• Title/Summary/Keyword: Gan

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Analysis of Microbial Community Structure in Soil and Crop Root System I. Analysis of Bacterial Community Structure in the Soil and Root System of Red Pepper and Tomato (토양과 작물근계의 미생물군집 구조해석 I. 고추 및 토마토 재배지 토양과 근계의 세균군집 구조해석)

  • Kim, Jong-Shik;Kwon, Soon-Wo;Lee, Seon-Ju;Jung, Beung-Gan;Song, Jae-Kyeong;Go, Soong-Ju;Ryu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.3
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    • pp.319-325
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    • 1999
  • A culture-dependent survey of bacterial community in the soil-root system of red pepper and tomato was conducted by dilution plate count method. The bacterial community within soil was not different from that of rhizoplane. However, the populations of fluorescent, pseudomonads were higher in rhizoplanes than in soils and higher in healthy rhizoplanes than in Phytophthora disease-infested rhizoplanes. The bacterial community of the pepper cropped soil and rhizoplanes was very similar to that of the tomato-cropped soil and rhizoplanes. Among 285 identified bacterial colonies, most colonies were belong to two groups by fatty acid analyses: 52% of the 285 colonies were belong to low G + C gram positive bacteria group. Bacillus spp. and 33% were belong to high G + C gram positive bacteria group. In order to use beneficial microorganisms to agro-ecosystem, these data of field trials should be intensively accumulated.

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Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds

  • Li, Jiao;Zhang, Lu-Pei;Gan, Qian-Fu;Li, Jun-Ya;Gao, Hui-Jiang;Yuan, Zheng-Rong;Gao, Xue;Chen, Jin-Bao;Xu, Shang-Zhong
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.11
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    • pp.1405-1411
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    • 2010
  • Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

DNA and Proteomic Analysis of Ginseng Radix Rubra Herbal-acupuncture Solution(GRR-HAS) on Gene Expression in HepG2 Carcinomar Cells (홍삼약침액(紅蔘藥鍼液)의 DNA와 단백질 발현(發顯)에 미치는 영향(影響))

  • Won, Eun-Ju;Lee, Bong-Hyo;Lim, Seong-Chul;Jung, Tae-Young;Seo, Jung-Chul;Lee, Kyung-Min
    • Journal of Acupuncture Research
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    • v.23 no.3
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    • pp.177-190
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    • 2006
  • Objectives : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cell lines. Oligonucleotide microarray and proteomic approaches were employed to screen the differential expression genes. Methods : GRR~HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS (0.1, 0.5, 1.5, 10, $20mg/m{\ell}$) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with $1.5mg/m{\ell}$ of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : It has no cytotoxic effects on both HepG2 cells in all concentrations(0.1, 0.5, 1.5, 10,$20mg/m{\ell}$). In oligonucleotide microarray assay, the number of more than twofold differentially regulated known genes was 320 with 6 up-regulated and 314 down-regulated genes in HepG2 cells. In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. One down -regulated protein was protein disulfide isomerase and up-regulated proteins were fatty acid binding protein 1 and 14-3-3 gan1lTIa protein by $1.5mg/m{\ell}$ of CRR-HAS. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.

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A Study of CNN-based Super-Resolution Method for Remote Sensing Image (원격 탐사 영상을 활용한 CNN 기반의 초해상화 기법 연구)

  • Choi, Yeonju;Kim, Minsik;Kim, Yongwoo;Han, Sanghyuck
    • Korean Journal of Remote Sensing
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    • v.36 no.3
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    • pp.449-460
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    • 2020
  • Super-resolution is a technique used to reconstruct an image with low-resolution into that of high-resolution. Recently, deep-learning based super resolution has become the mainstream, and applications of these methods are widely used in the remote sensing field. In this paper, we propose a super-resolution method based on the deep back-projection network model to improve the satellite image resolution by the factor of four. In the process, we customized the loss function with the edge loss to result in a more detailed feature of the boundary of each object and to improve the stability of the model training using generative adversarial network based on Wasserstein distance loss. Also, we have applied the detail preserving image down-scaling method to enhance the naturalness of the training output. Finally, by including the modified-residual learning with a panchromatic feature in the final step of the training process. Our proposed method is able to reconstruct fine features and high frequency information. Comparing the results of our method with that of the others, we propose that the super-resolution method improves the sharpness and the clarity of WorldView-3 and KOMPSAT-2 images.

