• 한국발생생물학회지:발생과생식
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• 제16권1호
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• pp.47-51
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• 2012

Genomic DNAs (gDNAs) were isolated from the hard clam ($Meretrix$ $lusoria$, Roding, 1798) populations of Gunsan located in the Yellow Sea of the Korean peninsula. Genetic distances among different individuals of the LSCP (light shell color population) population of the hard clam (lane 1-11), GSCP (grey shell color population) population of the hard clam (lane 12-22) and DSCP (dark shell color population) population of the hard clam (lane 23-33), respectively, were generated using Systat version 10 according to the bandsharing values and similarity matrix. The dendrogram, generated by seven reliable oligonucleotides primers, indicates 3 genetic clusters. LSCP population could be evidently discriminated with the other two populations among three populations. The longest genetic distance (0.801) was found to exist between individuals in the two populations, between individuals' no. 33 of the DSCP population and no. 06 of the LSCP population. The higher fragment sizes (>2,000 bp) are much more observed in the GSCP population. Three hard clam populations can be clearly distinguished, especially, by their morphological characters and PCR-based approach.

• Archives of Pharmacal Research
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• 제27권12호
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• pp.1270-1274
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• 2004

Random amplified polymorphic DNA (RAPD) analysis was used to determine the genetic relationships among seventeen species of the Acanthopanax species. The DNA isolated from the leaves of the samples was used as template in polymerase chain reaction (PCR) with twenty random decamer primers in order to distinguish plant subspecies at the level of their genomes. The RAPD patterns were compared by calculating pairwise distances using Dice similarity index, and produced to the genetic similarity dendrogram by unweighted pair-group method arithmetic averaged (UPGMA) analysis, showing three groups; a major cluster(twelve species), minor cluster (4 species) and single-clustering species. The results of RAPD were compatible with the morphological classification, as well as the chemotaxonomic classification of the Acanthopanax species. The Acanthopanax species containing 3,4-seco-lupane type triterpene compounds in their leaves corresponded to the major cluster, another species having oleanane or normal lupane type constituents to minor clusters, and one species not containing triterpenoidal compound to single-cluster.

• Journal of Crop Science and Biotechnology
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• 제10권2호
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• pp.106-111
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• 2007

Increasing genetic variability with mutagenic agents has been broadly employed in plant breeding because it has the potential to alter one or more desirable traits. In this study, a molecular analysis assessed by Amplified Fragment Length Polymorphisms(AFLPs) and a morphological analysis based on seedlings subjected to aluminum stress were compared. Also, an analysis of allelic frequencies was performed to observe unique alleles present in the pool. Genetic distances ranging from 0.448 to 0.953 were observed, suggesting that mutation inducing was effective in generating variability. The genetic distances based on morphological data ranged from 0(genotypes 22 and 23) to 30.38(genotypes 15 and 29). In the analysis of allelic frequency, 13 genotypes presented unique alleles, suggesting that mutation inducing was also targeting unique sites. Mutants with good performance under aluminum stress(9, 15, 18 and 27) did not form the same clusters when morphological and molecular analyses were compared, suggesting that different genomic regions may be responsible for their better performance.

• 농업과학연구
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• 제48권3호
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• pp.505-514
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• 2021

Among non-timber forest products, Tricholoma matsutake is a high value added item. Many countries, including Korea, China, and Japan, are doing research and technology development to increase artificial cultivation and productivity. However, the production of T. matsutake is on the decline due to global warming, abnormal temperatures and pine tree pest problems. Therefore, it is necessary to identify trends in domestic and foreign research on T. matsutake, respond to preemptive research and development to preserve the genetic resources of T. matsutake and increase its productivity. Based on the correlation between keywords in the high frequency keywords, it was observed that microbial clusters of T. matsutake are mainly found in Korea. The main focus in China has been the pharmacology studies on the ingredients of T. matsutake. The main focus in Japan has been on preserving the genetic diversity and species of T. matsutake. Thus, future domestic studies of T. matsutake will require pharmacological studies on the ingredients of T. matsutake and on its genetic diversity and species conservation. In addition, unlike China and Japan, genetic keywords did not appear in Korea at high frequency. Therefore, Korea will have to proceed with research using modern molecular biology techniques.

