• 한국산림과학회지
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• 제109권2호
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• pp.189-194
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• 2020

두메닥나무 (Daphne pseudomezereum var. koreana)는 강원도, 전라북도, 경상도의 고산지대에 분포하는 한국 자생종이다. 최근 자생지가 축소되어 개체 수가 급격히 줄어들고 있어 멸종위기종으로 지정되었다. 두메닥나무의 추출물은 화장품 등에 유용한 소재로 이용되나 식물체의 번식 효율은 매우 낮은 실정이다. 본 연구는 두메닥나무의 유전자원 보존과 생물소재로 활용을 위해 대량증식법을 확립하고자 하였다. 온실에서 자란 두메닥나무의 액아를 포함한 줄기절편을 생장이 우수했던 WPM 배지를 기본 조성으로 하여 BA와 GA₃를 혼용 처리한 4가지 배지에서 기내줄기의 정단의 유무에 따른 줄기생장과 신초유도를 비교하였다. 두메닥나무는 정아가 포함된 절편체는 BA 8μM + GA₃8μM 조성에서 줄기생장이 가장 우수하였으며, 정아가 존재하지 않을 때 BA 8μM + GA₃8μM 조성에서 신초유도가 가장 우수하였다. 반면 발근유도는 NAA 1μM, NAA 5μM, NAA 10μM 조성 모두 발근이 발생하지 않은 대신 잎과 줄기가 생장하여 효과가 없는 것으로 나타났다.

• 환경정책연구
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• 제1권1호
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• pp.25-45
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• 2002

Opening of trade relationships through an increasing number of international free trade agreements and the now defunct General Agreement on Tariffs and Trade has resulted in an increase the number of the species being exchanged in the world. In the last 20 years, international environmental laws have multiplied and a number of treaties address harmful non-indigenous species (NIS) directly with specific provisions, while other treaties deal with related environmental issues and indirectly affect international regulation of NIS; however, such treaties are weak due to lack of enforceability. From the stand point of national law, many countries including the USA, Australia and New Zealand enforce national laws and regulations to protect biological resources. Typical strategies include : 1) strengthening quarantines to prevent unintentional and illegal introduction of harmful NIS, and 2) developing technologies for managing harmful NIS. However, the recent international trend for managing NIS has shifted. In 2002, the Bonn Guidelines on Access to Genetic Resources and Fair and Equitable Sharing of the Benefit Arising out of their Utilization was adopted at the 6th Conference of the Parties to the Convention on Biodiversity. One major issue highlighted in the document is that "there is no more free of charge to get a biological resource from other countries". The Bonn guidelines will affect international and national NIS regulatory systems because the NIS is a potentially disrupts ecosystems as well as native species. A number of impacts are expected including the revamping of national biodiversity policy regimes in many countries in the world. In particular, the ROK, which is not very biologically diverse, has to evolve national laws to protect valuable ecosystems from NIS. In the meanwhile, national rights of using beneficial indigenous and non-indigenous species as biological resources should be considered through the investigation and national registration of NIS around the world for the promotion of the biotech industry.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.563-570
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• 2016

S. griseus wild type에서 dasA 유전자의 과발현에 의해 유도된 기저균사의 ectopic sporulation 관련 유전자를 알아보기 위해서, empty vector가 삽입된 균주와 dasA가 과발현된 균주의 전사체를 DNA microarray법으로 비교하였다. DNA microarray 결과를 토대로 dasA 유전자 과발현 균주에서 2배이상 발현량이 증가되었으며 p-value가 0.05 미만(p-value < 0.05)인 유전자들 중에서 false positive 를 제외시키는 작업을 통하여 최종적으로 4개의 유전자(SGR794, SGR2469, SGR3656, SGR3657)와 3개의 cluster (SGR795-797, SGR2377-2378, SGR6997-6998)를 선발하였다. 이들의 전사량은 low resolution Sl nuclease mapping 법을 통하여 dasA 유전자 과발현 균주에서 증가된 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권9호
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• pp.1354-1361
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• 2015

