• 한국버섯학회지
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• 제22권1호
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• pp.31-36
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• 2024

친환경적인 플라스틱 분해를 위한 연구의 한 종류로 버섯균을 활용한 플라스틱 분해 유도를 위해 본 실험을 진행하였다. 본 실험에서는 주변에서 구하기 쉽고 인체에 유해할 가능성이 낮은 식용 버섯을 활용하여 인체 안정성을 더한 플라스틱 분해 기술을 개발하고자 하였으며 31 종의 버섯균들을 대상으로 플라스틱 PE, PS, PET 필름에 대한 분해 효과를 관찰하였다. 본 연구과정에서 4종의 버섯(종이비늘버섯, 영지버섯, 갈색먹물버섯, 느타리)에 의한 플라스틱 분해 효과를 관찰하였으며, 진균과의 교차 배양을 통해 플라스틱 분해 효과를 촉진시킬 수 있는지를 확인하였다. 해당 확인 과정에서 PS에 대한 분해 효과가 가장 높게 나타났으며 이는 진균 Asp. nidulans의 작용에 의한 것이었다. 그러나 Asp.nidulans가 가진 유해성 (Henriet et al., 2012)으로 인해 인체에 무해한 식용버섯균의 활용이 필요하다고 판단되며 비록 버섯균만을 활용한 분해 결과는 저조하나 진균과 버섯균을 교차 배양한 분해 효과는 Asp. nidulans에 의한 분해 효과에 근접하다고 볼 수 있다. 또한 PET에 대해서는 오히려 PT_2822_nig의 사례와 같이 교차 배양한 샘플이 더 높은 수치의 플라스틱 분해 효과를 보였다. 비록 두 실험 결과값들이 유의성을 보이지 못해 추가적인 보완실험이 요구되고 있으나 해당 실험을 통해 버섯균을 활용한 플라스틱 분해 유도 또한 세균, 진균, 밀웜 등을 활용한 분해 유도 과정과 비교하여 경쟁성을 보이고 있다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.13-13
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• 2014

Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.

• 한국균학회지
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• 제32권2호
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• pp.142-144
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• 2004

Helicobacter pylori에 대한 항균효과는 열수추출물에서는 Fomitopsis pinicola가 15 mm의 생육저해환으로 가장 높았으며 그 다음으로는 Codyceps militaris, Coriolus versicolor, Phellinus linteus, Fomitella traxinea가 비교적 높은 항균력을 나타내었다. 열수 추출물들의 pH가 낮았으나 항균력이 없는 것으로 보아서 버섯추출물의 수소이온 농도는 항균력에 영향을 미치지 않는 것으로 여겨졌다. Methanol 추출물에서도 Fomitopsis pinicola가 Helicobacter pylori에 대한 생육저해환의 크기가 44 mm로 가장 높은 항균력을 보였다. 이미 알려진 바와 같이 버섯의 주성분은 당과 단백질이며 주로 단백다당체를 이루고 있다. 이를 구성하는 성분 및 함량에 따라 다른 기작을 보이는 갓으로 추정되며 결합하고 있는 형태도 기작에 영향을 끼치는 것으로 사료된다. 본 실험을 통해 Fomitopsis pinicola methanol 추출물이 DEAE-Sepadex의 초기 분획에서 Helicobacter pylori에 대한 항균효과를 나타냈다. 이 분획은 당과 단백질의 함량이 높게 나타났다. Fomitopsis pinicola, Codyceps militaris는 Helicobacter pylori에 대한 항균력이 뛰어나 기능성식품에 이용될 수 있을 것으로 기대된다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.222-222
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• 1996

In order to elucidate the correlation between the lipid peroxidation and hepatotoxicity, the formation of malondialdehyde (MDA) in liver homogenate and serum, and the transaminase activities were determined in intoxicated by ascorbic acid-Fe$\^$2+/-ADP in rat. In a model of ascorbic acid-Fe$\^$2+/-ADP hepatotoxicity, G009, which was obtained from Ganoderma lucidum IY009, exhibited anti-lipid peroxidative effect in rat liver homogenate, and that MDA values of the liver homogenate decreased from 48.1% to 74.8% in comparision to controls (p<0.01) Also, the MDA formation in serum inhibited 66.5% at 100 mg/kg of G009. Serum levels of glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) in peroxidizer-induced rats treated with G009 was decreased compared with control. Especially, The formation of lipid peroxides in serum was related to GPT levels. These results that G009 has a protective effect on ascorbic acid-Fe$\^$2+/-ADP-induced hepatic injury through an inhibition of lipid peroxidation in liver.

