• Korean Journal of Agricultural Science
• /
• v.48 no.2
• /
• pp.343-351
• /
• 2021

Screening was conducted using 200 kinds of plant extracts to explore herbicide-activated components of plant origin. We separated and purified active substances and elucidated chemical structures using Ligularia stenocephala M., which has strong activity and has not yet been studied. When the solvent fractions of the leaves of Ligularia stenocephala M. were tested for their herbicidal activity, ethyl acetate and chloroform layer showed an inhibition rate of 95.2% and 94.1%, respectively. In particular, the chloroform layer exerted more than 50% herbicidal activity at 10 ppm. From the chloroform layer with the highest herbicidal activity, we isolated three herbicidal active compounds using stepwise chromatography, specifically silica gel or octadecyl silica (ODS) column chromatography, Sep-pak cartridges, and high performance liquid chromatography (HPLC). Based on the analysis of the active compounds using electron ionization mass spectroscopy (EI-MS), ¹H-NMR, and ¹³C-NMR, we identified the active compounds as euparin, 5,6-dimethoxy-2-isopropenylbenzofuran, and liguhodgsonal. When the herbicidal activity of the identified compounds was tested, euparin showed selective herbicidal activity for lettuce at 10^-3 M, and both liguhogsonal and 5,6-dimethoxy-2-isoprophenylbenzofuran exerted selective activity for rice and Echinochloa crus-galli.

• Journal of Korean Society of Water and Wastewater
• /
• v.25 no.5
• /
• pp.741-750
• /
• 2011

A pilot-scale biofilter was constructed to discover degradation characteristics of the complex odor discharged from Ansan wastewater treatment plant. Candida tropicalis for volatile organic compounds, sulfur oxidizing bacteria(SOB) for hydrogen sulfide, and bacteria extracted from feces soil were immobilized on a polymer gel media. According to this study, the EBCT was varied from 36 sec to 18 sec. Toluene was removed as 80% along the variations, but it was recovered as 100% within 1 week. All benzene and xylene were removed during the operation while the efficiency of hydrogen sulfur was temporary decreased at 18 sec of EBCT, thereafter it was recovered to 100% within a week. The maximum elimination capacities of the benzene, toluene, xylene, and hydrogen sulfur were 6.6 g/$m^{3}$/hr, 31.7 g/$m^{3}$/hr, 7.8 g/$m^{3}$/hr, and 133.6 g/$m^{3}$/hr, respectively. There were merits on removal both organic and inorganic complex odor using the pilot-scale biofilter embedded with microorganisms immobilized on polymer gel media.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.520-522
• /
• 2008

A new compound named soraphinol C (1) was isolated from myxobacteria Sorangium cellulosum KM1001 a soil isolate, together with a structurally related known compound, 4-hydroxysattabacin (2). These compounds were isolated by silica gel column chromatography and recycling preparative HPLC, consecutively. The structures of the compounds were determined on the basis of combined spectroscopic analyses. Compounds 1 and 2 exhibited antioxidant activity as a radical scavenger in the experiment using a hydrophilic free-radical initiator, 2,2'-azobis(2-amidinopropane)dihydrochloride with ORAC values of 0.956 and 0.617, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.23 no.3
• /
• pp.152-160
• /
• 1997

It has been used that fluorinated compounds could be applied to Make-up products. It is that fluorinated compounds are hydrophobicity, lipophobicity and homophobicity. These fluorinated compounds are reported to form a highly protective and effective film against most aggressive chemical and physical agents. In this study we maed a success to formulate the new type of lipstic using perfluoropolymethylisopropylether with low molecular weight which is long wearing and comfortable to use. This PPIE was adsorbed more than 90% in this experiment and formed the well-balanced gel networks even if the selected PPIE is 100% volatile at room temperature. On the basis of the adsorption of PPIE, we developed the new type lipstick, which was highly-lip feeling and had long wearing and non coloration. And these characteristics were verified by actual consumer test.

