• Journal of Veterinary Science
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• v.25 no.4
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• pp.55.1-55.12
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• 2024

Importance: Neonatal calf diarrhea is a major cause of mortality in newborn calves worldwide, posing a significant challenge in bovine herds. Group A Bovine Rotaviruses (BRVA) are the primary contributors to severe gastroenteritis in calves under two months old. Objectives: This study examined the prevalence and molecular characterization of BRVA in neonatal calves in Gujarat, India. Methods: Sixty-nine diarrheic fecal samples were collected and subjected to various molecular methods of BRVA detection, isolation, and characterization. Results: The latex agglutination test (LAT), electropherotyping (RNA-PAGE), and reverse transcription polymerase chain reaction revealed positivity rates of 39.13%, 20.30%, and 37.70%, respectively. RNA-PAGE identified 11 bands with a 4:2:3:2 migration pattern, indicative of the segmented genome of BRVA. BRVA was successfully isolated from LATpositive samples, with 26 samples exhibiting clear cytopathic effects upon passage in MA-104 cell lines. Genotyping identified G10 as the predominant G genotype, with P[11] genotypes comprising 76.92% of the isolates. The most common G/P combination was G10P[11], highlighting its zoonotic potential. Conclusions and Relevance: These findings underscore the importance of molecular detection and genotyping for effective vaccine development. This study provides crucial insights into the prevalent G and P genotypes of BRVA in Gujarat, India, aiding in the development of targeted control measures.

• Journal of the Korean Institute of Landscape Architecture
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• v.35 no.2 s.121
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• pp.71-80
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• 2007

Korea began preserving and managing natural monuments in 1933 under Japanese Colonization, but North Korea and South Korea were forced to establish separate natural monument management policies because of the division after the Korean Independence. The purpose of this study is to compare and analyze the natural monument management policies of both south and North Korea between 1933 and 2005 to introduce new policies for Korea unification. The following are the results: First, South Korea manages every type of cultural asset, including natural monuments, through the 'Cultural Heritage Protection Act,' whereas North Korea managing its cultural assets through the 'Cultural Relics Protection Act' and the 'Landmark/Natural Monument Protection Act.' Second, South Korea preserves and utilizes natural monuments for the purpose of promoting the cultural experience of Korean people and contributing to the development of world culture, whereas North Korea uses its natural monuments to promote the superiority of socialism and protect its ruling power. Third, North and South Korea have similar classification systems for animals, plants, and geology, but North Korea classifies geography as one of its natural monuments. Unlike South Korea, North Korea also designates imported animals and plants not only for the preservation and research of genetic resources, but also for their value as economic resources. Fourth, North Korea authorizes the Cabinet to designate and cancel natural monuments, whereas South Korea designates and cancels natural monuments by the Cultural Heritage Administration through the deliberation of a Cultural Heritage Committee. Both Koreas' central administrations establish policies and their local governments carry them out, while their management systems are quite different. In conclusion, it is important to establish specified laws for the conservation of natural heritages and clarified standards of designation in order to improve the preservation and management system and to sustain the diversity of natural preservation. Moreover it is also necessary to discover resources in various fields, designate protection zones, and preserve imported trees. By doing so, we shall improve South Korea's natural monument management policies and ultimately enhance national homogeneity in preparation for the reunification of the Koreas in the future.

• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.17-22
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• 2008

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

• Korean Journal of Environment and Ecology
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• v.35 no.6
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• pp.632-643
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• 2021

The objective of this study was to examine vegetation community structure and distribution of Fraxinus chiisanensis in Mt.Minjuji of Chungcheongbuk-do by setting up and surveying 8 plots (400 m² each). Mean Importance Value (MIV) of Fraxinus chiisanensis in 8 plots was 35.19% in average (ranging from 26.07~42.74%). Since it is the dominant species in all plots, it is expected to maintain the present vegetation structure. The analysis of the DBH (diameter at breast height) showed that the diameter of Fraxinus chiisanensis in Mt.Minjuji ranges from 2 to 43cm. The majority of Fraxinus chiisanensis is expected to maintain current state unless disturbance or rapid environmental change occurs. The Species Diversity (H') was 0.8498~1.0261, Evenness (J') was 0.8160~0.9256, Dominance Index (D) was 0.0789~0.1840, Maximum Diversity (H'max) was 1.0414~1.2041. The analysis of annual ring and radial growth showed that the average age of Fraxinus chiisanensis in Mt.Minjuji was 29.1years(ranging from 22~58years). The average annual radial growth of Fraxinus chiisanensis was the highest in community G with 5.84mm and the lowest in community B with 2.80mm. The similarity index analysis revealed that the similarity index between community B and E, C and F, H was the highest with 69.0%, and the similarity index between community E and F was the lowest with 29.6%. Both the area of Fraxinus chiisanensis community of Mt.Minjuji and its population size are very small. Therefore, this area needs to be designated as Forest Genetic Resource Reserve.

