• 제목/요약/키워드: Ganoderma lucidum

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영지(靈芝)의 비자실체성(非子實體性) 담자포자(擔子胞子)의 형성(形成)에 미치는 광(光)의 영향 (Effect of Light on the Formation of Non-Basidiocarpous Basidiospores of Ganoderma Lucidum)

  • 신관철;서건식
    • 한국균학회지
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    • 제17권4호
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    • pp.189-193
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    • 1989
  • 영지(靈芝)버섯 균사의 비자실체성(非子實體注) 담자포자(擔子胞子)의 형성(形成)에 미치는 광(光)의 영향을 구명(究明)하기 위하여 광질(光質), 광도(光度) 및 광조사(光照射) 시간(時間)을 달리하여 실험하였다. 영지(靈芝)버섯 균사의 비자실체성(非子實體注) 담자포자(擔子胞子)의 형성(形成)에 있어서 광(光)은 필수 요인이었다. 광(光)의 조사(照射)는 16시간 광(光)/8시간 암(暗)으로 계속 처리할 때 포자(胞子)의 형성량(形成量)이 가장 많았고 유효(有效)한 광(光)은 blue와 yellow이었으며 Near U.V.에서는 포자(胞子)가 형성(形成)되지 않았다. 광도(光度)는 백색광으로 1,000Lux일 때 가장 효과적(效果的)이었다. 광형태(光形態) 형성(形成) 반응(反應)은 계통간(系統間)에 명확한 차이를 보여 광(光) 요구성(要求性)이 유전인자(遺傳因子)의 지배를 받음을 보였다.

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큰느타리(King Oyster Mushroom) 재배시스템을 활용한 녹각영지버섯(Ganoderma lucidum) 적정 재배온도 (Appropriate temperature of antler-shaped Ganoderma lucidum using King Oyster Mushroom Cultivation System)

  • 조우식;김민경;황재순
    • 한국버섯학회지
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    • 제20권3호
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    • pp.183-185
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    • 2022
  • 여름철 버섯소비 부진으로 일부 농가에서는 여름철에 생산을 중단하는 등, 고소득 버섯작목 복합영농의 필요성이 제기됨에 따라, 녹각영지버섯을 새송이버섯 재배사를 이용하여 생산 가능 여부를 확인하고자 시험을 실시하였다. 배지재료로 사용한 참나무톱밥과 미강의 화학적 특성을 조사한 결과 T-N은 주재료인 참나무톱밥이 0.28%, 영양원인 미강이 2.2%로 나타났으며, pH는 참나무톱밥 6.0, 미강 6.6로 주재료인 참나무톱밥은 약산성을 나타내었고, 영양원인 미강은 중성을 나타내었다. 녹각영지버섯 자실체 수량특성을 조사한 결과, 초발이 소요일수는 25℃ 5~6일, 30℃ 3~5일이었으며, 대의 길이는 25℃에서 57.5 mm, 30℃에서 92.2 mm였고, 생체중은 25℃에서 43g, 30℃에서 50 g으로 30℃에서 우수하였다. 본 시험을 통하여 하절기에 새송이버섯 재배사를 녹각영지버섯 재배에 활용이 가능함을 확인하였다.

식용 버섯류의 무기물 함량 (Studies on the Mineral Content of Edible Mushrooms)

  • 허윤행;김옥경
    • 한국환경보건학회지
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    • 제17권1호
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    • pp.129-135
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    • 1991
  • To investigate on the trace element content of twelve edible mushrooms and Aloe arborescens, i. e., Lentinus edodes, Ganoderma lucidum (culturing in wood and soil), Tricholoma matsutake, Agaricus bisporus, Cyrophora esculenta, Auricularia auricula-Jude (produced in Korea and China), Sarcodon asparatus, Pleurotus ostreatus, Coriolus versicolor, Smilax rotundifolia and Aloe arborescerts were analyzed by Atomic absorption spectrometer. The obtained results were summerized as follows: 1. Potassium, sodium, magnesium and iron content for the most part samples were in large quantities, especially phosphorus content of those was highest ammount for the all samples. 2. Sodium content was much ammount in the Lentinus edodes (39mg) and Ganoderma lucidurn (20 mg), Culturing in wood and soil, while potassium was very high ammount in the Aloe arborescens and other samples. Mush ammount of magnesium as compared with others was Lentinus edodes (144mg), Ganoderma lucidurn (128mg), Aloe arborescerts (50mg) and pleurotus ostreatus (60mg). Phosphorus content of Ganoderma lucidurn, Lentinus edodes, Gyrophora esculenta, Auricularia polytricha and Agaricus bisporus was much ammount while iron content of all samples equality higher ammount. Sodium content of Aloe arborescens was not analyzed out for almost all, its potassium (82mg), magnesium (50mg) and iron (18rng) content comparatively higher quentity than others minerals and phosphorus volume (4.9mg) as compared with others, was conspicuously lower detect. 4. Cadimium and lead content of harmful metal element were detected on trace quentity for the most part samples 5. Organic acids of samples i.e., Legtinus edodes, Agaricus bisporus, Pleurotus ostreatus and Ganoderma lucidum were Citrate, Malate, Fumalate, Succinate, Oxalate, Acetate, Lactate, and Tartarate and Citrate, Malate and Fumarate contents were higher amount remarkbly than other organic acids. Tartarate content was trace amount.

