• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.35-35
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• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• Wind and Structures
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• 제39권1호
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• pp.47-69
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• 2024

Pounding of structures may result in considerable damages, to the extent of total failure during severe lateral loading events (e.g., earthquakes and wind). With the new generation of tall buildings in densely occupied locations, wind-induced pounding becomes of higher risk due to such structures' large deflections. This paper aims to develop mathematical formulations to determine the maximum pounding force when two adjacent structures come into contact. The study will first investigate wind-induced pounding forces of two equal-height structures with similar dynamic properties. The wind loads will be extracted from the Large Eddy Simulation models and applied to a Finite Element Method model to determine deflections and pounding forces. A Genetic Algorithm is lastly utilized to optimize fitting parameters used to correlate the maximum pounding force to the governing structural parameters. The results of the wind-induced pounding show that structures with a higher natural frequency will produce lower maximum pounding forces than those of the same structure with a lower natural frequency. In addition, taller structures are more susceptible to stronger pounding forces at closer separation distances. It was also found that the complexity of the mathematical formula from optimization depends on achieving a more accurate mapping for the trained database.

• 대한의용생체공학회:의공학회지
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• 제45권4호
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• pp.195-203
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• 2024

Muscle disorders arise from genetic factors or other external environmental influences, leading to muscle loss and decreased strength. Muscle disease is closely related to age, and with the global progression of aging, there is increasing interest in the prevention and treatment of muscle diseases among the elderly population. Muscles release various substances during contraction and relaxation when exercising, and growth and differentiation are enhanced. Similar effects can be achieved by vibration stimulations of specific frequencies. To examine the response of muscle cells to vibration stimuli, an in vitro vibration device capable of setting various frequencies and amplitudes was developed for use with a 6-well plate. In vitro vibrational stimulation was applied to C2C12 myoblasts to quantify the growth and differentiation of myoblasts, as well as cell apoptosis. 10 Hz vibrational stimuli resulted in increases in ERK signaling and decreases in cell death. Moreover, an increase in the number of exposures to vibration promoted cellular differentiation. In conclusion, 10 Hz vibrational stimuli have the potential to increase muscle growth and differentiation and reduce apoptosis in C2C12 myoblasts.

• Steel and Composite Structures
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• 제34권5호
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• pp.743-767
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• 2020

Due to the impressive flexural performance, enhanced compressive strength and more constrained crack propagation, Fibre-reinforced concrete (FRC) have been widely employed in the construction application. Majority of experimental studies have focused on the seismic behavior of FRC columns. Based on the valid experimental data obtained from the previous studies, the current study has evaluated the seismic response and compressive strength of FRC rectangular columns while following hybrid metaheuristic techniques. Due to the non-linearity of seismic data, Adaptive neuro-fuzzy inference system (ANFIS) has been incorporated with metaheuristic algorithms. 317 different datasets from FRC column tests has been applied as one database in order to determine the most influential factor on the ultimate strengths of FRC rectangular columns subjected to the simulated seismic loading. ANFIS has been used with the incorporation of Particle Swarm Optimization (PSO) and Genetic algorithm (GA). For the analysis of the attained results, Extreme learning machine (ELM) as an authentic prediction method has been concurrently used. The variable selection procedure is to choose the most dominant parameters affecting the ultimate strengths of FRC rectangular columns subjected to simulated seismic loading. Accordingly, the results have shown that ANFIS-PSO has successfully predicted the seismic lateral load with R² = 0.857 and 0.902 for the test and train phase, respectively, nominated as the lateral load prediction estimator. On the other hand, in case of compressive strength prediction, ELM is to predict the compressive strength with R² = 0.657 and 0.862 for test and train phase, respectively. The results have shown that the seismic lateral force trend is more predictable than the compressive strength of FRC rectangular columns, in which the best results belong to the lateral force prediction. Compressive strength prediction has illustrated a significant deviation above 40 Mpa which could be related to the considerable non-linearity and possible empirical shortcomings. Finally, employing ANFIS-GA and ANFIS-PSO techniques to evaluate the seismic response of FRC are a promising reliable approach to be replaced for high cost and time-consuming experimental tests.

• 한국산림과학회지
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• 제101권4호
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• pp.619-625
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• 2012

본 연구는 국내 분포하는 가침박달 집단의 열매, 종자 및 발아 특성에 대한 변이를 조사하였다. 5개 자연집단의 96개 개체목에서 열매를 채취하여 5가지 열매특성, 7가지 종자특성 및 3가지 발아특성을 분석하였다. 평균발아 일수를 제외한 14가지 특성들에서 집단간 및 집단내 개체간에 유의적인 차이가 인정되었다. 열매와 종자 특성은 대체적으로 전체 분산 가운데 집단이 차지하는 비율이 높은 것으로 나타났다. 변이계수 값을 보면 열매특성은 열매무게 27.0%, 종자특성은 종자길이/두께 28.1%, 발아특성은 발아속도 52.5%로 가장 높은 값을 나타냈다. 가침박달 집단의 평균 발아특성은 발아율 62.9%, 평균발아일수 64.0일, 발아속도 0.40개/일로 나타났다. 상관분석결과 평균발아일수는 종자두께와 발아속도는 모수의 수고 및 위도와 각각 정의 상관관계를 나타냈다. 또한 위도가 높을수록 열매가 무겁게 나타나는 정의 상관관계를 나타냈다. 군집분석결과 집단의 분포에 따른 지리적인 경향은 나타나지 않았다. 주성분분석 결과 제1주성분의 기여도가 전체변이의 63%를 설명하였으며, 고유값(Eigenvalue)이 1 이상인 제3주성분까지의 기여도는 97%로 가침박달 열매, 종자 및 발아특성 분류에 중요한 정보를 주는 요인으로 나타났다.