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.137-145
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• 2005

Traditional screening techniques have missed up to 99% of microbial resources existing in the nature. Strategies of direct cloning of environmental DNAs comprising tine genetic blueprints of entire microbial metagenomes provide vastly more genetic information than is contained in the culturable. Therefore, one way to screening the useful gene in a variety of environments is the construction of metagenomic DNA library. In this study, the water samples were collected from Daecheong Reservoir in the mid Korea, and analyzed by T-RFLP to examine the diversity of the microbial communities. The crude DNAs were extracted by SDS-based freezing-thawing method and then further purified using an $UltraClean^{TM}kit$ (MoBio, USA). The metagenomic libraries were constructed with the DNAs partially digested with EcoRI, BamHI, and SacII in Escherichia coli DH10B using the pBACe3.6 vector. About 14.0 Mb of metagenomic libraries were obtained with average inserts 13 ${\sim}$ 15 kb in size. The genes responsible for degradation of 1, 2-dichloroethane (1, 2-DCE) via hydrolytic dehalogenation were identified from the metagenomic libraries by colony hybridization. The 1, 2-dichloroethane dehalogenase gene (dhlA) was cloned and its nucleotide sequence was analyzed. The activity of the 1, 2-DCE dehalogenase was highly expressed to the substrate. These results indicated that the dhlA gene identified from the metagenomes derived from Deacheong Reservoir might be useful to develop a potent strain for degradation of 1, 2-DCE.

• Korean Journal of Poultry Science
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• v.46 no.3
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• pp.173-183
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• 2019

Based on their virulence, the avian influenza viruses (AIVs) are classified into two pathotypes: low pathogenic avian influenza (LPAI) virus and highly pathogenic avian influenza (HPAI) virus. Among the 16 HA subtypes of AIV, only the H5 and H7 subtypes are classified as HPAI. Some AIVs, including H5 and H7 viruses, can infect humans directly. Six H7 subtype isolates from wild birds of the H7N7 (n=4) and H7N1 (n=2) subtypes were characterized in this study. Phylogenetic analysis showed that eight viral genes (HA, NA, PB2, PB1, PA, NP, M, and NS) of the H7 isolates clustered in the Eurasian lineage, the genetic diversity of which is indicated by its division into several sublineages. The Korean H7 isolates had two motifs, PEIPKGR and PELPKGR, at the HA cleavage site, which have been associated with LPAI viruses. Six H7 isolates encoded glutamine (Q) and glycine (G) at positions 226 (H3 numbering) and 228 of HA, suggesting avian-type receptor-binding specificity. None of the Korean H7 isolates had the amino acid substitutions E627K in PB2 and I368V in PB1, which are critical for efficient replication in human cells. The Korean H7 isolates showed no deletions in the NA stalk region and in NS. These results suggest that the Korean H7 isolates from wild birds are different from the H7N9 influenza viruses isolated in China in 2013, which are capable of infecting humans.

• Asian Journal of Turfgrass Science
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• v.20 no.1
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• pp.77-82
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• 2006

Annual bluegrass(Poa annua L.) is a problem weed that is very difficult to control on golf courses. There are some reasons that make Poa annua such a difficult weed to be controled. One of these reasons is this plant's ability to reproduce its seed even under stressful conditions. Another reason is its adaptation to low mowing heights. Above all things, the greatest competitive advantage of Poa annua is its genetic diversity. Generally, Poa annua is a bunch type and annual type cool-season grass, but some types act as weak perennials and have stolons. There has been much research on controlling annual bluegrass in golf course turf with chemical and cultural techniques. This research has been conducted for more than 85 years. There has been some progress in controlling some types of Poa annua, but these methods have not been successful on every biotype. Among all of the techniques, Roundup ready creeping bentgrass has the most promise of controlling the diverse types of Poa annua. Roundup ready bentgrass is capable of tolerating the effects of Roundup(glyphosate) while it kills other plants including Poa annua. By using this new technology, we can make Poa annua free greens, tees, and fairways.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.74-82
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• 2014

