• Title/Summary/Keyword: Genetic Architecture

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Site Environment and Morphology Characteristics of Native Bermudagrass (Cynodon spp.) Ecotypes in Korea (국내에서 수집된 버뮤다그래스의 입자환경 및 형태적 특성)

  • Bae, Eun-Ji;Lee, Kwang-Su;Park, Nam-Chang;Lee, Sang-Myung;Shin, Hyun-Cheol;Yang, Geun-Mo
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.11-16
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    • 2011
  • This study was conducted to identify the morphological characteristics and variations level by site environment of native bermudagrass (Cynodon spp.) from costal and island region in South Korea. Soil chemical properties and morphological characteristics were investigated. There were significant differences in plant height, leaf width, leaf length, cotton on leaf blade, stolon, and number of seed per spike depending on where they were collected from natural habitat. We have discovered a variety of variation among the investigated traits in Korean native bermudagrass ecotypes. There were broad leaf and fine leaf types of native bermudgarass in S. Korea. Adaptability was in very high on inadequate environment in bermudagrass with broad leaf types, it has also been suggested that management worth in terms of use. These results may provide basic information for bermudagrass breeding development and the collected types during this investigation would be worth being preserved as genetic resources for further breeding purposes.

Stabilization Controller Design of a Container Crane for High Productivity in Cargo Handling Using a RCGA (실수코딩유전알고리즘을 이용한 하역생산성 향상용 컨테이너 크레인의 안정화 제어기 설계)

  • Lee, Soo-Young;Ahn, Jong-Kap;Choi, Jae-Jun;Son, Jeong-Ki;Lee, Yun-Hyung;So, Myung-Ok
    • Journal of Navigation and Port Research
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    • v.31 no.6
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    • pp.515-521
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    • 2007
  • To increase the stevedore efficiency and service level at container terminal, it is essential to reduce working time of container crane which has a bottle neck in the logistic flow of container. The working speed and safety are required to be improved by controlling the movement of the trolley as quick as possible without big overshoot and any residual swing motion of container in the vicinity of target position. This paper presents optimal state feedback control using RCGAs in the case of existing constrained conditions

Studies on the Effects of Deleterious Genes on the Strains Selected for Photoaxis in Drosophila melanogaster (초파리에 있어서 주광성행동의 선발에 관한 유해유전자의 영향)

  • Jong-Kil Choo
    • The Korean Journal of Zoology
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    • v.18 no.1
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    • pp.1-8
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    • 1975
  • The experiment has been analyzed for genetic architecture of behavioral trait in strains selected for positive and negative phototaxis in Drosophila melanogaster. Response to selection for two different directions was rapidly diverged in their phototactic scores. Realized heritabilities for the first teh generations of selection in the positive and negative strains calculated to be 3.08% and 2.86%, respectively in both sexes. The frequency of deleterious chromosomes(lethal and semilethal chromosomes) in the positively selected strain(43%) was higher than that of negatively selected one (18%). The unselected strain(27%) was intermediate frequency between both selected strains. The correlated relationship between deleterious genes and photopositive polygenes was confirmed by the phototactic behavior of the lethal heterozygotes($1_i/1_j$). Form the results, the deleterious genes induced by long term selection for phototaxis would be considered some linkage relationship with photopositive polygenes.

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Quantitative Trait Loci Mapping for Porcine Backfat Thickness

  • Wu, X.L.;Lee, C.;Jiang, J.;Peng, Y.L.;Yan, H.F.;Yang, S.L.;Xiao, B.N.;Liu, X.C.;Shi, Q.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.932-937
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    • 2002
  • A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.

