• Title/Summary/Keyword: Gene isolation

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Isolation and Characterization of a Putative SENESCENCE 1 Gene from Poplar (Populus alba × P. glandulosa) (현사시나무에서 SENESCENCE 1 유전자의 분리와 발현특성 구명)

  • Kim, Joon-Hyeok;Lee, Hyoshin;Choi, Young-Im;Bae, Eun-Kyung;Yoon, Seo-Kyung;Noh, Seol Ah
    • Korean Journal of Plant Resources
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    • v.27 no.4
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    • pp.392-399
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    • 2014

  • Plant senescence is one of the survival strategies to use limited nutrients efficiently during growth, development and adaptation. In this study, we isolated a gene (PagSEN1) homologous to SENESCENCE 1 from Populus alba ${\times}$ P. glandulosa. The PagSEN1 gene encodes a putative protein consisting of 243 amino acids containing a rhodanese domain. Southern blot analysis suggested that two copies of the PagSEN1 gene are present in the poplar genome. We characterized its transcriptional expression under various conditions mimicking senescence and environmental stresses. The PagSEN1 was expressed most strongly in mature leaves but most weakly in roots. The gene was significantly up-regulated by treatments with mannitol, NaCl, ABA and JA, but not by cold, SA and GA3. These results indicate that PagSEN1 is involved in senescence response induced by environmental stresses.

  • https://doi.org/10.7732/kjpr.2014.27.4.392 인용 PDF KSCI KPUBS HTML