• 대한한의학회지
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• 제28권3호통권71호
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• pp.86-99
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• 2007

Objectives : Cancer patients who are afraid of unpleasant therapy and doubt a perfect cure often seek out traditional Korean medicine or many other kinds of complementary and alterative medicine (CAM) instead. This study was carried out in order to learn the prevalence and kinds of the traditional Korean medicine and CAM to evaluate awareness of and attitude toward it. Methods : 213 patients with gastric cancer who had undergone surgery in the department of gastrointestinal surgery and visited for routine follow-up from November to December of 2004 were included. A questionnaire survey was done by trained interviewers for CAM and demographic information. Results : 96.7% of patients had experience with CAM. The kinds and frequencies of CAM were diet and nutrition 21.0%, folk remedy 21.0%, pharmacological treatment 13.5%, oriental medicine 5.3% and mind-body intervention 1.4%. 56.8% of patients had experienced 2-6 kinds of CAM, 37.4% more than 7. Among diet and nutrition, ginseng (62.9%) was highest, followed by ox leg bone soup (50.8%) and pumpkin (31.5%). Among the folk remedies, elm tree was highest at 46.5%, followed by phellinus linteus (Sang-Whang) at 26.8% and ganoderma lucidum (Young-Gee) 20.7%.satisfaction with CAM was relatively low (43.2%), many patients (78.2%) replied that they would use it continuously. Conclusion : Most patients had experience with many kinds of CAM, but didn't discuss it with medical doctors. If scientific study proves whether or not each kind of CAM is actually effective ondisease, it will help to improve the health of patients and prevent the inappropriate usage and cost.

• 한국버섯학회지
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• 제13권3호
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• pp.170-174
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• 2015

식용버섯인 팽이버섯, 꽃송이버섯, 참송이버섯, 느타리 버섯, 녹각영지, 상황버섯, 눈꽃 동충하초, 아가리쿠스, 잎새버섯, 석이버섯, 노랑느타리버섯, 분홍느타리버섯, 편각영지버섯, 현미 동충하초, 번데기 동충하초, 표고버섯의 물 및 에탄올 추출물에 대한 피부염증완화 효능을 확인한 결과 열수추출물에서는 잎새버섯과 번데기 동충하초가 피부염증완화 효능이 탁월하였으며, 에탄올 추출물에서는 영지버섯, 표고버섯, 번데기 동충하초와 팽이버섯에서 피부염증완화 효능이 탁월하였다. 특히, 각각의 추출물에서 피부염증완화 효능이 높은 것은 번데기 동충하초 임을 확인하였다.

• 생약학회지
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• 제39권3호
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• pp.213-217
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• 2008

Oxidative stress is considered to play an important role in a variety of neurodegenerative disorders of central nervous system. The immortalized mouse hippocampal cell line, HT22, phenotypically resembles neuronal precursor cells but lacks functional ionotropic glutamate receptors, thus excluding excitotoxicity as a cause for glutamate triggered cell death. Therefore, HT22 cells are a useful model for studying oxidative glutamate toxicity. In this study, we examined whether the methanol extracts of some native plants at Mt. Baekdu could protect HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Seventy-eight plants sources were collected at Mt. Baekdu, and extracted with methanol. These extracts had been screened the protective effects against glutamate-induced oxidative damage in HT22 cells at the 100 and 300 ${\mu}g/ml$. Of these, thirteen methanolic extracts, Acer mono (leaf), Artemisia stolonifera (aerial part), Carduus crispus (aerial part), Carex mongolica (whole plant), Clematis hexapetala (whole plant), Galeopsis bifida (aerial part), Galium verum (whole plant), Ganoderma lucidum (whole plant), Ixeris chinensis (whole plant), Malva verticillata (aerial part), Polygonum senticosum (whole plant), Rebes mandshricum (branch), and Taraxacum mongolicum (aerial part), showed significant protective effects against glutamate-induced oxidative damage in HT22 cells.