A Comparative Genome-Wide Analysis of GATA Transcription Factors in Fungi

  • Park, Jong-Sun;Kim, Hyo-Jeong;Kim, Soon-Ok;Kong, Sung-Hyung;Park, Jae-Jin;Kim, Se-Ryun;Han, Hyea-Young;Park, Bong-Soo;Jung, Kyong-Yong;Lee, Yong-Hwan
    • Genomics & Informatics
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    • v.4 no.4
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    • pp.147-160
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    • 2006
  • GATA transcription factors are widespread eukaryotic regulators whose DNA-binding domain is a class IV zinc finger motif in the form $CX_{2}CX_{17-20}CX_{2}C$followed by a basic region. In fungi, they act as transcriptional activators or repressors in several different processes, ranging from nitrogen source utilization to mating-type switching. Using an in-house bioinformatics portal system, we surveyed 50 fungal and 9 out-group genomes and identified 396 putative fungal GATA transcription factors. The proportion of GATA transcription factors within a genome varied among taxonomic lineages. Subsequent analyses of phylogenetic relationships among the fungal GATA transcription factors, as well as a study of their domain architecture and gene structure, demonstrated high degrees of conservation in type IVa and type IVb zinc finger motifs and the existence of distinctive clusters at least at the level of subphylum. The SFH1 subgroup with a 20-residue loop was newly identified, in addition to six well-defined subgroups in the subphylum Pezizomycotina. Furthermore, a novel GATA motif with a 2f-residue loop ($CX_{2}CX_{21}CX_{2}C$, designated 'zinc finger type IVc') was discovered within the phylum Basidiomycota. Our results suggest that fungal GATA factors might have undergone multiple distinct modes of evolution resulting in diversified cellular modulation in fungi.

Agent-based System for Complex Decision Making and Negotiation: Application to Virtual Manufacturing (복잡한 의사결정과 협상 환경을 위한 에이전트 기반 시스템: 가상생산 응용)

  • Lee, Kyoung-Jun;Chang, Yong-Sik;Choi, Hyung-Rim;Kim, Hyun-Soo;Park, Young-Jae;Park, Byung-Joo
    • Information Systems Review
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    • v.4 no.2
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    • pp.223-236
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    • 2002
  • In an agent-based system, each agent has its own decision making capability and competes, cooperates, and communicates each other with an agent interface. Virtual manufacturing has characteristics as a typical application area of agent-based system: complex and time-bound decision making and negotiation. The time-boundness influences the choice of decision making models and design of protocols for internal and external negotiation. In this paper, we provide a case study which suggests a time-bound framework for external and internal negotiation between the agents for virtual manufacturing environment. We illustrate decision making model selection strategy and the system architecture of the agent-based system for the complex and time-bound environment.

A Study on the Composition and Operating Trend of Arboreta in Korea (국내수목원 조성 및 운영경향에 관한 연구)

  • Kim, Jong Ik;Kang, Cheol Gi;Shin, Hyun Tak;Seng, Jung Won
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.14 no.5
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    • pp.37-51
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    • 2011
  • Research and conservation of plant species for the nation arboretum, collection and securing of the plant species for both public and private arboreta and education for the arboreta which is affiliated to the university are overall management goal of the arboreta in Korea. Most of the arboreta in Korea run the programs and events for guide and interpretation of forest, but the programs and events are not well specified by the arboreta. Regarding the reasonable level of entrance fee should be discussed further as the lack of information and understanding. But the specified entrance fee between public and private arboreta should be considered. An eighty three percent of arboreta in Korea has been properly managed the species information. The reasonable management should be considered in case of unusual management of collected plant genetic resources. Although the overall impending difficulties for the management of arboreta in Korea is the lack of trained or qualified staffs, securing tasks independently for the public arboreta and relatively low salary for the private arboreta are main difficulties. Most of the difficulties are derived from the lack of its mission of the arboreta. The management of arboreta by the mission statement is one of the core targets including the characters such as climate or region should be considered. The securing qualified or trained staffs should be considered in the development of arboreta in Korea including the modification of relevant regulations or law.