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Microscopical observation and randomly amplified polymorphic DNA (RAPD) analysis of artificially cultivated Ganoderma applanatum

  • Woo-Sik Jo;Young-Hyun Rew;Seung-Chun Park
    • 한국버섯학회지
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    • 제22권2호
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    • pp.48-52
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    • 2024
  • This study investigated the microscopic characteristics and genetic relationships of Ganoderma applanatum fruiting bodies. Basidiospores were brown, ellipsoid, and had one or two large vacuoles and a double wall. The surface of basidiospores was smooth or wrinkled and most had numerous small and shallow holes. The length and width of basidiospores of Ganoderma applanatum isolates GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823, and ASI 53399 were on average 7.6×4.8 ㎛, 7.9×4.6 ㎛, 7.7×4.9 ㎛, 8.2×5.3 ㎛, 7.7×5.0 ㎛, 8.0×4.9 ㎛, and 7.9×4.9 mm, respectively. In contrast, the basidiospores of Ganoderma lucidum isolate ASI 7125 were 7.7×5.2 ㎛. Using the universal ITS1/ITS4 primer set, the ITS region of the isolates were amplified and sequenced. The ITS sequences were very closely related to G. applantum isolate GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823 and ASI 53399, but were not the same species. Whereas, G. lucidum isolate ASI 7125 belongs to different group.

Antiallergic effect of ginseng fermented with Ganoderma lucidum

  • Bae, Eun-Ah;Trinh, Hien-Trung;Rhee, Young-Kyung;Lee, Young-Chul;Kim, Dong-Hyun
    • Journal of Ginseng Research
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    • 제32권1호
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    • pp.57-61
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    • 2008
  • Ginseng (the root of Panax ginseng CA Meyer, family Araliacease), which is used in Korea, China and Japan as a herbal medicine, was fermented with Ganoderma lucidum (GL) and their antiallergic effects were investigated. Of GLs used for fermentation, KCTC 6283 potently produced ginsenoside Rh2, followed by KFRI M101. KCTC 6532, and ginsenoside Rd, followed by KFRI M101. Oral administration of these fermented ginseng extracts inhibited allergic reactions, passive cutaneous anaphylaxis reaction induced by IgE and scratching behaviors induced by compound 48/80. Of them, the ginseng extract fermented by KCTC 6532 and KFRI M101 potently inhibited allergic reactions compared to that fermented by KCTC 6283. These findings suggest that the fermentation of ginseng with GL can increase its antiallergic activity and the increment of its antiallergic effect may be due to the biotransformation of ginseng saponins to ginsenosides Rd and Rh2.

영지버섯 생장점 단백다당체 GLB의 대식세포 활성화 효과 (Activation of Macrophages by GLB, a Protein-polysaccharide of the Growing Tips of Ganoderma Lucidum)

  • 오정연;조경주;정수현;김진향;;정경수
    • 약학회지
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    • 제42권3호
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    • pp.302-306
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    • 1998
  • In the previous study we described the antitumor activity of GLB, a protein-polysaccharide fraction of the growing tips of Ganoderma lucidum, against sarcoma 180 solid tu mor in ICR mice. In this study we investigated the stimulatory activity of GLB on macrophages. When analyzed using a flow cytometer, GLB ($100{\mu}g/ml$) was found to increase the phagocytic activity of the BALB/c mouse peritoneal macrophages as well as chicken macrophage BM2CL cells against FITC-labeled C.albicans by 55.2% and 21.2%, respectively. GLB also increased the spreading and the expression of MHC class II molecules of BM2CL cells as well as the mouse peritoneal macrophages. From these results, it is clear that GLB is a strong stimulator to the macrophages.

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Evaluation of mycelial growth of Lentinula edodes and Ganoderma lucidum originated from South Korea and Brazil

  • Motta, Marcia Araujo;Jang, Jeonghwa;Kim, Mijeong;Choi, Eunsil;Kim, Jiyoung;Torracca-Rocha, Anna Carolina;Kong, Won-Sik;Urban, Arailde Fontes
    • 한국버섯학회지
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    • 제15권2호
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    • pp.94-97
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    • 2017
  • The choice of strain, substrate, and environmental conditions are fundamental factors that determine the ability of a fungus to develop and produce mushrooms with high quality. The objective of this study was to compare the strains of Lentinula edodes and Ganoderma lucidum originated from South Korea and Brazil in relation to their vegetative development in different culture media, to obtain isolates with high quality. The strains of the two mushroom species from Brazil and South Korea grew slowly, possibly because of abiotic factors or storage technique used. However, it may be concluded that temperature, humidity, light, pH, and nutrients from the substrate, if not regulated appropriately may affect the vegetative species.

