• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.3
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• pp.239-247
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• 2018

Soybean produces three major types of isoflavones, daidzein, genistein, and glycitein aglycones and their glucosides and malonylglucosides. It has been known that malonylated glucosides are rapidly converted to their corresponding aglycones due to the unstable thermolabile glucoside malonates; therefore, the analytical study of malonylated glucosides has been insufficient. In this study, we analyzed the malonylglucoside content in soybean seeds. Isoflavone analysis of three soybean cultivars revealed that 81.5~90.0% of the total isoflavones were malonylglucosides, whereas aglycones were rarely detected. Moreover, the total isoflavone content increased during a 5-day germination period where growth regulators and coumaric acid treatments tended to yield higher isoflavone content than the normal germination treatment, however the differences were not significant; notably, the isoflavone accumulation trend continued with additional germination days. The content of malonylglucoside was higher than that of other isoflavones, which was 83.7~86.6% of the total isoflavone content in seeds with a 3-day germination period. Furthermore, isoflavones were significantly accumulated in the hypocotyl of seedlings with a 5-day germination period. The content of isoflavone in the hypocotyl of the Pungsannamul-kong was 10,240 ug/g when treated with coumaric acid, which was considerably higher than that of other cultivars and treatments. Additionally, soybean seeds heated at $60^{\circ}C$ for 1 hour produced higher isoflavone content than non-heated soybean seeds. Our results show that it is possible to increase the isoflavone content in soybean seeds through various treatments.

• Resources Recycling
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• v.24 no.4
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• pp.32-37
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• 2015

Cobalt is abundant only in some countries including Democratic Republic of the Congo. It would be necessary to secure overseas Co ores and Co extraction technology. Two kinds of matte varying the sulfur content were manufactured by smelting reduction of Co concentrate containing ~8 wt% Co, ~19 wt% Cu, and ~3 wt% Fe. The amount of Co, Cu and Fe was concentrated to 19~21 wt%, 39~41 wt%, and 7~9 wt% respectively in the resulting matte. High-pressure leaching of matte was performed in an autoclave with considering the effect of oxidizing agent, $H_2SO_4$ concentration as a lixiviant, and the amount of sulfur added to the matte. An oxidizing agent (oxygen) is necessary to improve Co leaching efficiency enabling usage of a dilute $H_2SO_4$ leaching agent. An increase in $H_2SO_4$ concentration prevents selective leaching of Co, and the sulfur content in matte has a minor influence on the Co leaching efficiency.

• Journal of Climate Change Research
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• v.5 no.4
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• pp.277-284
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• 2014

Biochar application to soil is widely known to have effects of climate change mitigation and soil quality improvement. However, effects of biochar on soil ecosystem are not always positive and some biochars are reported to contain toxic materials which might influence soil ecosystem. In this context, this study aims to investigate behavioral changes of earthworms(Eisenia fetida, Eisenia andrei) in response to different application rates of biochar to artificial soil. Treatment included two types of biochars made from rice husk (RH_Char) and wastewater sludge (SL_Char) with 1% and 10% application rates, respectively. Avoidance test revealed that earthworms did not avoid SL_Char treatments at 1% and 10%, while they rather moved to the RH_Char treatments probably due to higher labile carbon content(Hot water extractable carbon) of the RH_Char. The HWC content of RH_Char was 4 times higher than that of the SL_Char. Results of reproduction test showed that the survival rates, number of juveniles and number of cocoons were not influenced by biochar application except for the treatment of SL_Char at 10% rate. In the SL_Char 10% treatment, fatality was approximately 3.3 times as high as the control and the number of cocoons was 1.3 times higher in the same treatment than the control, indicating that earthworms were under environmental stress. The possible explanation for the stress condition was related to higher Cd, Ni, Cr, and As contents in the SL_Char. Overall results imply that biochar application at low rate might not change earthworms' behavior for the short term, while the reproduction behavior might be negatively influenced under the high application rate.

