• Preventive Nutrition and Food Science
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• v.15 no.2
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• pp.143-146
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• 2010

Organic zinc was included in the diet of broiler chickens to examine its effect on the skin characteristics and the expression level of skin proteins. Broiler chicks (Ross$\times$Ross) were fed a corn-wheat-soybean meal basal diet, either as control or containing an additional 80 ppm of zinc proteinate for 4 weeks, and then five broilers from each treatment were selected randomly, slaughtered, and their skin characteristics were examined. There were significant increases (p<0.05) in thigh skin epidermis and dermis thickness in the chicks fed organic zinc. Collagen content in the skin of broilers was also increased by the addition of organic zinc to the diet. 2D-gel electrophoresis patterns indicated that expression levels of the three proteins, glyoxylase 1, hypothetical protein, and dispersin B were affected by zinc feeding. These results suggest that adding organic zinc to the chicken's feed may contribute to decreased skin tearing.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.980-985
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• 2000

Influences of Japanese green tea powder (GTP) supplementation to commercial diet on laying performance and egg quality were studied by using 60 laying hens. The experimental diet with or without 0.6% GTP was given ad libitum to the birds during the period from 6 to 71 weeks of age. The birds started egg production from 21 wk of age regardless GTP feeding. Body weight, feed intake, egg weight tended to decrease with GTP supplementation, while egg production rate tended to increase. Haugh unit score was significantly increased with GTP, which accompanied with the increased albumen height. These were observed almost throughout the laying period over 50 wk. Gel proportion in thick albumen was decreased as storage time is prolonged, then higher values of the gel proportion were recorded in the eggs from GTP group. Thick albumen of the eggs from GTP-fed layers had more carbohydrate than that from control layers. All these indicate physical stability of thick albumen in the eggs from GTP group. Thiobarbituric acid content of egg yolk tended to remain lower in the eggs from GTP group during 5-10 days of storage at room temperature. Levels of egg yolk cholesterol and yolk lipid were significantly reduced by GTP feeding. There were no significant differences in eggshell weight, shell thickness and shell strength between the two groups. Thyroid gland and liver from hens slaughtered at 71 wk of age did not differ in weight irrespective of GTP feeding. The present results suggest that GTP could modify components of edible part of egg, leading to the characteristics favourable to consumers such as high durability of thick albumen and less cholesterol in yolk, without altering general performance of the layers throughout this year round experiment.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.3
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• pp.325-330
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• 1995

Seaweed Jellys(Muks) were prepared with sea mustard and sea tangle, Optimum conditions for preparation of seaweed Muks with soy protein were investigated. Gel strength of Muks with sea mustard and soymilk decreased as the quantity of soy milk increased, and increased as the moisture content of soy milk had been decreased. Optimum mixing ratio of seaweed and soy milk was 7 : 1 and optimum temperature of gelation was $65^{\circ}C$. Gel strength of seaweed Muk mixed $5\%$(w/w) of soy protein isolate was higher$(900g/cm^2)$ than those of seaweed Muks with and without soy milk.

• Korean Journal of Pharmacognosy
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• v.40 no.2
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• pp.109-117
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• 2009

We examined the purity of six heparin samples by using heparinase, chondroitinase, $^{1}H-NMR$, and polyacrylamide gel electrophoresis. To obtain high molecular weight contaminants from heparin samples, heparinase I - digested samples were subjected to the exhaustive microcon filtration. The filtration process removed heparin-derived di- and oligosaccharides effectively. By combining chondroitinase ABC treatment and strong anion exchange - high performance liquid chromatography, the result showed all six samples contained chondroitin sulfate as a contaminant ranging from 1.3 to 14.9%. Among them, sample S3 showed the highest content of 14.9%, which was further analyzed by chondroitinase AC treatment to confirm chondroitin sulfate B (dermatan sulfate). $^{1}H-NMR$ chemical shifts of N-acetyl groups clearly suggested the existence of chondroitin sulfate B (sample S3) and oversulfated chondroitin sulfate (samples S2 and S4) as contaminants. In addition, polyacrylamide gel electrophoresis was useful for qualitative detection on the sample's purity. These results suggest that the tools of heparinase I and chondroitinase ABC in combination with NMR spectroscopy would give very useful information for investigation of heparin contaminants such as oversulfated chondroitin sulfate and dermatan sulfate in heparin samples.

• Food Science of Animal Resources
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• v.23 no.2
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• pp.155-160
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• 2003

The purpose of this study was to purify epidermal growth factor(EGF) as growth factor from human colostrum. The effects of purified EGF fraction were directly related to the growth of cells. Results were as follows; After eliminated fat from colostrum, skim milk was obtained. We obtained the EGF fraction by performing ultrafiltration and gel filtration, and then were convicted by SDS-PAGE. The result of analysis of purified EGF fraction by high performance liquid chromatography(HPLC) was shown a peak at 31.185 min period. And it was similar with standard EGF that was shown a peak at 31.545 min. 10 ng of EGF was contained in 10 mg/mL through immunoassay to measure EGF content from isolated fraction. After SDS-PAGE, isolation degree of purified fraction was convicted through western blotting. BALB/3T3 cell was the most effectively stimulated and proliferated at 1 mg/mL concentration of the purified EGF fraction and percentage of cell proliferation was about 95%. In the case of IEC-6 cell, that was about 71%.

