• Journal of Microbiology and Biotechnology
• /
• 제31권12호
• /
• pp.1684-1691
• /
• 2021

In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment was also investigated. The mackerel meat storage experiment demonstrated that production of trimethylamine (TMA) and volatile basic nitrogen (VBN), the main components of fishy smell, was significantly reduced on the 20th day of storage after the HHP treatment compared to the untreated mackerels. The results demonstrated that the increased ammonia nitrogen rates in the 2000, 3000, and 4000 bar, HHP-treated groups decreased by 23.8%, 23.8%, and 31.0%, respectively, compared to the untreated groups. The enzyme activity of crude urease was significantly reduced in the HHP-treated group compared to that in the untreated group. Measurement of the volatile organic compounds (VOCs) in mackerel meat during storage indicated that the content of ethanol, 2-butanone, 3-methylbutanal, and trans-2-pentenal, which are known to cause off-flavor due to spoilage, were significantly reduced by HHP treatment. Collectively, our results suggested that HHP treatment would be useful for inhibiting the activity of urease, thereby reducing the fishy smells from fish and shellfish.

• 한국전기전자재료학회논문지
• /
• 제31권5호
• /
• pp.324-329
• /
• 2018

Red phosphor in glasses (PiGs) for automotive light-emitting diode (LED) applications were fabricated with 620-nm $CaAlSiN_3:Eu^{2+}$ phosphor and Pb-free silicate glass. PiGs were synthesized and mounted on high-power blue LED to make a monochromatic red LED. PiGs were simple mixtures of red phosphor and transparent glass powder. After being fabricated with uniaxial press and CIP at 300 MPa for 20 min, the green bodies were thermally treated at $550^{\circ}C$ for 30 min to produce high dense PiGs. As the phosphor content increased, the density of the sintered body decreased and PiGs containing 30% phosphor had a full sintered density. Changes in photoluminescence spectra and color coordination were studied by varying the thickness of plates that were mounted after optical polishing. As a result of the optical spectrum and color coordinates, PiG plate with $210{\mu}m$ thickness showed a color purity of 99.7%. In order to evaluate the thermal stability, the thermal quenching characteristics were measured at temperatures of $30{\sim}150^{\circ}C$. The results showed that the red PIG plates were 30% more thermally stable compared to the AlGaInP red chip.

• 한국간호교육학회지
• /
• 제3권2호
• /
• pp.207-213
• /
• 1997

Basic science teaching and clinical education should be integrated whenever appropriate, and the development of skills, values, and attitudes which are emphasized to the same extent as the acquisition of knowledge in nursing. Problem-based learning provides a students-centered learning environment and encourages an inquisitive style of learning. The purpose of this paper is to review and comment the role of tutors and students on problem-based learning. The use of problem-based learning places a high demand on faculty members' time and support. The role of tutors in Problem-based learning focuses primarily on issues of developing and teaching the curriculum and on organizational implementation and institutionalization. Tutors are an integral part of course planning. Tutors serve as a constant source of feedback on student needs and concerns to the course director and constitute an informal steering committee while the course is in progress. Tutors write cases, develop student evaluation methods, recommend resources, suggest modifications in lectures and laboratories. Students have a limited amount of time available to study what is traditionally defined as the core content of nursing. But, the role of students in Problem-based learning would be active, independent learners and problem-solvers rather than passive recipients of information. Students using a deep level approach attempt to integrate what they learn with what they already know, to understand the meaning underlying the material to be learned, and to look for explanations rather than facts. Students are encouraged, with appropriate guidance, to define their own learning goals, to select appropriate experiences to achieve these goals, and to be responsible for assessing their own learning progress. Problem-based learning is more flexible and meaningful, by encouraging student interaction, and by having a better emotional climate than the conventional learning.

