• 한국동물학회지
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• 제36권2호
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• pp.231-237
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• 1993

Lipophorin (LP) was purified from hemolvmph of late last instar larvae of Hyphuntria cuneo D. by KBr density gradient ultracentrifugation. Chemical composition of LP was investigated by electrophoresis, thin laver chromatography and ryas chromatography. LP consisted of Apo-LP I and Apo-LP ll, and M.W. of them were 230 Kd and 80 Nd, respectivelv. Lipid of LP was mostly composed of neutral lipid including triacylglvcerol, diacvlslvcerol, monoacylglvcerol and free cholesterol, and phospholipid rich in phosphatidvlethanolamine and phosphatidvlcholine. Fatty acids present in these lipids were found to have be 14:0, 16:0, 18:1, and 20:1.

• 한국동물학회지
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• 제35권4호
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• pp.466-473
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• 1992

Storage protein-1 (SP-1) of Gallerio mellonella was identified in hemolvmph and fat body by electrophoresis. SP-1 was purified from hemolvmph by KBr density gradient ultracentrifugation , DEAE-cellulose (DE52) ion-exchange chromatography, and gel permeation chromatography (Sephadex G-200). Purity of SP-1 was confirmed by Non-SDS PAGE and electron microscope. SP-1 is 9.4 nm in diameter and regular octahedron in shape. SP-1 has isoelectric point of 5.7 and native molecular weight of 365 K dalton and is composed of one type of subunit with molecular weight of 82 K dalton. Ttiacylslvcerol and phospholipid were found to be maior lipid components in SP-1.