The complement system is a part of the natural immune regulation mechanism against invading pathogens. Complement activation from three different pathways (classical, lectin, and alternative) leads to the formation of C5-convertase, an enzyme for cleavage of C5 into C5a and C5b, followed by C6, C7, C8, and C9 in membrane attack complex. The C9 is the last complement component of the terminal lytic pathway, which plays an important role in lysis of the target cells depending on its self-polymerization to form transmembrane channels. To address the association of C9 with traits related to disease resistance, the complete porcine C9 cDNA was comparatively sequenced to detect single nucleotide polymorphisms (SNPs) in pigs of the breeds Hampshire (HS), Duroc (DU), Berlin miniature pig (BMP), German Landrace (LR), Pietrain (PIE), and Muong Khuong (Vietnamese potbelly pig). Genotyping was performed in 417 $F_2$ animals of a resource population (DUMI: $DU{\times}BMP$) that were vaccinated with Mycoplasma hyopneumoniae, Aujeszky diseases virus and porcine respiratory and reproductive syndrome virus at 6, 14 and 16 weeks of age, respectively. Two SNPs were detected within the third exon. One of them has an amino acid substitution. The European porcine breeds (LR and PIE) show higher allele frequency of these SNPs than Vietnamese porcine breed (MK). Association of the substitution SNP with hemolytic complement activity indicated statistically significant differences between genotypes in the classical pathway but not in the alternative pathway. The interactions between eight time points of measurement of complement activity before and after vaccinations and genotypes were significantly different. The difference in hemolytic complement activity in the both pathways depends on genotype, kind of vaccine, age and the interaction to the other complement components. These results promote the porcine C9 (pC9) as a candidate gene to improve general animal health in the future.

• 한국콘텐츠학회논문지
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• 제7권9호
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• pp.176-182
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• 2007

천연기념물은 자연의 산물이며 인류가 보호해야 할 유산이다. 천연기념물 중 동물과 식물은 생명을 가지고 있기 때문에 하나의 개체는 반드시 소멸한다. 천연기념물인 보은 속리산의 정이품송은 유일하다. 현재 정이품송은 자연재해와 병충해로 인해 곤란을 겪고 있으며 수령이 600년 이상이 되어 고사의 위기에 처해있다. 본 연구의 목적은 국가적으로 유지하여야 하는 희귀식물의 혈통을 보존하고 매력 있는 식물자원의 상품, 지역주민의 대량증식에 의한 소득창출 등을 할 수 있는 대상으로서 천연기념물인 정이품송의 관광자원화 방안을 모색하고자 하는 것이다. 연구의 목적을 달성하기 위한 방안으로서 식물유전자분석기술을 활용한 정이품송 혈통인증, 순종보존과 스토리텔링과 접목한 족보관리시스템 운용, 생산 판매 소유자 간 커뮤니티 제공과 데이터베이스 구축, 문화관광이벤트의 개최, 정이품송의 가치 보존과 브랜드화 등을 제시하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권1호
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• pp.116-123
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• 2003

Forages are the most important feed resource for ruminants worldwide, whether fed as pastures, forage crops or conserved hay, silage or haylage. There is large variability in the quality of forages so measurement and prediction of feeding value and nutritive value are essential for high levels of production. Within a commercial animal production system, methods of prediction must be inexpensive and rapid. At least 50% of the variation in feeding value of forages is due to variation in voluntary feed intake. Identification of the factors that constrain voluntary feed intake allows these differences to be managed and exploited in forage selection. Constraints to intake have been predicted using combinations of metabolic and physical factors within the animal while simple measurements such as the energy required to shear the plant material are related to constraints to intake with some plant material. Animals respond to both pre- and post-ingestive feedback signals from forages. Pre-ingestive signals may play a role in intake with signals including taste, odour and texture together with learned aversions to nutrients or toxins (post-ingestive feedback signals). The challenge to forage evaluation is identification of the factors which are most important contributors to these feedback signals. Empirical models incorporating chemical composition are also widely used. The models tend to be useful within the ranges of the datasets used in their development but none can claim to have universal application. Mechanistic models are becoming increasingly complex and sophisticated and incorporate both feed characteristics and use of biochemical pathways within the animal. Improvement in utilisation through the deliberate selection of pasture plants for high feeding value appears to have potential and has been poorly exploited. Use of Near Infrared Reflectance Spectroscopy is a simple method that offers significant potential for the preliminary screening of plants with genetic differences in feeding value. Near Infrared Reflectance Spectroscopy will only be as reliable as the calibration sets from which the equations are generated.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1379-1385
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• 2017

