• 제목/요약/키워드: Gene isolation

검색결과 863건 처리시간 0.033초

A study on the establishment of isolation distances for environmental release of biotech crops

  • Lee, Bumkyu
    • 농업과학연구
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    • 제44권2호
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    • pp.188-195
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    • 2017
  • Biotech crops can only be commercialized after they receive safety approvals, which require thorough risk assessments of their release to the environment. Environmental release experiments are indispensable for environmental risk assessments, and each country has been preparing its own regulations for the safety management of experiments on the environmental release of biotech crops in confined fields. In this study, we compared and analyzed the safety management regulations of the environmental release of biotech crops in Korea, USA, Japan, European Union, and China. Each country had safety management regulations for the environmental release of biotech crops, and these regulations were generally not much different from the Korean regulations. However, there was a difference amongst the USA, Japan, and China in regulations for isolation distances to prevent gene diffusion through pollen-flow during environmental release experiments of biotech crops. In order to establish the isolation distance regulation suitable for the Korean environment, relevant data were collected and presented. For setting the isolation distance for environment release of biotech crops, it is suggested to refer to the isolation distance information provided in the Guidance of Seed Management in Korea. The results of this study are expected to help establish the safety management of biotech crops in Korea.

E. coli pRDI에서의 DAP-영양요구성 변이주 분리 및 동정 (Isolation and Identifition of DAP-Auxotrophs from E. coli pRDI)

  • 이호자
    • 미생물학회지
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    • 제22권4호
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    • pp.265-269
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    • 1984
  • E. coli $P^{RDI}$에서 DAP-영양요구성 변이주를 분리하였으며 이 변이주가 갖고 있는 plasmid의 안정성과 활성도를 확인하는 살험을 통하여 다음과 같은 결론을 얻었다. 1. Mutagen인 NG를 처리 후 DAP-변이주를 보다 쉽게 분리하기 위하여 항생제처리를 하였다. 이때 사용균주가 갖고 있는 plasmid내의 내성인자들 중에 carbr인자로 인하여 같은 계열에 있는 penicillin유도체들에 대해서는 교차내성을 갖고 있음이 확인되었다. 그러냐 penicillin과 같은 기능을 갖고 있으나 그 구조가 다른 cephalexin, cycloserine에 대해서는 교차 내성을 잘 나타내지 않았으므로 항생제처리로서는 cephalexin을 사용하였다. 2. 세균 접합을 통하여 DAP-균주의 특성을 동정하였다. 즉 nif-gene의 안정성과 활성도는 DAP-균주로 부터 plasmid를 전이반은 전이체에서 6-cyanopurine첨가로 확인하였다.

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Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea

  • Lee, Jae-Yun;Jeong, Yun-Seok;Kim, Pil Soo;Hyun, Dong-Wook;Bae, Jin-Woo
    • Journal of Species Research
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    • 제10권1호
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    • pp.1-11
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    • 2021
  • Evidence highlighting the importance of gut microbiota in biodiversity conservation is growing; however, gut bacteria in South Korean wildlife have not been well identified. Using a culture-dependent isolation method, we identified the gut bacteria from Korean aquatic wildlife: the gazami crab (Portunus trituberculatus), Korean striped bitterling (Acheilognathus yamatsutae), oily bitterling (Acheilognathus koreensis), leopard mandarin fish (Siniperca scherzeri), Korean dark chub (Zacco koreanus), diving beetle (Cybister lewisianus), spotted steed (Abbottina springeri), and Korean spotted sleeper (Odontobutis obscura interrupta). We identified 18 strains previously unrecorded in South Korea by comparing 16S rRNA gene sequences of isolates against the EzBioCloud and National Institute of Biological Resources(NIBR) databases. The isolated strains belong to the phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. We also assessed for phylogenetic relatedness, Gram-stain reaction, colony and cell morphology, and biochemical characteristics. Basic information and 16S rRNA gene sequences of the isolates were registered in NIBR, and NIBR accession numbers are provided.