Characteristics of Atmospheric Speciated Gaseous Mercury in Chuncheon, Korea (춘천시 대기 중 가스상 수은 종 농도 특성에 관한 연구)

  • Gan, Sun-Yeong;Yi, Seung-Muk;Han, Young-Ji
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.5
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    • pp.382-391
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    • 2009
  • Atmospheric speciated mercury concentrations including total gaseous mercury (TGM) and reactive gaseous mercury (RGM) were measured in Chuncheon from March 2006 to November 2008. Average concentrations were 2.10 ${\pm}$ 1.50 ng/$m^3$ and 3.00 ${\pm}$ 3.14 pg/$m^3$ for TGM and RGM, respectively. RGM concentrations were higher during daytime than nighttime probably because of high photochemical activities. We found that RGM concentration considerably increased as ozone increased when fog occurred, indicating that ozone was the important oxidant for $Hg^0$ in aqueous phase. TGM concentration showed positive correlations with CO and $PM_{10}$ which can transport in long-range, but there was no correlation with $NO_2$. Considering that major source of mercury is combustion process, this result showed that local sources did not significantly impact on TGM concentration in Chuncheon. Five-day backward trajectories were calculated for the samples representing high and low concentrations of TGM, and determined that industrialized area of China including Shenyang and Beijing influenced TGM concentrations in Chuncheon.

Dependence of 0.01M CaCl2 Soluble Phosphorus on Extractable P and P Sorptivity in Upland Soil (밭토양(土壤)에서 유효린산함량(有效燐酸含量)과 인산흡수능(燐酸吸收能)에 따른 0.01M CaCl2 가용(可溶) 인산농도(燐酸濃度) 변화(變化))

  • Yoon, Jung-Hui;Jung, Beung-Gan;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.266-270
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    • 1998
  • The identification of soil P level that exceed crop requirement is a prerequisite in implementing sustainable management of fertilizer and manure P to prevent soil and freshwater from contamination. To investigate the relationship between 0.01M $CaCl_2$ soluble P, and available P and P sorption capacity of 40 soils, P content and P sorptivity were analyzed. Single linear relationship revealed the dependence of 0.01M $CaCl_2-P$ on available P($r^2=0.479$), bioavailable P($r^2=0.281$), P sorption($r^2=-0.465$) and P absorption coefficient($r^2=-0.056^{NS}$). Thus available P as $P_2O_5$(AVP) and P sorption (PS) were most important factors in determining the concentration of 0.01M $CaC1_2-P$($CaC1_2-P$). In multinomial equation related $CaC1_2-P$ with AVP and PS, the determination coefficient was improved to 0.745. The logarithm of $CaC1_2-P$ was linearly related to AVP/PS. Consequently, the equation, $0.01M\;CaCl_2-P=0.1284e^{0.3288AVP/PS}$ could be suggested to estimate the concentration of P in 20mL of 0.01M $CaCl_2$ solution containing 2g of soil shaken for 17 hours.

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Monitoring on Chemical Properties of Bench Marked Paddy Soils in Korea (우리나라 논토양(土壤)의 화학적(化學的) 특성(特性) 분석(分析))

  • Jung, Beung-Gan;Jo, Guk-Hyun;Yun, Eul-Soo;Yoon, Jung-Hui;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.246-252
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    • 1998
  • A survey was conducted to investigate the status of soils involving pH, electrical conductivity, total organic matter content, available phosphate and major exchangeable cations in the paddy soils sampled from 1,168 sites throughout the country. The content of soil chemical properties was lower on the average than the optimum levels for cropping. An average value showed pH 5.6, organic matter $25g\;kg^{-1}$, available phosphate $128mg\;kg^{-1}$, available silicate $72mg\;kg^{-1}$, and exchangeable potassium and the calcium and magnesium were 0.32, 4.0, $1.2cmol^+\;kg^{-1}$, respectively. Soil chemical properties was related with topography except soil pH. A soil pH and organic matter, available phosphate, exchangeable potassium increased with time while exchangeable calcium, magnesium available silicate decreased with time.