Cytotoxic Triterpenoids from the Fruiting Bodies of Ganoderma lucidum

  • Tung, Nguyen The;Trang, Tran Thi Thu;Cuong, To Dao;Thu, Nguyen Van;Woo, Mi Hee;Min, Byung Sun
    • Natural Product Sciences
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    • 제20권1호
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    • pp.7-12
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    • 2014
  • Twelve triterpenoids (1 - 12) were isolated from $CHCl_3$-soluble fraction of fruiting bodies of Ganoderma lucidum. Extensive spectroscopic and chemical studies established the structures of these compounds as butyl lucidenate P (1), butyl lucidenate $E_2$ (2), butyl lucidenate $D_2$ (3), butyl lucidenate Q (4), ganoderiol F (5), methyl ganoderate H (6), methyl ganoderate J (7), lucidumol B (8), ganodermanondiol (9), methyl lucidenate N (10), methyl lucidenate A (11) and butyl lucidenate N (12). All of the compounds were examined for their cytotoxic activity against HL-60, HeLa, and MCF-7 cancer cell lines. Among them, compounds 4 and 8 showed cytotoxic activity with $IC_{50}$ values of 6.6 and 1.6 ${\mu}M$ against HL-60, respectively. In addition, compound 8 also showed cytotoxic activity with $IC_{50}$ values of 2.0 ${\mu}M$ against HeLa cancer cell line, other compounds were moderate or inactive.

Hypoglycemic Effect of Exo- and Endo-biopolymers Produced by Submerged Mycelial Culture of Ganoderma lucidum in Streptozotocin-Induced Diabetic Rats

  • Yang, Byung-Keun;Michael A Wilson;Cho, Kai-Yip;Song, Chi-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제14권5호
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    • pp.972-977
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    • 2004
  • The hypoglycemic effect of an exo-biopolymer (EXO) and endo-biopolymer (ENDO) produced from submerged mycelial culture of Ganoderma lucidum was investigated in streptozotocin (STZ)-induced diabetic rats. Both the EXO and ENDO showed hypoglycemic potential, however, the former proved to be more potent than the latter. The administration of the EXO at the dose of 100 mg/kg body weight (BW) significantly reduced the plasma glucose level (23.5%) and increased the plasma insulin level (2.2 fold) in the diabetic animals. The EXO also lowered the plasma total cholesterol, triglyceride, low-density lipoprotein (LDL) cholesterol, and athrogenic index by 14.7, 31.4, 24.1, and 45.4%, respectively, and reduced the liver total cholesterol and triglyceride levels by 6.7 and 25.8%, respectively. It increased the plasma high-density lipoprotein (HDL) cholesterol (37.7%), compared to the control group. Furthermore, the alanine transaminase (ALT) and aspartate transaminase (AST) showed lower activities in the EXO administered groups than the other experimental groups. Taken together, these results suggest that the exo-biopolymer may alleviate the blood glucose level by increased insulin secretion.

영지 에탄올 추출물의 마우스 유래 뇌 해마세포 보호효과 (Neuroprotective Effects of Ethanol Extract of Ganoderma lucidum L. on murine hippocampal cells)

  • 이승철;임남경;정혜영;최은화;전수명;정길생
    • 생약학회지
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    • 제45권2호
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    • pp.161-167
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    • 2014
  • Ganoderma lucidum L. (GL) is a traditional oriental medicine that has been widely used as anti-inflammatory, antitumor and anti-oxidant in Korea and other Asian countries. In this study, we investigated the ethanol extract of GL has neuroprotective effects in murine hippocampal HT22 cells. GL ethanol extract has the potent neuroprotective effects on glutamate-intoxicated cells by inducing the expression of heme oxygenase (HO)-1 in HT22 cells. GL ethanol extract increased JNK phosphorylation. Obviously, When we treated the GL extract with c-Jun N-terminal kinase (JNK) inhibitor (SP600125), HO-1 expression was reduced. Moreover, we found that GL treatment caused the nuclear accumulation of Nrf2. In conclusion, the ethanol extract of GL significantly protects glutamate-induced oxidative damage by induction of HO-1 via Nrf2, JNK pathway in mouse hippocampal HT22. These results suggest that GL ethanol extract would be a good source for taking active compounds and may be a potential pharmaceutical products for brain disorder induced by neuronal damage and oxidative stress.