• Development and Reproduction
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• v.21 no.3
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• pp.269-274
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• 2017

The PCR analysis was performed on DNA samples extracted from a total of 20 individuals using six oligonucleotides primers. The author accomplished clustering analyses to reveal the Euclidean genetic distances among four clam populations from Gochang, Seocheon, Taean and Anmyeon of the Korean peninsula. The oligonucleotides primer OPA-08 generated 5 unique loci to each population, approximately 550 bp and 600 bp, respectively, in the MCS population. Especially, the primer OPA-20 generated 15 unique loci to each population, which were identifying each population, approximately 400 bp, 750 bp and 800 bp, in the MCT population. Individuals from MCG clam population ($0.637{\pm}0.227$) exhibited higher band-sharing values than did individuals from MCG clam population ($0.402{\pm}0.115$) (P<0.05). The dendrogram obtained by the six oligonucleotides primers indicates four genetic clusters: cluster 1 (MCG 01, 02, 04 and 05), cluster 2 (MCS 06, 07, 08, 09 and 10), cluster 3 (MCT 11, 12, 13, 14 and 15) and cluster 4 (MCA 16, 17, 18, 19, 20 and MCG 03). Among the twenty clam individuals, the shortest genetic distance that displayed significant molecular differences was between individuals 14 and 15 from the MCT population (genetic distance = 0.094), while the longest genetic distance among the twenty individuals that displayed significant molecular differences was between individuals MCG no. 01 and MCG no. 02 (genetic distance = 0.687). Comparatively, individuals of MCS clam population were fairly closely related to that of MCT clam population, as shown in the hierarchical dendrogram of Euclidean genetic distances.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1233-1237
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• 2008

The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively.

• Journal of Crop Science and Biotechnology
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• v.10 no.2
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• pp.106-111
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• 2007

Increasing genetic variability with mutagenic agents has been broadly employed in plant breeding because it has the potential to alter one or more desirable traits. In this study, a molecular analysis assessed by Amplified Fragment Length Polymorphisms(AFLPs) and a morphological analysis based on seedlings subjected to aluminum stress were compared. Also, an analysis of allelic frequencies was performed to observe unique alleles present in the pool. Genetic distances ranging from 0.448 to 0.953 were observed, suggesting that mutation inducing was effective in generating variability. The genetic distances based on morphological data ranged from 0(genotypes 22 and 23) to 30.38(genotypes 15 and 29). In the analysis of allelic frequency, 13 genotypes presented unique alleles, suggesting that mutation inducing was also targeting unique sites. Mutants with good performance under aluminum stress(9, 15, 18 and 27) did not form the same clusters when morphological and molecular analyses were compared, suggesting that different genomic regions may be responsible for their better performance.

• Development and Reproduction
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• v.17 no.4
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• pp.363-367
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• 2013

PCR analysis generated on the genetic data showed that the geographic hairtail (Trichiurus lepturus) population from Korea in the Yellow Sea was more or less separated from geographic hairtail population from China in the South Sea. The average bandsharing value ($mean{\pm}SD$) within hairtail population from Korea showed $0.859{\pm}0.031$, whereas $0.752{\pm}0.039$ within population from China. Also, bandsharing values between two hairtail populations ranged from 0.470 to 0.611, with an average of $0.542{\pm}0.059$. As compared separately, the bandsharing values of individuals within hairtail population from Korea were comparatively higher than those of individuals within population from China. The hierarchical dendrogram resulted from reliable oligonucleotides primers, indicating two genetic clusters composed of cluster 1 (KOREANHAIR1~KOREANHAIR11) and cluster 2 (CHINESEHAI12~CHINESEHAI22). The genetic distances between two geographic populations ranged from 0.038 to 0.476. Individual No. 11 within hairtail population from Korea was genetically closely related with No. 10 (genetic distance=0.038). The longest genetic distance (0.476) displaying significant molecular difference was also between individual No. 01 within hairtail population from Korea and No. 22 from Chinese. In the present study, PCR analysis has revealed significant genetic distances between two hairtail population pairs (P<0.05).