• 한국강구조학회 논문집
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• 제19권1호
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• pp.15-27
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• 2007

지난 10년간 건설 환경에서 노무비는 재료비에 대해 상대적으로 계속해서 증가하고 있다. 따라서 강구조물의 최적 설계를 위해 널리 쓰이고 있는 최소중량설계는 더 이상 최소경비설계를 의미하지 못한다. 최근의 강 구조물의 제작 시 재료비 외에 부재연결에 소요되는 경비가 실제로 총 제작비에 커다란 영향을 미치는 것을 알 수 있다. 그럼에도 불구하고 대부분의 연구가 최소 중량설계나 구조물의 고등해석에만 이루어지고 있다. 따라서 본 연구는 횡 방향 하중에 효과적으로 저항하도록 보와 기둥이 모멘트 연결된 강 뼈대 구조물을 대상으로 안정성에 문제가 없이 경비절감에 효과적인 설계를 위해 재료비뿐만 아니라 제작비 및 현장에서 가설 시 부재연결에 소요되는 경비를 포함하는 최소 경비 설계를 수행하고자 한다. 하중저항계수설계법에 따른 전단과 처짐 및 보-기둥 상관관계식을 포함하는 비선형 해석과정과 유전알고리즘을 바탕으로 한 최적화 알고리즘을 결합하여 모멘트 부재연결의 수를 줄이고 또한 효과적인 배치를 수행함으로 최적 설계 해 및 모멘트 연결의 수와 총 경비와의 상반관계를 나타내고 이로부터 최적의 모멘트 연결의 수 및 그 배치를 구하였다. 현실적인 하중조건을 고려한 수치 예를 통해 본 연구의 적용성과 효율성을 나타내었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• 한국전산구조공학회논문집
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• 제34권4호
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• pp.205-212
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• 2021

가거초 해양과학기지 자켓 구조물 내 콘크리트를 배제하고 강재로만 이루어진 최적설계를 제시한다. 50년 재현주기 극한하중조건에서 허용응력 및 허용응력비 조건을 모두 만족하는 안전한 경량 설계를 목표하였다. 역할에 따라 부재를 세 그룹으로 나눈 설계 조건(Case-1)과 보다 세분화한 설계 조건(Case-2)에 대해 각 부재그룹별 현재 단면 두께 대비 두께 변화율을 설계변수로 설정한 유전 알고리즘을 통해 최적설계를 탐색하였다. 그 결과 Case-1의 결과로 현재 가거초 해양과학기지보다 약 217톤 더 가벼운 설계(OPT-1)를 찾았고, Case-2에서는 추가적으로 약 84톤을 경량화하여 현재 대비 약 45%의 무게를 절감한 설계(OPT-2)를 얻을 수 있었다. 결론적으로 레그 내 콘크리트 보강 없이도 극한조건에서 허용응력 및 허용응력비를 모두 만족시킬 수 있는 경량화된 가거초 해양과학기지 설계를 제시하였다.

• 한국자원식물학회지
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• 제32권6호
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• pp.689-697
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• 2019

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. 'Wonkyo3114' and 'Gurumi40'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 cm × 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH₄NO₃-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0 mg/L GA₃, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA₃ for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

• 한국물환경학회지
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• 제29권2호
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• pp.184-195
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• 2013

Urbanization from agricultural/forest areas has been causing increased runoff and pollutant loads from it. Thus, numerous models have been developed to estimate NPS loading from urban area and Long-Term Hydrologic Impact Analysis (L-THIA) model has been used to evaluate effects of landuse changes on runoff and pollutant loads. However, the L-THIA model could not consider rainfall intensity in runoff evaluation. Therefore, the L-THIA model, capable of simulating runoff using 10-minute rainfall data, was applied to the study areas for evaluation of estimated runoff and NPS. The estimated Nash-Sutcliffe coefficient (NSE) values were over 0.6 for runoff, BOD, TN, and TP for most sites and watershed. The calibrated model was further extended to other counties for pollutant load potential evaluation. Pollutant load potential maps were developed and target areas were identified. As shown in this study, the L-THIA 2012 can be used for evaluation runoff and pollutant loads with limited data sets and its estimation could be used in identifying pollutant load hot spot areas for implementation of site-specific Best Management Practices.