• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.274-281
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• 2019

Pistacia chinensis Bunge has not only been used as a medicinal plant to treat various illnesses but its young shoots and leaves have also been used as vegetables. In addition, P. chinensis is used as a rootstock for Pistacia vera (pistachio). Here, the transcriptome of P. chinensis was sequenced to enrich genetic resources and identify secondary metabolite biosynthetic pathways using Illumina RNA-seq methods. De novo assembly resulted in 18,524 unigenes with an average length of 873 bp from 19 million RNA-seq reads. A Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation tool assigned KO (KEGG orthology) numbers to 6,553 (36.2%) unigenes, among which 4,061 unigenes were mapped into 391 different metabolic pathways. For terpenoid backbone and carotenoid biosynthesis pathways, 44 and 22 unigenes encode enzymes corresponding to 30 and 16 entries, respectively. Twenty-two unigenes encode proteins for 16 entries of the carotenoid biosynthesis pathway. As for the phenylpropanoid and flavonoid biosynthesis pathways, 63 and 24 unigenes were homologous to 17 and 14 entry proteins, respectively. Mining of simple sequence repeat identified 2,599 simple sequence repeats from P. chinensis unigenes. The results of the present study provide a valuable resource for in-depth studies on comparative and functional genomics to unravel the underlying mechanisms of the medicinal properties of Pistacia L.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.3
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• pp.325-333
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• 1994

This study was undertaken at Gowa, South Sulawesi, Indonesia as part of a larger forage genetic resource evaluation project. The experimental program consisted of a field experiment where grasses and legume species were grown in monocultures and the yield, N content and rumen bag digestibility using goats were measured. The field experiment was conducted from December 1985 to October 1986. Eight species of legumes (Desmodium heterophyllum cv. John-stone, Desmodium triflorum from Gowa, South Sulawesi, Arachis sp. from Maiwa, South Sulawesi, Clitoria ternalea CPI 50973, Macroptilium atropurpureum ev. Siratro, Neonotonia wightii cv. Tinaroo, Centrosema pubescens CPI 58575, Centrosema plumeri CPI 58568) were grown as monocultures. After establishment all plants were cut to a uniform height of 5 cm, and subsequent cuts were made on regrowth after 14, 28, 42, and 56 days (cycle 1). Cycle 2 commenced towards the end of the wet season and continued for 157 days into the dry season. The highest yielding legumes were C. ternatea in the wet season and Arachis sp. in the dry season. The mean rumen bag dry matter digestibility (RBDMD) of legumes of 67.6% for leaf material (averaged over all cycles and ages) was 7.6% higher than for stem material. The RBDMD of Arachis was significantly higher than all other species. The RBDMD of all legumes declined with age. Calculation of yield of digestible DM (yield $\times$ RBDMD) showed that Arachis sp. was the best legume. The combination of plant "quality" with yield measures is a valuable adjunct to routine agronomic survey procedures in plant evaluation programs. Arachis sp. appears to offer considerable promise and should be more widely evaluated.

• Research in Plant Disease
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• v.19 no.1
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• pp.31-35
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• 2013

Thirteen soybean cyst nematode (SCN) (Heterodera glycines) populations collected in Korea were examined in their HG type by their reproductivity on 7 Plant Introduction indicators for the identification of HG type. Six HG types were identified, HG type 0, 2, 5, 2.5, 1.2.7, and 2.5.7. HG type 2.5 was the most frequent (4 samples, 30.8%), followed by HG type 2.5.7 (3 samples, 23.0%). About 76.9% of SCN populations were reproduced on PI 88788, followed by PI 209332 (61.5%), PI 548316 ('Cloud') (30.8%), and PI 548402 ('Peking') (7.7%). No population could reproduce on PI 90763, PI 437654, thus, they could be used for resistant source for developing SCN resistant soybean in Korea.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.293-301
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• 2016

High-quality gene sets are necessary for functional research of genes. Although Perilla is a commonly cultivated oil crop and vegetable crop in Southeast Asia, the quality of its available gene set is insufficient. To construct a high-quality Perilla gene set, we sequenced mRNAs extracted from different tissues of Perilla citriodora, the wild species (2n = 20) of Perilla. To make a high-quality gene set for P. citriodora, we compared the quality of assemblies produced by Velvet and Trinity, the two well-known de novo assemblers, and improved the de novo assembly pipeline by optimizing k-mers and removing redundant sequences. We then selected representative transcripts for loci according to several criteria. The improved assembly yielded a total of 86,396 transcripts and 38,413 representative transcripts. We evaluated the assembled transcripts by comparing them to 638 homologous Arabidopsis genes involved in fatty acid and TAG biosynthesis pathways. High proportions of full-length genes and transcripts in the assembled transcripts matched known genes in other species, indicating that the P. citriodora gene set can be applied in future functional studies. Our study provides a reference P. citriodora gene set for further studies. It will serve as valuable genetic resource to elucidate the molecular basis of various metabolisms.