• Food Science and Biotechnology
• /
• v.14 no.2
• /
• pp.223-227
• /
• 2005

Anti-inflammatory assay was conducted on 20 kinds of traditional Indonesian medicinal herbs using soybean lipoxygenase (SLO) and hyaluronidase (HAse). Cinnamomun burmanni Bl showed highest anti-inflammatory activity. Ethyl acetate fraction from methanol extract of C. burmanni Bl bark showing high SLO inhibitory activity was isolated using silica gel-60 column chromatography. Two compounds were isolated and purified through preparative HPLC. Through analyses of UV, $^1H-NMR$, $^{13}C-NMR$, EI-MS and $FAB^+-MS$, compounds 1 and 2 were identified as coumarin and 2-hydroxy cinnamaldehyde, respectively, among which 2-hydroxy cinnamaldehyde showed SLO inhibitory activity of $IC_{50}\;=\;60\;{\mu}M$. Both compounds did not exhibit HAse inhibitory activity.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1998.11a
• /
• pp.130-130
• /
• 1998

Two novel compounds SSC1 and SSC2 were isolated from culture broth produced from the strain of Stereum hirsutum by using of YM broth. They were isolated through HP-20 column chromatography, silica gel column chromatography and preparative HPLC, successively. The molecular formulas of SSC1 and SSC2. were determined as C$\sub$15/H$\sub$22/O$_3$ by high resolution EI -mass. The chemical structures of SSC1 and SSC2 were determined as sesquiterpenoid compounds by spectroscopic analysis of UV, IR, $^1$H NMR, $\^$13/C NMR, DEPT, HMQC and HMBC spectrum.

• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.197.1-197.1
• /
• 2003

To investigate biological active constituents from natural products, we have studied the roots of Angelica koreana Max. (Umbelliferae). Fifteen compounds were isolated from the MeOH extract by column chromatography on a silica gel. The compounds were identified as isoimperatorin, oxypeucedanin, oxypeucedanin hydrate, osthol, nodakenin, 2-hydroxy-4-methylacetophenone, cimifugin, falcarindiol, heraclenin, pabulenol, umbelliferone, demethylsuberosin, hamaudol, sec-O-glucosylhamaudol, and prim-O-glucosylcimifugin, respectivelv, by spectroscopic means. Among these, the latter eight compounds were isolated for the first time form this plant.

• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.190.2-190.2
• /
• 2003

In the course of search for various bioactive compounds from marine algae, we found strong antioxidant activity of the methanolic extract of the brown alga G. elliptica. Chromatographic purification [ODS flash, gel-filtration on Sephadex LH-20, HPLC] of the BuOH layer of the methanolic extract afforded two known polyphenolic compounds, 6,6'-bieckol (1) and dieckol (2). Compound 1 showed acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities, free radical scavenging activity on DPPH (1,1-diphenyl-2-picryl-hydazyl) with IC$\_$50/ values of 91.2, 45.6 and 15.5 $\mu\textrm{g}$/$m\ell$, respectively. (omitted)

• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.202.1-202.1
• /
• 2003

Six compounds were isolated from the 90% MeOH fraction of the vine stem of Spatholobus suberectus Dunn (Leguminosae) using silica gel, reverse phase column chromatography and RP-HPLC. Structures of compounds 1-6 were elucidated by spectroscopic parameters of IR, E1 MS, FAB MS, lD-NMR and 2D-NMR spectrum and identified as pseudobaptigenin (1), genistein (2), (2R)-7-hydroxy-6-methoxyflavanone (3), (3R,4R)-2",4"-dihydroxy-6,7-methylenedioxy-isoflavan-4-ol (4), (3R,4R)-7,2" -dihydroxy- 4"-methoxyisoflavan-4-ol (5), sativan (6), respectively. Compounds 3, 4 and 5 have been newly reported in nature.

• Journal of the Korean Wood Science and Technology
• /
• v.30 no.3
• /
• pp.42-47
• /
• 2002

This study was carried out to investigate the constituents of Sophora japonica(Leguminosae) bark. To isolate compounds, bark was extracted with ethanol and then partitioned with hexane, dichloromethane, ethylacetate and butanol successively. After partitioned, DCM fraction was subjected to column chromatography with various solvent system in silica gel and/or Sephadex LH-20. Structures were elucidated by spectroscopic methods including MS, ¹H, ¹³C and 2D-NMR experiments. Four compounds were isolated from the bark and identified as 3',7-dihydroxy-4'-methoxyisoflavone (cyclosin), puerol A, maackiain-3-O-𝛽-D-glucopyranoside (trifolirhizin), and 4', 5, 7-trihydroxyisoflavone (genistein). Among these compounds, cyclosin and trifolirhzin were first isolated from S. japonica.