The content of taxol in the bark of yews is very low, and this is not affordable from the environmental point of view. Thus, it is a necessity to look for alternative sources of taxol production to solve its supply. Currently, a large portion of the taxol in the market comes from chemical semi-synthesis, but the semi-synthetic precursors such as baccatin III and 10-deacetyl-baccatin III are extracted from needles and twigs of yew trees. Taxol-producing fungi as a renewable resource is a very promising way to increase the scale of taxol production. Our group has obtained a taxol-producing endophytic fungus, Aspergillus niger subsp. taxi HD86-9, to examine if A. niger can produce the taxanes. Six compounds from the fermentation broth of strain HD86-9 were isolated and identified by $^1H$ NMR, $^{13}C$ NMR, and ESI-MS. The results showed that the six compounds included four taxane diterpenoids (taxol, cephalomannine, baccatin III, and 10-deacetyl-baccatin III) and two non-taxane compounds (${\beta}-sitosterol$ and flavonoid isovitexin). The study verified that the taxanes can be produced by the A. niger, which is very important to taxol production via chemical semi-synthesis. Additionally, the finding is potentially very significant to solve the taxol semi-synthetic precursors extracted from needles and twigs of yew trees, and the precursor production can be easily increased through the culture condition optimization, genetic breeding, and metabolic engineering of the A. niger.

• 아시안잔디학회지
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• 제25권2호
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• pp.237-242
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• 2011

2011년 일본잔디학회 추계학술대회는 크게 일반 학술강연, 부회 및 소심포지움으로 나뉘어 총 55주제가 발표되었다. 일반 학술강연은 잔디, 병해충 및 잡초의 생리 생태분야, 병해 및 잡초방제분야 및 신규 잔디용 약제에 대한 약효특성 연구결과에 대해 발표되었다. 그중 잔디밭 최신 발생병해인 necrotic ring spot과 문제잡초인 파대가리, 새포아풀, 점나도나물에 대한 방제방안 및 UV를 이용한 살초스펙트럼을 조사한 연구발표가 있었다. 또한, 신규 등록 살균제 Furametpyr와 제초제인 S-metolachlor, Flucetosulfuron 및 Foramsulfuron의 방제효과에 대한 연구결과가 발표되었다. 부회에서는 골프장, 교정잔디, 공원녹지 및 지피식물 녹화의 4가지 주제와 잔디 유전자원 평가라는 소심포지움을 통해 다양한 정보교류가 있었다. 특히, 공원녹지 부회에서는 현재 일본의 사회적으로 가장 큰 이슈인 방사능오염물질 누출에 의한 공원, 교정 및 가정의 잔디밭의 방사능물질 오염 메카니즘과 오염물질을 효과적으로 제거하는 방법에 대하여 발표되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.451-455
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• 2015

The buffalo is an important livestock resource in several countries of South Asia and the Mediterranean regions. However, reproductive efficiency is compromised due to known problems of biological and management origins, such as lack of animal selection and poor nutrition. Under optimal conditions puberty is attained at 15 to 18 months in river buffalo, 21 to 24 months in swamp buffalo and is influenced by genotype, nutrition, management and climate. However, under field conditions these values deteriorate up to a significant extant. To improve reproductive efficiency, several protocols of oestrus and ovulation synchronization have been adopted from their use in commercial cattle production. These protocols yield encouraging pregnancy rates of (30% to 50%), which are comparable to those achieved in buffaloes bred at natural oestrus. The use of sexed semen in buffalo heifers also showed promising pregnancy rates (50%) when compared with conventional non-sexed semen. Assisted reproductive technologies have been transferred and adapted to buffalo but the efficiency of these technologies are low. However, these latest technologies offer the opportunity to accelerate the genetic gain in the buffalo industry after improving the technology and reducing its cost. Most buffaloes are kept under the small holder farming system in developing countries. Hence, future research should focus on simple, adoptable and impact-oriented approaches which identify the factors determining low fertility and oestrus behaviour in this species. Furthermore, role of kisspeptin needs to be explored in buffalo.

• 한국환경복원기술학회지
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• 제21권2호
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• pp.27-38
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• 2018

Environmental planning includes resource allocation and spatial planning process for the conservation and management of environment. Because the spatialization of the environmental planning is not specifically addressed in the relevant statutes, it actually depends on the qualitative methodology such as expert judgement. The results of the qualitative methodology have the advantage that the accumulated knowledge and intuition of the experts can be utilized. However, it is difficult to objectively judge whether it is enough to solve the original problem or whether it is the best of the possible scenarios. Therefore, this study proposed a methodology to quantitatively and objectively spatialize various environmental planning. At first, we suggested a quantitative spatial planning model based on an optimization algorithm. Secondly, we applied this model to two kinds of environmental planning and discussed about the model performance to present the applicability. Since the models were developed based on conceptual study site, there was a limitation in showing possibility of practical use. However, we expected that this study can contribute to the fields related to environmental planning by suggesting flexible and novel methodology.