• Title/Summary/Keyword: Gel Container

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Isoenzyme patterns and phylogenetic relationships in Acanthamoeba spp. isolated from contact lens containers in Korea

  • Shin, Ho-Joon;Cho, Myung-Soo;Kim, Han-jip;IM, Kyung-Il
    • Parasites, Hosts and Diseases
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    • v.37 no.4
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    • pp.229-236
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    • 1999
  • In order to refer to the basic information regarding the identification of isolates obtained from a contact lens container in Korea, the isoelectric focusing gel electrophoresis was employed to compare the isoenzyme band patterns among Acanthamoeba spp. including eight isolates and the simple pairwise dissimilarity analysis was carried out. For an alkaline phosphate development, isolate 7 and Acanthamoeba polyphaga showed homologous band patterns, and isolates 1, 2, and 3 showed the same patterns. For lactate dehydrogenase, similar patterns were observed in isolates 2 and 3. Isolates 3 and 5 showed homologous band patterns for malate dehydrogenase and glucose phosphate isomerase. For hexokinase, isolates 4, 7, and A. hatchetti showed the same band patterns. In others, a considerable number of interstrain polymorphisms was observed in nine isoenzyme band patterns. In Acanthamoeba group II, genetic distances among isolates 1, 2, 3, 4, and 5 ranged from 0.104 to 0.200. In comparison to A. castellanii, A. hatchetti, and A. poIyphaga, genetic distances of isolates 7 and 8 were 0.254 and 0.219, respectively. In Acanthamoeba group III, including A. culbertsoni, A. healyi, and A. royreba, isolate 6 had genetic distances which ranged from 0.314 to 0.336. Finally, when comparing to the six reference Acanthamoeba, it was possible to classify isolates 1, 2, 3, 4, and 5, as genetically close-related species and as independent species group. Furthermore, isolates 6, 7 and 8 were identified as independent species as well.

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3-Dimensional Verification Technique for Target Point Error (자기공명영상기반 겔 선량측정법을 이용한 3차원적 목표 중심점 점검기술)

  • Lee, Kyung-Nam;Lee, Dong-Joon;Suh, Tae-Suk
    • Progress in Medical Physics
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    • v.22 no.1
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    • pp.35-41
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    • 2011
  • For overall system test, hidden-target test have been used using film which leads to inherent analysis error. The purpose of our study is to quantify this error and to propose gel dosimeter based verification technique for 3-dimensional target point error. The phantom was made for simulation of human head and this has ability to equip 10 gel-dosimeter. $BANGkit^{TM}$ which we are able to manufacture whenever it is needed as well as to easily change the container with different shapes was used as a gel dosimeter. The 10 targets were divided into two groups based on shapes of areas with a planned 50% isodose line. All treatment and analysis was performed three times using Novalis and $BrainSCAN^{TM}$. The target point error is $0.77{\pm}0.15mm$ for 10 targets and directional target point error in each direction is $0.54{\pm}0.23mm$, $0.37{\pm}0.08mm$, $0.33{\pm}0.10mm$ in AP (anterior-posterior), LAT (lateral), and VERT (vertical) direction, respectively. The result of less than 1 mm shows that the treatment was performed through each precise step in treatment procedure. In conclusion, the 3-dimensional target point verification technique can be one of the techniques for overall system test.

A Study on the Necessity Verification of Convex Probe Disinfection (Convex Probe 소독 필요성 검증에 관한 연구)

  • Choi, Kwan-Yong;Yoo, Se-jong;Lee, Jun-ho;Hong, Sung-Yong
    • Journal of the Korean Society of Radiology
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    • v.13 no.2
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    • pp.193-200
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    • 2019
  • The study was conducted surveying ultrasound room workers on hospital infection awareness in Daejeon and Choong-chunng region. The contamination of ultrasonic probes used in clinical trials was measured using ATP, and the results were verified after using 70% alcohol sterilization. It was measured on the group's general characteristics and the specific categories such as academic background, job type, having professional certificate and infection education. After the examination, the gel removal and method, disinfection status of the probe and variable correlation analysis were performed to analyze the recognition of the ultrasonic probe disinfection. After examination in ultrasound room, it was found that towels were used the most for cleaning, and the gel container was not replaced for more than three months. After 70% alcohol disinfection, ATP contamination was reduced from $1055.4{\pm}944.2$ to $133.5{\pm}93.2$ and the result was analyzed to be statistically significant.(${\rho}<0.01$) The found bacteria were CNS, Gram positive bacillus, and Micrococcus specs. In order to solve this problem, 70% alcohol sterilization was applied and the bacteria were not detected after the treatment. The research shows that regular training on infection control and efforts to prevent infection are necessary, and that 70% alcohol is effective in disinfect the bacteria. Therefore, the medical institution should provide active hospital infection control education to improve the awareness of hospital infection among workers and contribute to the prevention of patient infection. It is also understood that proper use of the results of this study will help prevent infection by means of ultrasonic probes.

A Close Examination of Unstability and a Quality Improvement using Anhydrous $Na_2CO_3$ in Waste Plastic's Thermal Pyrolysis Oil (폐플라스틱 열분해 재생유의 불안정한 요인 규명과 무수탄산나트륨으로 품질 향상)

  • Seo, Young-Hwa;Ko, Kwang-Youn
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.12
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    • pp.1371-1380
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    • 2007
  • Study on the instability of waste plastic's thermal pyrolysis oil was carried out for the purpose of improving its quality. The reaction of pyrolysis oil with ozone changed double bonds into aldehydes and ketone, estimated that HDPE pyrolysis oil contained $\sim45$ wt% 1-alkene type olefins, and PP pyrolysis oil did $\sim73$ wt% olefins, which consisted of $\sim47$ wt% secondary and $\sim20$ wt% primary alkenes. The dark brown color and odor of pyrolysis oil were improved by eliminating double bonds, indicated that they were directly related to unsaturated hydrocarbons. Container test showed that metal can affected oil quality worse than the brown glass bottle. Antioxidant added into pyrolysis oil was consumed up to 90% within $2\sim3$ days and the wt. composition of unsaturated hydrocarbons in pyrolysis oil was not changed within 50 days, inferring that instability of pyrolysis oil due to unsaturated bonds can be stabilized by antioxidants. Adsorption test on silica gel, activated carbon and alumina to remove precipitates in oil produced a good result, but not enough to remove moisture. However, cheap anhydrous sodium carbonate showed the best removal efficiency of moisture as well as precipitates in oil. Therefore the pyrolysis oil quality improvement was accomplished by applying anhydrous $Na_2CO_3$ into the production plant.