• Polymer(Korea)
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• v.26 no.1
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• pp.61-70
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• 2002

The effects of molecular weights and rheological properties of polypropylene (PP), on its foam structures in batch process were investigated. The effects of crosslinking process were also considered in this study. The rheological properties of polypropylene, such as storage modulus(G'), loss modulus(G"), zero shear viscosity($\eta_O$), and relaxation time($\lambda$), increased with the increase of molecular weights, and these increases in rheological properties directly affected the stability improvements of the PP foam. The increase of crosslinked PP's gel content stopped at the irradiation dose of 3.2 Mrad. The development of foam structures was more enhanced as the irradiation dose increased up to 3.2 Mrad. When the irradiation dose exceeded 3.2 Mrad, however, it negatively affected the structural development of the foam by diminishing gel contents of the foaming material, which resulted in instability of the foam structure.ture.

• Bulletin of the Korean Chemical Society
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• v.24 no.3
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• pp.299-305
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• 2003

Physical properties and microstructure of new coumarin 4 doped $SiO_2$-PDMS ORMOSILs, synthesized by one-step (OS, acid-catalysis) and two-step (TS, acid-base catalysis) routes of sol-gel method with varying pH (0.6 to 7) and dye content $(5\;{times}\;10^{-4}\;to\;5{\times}\;10^{-2}\;mole)$, are reported. BET, UV-visible spectroscopy and SEM were used for characterizations. The increase in acid or base concentration increased the size of pores and aggregated silica particles. The samples with pH ≤ 2.5 were transparent and attributed to the small size of pores (~20 Å) and silica particles. The samples with pH > 2.5 were translucent or opaque due to non-uniform pore system formed by voids and large aggregated silica particles. The surface area was found a key factor controlling the interactions between the gel matrix and the dye. The OS samples with the highest dye concentration exhibited the minimal values of pore size, surface area and silica particle size, resulting in the concentration-quenching phenomenon.

• Polymer(Korea)
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• v.27 no.6
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• pp.555-561
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• 2003

The self-assembled monolayer coating of octadecyltrichlorosilane (OTS) on the silicon based MEMS was investigated and surface characteristics were considered as a function of coating conditions and reagent composition. The sulfuric peroxide mixture (SPM) solution was used to form -OH group which caused the hydrophilic characteristic on silicon surftce. Highest hydrophilicity was obtained by SPM solution with 85% acid content at room temperature. OTS was applied on the silicon surface by means of self-assembled monolayers (SAMs) coating. It was found that sol-gel reaction was took place between -OH group on the silicon surface and -Cl group in OTS. As a result, the contact angle increased due to the increase of hydrophobicity by Si-O bonding of SAMs. Sol-gel reaction could be controlled by coating conditions as well as reagent composition in OTS coating solution.

• Solar Energy
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• v.15 no.3
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• pp.15-27
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• 1995

This study presents experimental and numerical results of the temperature characteristics, the heat transfer phenomema, and the heat storage quantity during the heat storage process with sodium phyrophosphate decahydrate($Na_4P_2O_7{\cdot}10H_2O$)-Phase Change Material(PCM) in a latent heat storage tank(cubic type). It was proved that heat transfer by conduction was dominant because PCM($Na_4P_2O_7{\cdot}10H_2O$) during heating processes was gel phase, not liquid phase The gap ratio(rate of air content) of PCM became smaller, the thermal capacity of PCM became larger, therefore the temperature distribution of PCM slowly increased than that of large gap ratio. There was maximum 15% difference between measured temperatures and calculated temperatures.

• Journal of Life Science
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• v.19 no.8
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• pp.1125-1131
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• 2009

The antimicrobial activities of Helicobacter pylori as a functional food source with water and 60% ethanol extracts from Rododendron mucronulatum Turcz. flowers were examined. The total phenol content of 60% ethanol extracts (30.6${\pm}$0.14 mg/g) from Rododendron mucronulatum Turcz. flowers was higher than that of water extracts (23.2${\pm}$0.21 mg/g). The inhibitory activities of Rododendron mucronulatum Turcz. extracts on H. pylori was determined to clear zone of 15 mm in 80% ethanol extracts. Purification of inhibitory compounds was carried out in Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient procedure, with increasing ethanol(0${\rightarrow}$100%) in $H_2O$. The chemical structure of the purified inhibitory compounds of H. pylori was identified to be quercitrin (quercetin-3-O- rhamnopyranoside), myricitrin (myricetin-3-O-rhamnopyranoside), quercetin by FAB-MS, NMR and IR spectra.