• 한국치위생학회지
• /
• 제20권1호
• /
• pp.29-39
• /
• 2020

Objectives: This study was to examine the association of sugar-containing snacking habits with dental caries and periodontal diseases. Methods: This study was conducted on 2,807 adults who answered both oral examination and food intake frequency survey in the data of 2015 Korea National Health and Nutrition Examination Survey. The intake frequency of sugary snacks was selected from 17 kind of snacks, which had high sugar content and could affect oral disease. We used the complex sample logistic regression using SPSS 23.0 confirmed the correlation of sugary snack intake frequency and oral diseases. Results: 16.9% of all adults had a sugary snack at least three times a day. 20.7% of dental caries patients and 25.8% of people with periodontal diseases consumed sugary snacks more than three times a day. Especially, periodontal diseases were 1.51 times higher(p=0.008) in three or more consuming snacks group than that who ingested sugary snacks less than six times a week, by correcting the general characteristics and oral health behaviors. Conclusions: Oral health in adults affects oral health in old age, so from a public health perspective, integrated intervention is required to manage the self-cultivation health and to create correct eating habits.

• Journal of Dairy Science and Biotechnology
• /
• 제37권4호
• /
• pp.213-222
• /
• 2019

Milk and dairy products are the high value foods for the elderly population. In particular, fermented milk is the best source of calcium for people in the specific age group of over 79 years. It provides a good source of protein. Regular exercise and active lifestyle are recommended to slow down the muscle loss. However, exercising without proper nutrient intake is simply not sufficient at this age. Milk and dairy products provide the iron and protein content required for effective exercise-assisted growth. Milk nutrients have the advantage of being produced in various food forms, such as liquid, semi-solid, and powder types. Fat-soluble vitamins such as retinol and vitamin K can be encapsulated using various technologies for milk and dairy products. Using the encapsulation method, spray drying and fluidized-bed coating have been used for adding the micro-nutrients to the food. Microencapsulation technology is being applied in case of the fermented dairy products too. In particular, various wall materials are being developed to enhance the viability of probiotics. In the near future, advanced high-efficiency technologies that can effectively nourish the dairy products with nutrients will be developed to produce targeted high-nutrition value food for the elderly.

• 한방비만학회지
• /
• 제14권1호
• /
• pp.1-12
• /
• 2014

Objectives: The purpose of this study is to analyse research trends about medical leech in the last 10 years. Methods: We searched the papers with key words of 'medical leech', '水蛭' in China Knowledge Resource Integrated Database (CNKI), Korean traditional knowledge portal, DBPIA, KISS. We classified the papers by year and content. Results: 1. Result of the classification on the basis of the research methods, 50 studies are clinical case reports, 301 studies are experimental paper, 599 studies are clinical trial study, 219 studies are literature review. 2. Result of the classification on the basis of the research topics, 454 studies are the effect of anticoagulant research, 84 studies are diabetes and renal disease research, 194 studies are pharmacological properties research, and 43 studies are effect of allergic and anti-inflammatory research. 3. The proportion of the coagulation effect study tends to decrease every year since 2006. 4. Leech research tends to decrease in last 3 years. 5. Recently, there are many research in psychiatry, orthopedic diseases and beauty. 6. There are 10 studies related to leech in Korean. Conclusions: The researches of medical leech are actively done. The diversity of subjects is getting wider and richer.

• 한국약용작물학회지
• /
• 제23권5호
• /
• pp.385-394
• /
• 2015

Background : Dibenzocyclooctadiene lignans are secondary metabolites present abundantly in the fruits belonging to the genus Schisandra. According to previous studies, Schisandra lignans exhibit anti-inflammatory, anti-cancer and anti-diabetic properties, as well as an inhibitory effect on platelet aggregation. Therefore, establishing the Korean "Omija" (Schisandra chinensis) as a lignan-rich source, in addition to identifying and quantifying the lignans, is extremely valuable. Methods and Results : Dibenzocyclooctadiene lignans were analyzed with liquid chromatography using diode array detection/mass spectrometry, from methanol extracts subsequently identified by a constructed chemical library of 50 lignans. A total of 27 components of lignan including gomisin S were identified, of which schisandrin, gomisin A, gomisin N, deoxyschisandrin, ${\gamma}$-schisandrin, and schisandrin C were identified as the major components in the Korean Omija, Schisandra chinensis. These compounds were divided into two groups, S-biphenyl and R-biphenyl based on the configurations of the stereoisomers structures with contents of 661.7 and 1350.1mg per 100 g dry weight, respectively. The total lignan content averaged 2011.4mg per 100 g dry weight, of which schisandrin and gomisin N comprised the majority (771.8 and 420.5mg per 100 g dry weight respectively). Conclusions : Lignans which are present in high quantities in the ripe fruit of Schisandra chinensis are important functional compounds that play a major role in the prevention and treatment of human diseases.

