• Molecules and Cells
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• v.40 no.11
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• pp.828-836
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• 2017

Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

• Journal of Power System Engineering
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• v.20 no.6
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• pp.27-33
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• 2016

BLDC motors have the strong point of allowing high-efficiency operation, yet with the weak point of requiring position information for operation. Moreover, there have recently been frequent cases where the product differentiation of even a cheap BLDC motor demanded a high level of control performance similar to that of a controller using a high-precision position sensor. This paper proposes low cost position sensor that enables the acquisition of rotor position information based on index and incremental PWM, using a single position sensor instead of an expensive incremental encoder or a cheap 3 phase hall sensor. The characteristic of the proposed encoder is that index information at every $60^{\circ}$ of electrical angle is inscribed on the encoder disk, as well as a multiple number of values representing information about PWM, which is obtained by modulating information about the electrical angle of the rotor that has the resolution of $60^{\circ}$. Such a method has the characteristic of enabling the acquisition of high-precision position information based on the information about a multiple number of PWM waveforms and counter values that have all been inscribed on the encoder disk for each $60^{\circ}$ range. The feasibility of the proposed new encoder was verified by fabricating a prototype encoder generating 240 pulses, followed by confirming its performance using Micom's capture and software counter functions.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.31 no.3
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• pp.235-256
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• 2020

The purpose of this study is to define a method of building an integrated service to provide various science and technology knowledge infrastructures that are helpful in R&D activities, and to show the cases that are adapted the methodologies. Knowledge infrastructures scattered throughout the entire R&D cycle, such as generating/development of ideas, finding the R&D project, performing the project, and spreading results, are segmented in terms of services, functions, information, and data, and links and converges to provide the knowledge infrastructure that desired by users in one place. We define the integrated service classification, integration level model, integrated architecture, and integrated user authentication system in consideration of logical linkage and integration rather than physical integration of individual knowledge infrastructures. Also, we considered the extensibility as the reference model for building of similar integrated service.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.5
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• pp.970-976
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• 2001

In this paper, OUIG IDL CFE, provided by Sunsoft, is used to take a IDL definitions as inputs and parse those. OmniORB3 is introduced to support functionality of the ORB. Suns CFE produce AST after parsing inputs. Actually, the node of AST Is instances of classes which are derived from CFE classes. As the compiler back end visit the node of the AST using iterator class, UTL_ScopeActiveIterator, it dumps codes of output. During processing, two files are generated. Routines of generating code are invoked by BE_produce.cc and codes are produced while visiting root of AST, idl_global->root(). The dump* functions which dump codes is called according to the type of node. In this paper, Mapping C++ of IDL definition is experimented and results In the same as that of omniidl which is provided by omniORB3. The code of results behavior correctly on omniORB3. In the future, we are interested in optimizing the performance of marshalling code via IDL compiler.

• Journal of KIISE:Databases
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• v.29 no.6
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• pp.417-428
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• 2002

XML(extensible Markup Language) is effective to information retrieval and sharing but has defects related to the data security. And, as a solution of this problem, the current XML security researches such as XML digital signature, XML data encryption, and XML access control exclude the validation property of XML document. The validation of XML should be considered for the secure information sharing in the XML-based environment. In this paper, we design and implement the system to support both security and validation to XML document. Our system performs data encryption and maintenance of valid status of XML document by referencing new XML schema namespace. In addition, it also provides the XML schema security function through the XML schema digital signature. During generating XML schema digital signature, DOMHash method which has the advantage of the faster speed than canonical XML method is applied to XML schema. In conclusion, our system shows the improved functions in flexibility, scalability, and reliability compared with the existing XML security researches.

• The KIPS Transactions:PartD
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• v.17D no.5
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• pp.359-370
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• 2010

As web 2.0 becomes one of the important architecture styles, more web applications adopt single page structure instead of multiple web pages and navigations between pages. A single page web application client, called a mashup client in this paper, interfaces more than one services and allows users to navigate in the page. A mashup client page includes complicated functions and has to handle various styles of services and user requirements, and therefore is usually developed manually. In this paper, we propose a model driven code generation approach for in-page navigations. We propose a page model and view navigation design approach, applying REST service architecture patterns. Then, we consider type conditions for each view to have service calls or navigation controls. Also, we developed an XForms page code generation system to demonstrate the efficiency of the proposed method. The developed system generates mashup client pages including navigation controls between services and views. This system can generate ready to use codes from service specifications, so this can help to reduce the development overhead. Moreover, our approach is based on formal model and navigation patterns so the generated result code is simple and easy to understand, and includes only the necessary controls. Therefore, the proposed approach can be more effective for the case of a large number of services.

