The effect of metolachlor [2-chloro-N-(2-ethyl 1-6-methylphenyl)-N-(2-methoxy-l-methylethyl)-acetamide] on starch, sugar and protein content in relation with ${\alpha}$-amylase activity, and its interaction with GA in the germinating stage of rice were determined. The distinctive phytotoxic symptom was the failure of the primary leaf of rice to break the coleoptile. An inhibitory effect of metolachlor was nullified by the external application of GA $10^{-3}$M. A significantly slower starch degradation accompanying the lower content of sugars was observed in the gain of the metolachlor treated seedlings. Further, the total ${\alpha}$-amylase activity was significantly lower in the grain of rice seedlings treated with metolachlor than that of the untreated one, and the lower total ${\alpha}$-amylase activity could be due to an inhibition of ${\alpha}$-amylase formation.
The present study was carried out to get the basic information for clarifying physiological mechanism of breaking dormancy and sprouting in Panax ginseng roots. Changes in Abscisic acid (ABA) content and Gibberellin (GA) activity were investigated in one-year-old root during stratification at 4$^{\circ}C$. 15$^{\circ}C$. and 15$^{\circ}C$ after 60day-treatment at 4$^{\circ}C$. Sprouting rate at 15$^{\circ}C$ was 35% in 30days storage at 4$^{\circ}C$ and 100% in longer than 60days, but there was no sprout in both the constant treatment at 4$^{\circ}C$ or 15$^{\circ}C$ regardless of the treatment period. The longer the period of low temperature treatment. number of days to the first and 50% sprouting was shortened, and number of days to 50% from first sprouting was also shortened. ABA content in the upper part of root(contained bud) was gradually increased at both 4$^{\circ}C$ and 15$^{\circ}C$ as the treatment period was extended. and the degree of increase was higher at 15$^{\circ}C$. In the lower part. it showed a slight increase at 15$^{\circ}C$. while showed little change at 4$^{\circ}C$ throughout the treatment period. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$, it was greatly increased in the upper part. while rather slightly decreased in the lower part of root. GA activity in the upper part was gradually decreased at both 4$^{\circ}C$ and 15$^{\circ}C$, and the degree of decrease was higher at 15$^{\circ}C$. In the lower part. it was similar tendency to those in the upper part. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$. it was remarkably increased in both the upper and lower part. The increase was great in the low Rf region, while the decrease appeared relatively in the high Rf region compared to those of 60day-treatment at 4$^{\circ}C$. The above results indicated that the breaking dormancy and sprouting of bud were closely associated with the degree of GA activities in response to temperature condition .during stratification rather than the direct effect associated with the changes in ABA content.
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
/
v.29
no.4
/
pp.14-23
/
2016
Objectives : One of major complications of diabetes, diabetic ulcer is also one of the main reasons for amputation, and the prevalence rate is 4-10%. Laser therapy is widely used for leg ulcer and diabetic ulcer, and it is known to improve wound epithelialization, cellular content, and collagen deposition. The purpose of this study is to investigate the properties of the laser and the spectrum of laser instruments for diabetic ulcer. Methods : We performed literature search using the PubMed, Cochrane, CINAHL and Web of science for the data in English. In addition, other databases were checked for different languages such as OASIS and NDSL for the literature in Korean, CNKI in Chinese, and CiNii and J-STAGE written in Japanese. We excluded all review article and experimental studies, and only clinical studies using laser or light emitting diode (LED) for diabetic ulcer were selected. Results : A total twenty papers were selected. Different light sources were used as follows: LED, HeNe, InGaAlP, GaAlAs, GaAs, CO2, and KTP. The number of LED studies was 9, and HeNe laser was 7, and InGaAlP and GaAlAs laser was 2, GaAs, CO2, and KTP laser was 1 for each. Various energy density of the clinical study were reported. Conclusions : It is suggested that to select appropriate laser type and give the adequate output power to treat diabetic ulcer. Further evaluation and research for the condition of laser therapy to treat diabetic ulcers are warranted.