• Journal of Korea Entertainment Industry Association
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• v.14 no.7
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• pp.589-597
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• 2020

The purpose of this study was to verify the acne care improvement effects of natural cosmetics with distilled bamboo vinegar contents and develop the materials for acne cosmetics. For the research subjects, 20 teenage boys and girls were selected and based on random number table, 10 subjects were assigned in the control group that used natural cosmetics (foam cleanser, toner, facial pack) and 10 subjects were assigned in the experimental group that used natural cosmetics with distilled bamboo vinegar contents (foam cleanser, toner, facial pack). The natural cosmetics was used for 12 weeks, and Mark-·Vu facial analysis system was used to measure the sebum, pore size, and redness before using the natural cosmetics, 6 weeks after using the natural cosmetics, and 12 weeks after using the natural cosmetics. For the collected data, SPSS v. 21.0 statistics package program was used for the analysis, and the results are as follows. First, it is a safe natural cosmetics based on the results of the patch test to confirm the skin safety of the natural cosmetics. Second, homogeneity was secured based on the results of the test of homogeneity for the sebum, pore size, and redness of the control group and experimental group. Third, in the verification of acne skin improvement effects of natural cosmetics with distilled bamboo vinegar content, the experimental group had higher reduction rate of changes in the sebum, pore size, and redness on the forehead and right cheek compared to the control group. Therefore, it was identified that the natural cosmetics with distilled bamboo liquid content is safe for the skin and effective for reducing the sebum, pore size, and redness for acne skin. For this reason, it is anticipated for distilled bamboo vinegar to be used in the cosmetics industry.

• Journal of Life Science
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• v.24 no.10
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• pp.1102-1109
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• 2014

Chungkookjang has several functional properties, such as fibrinolytic activity, anticancer effects, and antioxidant effects. However, children do not like Chungkookjang because of its foul odor. A mixed culture of Bacillus subtilis MC31 and Lactobacillus sakei 383 was used to improve the production of GABA in Chungkookjang and its flavor. Most of the foul odor of Chungkookjang was removed. The slime content and viscosity of Chungkookjang fermented in the mixed culture were similar to those of commercial Chungkookjang when B. subtilis MC31 and Lactobacillus sakei 383 were inoculated in a 1:1 ratio. The maximum GABA content was obtained when Chungkookjang was fermented with B. subtilis MC31 and L. sakei 383, which was fermented at $37^{\circ}C$ for 72 hr. During the period of fermentation, the viable cell number of B. subtilis MC31 reached a peak (log 9.13 CFU/g) at six days, and L. sakei 383 reached a peak (log 6.78 CFU/g) at two days. The moisture, crude ash, crude protein, crude fat, and crude fiber contents were 61.71%, 2.05%, 17.54%, 8.36%, and 1.95%, respectively. The amino-type nitrogen content of Chungkookjang fermented by B. subtilis MC31 and L. sakei 383 was less than Chungkookjang fermented by B. subtilis MC31 alone. The ammonia-type nitrogen and reducing sugar content of the Chungkookjang fermented by B. subtilis MC31 and L. sakei 383 were higher than that of steamed soybean. The glutamic acid and GABA content detected with an amino acid analyzer were 1.40 mg/g and 0.47 mg/g, respectively. These results suggest that fermentation with B. subtilis MC31 and L. sakei 383 in a 1:1 ratio removes more of the foul odor and increases the GABA content compared with single fermentation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.684-696
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• 1995

A mutant for chalky endosperm and genetic male sterility (GMS) was newly developed in rice. The two characters were found to be controlled by single recessive gene which has pleiotropic effect, indicating that chalky seeds should be GMS seeds in segregating populations. Chalky seeds showed the same shape and size as normal seeds. However, starch composition of central part of endosperm was looser and shape of starch granules was rounder compared with normal endosperm, resulting in significantly lower grain weight, absolute density and grain hardness in chalky grains than in normal ones. Amylose content and gel consistency of chalky grains were much lower and harder, respectively. Male sterile plants showed much shorter plant height, poorer panicle exsertion and lesser panicle number compared with normal plants. Microspore abortion stage in pollen developmental process was observed as before meiosis. Male sterility of the mutant was stable regadless of temperature and day length. A system breeding hybrid rice using this mutant was discussed, comp ring with other systems utilizing cytoplasmic-genic male sterility(CGMS) and environment sensitive GMS(EGMS). Separation of GMS seeds in mixed seed bulks by specific gravity (1.14∼1.16g / cm3) was successful about 85∼90%. But some mixed normal plants were seemed to be easily removed by the apparent difference in growth characters at seedling stage. The highest natural outcrossing rate of this GMS line was as 17.3 % in a plot treated with 2-row pollinator, I-row GMS, and GA3 + cutting of flag leaf + pollen-scattering by rope.