• Animal Systematics, Evolution and Diversity
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• v.36 no.3
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• pp.258-263
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• 2020

The aim of this study is to report monotypic species, Nonparahalosydna pleiolepis(Marenzeller, 1879) for the first time from Korean waters with its DNA barcoding data. We collected individuals of the species from the subtidal zone of southern coast of Korea through scuba diving. To estimate DNA barcoding gap, the pairwise genetic distances were calculated between N. pleiolepis and its congeners (Halosydna brevisetosa Kinberg, 1856 and Lepidonotus squamatus (Linnaeus, 1758)) based on the cytochrome c oxidase subunit I gene (COI). Inter-specific genetic distances ranged from 18.7% to 24.6%, while intra-specific genetic distance within N. pleiolepis ranged from 0.3% to 0.5%. The maximum intra-specific genetic distance among the three species was 1.4%. The morphological diagnosis of N. pleiolepis with a taxonomic note on the species were also provided.

• Development and Reproduction
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• v.25 no.2
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• pp.105-111
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• 2021

A PCR-founded genetic analysis aim and principle was used to foster a hierarchical polar dendrogram of the Euclidean genetic distances (GDs) for two arkshell populations, Scapharca broughtonii (YEOSU, Yeosu population and JINHAE, Jinhae population). Five oligonucleotides primers were make use of to craft 354 and 390 scorable bands in the Yeosu and Jinhae populations, respectively, outspreading in DNA fragment size from 100 bp to 1,600 bp. The bandsharing (BS) results disclosed that the Jinhae population had a higher average BS value (0.700) than that for the Yeosu population (0.692). The GD between individuals supported an adjacent association in grouping II (JINHAE 12 - JINHAE 22). The observation of a noteworthy GD between the two Scapharca populations verified that this PCR-generated technique could be a profitable attempt for within- and between-population-grounded biological DNA scrutiny. The potential of PCR inquiry will be favorable in the selection of individuals and/or populations for several reproductive- and/or quarantine-connected characters in aquafarming manufacture.

• Development and Reproduction
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• v.18 no.2
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• pp.89-98
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• 2014

The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05).

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.27-32
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• 2008

The objective of this study was to estimate genetic trend on racing time of Thoroughbred racehorses in Korea, using a total of 209,725 racing records of 9,934 racehorses collection from January, 1990 to December, 2006. Phenotypic trends for all distances were negative at a rate of －0.148, －0.137, －0.137 and －0.139 second per race year for distances of 1,000m, 1,400m less than, 1,700m more than and overall dataset, respectively. Environmental trends were similar to phenotypic ones in all distances and trends in permanent environmental and jockey effects by race year were not found. Average genetic improvements for racing time were －0.037 and －0.030 second per race year at the 1,000m and overall dataset, respectively, which is low. But Genetic trends were decreased consistently. There is need to establish the genetic improvement program for quality of racehorses.

• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.733-740
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• 2008

The purpose of this study was to assess the genetic variation and establish the relationship amongst Hanwoo, Angus and Holstein breed. The genetic characteristics and variability within Hanwoo(300), Angus(80) and Holstein(50) were estimated on the basis of relationships determined using the 10 kinds of microsatellite, which is located on different chromosomes. Frequencies of microsatellites markers were used to estimate heterozygosities, polymorphic information content(PIC) and genetic distances. The PICs ranged from 0.604 to 0.872(Hanwoo), from 0.562 to 0.812(Angus) and from 0.471 to 0.828(Holstein). Observed heterozygosity and PIC of Hanwoo are the highest among the analyzed breeds. Additionally, Estimates of genetic distance can be utilized to identify genetic relationships between Hanwoo and the other breed. Genetic distances(0.233) between Hanwoo and Angus was lower than distances between Hanwoo and Holstein(2.283). Also, Genetic distances between Angus and Holstein was shown for(2,400). The other side, each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all the populations.

• Journal of Sericultural and Entomological Science
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• v.30 no.1
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• pp.8-19
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• 1988

The objective of the present study was to investigate the behavioral responses of sillkworm (Bombyx mori) larvae to: the dominant effect of walking distace: and the relationships between walking distance and the various economic characters on yellow sheets. The means of walking distances of progeny from F2 and F1 F1 and F2 generations, which was a hybrid between the long and the short walking distances, were shorter than those of parents. This may indicate that the genes for the short walking distances were partially dominant over those for the longwalking. Estimated broad sense heritability of larval walking distance was different for each larval instar, which fell into the range of 52∼84%. Genetic correlation was apparent between walking distances of all larval stages and some economic characters. The number of cocoons per liter and the period from incubation to emergence have shown the positive genetic correlation with walking distances. The cocoon yield, the sing cocoon weight, and the cocoon shell weight have shown to be in the negative correlation with walking distances. No correlation was observed between walking distances of the mature larvae and any economic characters. The analysis by the path coefficient showed negative direct effect of walking distances on the cocoon yield, and positive indirect effect on the single cocoon weight and the cocoon shell weight.