• 한국임상수의학회지
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• 제26권2호
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• pp.150-154
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• 2009

최근 doctor fish(Gara rufa obtusa)를 이용한 Ichthyotherapy는 인간 피부질환의 대체 치료법으로 각광받고 있으며, 근래 doctor fish의 국내 증식 성공으로 인하여 이러한 추세는 증가되고 있다. 그러나 doctor fish의 질병과 생태에 대해서는 아직 연구가 미미한 실정이다. 본 보고는 국내에서 증식되던 doctor fish가 Citrobacter freundii에 감염되어 집단 폐사한 예로서, 병어에 대한 외부 및 내부 진단, 분리된 세균의 형태학적, 생화학적 동정을 통하여 C. freundii를 확정하였으며, 분리된 C. freundii에 대한 항생제 감수성 검사를 수행하였다. 또한 살아남은 병어에 대한 집단 치료를 시도하여 성공적인 치료 효과를 얻을 수 있었다.

• 한국해양생명과학회지
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• 제1권1호
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• pp.18-24
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• 2016

Since April 2012, doctor fish in the breeding tank and in the quarantine tank in Hanwha Aquaplanet Yeosu Aquarium have been dying, accompanied by diffuse bleeding around the mouth, in the chin, and at the bottom of the abdomen. In this study, the cause of death would be examined through the bacteriological study of doctor fish and the rearing water quality in the aquarium. The water quality and the bacterial counts of the rearing water in the exhibit tank and in the quarantine tank were analyzed once a week, starting from August to November 2014. Water quality was measured based on the following data: temperature was in the range of 24.5~26.8℃, pH at 6.77~7.94, DO at 6.15~8.61 ppm, ammonia at 0~0.93 ppm, nitrite at 0.009~0.075 ppm, and nitrate at 1.1~40.9 ppm. Studies revealed that the differences in these water quality factors were not related to the death of doctor fish. Bacterial counts in the rearing waters of Garra rufa slightly increased to 10³~10⁴ CFU/ml, just before the death of the doctor fish. Twelve strains of bacteria were isolated from the dead fish and rearing waters. The isolates were identified as Aeromonas veronii, Citrobacter freundii, Pseudorhodoferax aquiterrae, Shewanella putrefaciens, and Vibrio anguillarum on the basis of 16S rRNA gene sequences. The most dominant species was C. freundii, which showed medium sensitivity to florfenicol and norfloxacin, and was resistant to amoxacillin, doxycycline, oxytetracycline, tetracycline, and trimethoprim. Ten isolates were confirmed to be pathogenic to the doctor fish. Doctor fish infected with C. freundii and S. putrefaciens showed high mortality in the experimental groups. These results indicate that the variation in bacterial numbers in the rearing water was related to the death of doctor fish. C. freundii and S. putrefaciens were directly implicated in causing the death of doctor fish in the aquarium.