• Food Science and Preservation
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• v.25 no.1
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• pp.7-18
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• 2018

In this study, soy-powder yogurt (SPY) with enhanced levels of ${\gamma}$-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and $491.25{\mu}g/g$ in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.1
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• pp.55-61
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• 1987

A series of laboratory experiments were carried out to find the effects of soil organic matter contents, soil temperature, pH values, kinds and amount of nitrogen fertilizers on the denitrification-$N_2O$ gas evolution-. The results obtained were summarized as follows: 1. Denitrification rate, amount of $N_2O$ gas evolution, was influenced the order of organic matter contents>soil temperature>pH values>kinds of N-fertilizer>levels of N-fertilizer. 2. The highest dentrification rate was observed in organic matter content of 3.0%, pH values at 6.0 with application of $KNO_3$ at levels of 20 mgN/100g soil. 3. For the evolution of I mole $N_2O$ gas, averaged carbon consumption was obtained as 0.5 mole in all these experiment condition. However, the highest carbon consumption rate was obtained in organic matter contents for 1.0% with application of $(NH_4)_2SO_4$ at levels of 10 mgN/100g soil (1.06 mole) while lowest carbon consumption rate was obtained in organic matter contents for 3.0% with application of $KNO_3$ at levels of 20 mgN/100g soil (0.13 mole). 4. According to Michaelis-Menten's equation, the V/2 values for evolution of $N_2O$ gas was estimated by progress curve. The results obtained was as 550 ug for $(NH_2)_2CO$ and 1100 ug $N_2O/100g$ soil by application of $KNO_3$ in organic matter contents of 1.0% soil. On the other hand, when the application $(NH_4)_2SO_4$ the V/2 values of $N_2O$ gas was obtained as the amount of 490 ug/100g soil while V/2 values of $N_2O$ gas by application of $KNO_3$ was on the linear line in soil organic matter contents of 3.0%.

• Journal of Korean Academy of Nursing
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• v.36 no.6
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• pp.950-958
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• 2006

Purpose: The purpose of this study was to develop a critical thinking disposition scale for nursing students. Method: The developmental process was construction of a conceptual framework, development of preliminary items, verification of content validity, development of secondary items, verification of construct validity and extraction of final items. The conceptual framework and first preliminary 60 items were obtained through a review of relevant literature and the development of critical disposition scales by 10 researchers who had been studying critical thinking for one year These items were reviewed by five specialists for content validity and finally 55 items were chosen. The data was collected from October 1 to 15, 2004 and was analyzed using factor analysis and Cronbach's $\alpha$ with the SPSS program. The subjects were composed of 560 Bachelor of Science nursing students from 8 nursing schools. Result: There were 35 final items which were sorted into 8 factors. The factors were identified as 'intellectual integrity(6 items)', 'creativity(4 items)', 'challenge(6 items)', 'open-mindedness(3 items)', 'prudence(4 items)', 'objectivity(4 items)', 'truth seeking(3 items)' and 'inquisitiveness(5 items)'. The cumulative percent of variance was 55.107%. The reliability of the scale, Cronbach's alpha was.892 and the factors' ranged from.562-.836. Conclusion: The result of this study could be used for measuring critical thinking dispositions of nursing students. However, for further validity and reliability, repeated research is necessary.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.168-177
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• 2017

Strain A28-5, which can degrade xylan and agar in solid medium, was isolated from a coastal seawater sample collected from Jeju Island, South Korea. This strain was found to be a gram-negative, $Na^+$-requiring bacterial strain with a polar flagellum for motility. Additionally, the strain was tolerant to antibiotics such as ampicillin and thiostrepton. The G+C content of the genome was 43.96% and menaquinone-7 was found to be the predominant quinone. Major fatty acids constituting the cell wall of the strain were $C_{16:1}$ ${\omega}7c/iso-C_{15:0}$ 2-OH (23.32%), $C_{16:0}$ (21.83%), and $C_{18:1}$ ${\omega}7c$ (17.98%). The 16S rRNA gene sequence of the strain showed the highest similarity (98.94%) to that of Catenovulum agarivorans YM01, which was demonstrated by constructing a neighbor-joining phylogenetic tree. A28-5 was identified as a novel species of the genus Catenovulum via DNA-DNA hybridization with Catenovulum agarivorans YM01, and thus was named as Catenovulum jejuensis A28-5. The formation of tetramers and hexamers of xylooligosaccharides and (neo)agarooligosaccharides, respectively, were confirmed by thin-layer chromatography analysis using an enzyme reaction solution containing xylan or agarose with two crude enzymes prepared from the liquid culture of the strain.