• 생물환경조절학회지
• /
• 제31권4호
• /
• pp.332-342
• /
• 2022

케일(Brassica oleracea var. acephala)은 필수 아미노산, 비타민, 미네랄과 같은 수많은 영양소를 함유하고 특히 글루코시놀레이트가 풍부하기 때문에 전 세계적으로 가장 많이 소비되는 잎 채소 중 하나이다. 그러나 케일 품종 간의 글루코시놀레이트 합성과 관련된 유전자 발현에 대한 연구는 미비한 실정이다. 본 연구에서는 전사체 및 대사체 분석을 사용하여 식물공장에서 재배된 녹색(만추 및 맛짱) 및 적색 케일 품종(적곱슬)을 포함한 3 가지 케일 품종에서 글루코시놀레이트를 조사하였다. 재배 후 6주된 녹색 케일 품종의 생육 및 발달이 적색 케일 품종에 비해 높았다. High-performance liquid chromatography (HPLC) 분석에서 7가지 글루코시놀레이트를 분석하였다; 만추 품종에서는 5종의 글루코시놀레이트가, 맛짱과 적곱슬 품종에서는 4종의 글루코시놀레이트가 분류되었다. Glucobrassicin은 3가지 케일 품종에서 가장 높은 글루코시놀레이트 였다. 총 글루코시놀레이트 함량은 적곱슬 품종에서 가장 높았다. 전사체 분석에서는 8개의 유전자가 글루코시놀레이트 합성에 관여됨을 확인할 수 있었다. 이러한 결과는 케일 품종에 따라 글루코시놀레이트 함량과 축적 패턴이 다르다는 것을 시사한다.

• 한국초등과학교육학회지:초등과학교육
• /
• 제39권4호
• /
• pp.475-493
• /
• 2020

In Korea, the lower elementary grades are applying a topic-oriented integrated curriculum. It causes a lack of science content and inquiry experience for young children. The purpose of this study is to understand the scientific experiences and perceptions of the lower elementary graders and to inquire about the meaning of those experiences in their science learning. Four students (male 2, female 2), their parents, and their first-year homeroom teachers participated in the study. The data collection was mainly conducted through in-depth interview of them. Results showed that the 'experiments' were the student's the main perception of science, and the epistemic emotion that students mainly feel about science was 'curiousness'. Since most experiences were dependent on non-formal educational experience, the parents were an important factor to determine their experiences about science. Various factors, such as students' scientific experiences, their epistemic emotions, and the parents, interacted to form children's perception of science. The positive perception of science affected the level of motivation and expectation for science learning as the third grade. It is suggested that improvement of curriculum and textbooks should emphasize scientific exploration for the lower graders of elementary school, which provides them meaningful scientific experience in school.

• Journal of Microbiology and Biotechnology
• /
• 제32권6호
• /
• pp.808-815
• /
• 2022

In this study, gamma-irradiated mackerel (Scomber japonicus) meat was stored in a refrigerator for 20 days to examine the physicochemical changes related to fishy smell. The effect of gamma irradiation on the inhibition of the activity of crude urease extracted from Vibrio parahaemolyticus was also evaluated. Increased levels of trimethylamine (TMA) and volatile basic nitrogen (VBN) content, which are the main components causing fishy smell, were significantly reduced by day 20 of storage after gamma irradiation, indicating that freshness was maintained during storage. The ammonia nitrogen contents of 3, 7, 10, and 20 kGy gamma-irradiated groups were significantly decreased by 6.5, 15.2, 17.4, and 23.9%, respectively, compared to non-irradiated groups on day 20 of storage. In addition, urease activity decreased in a gamma irradiation intensity-dependent manner. Volatile organic compounds (VOCs) were measured during the storage of gamma-irradiated mackerel meat. The contents of ethanol, 2-butanone, 3-methylbutanal, and trans-2-pentenal, which are known to cause off-flavors due to spoilage of fish, were significantly reduced by day 20 of storage. Therefore, gamma irradiation can be considered useful for inhibiting urease activity and reducing fishy smell during fish storage.