• Parasites, Hosts and Diseases
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• v.58 no.4
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• pp.431-443
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• 2020

Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) is described based on adult flukes collected from humans residing along the Mekong River in Cambodia. Total 256 flukes were collected from the diarrheic stool of 6 echinostome egg positive villagers in Kratie and Takeo Province after praziquantel treatment and purging. Adults of the new species were 9.0-13.1 (av. 11.3) mm in length and 1.3-2.5 (1.9) mm in maximum width and characterized by having a head collar armed with 37 collar spines (dorsal spines arranged in 2 alternative rows), including 5 end group spines. The eggs in feces and worm uterus were 98-132 (117) ㎛ long and 62-90 (75) ㎛ wide. These morphological features closely resembled those of Echinostoma revolutum, E. miyagawai, and several other 37-collar-spined Echinostoma species. However, sequencing of the nuclear ITS (ITS1-5.8S rRNA-ITS2) and 2 mitochondrial genes, cox1 and nad1, revealed unique features distinct from E. revolutum and also from other 37-collar-spined Echinostoma group available in GenBank (E. bolschewense, E. caproni, E. cinetorchis, E. deserticum, E. miyagawai, E. nasincovae, E. novaezealandense, E. paraensei, E. paraulum, E. robustum, E. trivolvis, and Echinostoma sp. IG). Thus, we assigned our flukes as a new species, E. mekongi. The new species revealed marked variation in the morphology of testes (globular or lobulated), and smaller head collar, collar spines, oral and ventral suckers, and cirrus sac compared to E. revolutum and E. miyagawai. Epidemiological studies regarding the geographical distribution and its life history, including the source of human infections, remain to be performed.

• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.216-225
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• 2020

An esterase gene, estA1, was cloned from Alteromonas sp. 39-G1 isolated from the Beaufort Sea. The gene is composed of 1,140 nucleotides and codes for a 41,190 Da protein containing 379 amino acids. As a result of a BLAST search, the protein sequence of esterase EstA1 was found to be identical to Alteromonas sp. esterase (GenBank: PHS53692). As far as we know, no research on this enzyme has yet been conducted. Phylogenetic analysis showed that esterase EstA1 was a member of the bacterial lipolytic enzyme family IV (hormone sensitive lipases). Two deletion mutants (Δ20 and Δ54) of the esterase EstA1 were produced in Escherichia coli BL21 (DE3) cells with part of the N-terminal of the protein removed and His-tag attached to the C-terminal. These enzymes exhibited the highest activity toward p-nitrophenyl (pNP) acetate (C₂) and had little or no activity towards pNP-esters with acyl chains longer than C₆. Their optimum temperature and pH of the catalytic activity were 45℃ and pH 8.0, respectively. As the NaCl concentration increased, their enzyme activities continued to increase and the highest enzyme activities were measured in 5 M NaCl. These enzymes were found to be stable for up to 8 h in the concentration of 3-5 M NaCl. Moreover, they have been found to be stable for various metal ions, detergents and organic solvents. These salt-tolerant and chemical-resistant properties suggest that the enzyme esterase EstA1 is both academically and industrially useful.

• Korean Journal of Microbiology
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• v.53 no.2
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• pp.141-143
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• 2017

Caballeronia sordidicola strain PAMC 26577 was isolated from Cladonia sp., a lichen collected from Svalbard Archipelago in the Arctic Ocean. Draft genomic sequences of PAMC 26577 were determined using Illumina and 182 contigs were submitted to GenBank and N50 value was 159,226. The genome of PAMC 26577 was comprised of 8,334,211 base pairs and %G+C content was 59.4. The genome included 8 ribosomal RNA genes and 51 tRNA genes as non-coding sequences. Protein-coding genes were 8,065 in number and they included central metabolism genes as well as butanol/butyrate biosynthesis, polyhydroxybutyrate metabolism, serine cycle methylotrophy genes, and glycogen metabolism. Membrane transporters were more than two-hundreds in number, but sugar phosphotransferase system and TRAP transporters were lacking. PAMC 26577 lacked CRISPR-associated sequences and proteins. No transposable elements were observed and there were only limited number of phage remnant regions with 11 phage-related genes.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.332-338
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• 2009

