에 발달한 카르스트지형을 가지고 있다. 본 연구는 석회암지대의 식생현황을 제공하여 석회석 광산 채굴 등 황폐화가 진행되고 있는 석회암지대의 식생을 복원하기위한 식생자료로 활용되고자 실시되었다. 2018년 4월 9일부터 10월 31일 까지 총 15회에 걸친 계절별 현지 조사를 실시한 결과, 덕항산 지역에서 발견된 관속식물은 90과 302속 479종 4아종 51변종 3품종 총 537분류군으로 조사되었다. 그 중 한국특산식물은 북부지방 고산지대에 자생한다고 알려진 Pseudostellaria setulosa Ohwi(숲개별꽃)과 우리나라 석회암지대에서만 발견된다고 알려진 Saussurea calcicola Nakai(사창분취)와 Peucedanum insolens Kitag.(덕우기름나물)을 포함하여 18분류군이 발견되었고, 희귀 및 멸종위기식물은 멸종위기 야생식물 II급에 속하는 Astilboides tabularis (Hmsl.) Engl.(개병풍)과 Vioela websteri Hemsl.(왕제비꽃)을 포함하여 18분류군이 분포하였다. 또한 석회암지대 지표식물 33분류군, 식물구계학적 특정식물 총 113분류군이 조사되었으며, 귀화식물은 27분류군이 확인되었다. 기후변화 취약식물은 특산식물 11분류군, 북방계식물 22분류군으로 총 33분류군이 조사되었다. 본 대상지는 온대북부 석회암지대의 특성상 특산식물과 북방계식물이 주로 발견되었으며, 남방계식물은 현재까지 발견되지 않았다. 지구온난화로 인한 남방계 식물의 북상은 우려와는 달리 현재까지는 미비한 수준으로 판단되며, 북방계식물의 생육에 적합한 환경을 유지하고 있는 것으로 판단된다.

• 동물자원연구
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• 제28권2호
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• pp.78-96
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• 2017

구제역은 발생 시 전염이 쉽게 일어나며 심각한 경제적 피해를 일으키는 질병이다. 구제역의 방역정책은 발견 직후 빠른 살처분이 최선책이나, 전파 속도나 상황 등에 따라 타지역 백신 접종 등의 방법을 시행할 수도 있다. 이러한 방법을 적용하기 위해서는 구제역을 빠르고 정확하게 진단할 필요성이 있다. 개발된 진단법들은 구제역의 확진, 혈청형의 동정, 백신 접종 후 항체의 생성 확인 등에 사용된다. 많은 진단법들이 개발되었지만 아직은 빠른 시간 내에 검출이 가능하며 동시에 정확성도 가진 방법이 드물다. 그렇기에 기존의 방법들을 개선시킨 새로운 진단법이 필요하다. 현재는 대부분 혈청학적 진단법인 ELISA에 의존하거나 분자 유전학적 기술인 PCR을 사용한다. 가장 최근 기술은 그 둘을 합치는 방법으로, 어떻게 하면 더 신속하고 저비용이면서, 민감하고 정확한 방법이 될 수 있을지 연구가 진행되고 있다.

• 한국약용작물학회지
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• 제28권6호
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• pp.445-454
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• 2020

Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.

• Journal of Animal Science and Technology
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• 제63권2호
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• pp.262-271
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• 2021

Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice, rats, bats, and pigs; however, these two markers have not yet been described in the epididymis of bulls. Here, we examined the presence and distribution of the B1 subunit of V-ATPase (B1-VATPase) and KRT5 in the distinct regions of adult bovine epididymides, specifically, the caput, corpus, and cauda. Immunofluorescence staining and confocal microscopy showed that narrow shaped-clear cells were placed in the caput and corpus regions of the bovine epididymis; however, they were absent in the cauda epididymis. In addition, B1-VATPase was highly expressed in the cauda spermatozoa; however, it was rarely detected in the caput spermatozoa. On the other hand, KRT5-positive cells, basal cells, were maintained beneath the basal lamina and they had the traditional form with a dome-shaped morphology from the caput to cauda region of the bovine epididymis. The co-expression of B1-VATPase and KRT5 was confined to basal cells placed in the basal region of the epithelium. In summary, 1) clear cells were present with region-specific localization, 2) B1-VATPase was present in the corpus and cauda spermatozoa but absent in the caput, 3) co-expressed cells with B1-VATPase and KRT5 were present in the adult bovine epididymis, and 4) B1-VATPase was not a specific marker for clear cells in the bovine epididymis. Therefore, the perfect epididymal luminal condition created by the specific expression and localization patterns of B1-VATPase might be necessary to obtain fertilizing capacity of spermatozoa in the bovine epididymis.