Identification and Functional Analysis of SEDL-binding and Homologue Proteins by Immobilized GST Fusion and Motif Based Methods

  • Hong, Ji-Man;Jeong, Mi-Suk;Kim, Jae-Ho;Kim, Boog-il;Holbrook, Stephen R.;Jang, Se-Bok
    • Bulletin of the Korean Chemical Society
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    • v.29 no.2
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    • pp.381-388
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    • 2008
  • An X-linked skeletal disorder, SEDT (spondyloepiphyseal dysplasia tarda) is a genetic disease characterized by a disproportionately short trunk and short stature caused by mutations in the SEDL gene. This gene is evolutionarily conserved from yeast to human. The yeast SEDL protein ortholog, Trs20p, has been isolated as a member of a large multi-protein complex called the transport protein particle (TRAPP), which is involved in endoplasmic reticulum (ER)-to-Golgi transport. The interaction between SEDL and partner proteins is important in order to understand the molecular mechanism of SEDL functions. We isolated several SEDL-binding proteins derived from rat cells by an immobilized GST-fusion method. Furthermore, the SEDL-homologue proteins were identified using motif based methods. Common motifs between SEDL-binding proteins and SEDL-homologue proteins were classified into seven types and 78 common motifs were revealed. Sequence similarities were contracted to seven types using phylogenetic trees. In general, types I-III and VI were classified as having the function of acetyl-CoA carboxylase, glycogen phosphorylase, isocitrate dehydrogenase, and enolase, respectively, and type IV was found to be functionally related to the GST protein. Types V and VII were found to contribute to TRAPP vesicle trafficking.

A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology

  • Zaky, Abdelrahman Saleh;Greetham, Darren;Louis, Edward J.;Tucker, Greg A.;Du, Chenyu
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1891-1907
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    • 2016
  • Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from marine environments could have interesting characteristics for industrial applications. However, the application of marine yeast in research or industry is currently very limited owing to the lack of a suitable isolation method. Current methods for isolation suffer from fungal interference and/or low number of yeast isolates. In this paper, an efficient and non-laborious isolation method has been developed and successfully isolated large numbers of yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA. These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose, mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC 2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved sugar utilization using seawater-based media was confirmed and, therefore, they could potentially be utilized in fermentations using marine biomass in seawater media, particularly for the production of bioethanol and other biochemical products.

Understanding Epistatic Interactions between Genes Targeted by Non-coding Regulatory Elements in Complex Diseases

  • Sung, Min Kyung;Bang, Hyoeun;Choi, Jung Kyoon
    • Genomics & Informatics
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    • v.12 no.4
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    • pp.181-186
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    • 2014
  • Genome-wide association studies have proven the highly polygenic architecture of complex diseases or traits; therefore, single-locus-based methods are usually unable to detect all involved loci, especially when individual loci exert small effects. Moreover, the majority of associated single-nucleotide polymorphisms resides in non-coding regions, making it difficult to understand their phenotypic contribution. In this work, we studied epistatic interactions associated with three common diseases using Korea Association Resource (KARE) data: type 2 diabetes mellitus (DM), hypertension (HT), and coronary artery disease (CAD). We showed that epistatic single-nucleotide polymorphisms (SNPs) were enriched in enhancers, as well as in DNase I footprints (the Encyclopedia of DNA Elements [ENCODE] Project Consortium 2012), which suggested that the disruption of the regulatory regions where transcription factors bind may be involved in the disease mechanism. Accordingly, to identify the genes affected by the SNPs, we employed whole-genome multiple-cell-type enhancer data which discovered using DNase I profiles and Cap Analysis Gene Expression (CAGE). Assigned genes were significantly enriched in known disease associated gene sets, which were explored based on the literature, suggesting that this approach is useful for detecting relevant affected genes. In our knowledge-based epistatic network, the three diseases share many associated genes and are also closely related with each other through many epistatic interactions. These findings elucidate the genetic basis of the close relationship between DM, HT, and CAD.