A xylan-decomposing bacterium, HY-20, was isolated from the gut of a honeybee, Apis mellifera, and identified as Bacillus sp. The extracellular GH11 xylanase (XylP) gene (687-bp) of strain HY-20 encoded a protein of 228 amino acids with a deduced molecular mass of 25,522 Da and a calculated pI of 9.33. The primary structure of XylP was 97% identical to that of B. pumilus xylanase (GenBank accession no.: AY526092) that has not been characterized yet. The recombinant His-tagged enzyme (rXylP) overexpressed in Escherichia coli BL21 harboring pET-28a(+)/xylP was purified to electrophoretic homogeneity by cation exchange and gel permeation chromatographies. The purified enzyme exhibited the highest catalytic activity toward birchwood xylan at pH 6.5 and $50^{\circ}C$ and retained approximately 50% of its original activity when pre-incubated at $55^{\circ}C$ for 15 min. The recombinant enzyme was completely inactivated by $Hg^{2+}$ (1 mM) and N-bromosuccinimide (5 mM), while its activity was slightly stimulated by approximately 10% in the presence of $Mn^{2+}$ (1 mM), $Fe^{2+}$ (1 mM), and sodium azide (5 mM). rXylP was able to efficiently degrade various polymeric xylose-based substrates but PNP-sugar derivatives and glucose-based polymers were not susceptible to the enzyme.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.5
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• pp.375-398
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• 2002

Um von der 'kritischen' Situation des Faches DaF rauszukommen, werden viele Versuche untemommen, durch die Verbesserung des Untenichts in der Richtung nach der Entwicklung der kommunikativen $F\"{a}higkeit$ Deutsch als Fremdsprache wieder attraktiv und $konkurrenzf\"{a}hig$ zu machen. Aber leider sieht die Zwischenbilanz nicht so erfolgreich aus. Eine der Ursachen $daf\"{u}r\;l\"{a}sst$ sich mE. darin sehen, dass die $Ans\"{a}tze,\;die\;haupts\"{a}chlich\;f\"{u}r$ den Unterricht von Deutsch als Zweitsprache entwickelt wurden, ohne $gen\"{u}gende$ Berucksichtigung auf die kulturellen und institutionellen Unterschiede zwischen den Lemsituationen im zielsprachigen Land und im Heimatland der Schuler eingesetzt werden. In diesem Kontext $w\"{a}re\;es\;n\"{o}tig$, auf die Unterschiede zwischen DaF und DaZ und ihre Wirkung auf die Lehrstrategien im DaF-Unterricht zuruckzugreifen, Dabei sind auf einige Faktoren besonders zu achten, die DaF-Untenicht vom DaZ-Untenicht unterscheiden: die Mangel an den $M\"{o}glichkeiten$ der authentischen Kommunikation, die $Homogenit\"{a}t\;der\;Sch\"{u}ler$, die Lemkultur in der schlulischen Institution, das Alter, in dem die Sch\"{u}ler$ mit dem Lemen der Zielsprache beginnen. Daraus lassen sich die folgenden unterrichtlichen Konsequenzen ergeben: Die Lehrer sollten sich bemtihen, den $Sch\"{u}lern\;die\;M\"{o}glichkeiten\;f\"{u}r$ die authentischen Kommunikation besorgen, besonders $au{\ss}erhaib$ des Unterrichts, z.B. durch das Chatting- Projekt, $w\"{a}hrend$ im Unterricht mehr $Anl\"{a}sse\;f\"{u}r$ die Reflexion gegeben sollten. Und dabei sollten die unterrichtlichen Vorteile-die gemeinsame Muttersprache und Kultur, das Alter usw. - aktiv benutzt werden. Die meisten Koreaner beginnen $n\"{a}mlich$ mit dem Lernen der zweiten Fremdsprache erst in oder nach der $Pubert\"{a}t$. In diesem Zusammenhang sollte es auch betont werden, dass sich die Autonomie der Lerner durch den Unterricht richtig entwickelt, damit sie $sp\"{a}ter$ ihre Fertigkeiten autonom entwickeln $k\"{o}nnen$.