• Title/Summary/Keyword: Genetic Similarity

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DNA fingerprinting analysis of maize varieties and parental lines using microsatellite markers (Microsatellite 마커를 이용한 옥수수 품종 및 자식 계통에 대한 DNA Fingerprinting 분석)

  • Kwon, Yong-Sham
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.367-375
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    • 2016
  • In the present study, we conducted genetic characterization of 90 commercial maize varieties and parental lines using microsatellite markers. Thirteen microsatellite markers were selected from 100 primer pairs in the maize genome data on the basis of polymorphism information contents (PIC) value and distinct amplification products. These markers detected 5 to 24 alleles, with an average of 13.69. The mean PIC value was 0.865 and ranged from 0.716 to 0.942. The unweighted pair-group method with arithmetical average (UPGMA) analysis was conducted for constructing the dendrogram using Jaccard's genetic similarity coefficient. The genetic similarity varied from 0.07 to 0.824. Thirteen microsatellite markers identified all 90 maize varieties and parental lines. The maize varieties were clustered into 5 major groups consistent with type and pedigree information. The microsatellite profile database of maize varieties could be used to select comparative varieties through genetic relationship analysis between existing varieties and candidate varieties in distinctness tests.

Genetic Relationship of Pleurotus ferulae Strains (아위버섯(Pleurotus ferulae) 균주의 유전적 유연관계)

  • Choi, Jae-Sun;Lee, Dong-Hee;Chang, Hu-Bong;Kang, Bo-Gu;Koo, Chang-Duck
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.28-32
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    • 2009
  • This study was carried out to investigate the genetic relationship of Pleurotus ferulae, an edible mushroom found on a medicinal plant, Ferula assa-foetida, in central China. The genetic relationships of 15 Pleurotus species strains, including five P. ferulae strains were analyzed. The strains were divided into seven groups at 80% genetic similarity level according to random amplified polymorphic DNA (RAPD) analysis. Four out of the seven groups consisted of two to four strains, while the other three groups consisted of three strains. In each of the three groups, the three strains were from each of three different Pleurotus species (P. cornucopiae, P. florida and P. sajorcaju). Other strains grouped together for genetic similarity were P. eryngii 26060 and P. fuscus var. ferulae 26065, three strains of P. ostreatus, and four P. ferulae strains (Bakdal, Awi, Cheonsan 1, and Yesan). However, Japanese Seolyi which belongs to P. ferulae and Heukpyung which belongs to P. ostreatus were together in a separate group.

Genetic Relationship of the Five Venerid Clams유 (Bivalvia, Veneridae) in Korea (한국산 백합과 5종의 유전적 유연관계)

  • 정형택;김정;신종암;서호영;최상덕
    • Journal of Aquaculture
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    • v.17 no.4
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    • pp.251-257
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    • 2004
  • The random amplified polymorphic DNAs (RAPD) technique was used to characterize the genetic relationship of five species from in the family of Veneridae which is one of the commercially important clam family in Korea. The veneride clams' DNA were extracted from adductor muscular by the proteinase K-phenol method. Among 20 primers, 15 unit primers were amplified and produced at least, 2 or 3 from the top band. Genetic similarity between the purplish washington, Saxidomus purpuratus and the hard clam, Meretrix lusoria was the highest (0.87); the lowest genetic similarity (0.46) was formed between the little clam, Ruditapes philppinarum and the purplish washington, S. purpuratus. The genetic relationship between the venus clam, Protothaca jedoensis and the little clam, R. philppinarum was a closer than those between others. These results may indicate that the method of artificial seeding production of P. jedoensis for the propagation of resources can be focused on R. philppinarum.

Genetic Similarity and Difference of Marsh Clam (Corbicula leana) Obtained by RAPD-PCR

  • Yoon, Jong-Man;Park, Hwan-ha;Choe, Sun-Nam
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2002.05a
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    • pp.279-280
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    • 2002
  • Genomic DNA from the muscle of marsh clam (Corbicula leana) from Gochang was extracted in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and/or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35 kb) identifying individuals was observed in lane 22.

