• 한국식품저장유통학회지
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• 제14권1호
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• pp.94-99
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• 2007

Pichia anomala SKM-T와 Galactomyces geotrichum SJM-59의 첨가가 배추 김치 발효에 미치는 영향을 알아보기 위하여, 동결건조한 효모를 첨가하여 실온 ($20{\pm}2^{\circ}C$)에서 7일간 저장하면서 김치의 품질을 측정하였다. 대조구와 Galactomyces geotrichum SJM-59는 저장 3일째에 최적의 pH와 산도를 나타내었다. G. geotrichum SJM-59 첨가구에서는 저장 5일째까지 적정 pH와 산도를 유지하였다. p. anomala SKM-T 첨가구의 pH는 서서히 감소하여 전 실험 기간동안 대조구보다 유의적으로 높은 pH를 나타내었다. P. anomala SKM-T 첨가구의 산도는 저장 기간이 증가할수록 처처히 증가하였으나, 전 실험기간 동안 적정 산도를 유지하였고, 대조구에 비하여 높은 젖산균 수를 나타내었다. 대조구에서는 저장 3일째에 최고의 LA/TM 비를 나타내었으나, 효모첨가구에서는 저장 5일째에 최고의 LA/TM 비를 나타내었다. 저장 3일째의 관능검사 결과, 대조구와 G. geotrichum SJM-59 첨가구 간에는 유의적인 차이를 나타내지 않았다. 효모첨가구는 감칠맛에서 높은 점수를 얻었다. P. anomala SKM-T 첨가구는 대조구보다 우수한 질감 특성을 나타내었다. 이상의 결과를 종합해 볼 때, G. geotrichum SJM-59 또는 P. anomala SKM-T의 첨가는 배추김치의 발효 기간을 지연시키는 것으로 사료되었다.

• Food Science and Biotechnology
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• 제14권4호
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• pp.487-492
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• 2005

Simple competition plate bioassays of Pichia anomala SKM-T and Galactomyces geotrichum SJM-59 were conducted to evaluate their potential as biological control agents that inhibit growth of Botrytis cinerea during post-harvest storage of strawberries (Fragaria ${\times}$ ananassa Duche, Red-Pearl). Occurrence rates of fungi on the surface of yeast-treated strawberries were evaluated during storage at $4^{\circ}C$. P. anomala SKM-T and G. geotrichum SJM-59 showed antifungal activities on agar plate, and P. anomala SKM-T maintained its desirable antifungal activity on surface of strawberries and its physicochemical properties during storage. Sensory evaluation was based on kinesthetics and consumer acceptability. Due to its potential antifungal activity, P. anomala SKM-T could function as biological control agent against spoilage fungi during post-harvest storage of strawberries.

• Food Science and Biotechnology
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• 제16권2호
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• pp.193-197
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• 2007

In order to investigate the effects of Pichia farinosa SKM -1, Pichia anomala SKM-T, Galactomyces geotrichum SJM-59, and Saccharomyces cerevisiae on extending the shelf life of kkakdugi, 4 kinds of lyophilized yeasts adding kkakdugis were prepared and stored at $4^{\circ}C$ for 30 days. Except S. cerevisiae adding group, 3 kinds of yeast adding groups were maintained their desirable levels (ca. pH 4.2 and 0.6% acidity) during the fermentation. The hardness of yeast adding groups was higher than those of control during the experiments. The number of yeast and the ratio of lactic acid against to total bacteria in P. farinosa SKM-1, P. anomala SKM-T, and G geotrichum SJM-59 adding groups were lower than that of control and/or S. cerevisiae adding group. Based on acidity, kkakdugi made with P. farinosa SKM-1, P. anomala SKM-T, and G geotrichum SJM-59 remained edible about 10 days longer than the control product.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.800-808
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• 2003

Three strains of yeasts, Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59, produced volatile flavor compounds during fermentation. To investigate these volatile flavor compounds, the liquid culture broth of the three yeast strains were extracted with methylene chloride, and then GC and GC-MS analyses were conducted. Flavor analyses revealed that 5, 12, and 15 kinds of volatile compounds were isolated, and 4, 8, and 11 volatile flavor compounds were identified, respectively. Phenylethyl alcohol was identified with the common volatile flavor compound of Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59. 1H-indole-3-ethanol, a precursor of plant growth hormone, was identified from Pichia anomala SKM-T.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.22-28
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• 2004

Livestock industry requires alternatives of antibiotics to prevent environmental pollution and to maintain public health. We herein report on an effective method to reduce ammonia from livestock manure, and confirmed environmentally-friendly livestock production by adding three types of yeast probiotics, Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59, into the feed stuff, separately and/or mixed, and these three types of yeasts were administered to the Hy-line brown layers for 8 weeks. Compared with control, the laying performance, the egg quality, and the number of intestinal lactic acid producing bacteria of the treated group were improved and/or increased significantly. Pichia anomala SKM-T potently reduced ammonia production from poultry manure, and the other strains were also able to reduce the ammonia from it. The optimum condition for the reduction of ammonia with Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59 was obtained by using the augmented centroid-simplex design. The ratio of optimum condition was Pichia farinosa SKM-1:Pichia anomala SKM-T:Galactomyces geotrichum SJM-59=0.295:0.209:0.080, and the estimate was -123.36 (p=0.0l38). An ability to reduce the ammonia production from livestock manure was maintained at $30^{\circ}C$ for 15 weeks.

• Food Science and Biotechnology
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• 제14권4호
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• pp.493-497
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• 2005

Probiotics are generally excreted within a few days if their ingestion in feces at the same rate as or even more quickly than a transit marker (meaning not clear). Ability of probiotics to adhere to intestine prolongs their persistence in gastrointestinal tract, allowing them to exert healthful effects longer. Hydrophobicities, zeta potentials, Alcian blue-binding capacities, and sedimentation profiles of Pichia farinosa SKM-1, P. anomala SKM-T, and Galactomyces geotrichum SJM-59 were determined to evaluate characteristic properties of cell surfaces responsible for adhesion. Results of intestinal Caco-2 cell line in vitro and murine intestine in vivo studies revealed these strains exhibit adhesive properties regardless of their cell surface hydrophobicity.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 추계학술대회 및 정기총회
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• pp.152-152
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• 2003