• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.219-224
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• 2013

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.5
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• pp.3610-3618
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• 2015

The congestion of railway vehicle and station shows up to 220%. Especially, transfer resistance of passenger increase rapidly by the collision of circulation. So increment of travel time, occurrence of safety accidents act as a factor that inhibits the utilization of urban railway station. In this paper, to improve traveling speed and comfort of urban rail passengers, urban transit passenger guidance system using congestion management model is proposed. The congestion management model that can mitigate a recurring/non-recurring congestion is constructed and the preliminary design of the system (middleware system, control system, guidance drive system) is carried out. Passenger Guidance System is configured by step for changing the external data into a form usable by the algorithm, step to perform the congestion management algorithm using the real-time data and historical data, step to control device based on the value that is calculated by congestion management algorithm, step to drive the device based on the information in the control system and circulation guidance devices. In the future, detail design will be performed based on the preliminary design. A prototype of the various devices according to the station structures and locations will be made. The control module of guidance device will be developed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.10
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• pp.6344-6353
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• 2014

According to the changes in society, the knowledge industrial center, which is a factory related to the manufacturing industry, is now including various programs, such as information and communication knowledge. This study defined the knowledge industrial center related law into three periods: deduct layout planning type and outside space composition elements by previous research; case analysis; and suggestions of the direction of layout planning and outside space plan. As a result of the analysis, first, before 1999, the layout of the building is made mainly with a car, but depending on the form of the site, it is configured as a centered, side layout. Second, in the case of distribution, the support facility on the lower part is integrated and the factory on the higher part is distributed, so it is partly integrated and the building is unified. Third, beginning with the activation of privately owned public space in a semi-industrial district according to architecture regulations in 1999, the outside space is wider but the construction area is reduced, so the outside space composition has been changing in a similar manner to the planning methods of business facilities, such as office buildings.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.3
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• pp.256-263
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• 1995

This paper presents the evidence on the considerably strong stratification - destratification(SD) phenomena during spring - neap tidal cycle in summer of 1992 based on the observed temperature, salinity and density data. To find out the main factors causing SD in the bay, we computed the rate of potential energy balance of the surface heat flux, tidal and wind stirring proposed by Simpson and Hunter (1974) and Simpson and Bowders (1981) using observed data. It was found that the energy of the wind stirring was one - order smaller than those of the heat flux and the tidal stirring. It means that the variation of stratification phenomena in the bay mainly depend on tidal stirring and sea surface heating in summer if there was no exceptionally strong wind event like a typhoon. Finally, we tested the effects of typhoon on the mixing characteristics of the bay using the example of a empirical typhoon model. It was found that when wind speed is larger than 15m/sec in Deukryang Bay, the wind energy was always larger than the average heating energy based on empirical typhoon model test. Particularly, typhoon passed on the left side of the bay, strong wind energy happened, which is almost the same as tidal energy of spring tide.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.1
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• pp.1-7
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• 2009

Most developed models are designed to determine pipe diameter, slope and overall layout in order to minimize the cost for the design rainfall for the optimal sewer layout. However, these models are not capable of considering the superposition effect of runoff hydrographs in the sewer pipes. The flow characteristics in the sewer pipes, such as the sewer layout, pipe diameter and slope, vary according to the design of the sewer system. In particular, when the sewer network is modified, the shapes of the runoff hydrographs in the sewer pipes also change because of the superposition effect. In this study, the sewer layout is designed to control and distribute the flows in the sewer pipes, while considering the runoff superposition effect, in order to reduce the inundation risk at each junction. This is accomplished by separating the inflows that enter into each junction by changing the way in which pipes are connected between junctions. And this model combines SWMM (Storm Water Management Model) to perform the hydraulic analysis for the flows in the sewer network. The current sewer layout was modified to minimize the peak outflow at outlet in Garak basin, Seoul, South Korea. As the results, the peak outflows at the outlet were decreased by approximately 20% for the design rainfall during 30 minutes and the total overflows were also decreased for the excessive rainfalls.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.3
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• pp.771-778
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• 2008

Effects of establishments of more sewer systems on water quality in Nam River, Deokchun River, and Jinyang Lake were analyzed for various scenarios using QUAL2E, WASP 7 water quality model. Three different scenarios were tested: 1) 20.8% of sewer diffusion rate which is same to the existing condition. 2) Expansion of sewer system to 65.2% which would emit less pollutants, BOD 2350.5 kg/d, TN 216.0 kg/d, TP 44.0kg/d. 3) Pollutants emission to maintain first grade water quality in Nam River, Jinyang Lake, BOD and TN in the case 2 were 7.69%, 2.10% lower than those in the case 1 in the Nam River. And in the Jinyang Lake, BOD, TN, and TP in the case 2 were 10.25%, 1.37%, 2.94% lower than those in the case 1. However the simulations showed that water quality could not hold down first grade water quality standard level with the establishments of more sewer systems. To satisfy the criteria in the Nam River and Jinyang Lake, BOD emission must be reduced 27.2%, 37.05% compared to those in the case 1.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.403-411
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• 2016

