• 생명과학회지
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• 제13권3호
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• pp.280-290
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• 2003

RAPD 연관지도를 RFLP 연관지도와 합병을 하는 것은 각각의 유전 marker들의 단점을 서로 보완하여 세밀화된 유전자 지도작성을 용이하게 할 수 있다. 본 연구는 Essex와 PI 437654의 $F_2$ 및 $F_3$ 후대계통들을 재료로 하여 작성된 RAPD 연관지도를 콩의 RFLP 연관지도와 합병을 함에 있어서 나타난 몇가지 특징들을 기술하고자 함을 목적으로 하는 바 그 특징들은 아래와 같이 요약된다. 1. RAPD 연관지도상에서의 RFLP probe들의 위치가 RFLP 연관지도상에서의 위치와 부분적으로 변동된 현상이 나타났다. RAPD 연관그룹 L.G.C-3을 RFLP 연관그룹 a1 및 a2와 합병하는 과정에서 pSAC3와 pA136, 그리고 pA170/EcoRV와 pB170/HindIII이 서로 반대방향으로 위치하였다. pK400은 RFLP 연관지도상에서는 pA96-1과 pB172의 사이에 위치한 반면 RAPD 연관지도상에서는 i locus와 pA85 사이에 위치하였다. 2. RAPD 연관지도상에서의 두 marker들간의 간격이 RFLP 연관지도상에서의 간격보다 멀어진 현상이 두드러지게 나타났다. pA890과 pK493간의 간격은 RAPD 연관그룹 L.G.C-1에서는 48.6 cM이었던 반면 RFLP 연관 그룹상에서는 단지 13.3 cM으로 나타났다. 또한 pB32-2와 pA670, pA670과 pA668사이의 간격은 RAPD 연관그룹 L.G.C-2에서는 50.9 cM과 31.7 cM이었던 반면, RFLP 연관지도상에서의 간격은 각각 35.9 cM과 13.5 cM으로 나타났다. 3. 하나의 RFLP probe로부터 두개 이상의 다형화 현상을 나타낸 marker들이 동일한 연관그룹이나 다른 연관그룹에 위치하는 현상이 나타났다. 제한효소 HindIII로 절단된 probe pK418은 세개의 marker를 나타내었는데, 그 중 하나는 L.G.C-20에 위치하였으며, 다른 두개는 L.G.C-4에 위치하였다. 위에 나타난 특징들은 RAPD 연관지도는 intraspecific cross의 후대계통들을 재료로 하여 작성된 반면 RFLP 연관지도는 interspecific cross의 후대계통들을 재료로 하여 작성된 결과에선 비롯된 차이점 때문인 것으로 추측된다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.663-665
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• 2010

By the curing and transformation experiment, it was found thatthe genes of Pseudomonas sp.KU171(pKU19) for MCPA-degrading were located on a plasmid pKU19. Also the plasmid had degradative gens for 2,4-D, 3CB, and DCP. Molecular size of pKU19 was measured to be 31.2Kb. The restriction pattern were analyzed with Eco RI, BglII,XhoI, and the restriction map was generated.

• BMB Reports
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• 제46권8호
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• pp.416-421
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• 2013

Genome-wide association studies (GWAS) have become popular as an approach for the identification of large numbers of phenotype-associated variants. However, differences in genetic architecture and environmental factors mean that the effect of variants can vary across populations. Understanding population genetic diversity is valuable for the investigation of possible population specific and independent effects of variants. EvoSNP-DB aims to provide information regarding genetic diversity among East Asian populations, including Chinese, Japanese, and Korean. Non-redundant SNPs (1.6 million) were genotyped in 54 Korean trios (162 samples) and were compared with 4 million SNPs from HapMap phase II populations. EvoSNP-DB provides two user interfaces for data query and visualization, and integrates scores of genetic diversity (Fst and VarLD) at the level of SNPs, genes, and chromosome regions. EvoSNP-DB is a web-based application that allows users to navigate and visualize measurements of population genetic differences in an interactive manner, and is available online at [http://biomi.cdc.go.kr/EvoSNP/].