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Characteristics of selective area growth of GaN/AlGaN double heterostructure grown by hydride vapor phase epitaxy on r-plane sapphire substrate (HVPE 방법에 의해 r-plane 사파이어 기판 위의 선택 성장된 GaN/AlGaN 이종 접합구조의 특성)

  • Hong, S.H.;Jeon, H.S.;Han, Y.H.;Kim, E.J.;Lee, A.R.;Kim, K.H.;Hwang, S.L.;Ha, H.;Ahn, H.S.;Yang, M.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.19 no.1
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    • pp.6-10
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    • 2009
  • In this paper, a selective area growth (SAG) of a GaN/AlGaN double heterostructure (DH) has been performed on r-plane sapphire substrate by using the mixed-source hydride vapor phase epitaxy (HVPE) with multi-sliding boat system. The SAG-GaN/AlGaN DH consists of GaN buffer layer, Te-doped AlGaN n-cladding layer, GaN active layer, Mg-doped AlGaN p-cladding layer, and Mg-doped GaN p-capping layer. The electroluminescence (EL) characteristics show an emission peak of wavelength, 439 nm with a full width at half maximum (FWHM) of approximately 0.64 eV at 20 mA. The I-V measurements show that the turn-on voltage of the SAG-GaN/AlGaN DH is 3.4 V at room temperature. We found that the mixed-source HVPE method with a multi-sliding boat system was one of promising growth methods for III-Nitride LEDs.

Study on 『BianZhengGiMun)』's medical characteristics - In view of ShangHan - (『변증기문(辨證奇聞)』의 의학적(醫學的) 특징(特徵)에 관(關)한 고찰(考察) -상한문(傷寒門)을 중심(中心)으로-)

  • Lee, Won-Suk;Park, Sun-Dong;Park, Won-Hwan;Kim, Jun-Ki;Kim, Jong-Dae
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.2
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    • pp.33-85
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    • 1998
  • In order to find the characteristic medical methods of "BianZhengGiMun" by the author ChenShihTo, we have translated and analyzed the mentioned book. Upon analyses, the following facts were noted : 1) "BianZhengGiMun" is written and composed of case studies. Prescriptive methods described here differ rather from previously known methods, where as our nation's Oriental medicine practitioners refer and consider these methods as miraculous modern methodologies. 2) Book's content has much similarities to "ShangHanLun"'s original text. Moreover, the content is adjusted towards presenting methodologies not coverd in "ShangHanLun"'s text, together with adding more information to conserve "ShangHanLun"'s methods as well as to critique them. 3) Diagnostic methods mainly follow the ZangFuBianZheng(臟腑辨證)'s pattern, where it's recorded information and theories are valid. 4) "BianZhengGiMun"'s proscriptive methods usually follow BuYinShengJin(補陰生津)'s form, where they were appropriate for that period and circumstances. 5) In consideration of all of the above factors, ChenShihTo grasped ShangHan and WenBing(acute febrile disease due to exogenous factor) as WaiGanReBing(外感熱病)'s double sided characteristics, where this corresponds with the current belief. It is believed that this in turn would provide much practical value to the present time.

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Electrochemical Properties of Current Collector in the All-vanadium Redox Flow Battery (바나듐 레독스-흐름 전지에서 집전체의 전기화학적 특성)

  • Hwang, Gan-Jin;Oh, Yong-Hwan;Ryu, Cheol-Hwi;Choi, Ho-Sang
    • Korean Chemical Engineering Research
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    • v.52 no.2
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    • pp.182-186
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    • 2014
  • Two commercial carbon plates were evaluated as a current collector (bipolar plate) in the all vanadium redox-flow battery (V-RFB). The performance properties of V-RFB were test in the current density of $60mA/cm^2$. The electromotive forces (OCV at SOC 100%) of V-RFB using A and B current collector were 1.47 V and 1.54 V. The cell resistance of V-RFB using A current collector was $4.44{\sim}5.00{\Omega}{\cdot}cm^2$ and $3.28{\sim}3.75{\Omega}{\cdot}cm^2$ for charge and discharge, respectively. The cell resistance of V-RFB using B current collector was $4.19{\sim}4.42{\Omega}{\cdot}cm^2$ and $4.71{\sim}5.49{\Omega}{\cdot}cm^2$ for charge and discharge, respectively. The performance of V-RFB using each current collector was evaluated. The performance of V-RFB using A current collector was 93.1%, 76.8% and 71.4% for average current efficiency, average voltage efficiency and average energy efficiency, respectively. The performance of V-RFB using B current collector was 96.4%, 73.6% and 71.0% for average current efficiency, average voltage efficiency and average energy efficiency, respectively.