• Korean Journal of Poultry Science
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• v.39 no.2
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• pp.105-112
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• 2012

Genetic resource conservation of indigenous species is important to conserve terrestrial, aquatic and avian species throughout the world. The native Korean Oge (KO) chickens are important and protected indigenous avian species in Korea. This KO breed is very popular in Korea because of its external appearances of blackish color. The characterization of morphology and growth performances of KO were performed since 1980s, however the information is still not sufficient for breed selection. In this study, we compared various growth performances including body growth, tibia length, shank length, chest width, chest grith and length of tail feather between male and female chickens of KO and widely known White Leghorn (WL) breeds at 1 week, 2 weeks, 5 weeks, 10 weeks, and 24 weeks. We observed differences on various growth performances at different aged groups between KO and WL chickens. This study may help for the selection of chicken breeds based on age, body growth and meat production.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.11a
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• pp.145-167
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• 1999

Our self-sufficiency of food has become less than $30{\%}$ and our nation is highly dependant on world's grain market for food. which is unstable in long term due to the world population growth faster than food production. Therefore, it is a great possibility that food might become a political weapon by way of its global shortage. its purchasing difficulty in international free trade market. and the resultant price rising. Our maximal capability of food production has become the most outstanding problem in the dimension of future food security. It would be the utmost scheme for maximal production of food to realize the maximal utilization of arable land through the enlargement of sufficient farming land and the conversion of rotation system for the more grain production. Extensional enlargement of arable land can be positively executed through the development of farming land in domestic and abroad countries. The readjustment of arable land and the installation or irrigation and drainage system can enforce the farming basement for maximal utilization of arable land through the improved rotation between paddy and upland. The prevention policy against farming land encroachment should be strictly executed through grain production encouragement on resting or marginal lands and regulation of utilization conversion for the other than food production on high grade farming lands. It is also required urgently to develope high yielding and high quality varieties through advanced genetic technology for the improvement of unit area yield, especially of wheat, corn. and soybean we import in large quantity The maximal utilization of arable land for the highest production of food can be realized through rational rotation system, the most adaptable crop cultivation on the suitable land, and the most optimal fertilization through the GIS analysis of agricultural environment information on the basis of the computerized soil resource data on super detailed soil maps(1:5000) surveyed plot by plot of whole nation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.49-49
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• 2018

본 연구는 함백산 산림유전자원보호구역의 생물다양성 증진과 체계적이고 객관적인 보전 및 관리를 위한 기초데이타 수립을 위하여 식물상과 정밀식생조사를 수행하였다. 함백산 산림유존자원보호구역에 분포하는 식물상은 83과 222속 321종 2아종 36변종 등 359분류군이 확인되었으며, 희귀 및 멸종위기식물은 산마늘, 등칡 등 총 10과 14분류군이 확인되었다. 식물구계학적 3등급 종은 15과 20분류군, 4등 급종은 8과 11분류군이 확인되었다. 산림청 특별산림보호 대상종은 3과 3분류군, 한국특산식물은 7과 10분류군이 확인되었으며 귀화식물은 9과 16분류군이 출현하였다. 함백산 산림유전자원보호구역 26개 조사구역에 대하여 정밀식생조사를 수행한 결과. 식생유형은 산지관목림, 일본잎갈나무식재림, 가래나무군락, 거제수나무군락, 거제수나무-신갈나무군락, 거제수나무-층층나무군락, 거제수나무-피나무군락, 소나무군락, 신갈나무군락, 피나무군락, 피나무-거제수나무군락 등 총 11개의 유형으로 확인되었다. 또한 식생조사와 드론을 활용하여 현존식생도를 작성하였으며, 그 결과 함백산 유전자원보호구역은 총 470.9ha로, 그 중 신갈나무군락이 총 390.8ha로 가장 넓게 분포하고 있으며, 거제수나무-신갈나무군락이 34.3ha, 산지관목림 18.5ha 순으로 대부분 10ha 이하로 나타났다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.46-46
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• 2018