Gibberellin (GA) treatment on fruit stalk was effective in fruit growth but reduced the shelf life of fruit. This study was carried out to improve the shelf life of GA treated fruit. Fruit characteristics were surveyed according to the treatment part and concentration of polyamine in 'Whangkeumbae', 'Yeongsanbae', and 'Chuwhangbae'. GA was treated on fruit stalk at 30 days after flowering and 100, 200, and 300 ppm of polyamine were sprayed on leaves of fruit cluster and canopy at 7 day before harvest. In GA treated fruit, fruit weight was heavier as $10\%$ and fruit hardness was lower as $0.3\~0.5\;kg$ than control in all cultivar, but sugar content and palatability were no difference in each treatment. In morphological characteristics, the distance from pith to epidermis of GA treated fruit was faraway as $42.0\%$. Fruit hardness and palatability of 'Whangkeumbae' was good in the leaves spray on the fruit stalk with 200 ppm and the leaves spray on the canopy with 500 ppm at 30 day after harvest. 'Yeongsanbae' and 'Chuwhangbae' showed the same to the result. So we knew that the effective treatment to improve the storagibility after GA treatment on fruit stalk was the fruit stalk leaves spray with 200 ppm because it needed small amount of polyamine.
Journal of the Korean Society of Food Science and Nutrition
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v.28
no.1
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pp.140-144
/
1999
The physicochemical and organoreptic characteristics of hamburger patties with 1~5% powder of Sargassum thunbergii(ST) or Gelidium amansii(GA) were investigated. The addition of seaweed powder resulted in significant changes of L, a and b values of the patty. Water content of the patty increased with GA but not changed with ST. As increasing the amount of the powder, more cooking yield was observed. Na was the most abundant mineral, followed by Ca, Mg, Fe and Zn. The hamburger patty with 3% GA showed similar sensory scores of color and overall acceptability to control.
Kim, Sang-Kuk;Han, Chae-Min;Shin, Jong-Hee;Kwon, Tae-Young
KOREAN JOURNAL OF CROP SCIENCE
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v.63
no.3
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pp.265-271
/
2018
We investigated the effect of paclobutrazol (PB) and prohexadione-Ca (PC) on seed yield, and the content of oils and endogenous gibberellin in flax grown in a greenhouse. Flax seedlings were cultivated in a greenhouse with average ambient temperature ranging from $35^{\circ}C$ to $38^{\circ}C$ during the growing period, and then PB and PC were applied on the leaves. The height of flax plants was reduced significantly by the foliar application of PB and PC. The highest ripening seed rate recorded was 94.5% with 2000 ppm PC; a 10.3% increase compared with that of the control. Both PB and PC markedly increased the fructification of flax seed. The application of two plant growth retardants resulted in higher seed production in flax seedlings grown in open field than in those grown in a greenhouse. The highest oil content was observed in flax treated with PC, whereas, the lowest oil content was observed in flax treated with PB. The oil content and ratio of unsaturated fatty acids were higher in flax seedlings grown in greenhouse than in those grown in open field. The level of two endogenous bioactive gibberellins ($GA_1$ and $GA_4$) in flax seeds decreased with the application of PB and PC. The flax plants treated with PB and PC presented higher secoisolariciresinol diglucoside content than that of the control. The optimal concentration of PC was found to be 2000 ppm. In conclusion, the foliar application of 2000 ppm PC might be useful in increasing oil content and seed production in flax plants.