• Journal of Forest and Environmental Science
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• 제38권1호
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• pp.12-20
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• 2022

Pongamia (Pongamia pinnata L.) as a source of non-edible oil, is potential tree species for biodiesel production. For several reasons, both technical and economical, the potential of P. pinnata is far from being realized. The exploitation of genetic diversity for crop improvement has been the major driving force for the exploration and ex situ/in situ conservation of plant genetic resources. However, P. pinnata improvement for high oil and seed production is not achieved because of unsystematic way of tree improvement. Performance of P. pinnata planted by Karnataka Forest Department was assessed based on yield potential by collecting 157 clones out of 264 clones established by Karnataka Forest Department research wing under different research circles/ranges. It was evident that the all the seed and pod traits were significantly different. Further, selection of superior germplasm based on oil and pod/seed parameters was achieved by application of Mahalanobis statistics and Tocher's technique. On the basis of D² values for all possible 253 pairs of populations the 157 genotypes were grouped into 28 clusters. The clustering pattern showed that geographical diversity is not necessarily related to genetic diversity. Cluster means indicated a wide range of variation for all the pod and seed traits. The best cluster having total oil content of more than 34.9% with 100 seed weight of above 125 g viz. Cluster I, II, III, IX, XV, XIX, XXI, XXIII, XXVI and XXVII were selected for clonal propagation.

• 한국발생생물학회지:발생과생식
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• 제26권3호
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• pp.127-133
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• 2022

The seven oligonucleotides primers were consumed to produce the quantity of unique loci shared to each pen shell team (ULSEPT) and quantity of loci shared by the binary pen shell teams. 154 quantities of LSBPP, with a mediocre of 22.0 per primer, were noticed in the binary pen shell (Atrina pectinata) teams. 328 fragments were recognized in the pen shell team A (PSTA), and 257 in the pen shell team B (PSTB): 77 quantities of ULSEPT (23.48%) in the PSTA and 121 (47.08%) in the PSTB. The band-sharing amount (BS amount) between entity's no. 01 and no. 05 was the highest (0.884) between the binary PSTs. The median band-sharing amount of entities in the PSTA (0.685±0.011) was higher than in those invented from the PSTB (0.640±0.009) (p<0.05). The highest genetic distance presenting substantial molecular difference was between entities PECTINATA no. 06 and PECTINATA no. 04 (0.498). Through this study, it is possible a certain degree to contribute to increasing the cultivation of pen shells, conservation of species, protection of the natural environment, and preservation of ecosystems.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.312-319
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• 2017

The need to preserve and use plant genetic resources is widely recognized, and the prospect of dwindling plant genetic diversity, coupled with increased demands on these resources, has made them a topic of global discussion. In the present study, the genetic diversity and population structure of 73 ginseng accessions collected from six regions in China were analyzed using eight simple sequence repeat (SSR) markers. Major allele frequencies ranged between 0.38 ~ 0.78, with a mean allele frequency value of 0.571. The number of alleles discovered ranged from 3 to 10 per accession, with a mean number of 7; 56 alleles were discovered in total. Gene diversity (GD) and polymorphic information content (PIC) values were similar to each other, and they ranged from 0.36 ~ 0.77 (mean 0.588) and 0.33 ~ 0.74 (mean 0.548), respectively. Accessions were divided into three clusters based on their phylogenetic relationships and genetic similarities, and although the populations were similar, they were not classified according to the region. Regional genetic diversity was also similar, with slight differences observed based on the number of accessions per region. It is expected that the findings of the present study can provide basic data for future studies on ginseng genetic diversity and for breeding ginseng cultivars.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1871-1880
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• 2018