• 스마트미디어저널
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• 제13권2호
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• pp.129-135
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• 2024

이 연구에서는 MAP-Elites 알고리즘의 연속 최적화 성능을 향상한 새로운 접근법을 제안한다. 기존의 자기 참조 MAP-Elites 알고리즘은 차분 진화 알고리즘의 "DE/rand/1/bin" 연산자를 사용했는데, 이 연산자는 회전 불변이 아니라서 각 변수 간의 상관관계가 높은 경우 성능이 감소하는 문제가 존재한다. 제안하는 알고리즘은 "DE/rand/1/bin" 연산자 대신에 "DE/current-to-rand/1" 연산자를 사용한다. 이 연산자는 회전 불변성을 가지므로 각 변수 간의 상관관계가 높은 분리 불가능 최적화 문제에서도 강건한 성능을 보장할 수 있다. 실험 결과, 제안하는 알고리즘이 비교 알고리즘들에 비해 높은 성능을 발휘함을 확인했다.

• BMB Reports
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• 제50권11호
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• pp.535-536
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• 2017

A novel approach has been used to identify functional interactions relevant to human disease. Using high-throughput human-yeast genetic interaction screens, a first draft of disease interactome was obtained. This was achieved by first searching for candidate human disease genes that confer toxicity in yeast, and second, identifying modulators of toxicity. This study found potentially disease-relevant interactions by analyzing the network of functional interactions and focusing on genes implicated in amyotrophic lateral sclerosis (ALS), for example. In the subsequent proof-of-concept study focused on ALS, similar functional relationships between a specific kinase and ALS-associated genes were observed in mammalian cells and zebrafish, supporting findings in human-yeast genetic interaction screens. Results of combined analyses highlighted MAP2K5 kinase as a potential therapeutic target in ALS.

• 대한산업공학회지
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• 제20권3호
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• pp.15-34
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• 1994

This paper considers the genetic algorithms(GAs) for the mixed model assembly line sequencing(MMALS) in which the objective is to minimize the overall line length. To apply the GAs to the MMALS, the representation, selection, genetic sequencing operators, and genetic parameters are studied. Especially, the existing sequencing binary operators such as partially map crossover(PMX), cycle crossover(CX), and order crossover (OX) are modified to be suitable for the MMALS, and a new sequencing binary operator called immediate successor relationship crossover (ISR) is introduced. These binary operators mentioned above and/or unary operators such as swap, insertion, inversion, displacement, and splice are compared to find operators which work well in the MMALS. Experimental results indicate that 1) among the binary operators ISR operator is the best, followed by the modified OX, and the modified PMX, with the modified CX being the worst, 2) among the unary operators inversion operator is the best, followed by displacement, swap, and insertion, with splice being the worst, and 3) in general, the unary operators perform better than the binary operators for the MMALS.

• Journal of applied mathematics & informatics
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• 제20권1_2호
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• pp.327-343
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• 2006

A general methodology is suggested to solve shelf-space allocation problem of retailers. A multi-item inventory model of breakable items is developed, where items are either complementary or substitute. Demands of the items depend on the amount of stock on the showroom and unit price of the respective items. Also demand of one item decreases (increases) due to the presence of others in case of substitute (complementary) product. For such a model, a Contractive Mapping Genetic Algorithm (CMGA) has been developed and implemented to find the values of different decision variables. These are evaluated to have maximum possible profit out of the proposed system. The system has been illustrated numerically and results for some particular cases are derived. The results are compared with some other heuristic approaches- Simulated Annealing (SA), simple Genetic Algorithm (GA) and Greedy Search Approach (GSA) developed for the present model.

• Genomics & Informatics
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• 제6권1호
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• pp.18-28
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• 2008