• Journal of Biomedical Engineering Research
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• v.26 no.4
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• pp.223-230
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• 2005

This paper presents a new approach to investigate spatial correlation between independent components of brain alpha activity in functional magnetic resonance imaging (fMRI) and electroencephalography (EEG). To avoid potential problems of simultaneous fMRI and EEG acquisitions in imaging pure alpha activity, data from each modality were acquired separately under a 'three conditions' setup where one of the conditions involved closing eyes and relaxing, thus making it conducive to generation of alpha activity. The other two conditions -- eyes open in a lighted room or engaged in a mental arithmetic task, were designed to attenuate alpha activity. Using a Mixture Density Independent Component Analysis (MD-ICA) that incorporates flexible non-linearity functions into the conventional ICA framework, we could identify the spatiotemporal components of fMRI activations and EEG activities associated with the alpha rhythm. Then, the sources of the individual EEG alpha activity component were localized by a Maximum Entropy (ME) method that is specially designed to find the most probable dipole distribution minimizing the localization error in sense of LMSE. The resulting active dipoles were spatially transformed to 3D MRls of the subject and compared to fMRI alpha activity maps. A good spatial correlation was found in the spatial distribution of alpha sources derived independently from fMRI and EEG, suggesting the proposed method can localize the cortical areas responsible for generating alpha activity successfully in either fMRI or EEG. Finally a functional connectivity analysis was applied to show that alpha activity sources of both modalities were also functionally connected to each other, implying that they are involved in performing a common function: 'the generation of alpha rhythms'.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.2
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• pp.27-38
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• 2003

This study aimed to provide basic data for future management of roadside trees by analyzing problems associated with plantation and management of roadside trees through categorization of functions and organizational structure, plantation and management, and management regulations and generating improvement opportunities based on the result of a survey on the awareness of government officials who are directly in charge of managing roadside trees in 31 cities & counties within Kyonggi province. The summary of key results of this study is as follows. First, "roadside tree-related function" is still not considered to be independent. About a half of government officials in charge did not majored in this field and they spent only about "2.09hours", in average, in the roadside tree-related function out of 8 working hours per day. Second, regarding problems and improvement opportunities in roadside tree-related function, lack of time was considered to be the biggest problem in overall management of roadside trees. As for improvement opportunities, "increase of dedicated manpower" and "system development" to facilitate efficient processing were suggested. Third, an annual budget for roadside tree-related affairs was merely 92 million won per city/county. A registry for management of roadside trees was kept manually. As for the roles of roadside trees, improvement of landscape and securing of green area itself were valued highly. Fourth, Ginkgo biloba, Zelkova serrata, and Prunus yedoensis were suggested to be the most appropriate species for roadside tree and Platanus occidentalis, Populus albaglandulosa, Populus euramericana, and Robinia pseudo-acacia were mentioned to be the least appropriate species.

• Communications for Statistical Applications and Methods
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• v.19 no.2
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• pp.257-266
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• 2012

R is a free software for statistical analysis that provides simple interfaces to other application programs. Many people are trying to learn R, but it is difficult to learn R compared to commercial software such as SPSS or SAS, and it is cumbersome to provide an environment to teach R. Thus, it is essential to provide a new web-based R environment for novice users or for laboratory use. We developedWebER (a web-based R environment) using PHP on the Linux apache server. WebER can be easily used by any R user because we implemented the same functions as the basic Rgui such as editing R program, generating the text, image outputs, errors and warnings. It is also possible for multi-users to access WebER.

• Proceedings of the KSRS Conference
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• 1998.09a
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• pp.313-318
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• 1998

Electro-Optical Camera(EOC) is the main payload of Korea Multi-Purpose SATellite(KOMPSAT) with the mission of cartography to build up a digital map of Korean territory including Digital Terrain Elevation Map(DTEM). This instrument which comprises EOC Sensor Assembly and EOC Electronics Assembly produces the panchromatic images of 6.6 m GSD with a swath wider than 17 km by push-broom scanning and spacecraft body pointing in a visible range of wavelength, 510 ~ 730 nm. The high resolution panchromatic image is to be collected for 2 minutes during 98 minutes of orbit cycle covering about 800 km along ground track, over the mission lifetime of 3 years with the functions of programmable rain/offset and on-board image data storage. The image of 8 bit digitization, which is collected by a full reflective type F8.3 triplet without obscuration, is to be transmitted to Ground Station at a rate less than 25 Mbps. EOC was elaborated to have the performance which meets or surpasses its requirements of design phase. The spectral response the modulation transfer function, and the uniformity of all the 2592 pixel of CCD of EOC are illustrated as they were measured for the convenience of end-user. The spectral response was measured with respect to each gain setup of EOC and this is expected to give the capability of generating more accurate panchromatic image to the EOC data users. The modulation transfer function of EOC was measured as greater than 16% at Nyquist frequency over the entire field of view which exceeds its requirement of larger than 10%, The uniformity that shows the relative response of each pixel of CCD was measured at every pixel of the Focal Plane Array of EOC and is illustrated for the data processing.