• 수산해양기술연구
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• 제50권2호
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• pp.154-161
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• 2014

Maturity and spawning of the giant Pacific octopus, Octopus dofleini was investigated based on the samples collected in the coast of Gangwondo, East Sea, from January to December in 2009. Individuals of mature stage had a higher occurrence rate from February to May, and gonadosomatic index (GSI) also showed a higher value from February to May than the other months. For that reason, the spawning period was estimated from February to May with the main spawning period from March to May. The total number of eggs in the fecundity (F) ranged from 9,045 in 11.0 kg (TW) to 148,682 in 16.5 kg (TW), which was proportional to total weight (TW), and the relationship between total weight and fecundity was $F=0.185{\times}TW^{2.238}$ ($R^2=0.389$). The total weight at 50% group maturity (TW0.5) of female was estimated to be 12.2 kg.

• 가정∙방문간호학회지
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• 제13권1호
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• pp.46-53
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• 2006

Chunchoen Community Home Nursing Program (CCHNP) sponsored by Chuncheon City and Gangwondo Nurses Association was the first trial of community based home health nursing in Korea. The services have started since 1996. The purpose of the study was the evaluation CCHNP to establish standard of community based nursing program in Korea. The methods of evaluation were quantitative and Qualitative approaches. Evaluation for organization and management were conducted by the American Community Health Accreditation Program Standards With the result. several recommendations were suggested : It is necessary to expand the program to all the Gangwondo cities. And the liaison system is necessary between medical care facilities and community nursing home agency to establish the continuity of health care delivery system in home nursing program. Also it is necessary to set up the supporting system between community home nursing care agency and hospital home nursing department. Finally, the National Health Insurance should cover the cost of community home nursing care.

• 한국방재학회 논문집
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• 제2권1호
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• pp.161-167
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• 2002

전국단위로 시행되고 있는 산불위험시기가 전국 9개 지역(강원, 경기, 경남, 경북, 전남, 전북, 제주, 충남, 충북)과 차이가 있는지를 알아보기 위하여 산불발생건수와 피해면적을 가지고 순기별로 구분하여 상관분석을 실시하였다. 분석 결과 산불발생건수에서는 모든 지역에서 유의성이 있는 것으로 나타났고, 산불피해면적에서는 경남, 제주와 유의성이 없는 것으로 나타났다. 이에 전국을 대상으로 모든 지역을 설명하기에는 부족하므로 전국 9개도를 대상으로 하여 산불위험시기를 구분하기 위하여 산불발생과 피해면적을 이용하여 분산분석의 다중검정을 실시하고 순기별로 유의성 검정을 실시하였다. 그 결과 산불발생에 있어서 제주를 제외한 지역에서 1% 유의성이 있었고, 산불피해면적은 강원, 제주를 제외한 지역에서 1% 유의성을 보였다. 각 지역별로 위험시기를 구분할 수 있지만 행정적 효율성을 높이기 위하여 지역별 상관분석 결과를 이용하여 산불위험시기가 비슷한 중부권(강원, 경기, 경북, 전북, 충남, 충북), 남부권(경남, 전남), 그리고 제주권(제주)으로 분류하였다. 산불발생시기에 있어서는 중부권은 2월중순${\sim}$5월상순까지 전체의 81%가 발생한 반면 남부권은 1월하순${\sim}$4월중순까지 전체의 71%가 발생하였다. 산불피해면적에 있어서는 중부권은 2월중순${\sim}$5월상순까지 전체의 98% 가 발생한 반면 남부권은 1월하순${\sim}$4월중순까지 전체의 82%가 발생하였다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.169-175
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• 2013

Recently, the transgenic animal production technique is very important for the production of bio-parmaceutical as animal bio-reactor system. However, the absence of survival evaluation in vitro produced transgenic embryos has been a problem of the low productivity of transgenic animal because of absent of pre-estimate of pregnancy after transgenic embryos transferred into recipient. Therefore, this study is conducted to improve efficiency of transgenic cattle production by improving the non-surgical embryo transfer (ET) method. Transgenic bovine embryos were produced by injection of feline immunodeficiency virus enhanced green fluorescent protein (FIV-EGFP) lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization (IVF) was occured. Normal IVF and EGFP expressing blastocysts were transferred into recipients. Results indicated that 2 expanded blastocysts (34.7%) transferred group showed significantly (P<0.05) higher pregnancy rate than 1 expanded blastocyst (26.8%) transferred group. In case of parity of recipient, ET to heifer (34.9%) showed significantly (P<0.05) higher pregnancy rate than ET to multiparous recipient (21.2%). However, there are no significant differences of pregnancy rate between natural induced estrus and artificial induced estrus groups. Significantly (P<0.05) higher pregnancy rate was obtained from recipient group which have normal corpus luteum with crown group (34.8%) than normal corpus luteum without crown (13.6%). Additionally, treatment of $100{\mu}g$ Gn-RH injection to recipient group (38.6%) 1 day before ET significantly (P<0.05) increase pregnancy rate than non- Gn-RH injection to recipient group (38.6%). We also transferred 2 EGFP expressing expanded blastocysts to each 19 recipients, 7 recipients were pregnant and finally 5 EGFP transgenic cattle were produced under described ET condition. Therefore, our result suggested that transfer of 2 good-quality expanded blastocysts to $100{\mu}g$ of Gn-RH injected recipient which have normal corpus luteum with crown is feasible to produce transgenic cattle.