A perennial plant species of Ranunculaceae, Pulsatilla tongkangensis is a very rare species only found in limestone vegetation of South Korea. It is not only rare but also has great value as a horticultural resource, thus resulting in serious problems of overexploitation and habitat disturbance. For the conservation of the rare species and its development as a resource, the study was conducted with the following three aims: first, to investigate any new geographical distribution of the species; second, to identify the site condition and vegetation structure of its habitat; and third, to compare the ecology of P. tongkangensis in other microhabitats such as rock, slope and ridge area. A new distribution was found in limestone vegetation at Danyang-gun, Chungcheongbuk-do. It was, therefore, confirmed that the distribution of P. tongkangensis exactly coincides with the area of distribution of limestone, indicating a possibility that the species might be a calcicole. 87% of the microhabitats found in rocks. Compared to P. tongkangensis, at ridges or slopes, number of P. tongkangensis plants were few in rocks. It is possibly due to the lack of microhabitats that could settle in rocky areas. Once established on rocks, however, plants grew large enough to flower with higher number of leaves and flowers per plant, resulting in higher flowering rate on rocky areas. Cracks in/between rocks are likely to be safe places for the plants with favorable light conditions, abundant fine particles to root down and reduced competition for resources. Yet, further research is necessary in terms of determining whether the species is indeed a calcicole, whether other limiting factors work in its establishment stage, and genetic diversity exists in the population.

• Journal of Veterinary Clinics
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• v.27 no.5
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• pp.501-507
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• 2010

The aim of the current study was to analyze the diversity of the major surface protein (Msp) gene in Theileria buffeli, which is known as the major antigenic protein recognized by the immune system of the host. In addition, we characterized the diversification of the Msp gene and its relationship to with the pathogenicity of Theileria. Complete blood counts (CBC) and Theileria 18S rRNA PCR sequence analysis were performed for 177 Korean indigenous cattle (KIC) in Jeju Island. A total of 28 KIC (16 anemic and 12 non-anemic KIC) were then randomly selected based on 18s rRNA PCR positive samples for sequence analysis of the Theileria Msp gene, which was performed twice for each specimen. The resulting 56 Msp gene sequences were classified into five antigenicity types (type I to V), according to the variable region (517-571 bp), which exhibited high similarity (${\geq}$ 98.9%) to several available GenBank sequences (Theileria spp. from China-EU584237; T. sergenti from China-DQ078264; Theileria spp. from Thailand-AB081329; Theileria spp. from Japan-AB218442; T. sergenti from Japan-AB016280). The 56 Msp sequences consisted of 22, 15, 9, 8, and 2 cases of type I to type V Msp genes, respectively. The most prevalent type in both anemic and non-anemic KIC was type I (37.5% in anemic and 41.7% in non-anemic). Among the remaining types, type II was the most prevalent (37.5%) in anemic KIC, while type IV was the most prevalent (25%) in non-anemic KIC. The results of our study help confirm the diversity of Msp gene types and demonstrate that the gene type distribution of Msp genes varies among Theileria-infected KIC in Jeju Island.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.81-88
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• 2007

The evaluation of soybean germplasm has mainly been carried out by morphological characters at Genetic Resources Division, Rural Development Administration (RDA). However, this information has been limited serving a diverse information for user and effectively managing the soybean germplasm. To resolve this problem, soybean collection conserved at RDA gene bank was profiled using nine soybean SSR (Simple Sequence Repeat) markers. Soybean SSR allele was confirmed using genescan and genotyper softwares of automatic sequencer for accurate genotyping of each accession and continuous accumulation of data. SSR profiling of soybean germplasm has been carried out from 2,855 (Satt458) to 4,368 (Satt197) accessions by locus. The number of allele revealed 267 with an average of 29.6 in total accession, and varied from a low of 21 (Satt532 and Satt141) to a high of 58 (Sat_074). Although the number of accessions of wild soybean is less than that of soybean landraces, Korean wild soybean is more variable than other soybean landraces populations in total number of alleles. However, Korean soybean landraces were more variable than Korean wild soybeans in 5 loci. In the allele frequency, wild soybean accessions showed an even distribution in all alleles and higher distribution in low ladder than in high ladder. Also, Korean soybean landraces revealed a high condensed frequency in Satt286 (202 bp, 232 bp), Chinese soybean landraces in Satt197 (171 bp) and Satt458 (173 bp), and Japanese soybean landraces in Sat_074 (244 bp) and Satt458 (170 bp). These SSR profile information will be provided as indications of redundancies or omissions of accessions and can aid in managing soybean collection held at RDA gene bank. The information on diversity analysis could help to enlarge the genetic diversity of materials in breeding program, and could be used to develop a core collection of soybean germplasm.