Seo, Chang-Woo;Lee, Seok-Min;Kang, Sang-Mo;Park, Yeon-Gyeong;Kim, Ah-Yeong;Park, Hyeon-Jin;Kim, Yoonha;Lee, In-Jung
KOREAN JOURNAL OF CROP SCIENCE
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v.62
no.4
/
pp.325-332
/
2017
This research was conducted to evaluate methods of enhancing the waterlogging resistance of soybean plant. Thus, we applied seven types of plant growth regulators (PGRs) to soybean plants and exposed them to waterlogged conditions for a total of 14 days. To evaluate stress resistance, we monitored plant growth characteristics data such as height, chlorophyll content, and chlorophyll fluorescence for 28 days after the initial waterlogging (14 days under waterlogging conditions and 14 days after waterlogging). According to the results, plant height was significantly increased by gibberellin A4 ($GA_4$) treatment compared to the control treatment and waterlogging-only treatment. However, we could not detect plant height owing to plant death when we applied abscisic acid (ABA). Except for $GA_4$ and ABA treatments, plant heights slightly decreased in all treatments compared to the waterlogging-only treatment. The chlorophyll content and chlorophyll fluorescence showed a similar tendency among PGR treatments. The chlorophyll content and chlorophyll fluorescence were significantly increased by ethephon and kinetin treatments 28 days after waterlogging compared to the waterlogging-only treatment. Consequently, kinetin and ethephon treatments induced more resistant phenotypes in soybean plants during or after exposure to waterlogging conditions.
This study was conducted to evaluate the quality and the quantity for manufacturing vinegar powder using spray drying. The $\beta$-cyclodextrin(CD) and gum arabic(GA) were used as well materials and the mixing ratio of CD and GA was ranged from 10:0 to 0:10. The moisture content of the vinegar powder of 2.5 of CD and 7.5 of GA was lowest among the other mixing ratios. At this proportion, the titratable acidity was highest as it had much included vinegar. The heat stability was not varied much with mixing ratio. However the stability of heat was maintained. Further the water absorption of powder was comparatively low. The manufactured powder vinegar shape was smooth round particles and stable structure by SEM and the particle size was small enough to form capsulation. In sensory evaluation, under these conditions the sourness was highest at 3.5. Therefore, the optimal mixing ratio at 2.5 of CD and 7.5 of GA in wall material was selected.
This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at $4{\pm}1.0^{\circ}C$. Seed size ranged $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, and weight of 1,000 seeds was $51.78{\pm}0.021mg$. Thus the seed was classified as 'dwarf seeds'. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at $20^{\circ}C$ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be $20^{\circ}C$ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in $GA_3$ and kinetin for 24 hours. Especially, 200~500 mg/L $GA_3$ treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as $GA_3$ was thought to be a useful method for raising seedling uniformly.
Journal of Korean Academy of Nursing Administration
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v.7
no.3
/
pp.473-485
/
2001
This study is intended to develop a reliable and appropriate instrument of the clinical nursing education. This research consisted of 4 steps. First step is construction of the content validity by 1 Korean literature professor and 1 teaching professor in Ga Chon Gil College, the pilot study for the content validity by 14 professors and survey with four points Likert Scale, which includes from the point 'strongly valid' to the point of 'strongly non-valid', by 113 head nurses who guide and evaluate the students in clinical practice. The third step is the test of validity and reliability of the preliminary evaluation tool. The fourth step is the test of validity and reliability of the developmental evaluation tool. The data were collected from Sep. 10th, 2001 to Sep. 28th, 2001. This study was analyzed by SPSS PC+ for descriptive statistics, factor analysis and Cronbach's Co-efficient Alpha of the collected data. The results of these analysis are like as follows. 1. Evaluation tool of Clinical practice consists of 16 items including four categories : factor 1 was labeled 'desirable attitude'(5 items), factor 2 was labeled 'correctly judgement and nursing problem solving'(4 items), factor 3 was labeled 'adaptive ability of nursing knowledge and skill'(4 items), factor 4 was labeled 'desirable human relationship'(3 items) and these contributed 71.992% of the variance in the total score. 2. Cronbach's Co-efficient Alpha for internal consistency was .9128 for the total 16 items. For further research, it need to develop a variable and reliable instrument of the student self-evaluation and instrument that based on community.
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