Single nucleotide polymorphisms (SNPs) are the most abundant forms of human genetic variations and resources for mapping complex genetic traits and disease association studies. We have constructed a linkage disequilibrium (LD) map of chromosome 22 in Korean samples and compared it with those of other populations, including Yorubans in Ibadan, Nigeria (YRI), Centre d'Etude du Polymorphisme Humain (CEPH) reference families (CEU), Japanese in Tokyo (JPT) and Han Chinese in Beijing (CHB) in the HapMap database. We genotyped 4681 of 111,448 publicly available SNPs in 90 unrelated Koreans. Among genotyped SNPs, 4167 were polymorphic. Three hundred and five LD blocks were constructed to make up 18.6% (6.4 of 34.5 Mb) of chromosome 22 with 757 tagSNPs and 815 haplotypes (frequency $\geq$ 5.0%). Of 3430 common SNPs genotyped in all five populations, 514 were monomorphic in Koreans. The CHB + JPT samples have more than a 72% overlap with the monomorphic SNPs in Koreans, while the CEU + YRI samples have less than a 38% overlap. The patterns of hot spots and LD blocks were dispersed throughout chromosome 22, with some common blocks among populations, highly concordant between the three Asian samples. Analysis of the distribution of chimpanzee-derived allele frequency (DAF), a measure of genetic differentiation, Fst levels, and allele frequency difference (AFD) among Koreans and the HapMap samples showed a strong correlation between the Asians, while the CEU and YRI samples showed a very weak correlation with Korean samples. Relative distance as a quantitative measurement based upon DAF, Fst, and AFD indicated that all three Asian samples are very proximate, while CEU and YRI are significantly remote from the Asian samples. Comparative genome-wide LD studies provide useful information on the association studies of complex diseases.

• 한국지능시스템학회논문지
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• 제11권3호
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• pp.223-230
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• 2001

자기구성 지도는 주어진 입력에 대해 올바른 출력 값이 제공되지 않는 비교사 방식으로 학습된다. 또한, 반응하는 순서나 위치를 통해 위상이 보존(topology preserving)되는 특성을 가지고 있어 많은 분야에 응용되고 있다. 그러나, 자기 구성지도는 학습이 되기 전에 위상을 미리 고정시켜야 하기 때문에 실제 문제에 적용하기 어렵다는 단점을 가지고 있다. 구조 적응형 자기구성 지도는 자기구성 지도의 고정된 구조 때문에 발생하는 문제를 해결하기 위해 지도의 구조를 학습 중에 적절하게 변경시킨다. 이때, 변화된 구조의 가중치를 어떻게 초기화시킬 것인가 하는 것이 또한 중요한 문제이다. 이 논문에서는 구조 적응형 자기구성 지도 모델에서 유전자 알고리즘을 이용하여 분화된 노드의 가중치를 결정하는 방법을 제안한다. 이 방법은 기존의 구조 적응형 자기구성 지도보다 다소 높은 인식률을 보였고, 숫자 별 인식률 편차를 줄일 수 있었다. 오프라인 필기 숫자 데이터로 실험한 결과, 제안한 방법이 유용함을 알 수 있었다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.23-49
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• 1997

This is a progress report of rice biotechnology including development of gene transformation system, gene cloning and molecular mapping in rice. The scope of the research was focused on the connection between conventional breeding and biotech-researches. Plant transformation via Agrobacterium or particle bombardment was developed to introduce one or several genes to recommended rice cultivars. Two chimeric genes containing a maize ribosome inactivating protein gene (RIP) and a gerbicide resistant gene (bar) were introduced to Nipponbare, a Japonica cultivar, and transmitted to Korean cultivars. The homozygous progenies of herbicide resistant transgenic plant showed good fertility and agronomic characters. To explore the genetic resourses in rice, over 8,000 cDNA clones from immature rice seed have been isolated and sequenced. About 13% of clones were identified as enzymes related to metabolic pathway. Among them, twenty clones have high homology with genes encoding enzymes in the photorespiratory carbon cycle reaction. Up to now about 100 clones were fully sequenced and registered at EMBL and GenBank. For the mapping of quantitative tarits loci (QTL) and eternal recombinant inbred population with 164 F13 lines (MGRI) was developed from a cross between Milyang 23 and Gihobyeo, Korean rice cultivars. After construction of fully saturated RFLP and AFLP map, quantitative traits using MGRI population were analyzed and integrated into the molecular map. Eighty seven loci were determined with 27 QTL characters including yield and yield components on rice chromosomes. Map based cloning was also tried to isolate semi-dwarf (sd-1) gene in rice. A DNA probe, RG 109, the most tightly linked to sd-1 gene was used to screen from bacterial artifical chromosome (BAC) libraries and five over lapping clones presumably containing sd-1 gene were isolated. Rice genetic database including results of biotech reasearch and classical genetics is provided at Korea Rice Genome Server which is accessible with world wide web (www) browser. The server provides rice cDNA sequences and map informations linked with phenotypic images.