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Genetic Similarity and Difference of Marsh Clam (Corbicula leana) Obtained by RAPD-PCR

  • Yoon, Jong-Man;Park, Kwan-Ha;Choe, Sun-Nam
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2002.08a
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    • pp.171-172
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    • 2002
  • Genomic DNA from the muscle of marsh clam (Corbicula leana)from Gochang was extrected in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and-or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35kg) identifying individuals was observed in lane 22.

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Clustering Parts Based on the Design and Manufacturing Similarities Using a Genetic Algorithm

  • Lee, Sung-Youl
    • Journal of Korea Society of Industrial Information Systems
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    • v.16 no.4
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    • pp.119-125
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    • 2011
  • The part family (PF) formation in a cellular manufacturing has been a key issue for the successful implementation of Group Technology (GT). Basically, a part has two different attributes; i.e., design and manufacturing. The respective similarity in both attributes is often conflicting each other. However, the two attributes should be taken into account appropriately in order for the PF to maximize the benefits of the GT implementation. This paper proposes a clustering algorithm which considers the two attributes simultaneously based on pareto optimal theory. The similarity in each attribute can be represented as two individual objective functions. Then, the resulting two objective functions are properly combined into a pareto fitness function which assigns a single fitness value to each solution based on the two objective functions. A GA is used to find the pareto optimal set of solutions based on the fitness function. A set of hypothetical parts are grouped using the proposed system. The results show that the proposed system is very promising in clustering with multiple objectives.

AFLP Fingerprinting of Brassica campestis L. ssp. napus var. nippo-oleifera Makino from Korea

  • Huh, Man-Kyu;Huh, Hong-Wook
    • Animal cells and systems
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    • v.5 no.2
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    • pp.101-106
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    • 2001
  • AFLPS (amplified fragment length polymorphisms) were used to estimate the genetic diversity of seven populations of Brassica campestis L. ssp. napus var. nippo-oleifera Makino between naturalized and cultivated populations. The seven Korean populations maintained a high level of genetic diversity. For example, all eight primers were high polymorphic, with an average of 3.2 effective alleles per primer set, and the expected heterozygosity was also high. The majority of genetic variance resided within populations The combinations of an insect-pollinated, outcrossing breeding system, large populations sizes, a high degree of gene flow and a propensity for high fecundity may explain the high level of genetic diversity within cultivated populations. Estimates of genetic similarity on the proportion of shared fragments ranged from 0.952 to 0.999. The high level of gene flow In Korean naturalized populations is mainly caused by seed dispersal via sea tide and the gene flow of cultivated populations may be enhanced in part by artificial pollen dispersal.

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Development of Similarity-Based Document Clustering System (유사성 계수에 의한 문서 클러스터링 시스템 개발)

  • Woo Hoon-Shik;Yim Dong-Soon
    • Proceedings of the Society of Korea Industrial and System Engineering Conference
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    • 2002.05a
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    • pp.119-124
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    • 2002
  • Clustering of data is of a great interest in many data mining applications. In the field of document clustering, a document is represented as a data in a high dimensional space. Therefore, the document clustering can be accomplished with a general data clustering techniques. In this paper, we introduce a document clustering system based on similarity among documents. The developed system consists of three functions: 1) gatherings documents utilizing a search agent; 2) determining similarity coefficients between any two documents from term frequencies; 3) clustering documents with similarity coefficients. Especially, the document clustering is accomplished by a hybrid algorithm utilizing genetic and K-Means methods.

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Software Similarity Measurement based on Dependency Graph using Harmony Search

  • Yun, Ho Yeong;Joe, Yong Joon;Jung, Byung Ok;Shin, Dong myung;Bahng, Hyo Keun
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.12
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    • pp.1-10
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    • 2016
  • In this paper, we attempt to prevent certain cases by tracing a history and making genogram about open source software and its modification using similarity of source code. There are many areas which use open source software actively and widely, and open source software contributes their development. However, there are many unconscious cases like ignoring license or intellectual properties infringe which can lead litigation. To prevent such situation, we analyze source code similarity using program dependence graph which resembles subgraph isomorphism problem, a typical NP-complete problem. To solve subgraph isomorphism problem, we utilized harmony search of metaheuristic algorithm and compared its result with a genetic algorithm. For the future works, we represent open source software as program dependence graph and analyze their similarity.