Objective: Recently, there has been an increasing interest in conservation of native genetic resources of chicken on a worldwide basis. Most of the native chicken breeds are threatened by extinction or crossing with ecotypes. Methods: Six Saudi native chicken breeds including black naked neck, brown frizzled, black, black barred, brown and gray were used in the current study. The aim of the current study was to evaluate genetic diversity, relationship and population structure of Saudi native chicken breeds based on 20 microsatellite markers. Results: A total of 172 alleles were detected in Saudi native chicken breeds across all 20 microsatellite loci. The mean number of alleles per breed ranged from 4.35 in gray breed to 5.45 in normally feathered black with an average of 8.6 alleles. All breeds were characterized by a high degree of genetic diversity, with the lowest heterozygosity found in the brown breed (72%) and the greatest in the frizzled and black barred populations (78%). Higher estimate of expected heterozygosity (0.68) was found in both black breeds (normal and naked neck) compared to the other chicken populations. All studied breeds showed no inbreeding within breed (negative inbreeding coefficient [$F_{IS}$]). The phylogenetic relationships of chickens were examined using neighbor-joining trees constructed at the level of breeds and individual samples. The neighbor-joining tree constructed at breed level revealed three main clusters, with naked neck and gray breeds in one cluster, and brown and frizzled in the second cluster leaving black barred in a separate one. Conclusion: It could be concluded that the genetic information derived from the current study can be used as a guide for genetic improvement and conservation in further breeding programs. Our findings indicate that the Saudi native chicken populations have a rich genetic diversity and show a high polymorphism.

• 한국패류학회지
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• 제24권1호
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• pp.11-24
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• 2008

GDNA was isolated from the jedo venus clam (Protothaca jedoensis, Lischke) from Boryeong (jedo venus clam from Boryeong JVCB) and Wonsan (jedo venus clam from Wonsan; JVCW) located in the West Sea and the East Sea of Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the populations genetic variations. In the present study, the seven decamer primer generated the one hundred and eleven major/minor specific bands in JVCB population and ninety four-specific bands in JVCW population. Seven primers generated the unique shared bands to each population of one hundred and seventy-six, on average of 25,1, in JVCB population from Boryeong and three hundred thirty, on average of 47,1, in JVCW population from Wonsan, respectively. The dendrogram obtained by the seven oligonucleotides primers, indicates two genetic clusters. Especially, two Protothaca between the individual WONSAN no. 12 and BORYEONG no. 10 showed the longest genetic distance (0.537) in comparison with other individuals used. Accordingly, RAPD analysis showed that the JVCB was a little more genetically diverse than the JVCW population. This result implies the genetic similarity owing to rearing in the same and/or similar circumstances or inbreeding within the JVCW population. So to speak, JVCB population may have high levels of genomic DNA variability owing to the introduction of the wild individuals from the other sites to sampling sites although it may be the geographically diverse distribution of this species. However, it was confirmed that it did not appear like that really in this study. We feel convinced that RAPD analysis discovered a significant genetic distance between two Protothaca population pairs (P<0.001). The existence of population discrimination and genetic diversity between two Protothaca populations was identified by RAPD analysis.

• Molecules and Cells
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• 제25권2호
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• pp.301-304
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• 2008

Microsatellites, short tandem repeats, are useful markers for genetic analysis because of their high frequency of occurrence over the genome, high information content due to variable repeat lengths, and ease of typing. To establish a panel of microsatellite markers useful for genetic studies of the Korean population, the allele frequencies and heterozygosities of 207 microsatellite markers in 119 unrelated Korean, Indian and Pakistani individuals were compared. The average heterozygosity of the Korean population was 0.71, similar to that of the Indian and Pakistani populations. More than 80% of the markers showed heterozygosity of over 0.6 and were valuable as genetic markers for genome-wide screening for disease susceptibility loci in these populations. To identify the allelic distributions of the multilocus genetic data from these microsatellite markers, the population structures were assessed by clustering. These markers supported, with the most probability, three clustering groups corresponding to the three geographical populations. When we assumed only two hypothetical clusters (K), the Korean population was separate from the others, suggesting a relatively deep divergence of the Korean population. The present 207 microsatellite markers appear to reflect the historical and geographical origins of the different populations as well as displaying a similar degree of variation to that seen in previously published genetic data. Thus, these markers will be useful as a reference for human genetic studies on Asians.