Recently, the number of occurrences of inundation and the severity of flood damage has increased rapidly as the frequency of localized heavy rainfall and the ratio of impervious area increased in urban areas. Most local governments focus on employing structural measures (e.g., the construction of detention reservoirs/pump stations, rehabilitation of drainage and sewer pipes) to prevent urban inundation. On the other hand, the effectiveness of implementing such structural measures is being dimished because there are already many inundation prevention facilities. The limitation of structural measures can be overcoming by employing non-structure measures, such as flood alerts and the operation of drainage facilities. This study suggests the pump operation rule (i.e., suggesting pump stop level) for a new detention reservoir operating method, which triggers the operation of a pump based on the water level at the monitoring node in urban drainage system. In the new reservoir operation, a total of 48 rainfall events are generated by the Huff distribution for determining the proper pump stop level. First, the generated rainfall events are distributed as frequencies, quartiles, and durations. The averaged system resilience value was determined to range from 1.2 m to 1.5 m is based on the rainfall-runoff simulation with rainfall generated by the Huff distribution. In this range, 1.2 m was identified considering the safety factor of 1.25 by the Standard on sewer facilities in 2011.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.111-117
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• 2017

This study examined the material properties of Korean rice husk ash (RHA) according to the manufacturing process, and evaluated the feasibility of its use as a new admixture for high strength concrete. For this purpose, its particle size distribution, chemical composition, and microstructure were analyzed under various parameters, such as calcination temperature ($400^{\circ}C$, $650^{\circ}C$, and $900^{\circ}C$) and the inclusion of a milling process. X-ray fluorescence analysis confirmed that the silicon oxide ($SiO_2$) content of RHA was improved to more than 92% with a calcination process at $650^{\circ}C$ or higher. In addition, microstructural analysis showed that the RHA calcined at $650^{\circ}C$ has a porous structure. Because of this, the absorption capacity of the RHA was improved. On the other hand, when the milling process was applied, the porous structure was destroyed; thus, the absorption capacity tended to decrease further. Based on the analysis results, it was concluded that RHA calcined at $650^{\circ}C$ can be used as an admixture for high strength concrete, which possesses functions of both a shrinkage reducing agent and a pozzolanic activator.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.463-470
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• 1998

To improve muscle quality and prolong freshness of sashimi, the effects of freezing-thawing condition on physicochemical and rheological properties of plaice muscle were investigated. Muscle tested were frozen with quick freezing (liquid nitrogen gas) or slow freezing ($-15^{\circ}C$ air). Transition time of zone of ice crystal formation was within 10 minute for quick freezing and 110 minute for slow freezing. Time required for thawing to $0^{\circ}C$ in muscle temperature by various thawing methods was shortest with $25^{\circ}C$ tap water, followed by $15^{\circ}C$ tap water, $10^{\circ}C$ tap water, $25^{\circ}C$ air, $5^{\circ}C$ tap water and $0^{\circ}C$ cold water. Breaking strength of muscle was higher in quickly frozen sample than in slowly frozen sample. According to sashimi term, changes in breaking strength of muscle did not show any difference in quickly frozen sample, while showed significant difference in slowly frozen sample. The remaining content of ATP was not effected by freezing speed, and ATP content was apt to higher in quickly thawed sample than in slowly thawed sample. IMP was the majority of ATP and it's related compounds of sample after freezing and thawing. Collagen matrix was weakened markedly in slowly frozen sample than in quickly frozen sample.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.523-528
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• 1998

To clarify the effect of life or death condition before cooling on the physicochemical properties of plaice, Paralichthys olivaceus muscle at the early period after death, the plaices were dipped in the refrigerated sea water ($0^{\circ}C$) either as alive or after anesthesia killing. These samples were stored at $0^{\circ}C$ sea water and the changes in rigor-mortis, ATP breakdown, content of ATP and its related compounds, breaking strength and lactate accumulation through storage were investigated. Acceleration of rigor-mortis, ATP breakdown and lactate accumulation were taster in the samples refrigerated as alive than in samples killed by anesthesia before cooling. ATP in samples refrigerated as alive showed little breakdown until 7.5 hrs but it was decomposed completely after 17.5 hrs storage. The breaking strength in muscle of plaice was 1736.2 $\pm$ 65.4 g immediately after killing. The breaking strength in samples dipped in refrigerated sea water as alive increased more rapidly and showed the maximum value over 7.5 hrs (2183.3$\pm$32.2 g), However, in case of samples killed by anesthesia before cooling, the value and time reached around the maximum breaking strength were 2126.3 $\pm$ 32.2 g and 12.5 hrs, respectively and then decreased until 30 hrs. From these results, it could be suggested that dipping in refrigerated sea water after anesthesia killing before cooling is more effective in maintaining freshness of fresh plaice muscle than refrigerating as alive.