• 한국식품조리과학회지
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• 제22권3호통권93호
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• pp.337-345
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• 2006

영지버섯, 장수버섯, 상황버섯을 곡물에서 배양하는 고체재료 발효방법을 개발하였으며, 발효된 곡물을 이용하여 즙장을 제조하고 관능검사를 실시함으로써 기능성식품으로서의 이용 가능성을 검토하였다. 담자균의 고체재료 배양을 위하여 사용된 곡물의 특성을 조사한 결과, 냉침시간에 따른 곡물의 수분함량 변화는 메주콩이 10시간, 보리는 6시간, 율무가 4시간, 밀이 12시간만에 최대수화 시간에 도달하였으나 최종 수분함량이 $30{\sim}48\;%$로서 버섯 균사체가 생장하기에는 부족한 수분함량을 보였다. 담자균사체의 대량배양 조건실험을 실시한 결과, 냉침에 의해 최대수화에 도달한 곡물을 배양용기에 담고, 액체배양 후 균질화한 담자 균사체를 곡물에 접종함으로써 접종초기 짧은 시간에 균사가 완전히 활착되도록 할 수 있었다. 또한 균사 배양중기에 멸균 증류수를 첨가함으로써 균사의 활력을 유지시킬 수 있었다. 곡물에 배양된 담자균의 균사체량을 나타내는 glucosamine의 함량은 장수버섯과 상황버섯이 배양된 율무에서 특히 높은 함량을 나타내었고 영지버섯이 배양된 곡물의 경우는 밀에서 가장 높은 함량을 나타내었다. 담자균사체에 의해 발효된 곡물을 이용하여 즙장을 제조하고 관능검사를 실시한 결과, 영지버섯 균사체가 배양된 곡물이 가장 우수하였는데, 그 중 콩에 율무를 혼합한 시료가 가장 우수한 것으로 나타났으며, 숙성기간이 길어짐에 따라 전반적으로 기호도가 약간씩 상승하였다.

• 한방안이비인후피부과학회지
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• 제29권4호
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• pp.1-13
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• 2016

Objectives : The purpose of this study is to compare hair and alopecia of Korean Medicine with those of western medicine. Methods : We studied relationships between hair and essense(精), qi(氣), blood(血), five vicera(五臟) and meridians(經絡) through literature review about hair and alopecia. We compared Korean medicine with western medicine on physiology of hair and treatment of alopecia based on the study. Results & Conclusion : 1. Congenital essense(先天之精) is related with genetic factor and acquired essence(後天之精) is connected with nutritional factor. Defending function of Defense qi(衛氣) is related with immune reaction and qi stagnation(氣鬱) is associated with stress reaction. Atrophy of vascular tissues observed in alopecia scalp means deep relationship between blood(血) and alopecia, further deficiency of blood can cause telogen effluvium. 2. Kidney qi(腎氣) is related with inhibiting combination of Androgen receptor and Dihydrotestosterone(DHT) or activating hair growth factior. Pi(脾) is connected with alopecia seborrheica caused by damp-heat(濕熱) and alopecia areata caused by excessive prudence(思慮過度). Heart(心) is associated with atrophy in vascular tissue of scalp and liver(肝) is connected with metabolism. 3. Armpit hair and pubic hair as secondary sex characteristics are realated with Yangming Meridian(陽明經) and beard and hair at crown part where Type II $5{\alpha}$-reductase is activated much are associated with Taiyang Meridian(太陽經). 4. Juglandis Semen pharmacopuncture and Ganoderma lucidum pharmacopucture have better effects on inhibit $5{\alpha}$-reductase than Finasteride. Minoxidil and PRP are similar with promoting blood flow and removing stasis(活血祛瘀). Seven-star needling(七星針) is similar with microneedling. 5. Alopecia can be caused by due to lack of circulation Views we need solution to improve circulation coincide in opinion with Korean Medicine and Western medicine.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.244-250
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• 1996

In this study, the anti-lipidperoxidative effects of G009, a polysaccharide extracted from Ganoderma lucidum IY009, was determined in ascorbic acid-$Fe^{2+}$-adenosine 5-diphosphate-intoxicated rat. In a model of ascorbic acid-Fe$^{2+}$-adenosine 5-diphosphate-induced hepatotoxicity in rat, G009 exhibited anti-lipidperoxidative effect in rat liver homogenate, and that malondialdehyde values of the liver homogenate inhibited from 48.1% to 74.8% in comparison to controls (p<0.05). The malondialdehyde formation in serum inhibited 66.5% at 100 mg/kg of G009. Also, serum levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in peroxidizer-induced rats treated with G009 was decreased compared with control. Especially, the formation of lipid peroxides in serum was related to glutamic pyruvic transaminase levels. These results suggest that G009 has a protective effect on ascorbic acid-$Fe^{2+}$-adenosine 5-diphosphate-induced hepatic injury through an inhibition of lipid peroxidation in liver.r.