• 한국수정란이식학회지
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• 제28권3호
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• pp.185-191
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• 2013

The purpose of this study is to improve production efficiency of vitrified-thawed transgenic bovine embryos. Transgenic bovine embryos were produced by injection of FIV-GFP lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization. EGFP-expressing transgenic bovine blastocysts were cultured in serum-containing and serum-free medium. These blsatocysts were vitrified by pull and cut (PNC) container made with 0.25 cm plastic straw. Results indicate that total developmental rates of normal IVF embryo cultured in serum-containing and-free medium into blastocyst were not significantly different (22.3 vs 21.5%) and those of GFP-expressing transgenic bovine embryo into blastocyst showed no significant difference between serum-containing (13.9%) and-free medium (13.1%). However, developmental rate of GFP transgenic embryo was significantly (P<0.05) lower than its of normal IVF embryos. In additional study, we vitrified GFP transgenic normal bovine blastocysts using PNC vitrification method. Survival rate of vitrified-thawed GFP transgenic blastocyst (23.1%) was significantly (P<0.05) lower than its of normal blastocysts (68.9%). Although, survival rate of vitrified-thawed GFP transgenic blastocyst was lower than its of normal blastocyst, our result may suggested that PNC vitrification method is feasible to cryopreserve transgenic embryos. Our next plan will be the production of GFP express transgenic bovine derived from vitrified-thawed embryos using PNC method.

• 한국토양비료학회지
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• 제40권1호
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• pp.4-11
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• 2007

시설하우스 토양의 염류 집적은 연작장해로 인한 작물의 수량성 감소뿐만 아니라 지하수 등 수계의 오염 가능성을 높이고 있다. 본 연구는 이러한 시설하우스 토양의 염류집적 양상을 개선하는 한 방법을 제시하고자 시행되었다. 볏짚 (RS)과 파쇄목 (W)의 시용으로 토마토의 수량은 높아졌고 배꼽 썩음과의 발생은 줄었으며, 배추의 수량도 표준시비했을 때보다 증가되었다. 토양에 잔류하는 질산과 인산, 카리의 함량 및 전기전도도가 표준시비 처리구보다 낮았는데, 이는 토양검정을 기준으로 한 비료 시용량 절감도 한 이유일 것이다. 또한, 볏짚과 파쇄목의 시용은 토양 가비중을 낮춰 공극량을 높였으며, 토양 미생물체 탄소와 dehydrogenase 활성을 증가시켰다. 파쇄목의 시용은 볏짚과 비교하여 토마토와 배추의 수량이 높았으며, 작물 수확 후 토양에 질산과 인산이 덜 잔류하였고, 토양 미생물체와 dehydrogenase 활성은 높았다. 본 연구는 파쇄목 시용과 검정시비에 의한 화학비료의 절감이 하우스토양의 염류집적을 경감시킬 수 있는 가능성을 제시하였으며, 토양의 화학성뿐만 아니라 물리성과 미생물성도 함께 검토하면 토양 질의 변화에 미치는 영향을 평가하는 데 도움이 됨을 보여주었다.

• 환경생물
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• 제33권2호
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• pp.197-200
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• 2015

Asteroid specimens were collected from Shinnam, Gangwondo in the East Sea of Korea with fishing nets on 12 September 2014. The specimens were identified as Henricia reticulata Hayashi, 1940, belonging to family Echinasteridea of order Spinulosida. This species can be distinguished by a larger disc and broader arms compared to those other Henricia species. The morphological characteristics of this species are re-described with illustrations. By previous work of this genus, six species have been reported in the Korean fauna.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 추계학술대회
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• pp.277-277
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• 2017

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.54.1-54.1
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• 2005

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.210-211
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• 2008