본 연구는 봉화군 청옥산 산림유전자원보호구역에 분포하는 관속식물을 파악하고자 수행하였다. 청옥산 보호구역은 산림청에서 '희귀식물자생지' 유형으로 지정하여 관리하고 있으며, 면적은 약 2,051ha에 달하며 주로 신갈나무군락과 소나무군락이 우점하고있다. 현지조사는 2017년 3월부터 10월까지 계절별로 수행하였다. 조사결과, 보호구역내 관속식물은 86과 258속 390종 4아종 54변종 6품종으로 총 454분류군의 분포가 확인되었다. 이 중 한국특산식물은 가야물봉선, 처녀치마, 할미밀망, 키버들 등 11분류군이며, 산림청 지정 희귀식물은 취약종(VU)에 주목, 백작약, 꼬리진달래 3분류군, 약관심종(LC)에 등칡, 도깨비부채, 정향나무, 말나리, 나도개감채 등 14분류군이다. 식물구계학적 특정식물로는 IV등급인 회리바람꽃, 가지괭이눈, 산겨릅나무, 귀박쥐나물 등 10분류군, III등급인 애기감둥사초, 중나리, 토현삼, 선갈퀴, 금강제비꽃, 노랑갈퀴, 분비나무 등 23분류군, II등급 32분류군, I등급 30분류군이 확인되었다. 외래식물은 오리새, 털별꽃아재비, 왕포아풀, 주걱개망초, 지느러미엉겅퀴, 족제비싸리 등 18분류군이 임도 주변에서 확인되었다. 조사된 관속식물을 용도별로 구분하면 식용식물 181분류군, 섬유용식물 6분류군, 약용식물 143분류군, 관상용식물 52분류군, 사료용식물 172분류군, 염료용식물 9분류군, 목재용식물 22분류군으로 분석되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.47-47
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• 2018

본 연구는 청옥산 산림유전자원보호구역을 대상으로 총 49개의 고정방형구를 설치하여 식물사회학적 방법으로 산림군락분류와 임분특성을 분석하였다. 그 결과 총 9개의 식생단위로 분류되었으며 군락단위로는 신갈나무군락, 잣나무군락, 일본잎갈나무군락, 독일가문비나무군락으로 분류되었다. 신갈나무군락은 소나무군, 철쭉군, 고로쇠나무군, 미역줄나무군으로 세분되었으며, 고로쇠나무군은 박달나무소군과 고로쇠나무전형소군으로, 미역줄나무군은 애기감둥사초소군과 미역줄나무전형군으로 세분되었다. 9개의 식생단위의 층위별 중요치 분석결과를 보면 교목층에서 신갈나무가 32.2% 가장 높게 나타났으며 잣나무(10.1%), 소나무(10%), 일본잎갈나무(9.1%) 순으로 나타났고, 아교목층에서는 신갈나무 (18.9%), 당단풍나무(11.7%), 물푸레나무(8.9%), 잣나무(7.2%) 등이 높은 값으로 분석되었다. 관목층에서는 철쭉(12.2%)와 당단풍나무(8%)가 높게 분석되었으며 다른 수종들은 10% 미만의 값을 가졌다. 초본층에서는 조릿대(8.5%), 산숙국(6.6%)등이 높게 나타났다. 식생단위별 종다양성지수를 보면 식생단위 8인 일본잎갈나무군락이 1.76로 가장 높았으며, 박달나무소군 1.73, 소나무군 1.72, 철쭉군 1.71 순으로 분석되었다. 우점도는 모든 식생단위에서 0.2 이하로 다수의 종이 우점하는 것으로 나타났다.

• Genomics & Informatics
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• v.12 no.4
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• pp.181-186
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• 2014

Genome-wide association studies have proven the highly polygenic architecture of complex diseases or traits; therefore, single-locus-based methods are usually unable to detect all involved loci, especially when individual loci exert small effects. Moreover, the majority of associated single-nucleotide polymorphisms resides in non-coding regions, making it difficult to understand their phenotypic contribution. In this work, we studied epistatic interactions associated with three common diseases using Korea Association Resource (KARE) data: type 2 diabetes mellitus (DM), hypertension (HT), and coronary artery disease (CAD). We showed that epistatic single-nucleotide polymorphisms (SNPs) were enriched in enhancers, as well as in DNase I footprints (the Encyclopedia of DNA Elements [ENCODE] Project Consortium 2012), which suggested that the disruption of the regulatory regions where transcription factors bind may be involved in the disease mechanism. Accordingly, to identify the genes affected by the SNPs, we employed whole-genome multiple-cell-type enhancer data which discovered using DNase I profiles and Cap Analysis Gene Expression (CAGE). Assigned genes were significantly enriched in known disease associated gene sets, which were explored based on the literature, suggesting that this approach is useful for detecting relevant affected genes. In our knowledge-based epistatic network, the three diseases share many associated genes and are also closely related with each other through many epistatic interactions. These findings elucidate the genetic basis of the close relationship between DM, HT, and CAD.