• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.745-755
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• 2010

GTX(Great Train Express), the wide area express train of a big city center of metropolitan area, is the best new transportation method able to realize 'Low Carbon Green Growth' for solving traffic congestion of the metropolitan area with the connection the metropolitan area into 40-minute life zone by speeding up the standardized velocity, making the route straight and reducing the compensation of land using the space of 40~50m below the ground. In the transportation sector all over the world, if it turned into the public transportation system focusing on the train at a point of time that low carbon green revolution takes the initiative, national competitiveness would be enhanced by reducing the emission of carbon from cars by 1/6. Accordingly, this study is to look into the efficient operation in accordance with GTX construction superintended by currently Gyeonggi province and to consider the optimum operation plan of GTX that are proper for the actual circumstances of the country by comparatively analyzing overseas cases that took their positions as new transportation by curtailment of transit time through highspeed such as London area, Paris area, and Tokyo area etc that are similar to Korean metropolitan area.

• International Journal of Railway
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• 제3권2호
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• pp.54-59
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• 2010

In order to develop the deep-underground GTX (Great Train eXpress) in domestic, the running performance analysis of the propulsion system by a variety of route condition must be carried out before studying the specification and the development of the high-speed propulsion system with inverter and traction motor. Then it is necessary to study the running resistance properties of the high-speed traction system for the variety of tunnel type and vehicle organization method at first. In addition, the properties of the power requirement of the traction motors needed to maintain the balanced speed of the high-speed traction system are next studied. We need to study properties of the emergency braking distance caused by the highest operation speed of the high-speed traction system and present the fundamental design technologies to develop the high-speed traction system for the deep-underground GTX. Finally, the paper analyzes the applicability of the conventional Korean Tilting Train eXpress (TTX) propulsion system on the high-speed propulsion system for the deep-underground GTX.

• 대한의생명과학회지
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• 제23권3호
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• pp.238-250
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• 2017

Patient's primary tumor-derived tumor cell lines likely represent ideal tools for human tumor biology in vitro and in vivo. Here, we describe eight human gastric carcinoma cell lines derived from established tumors in vivo upon subcutaneous transplantation of primary gastric carcinoma specimens in BALB/c nude mice. These xenografted gastric tumor cell lines (GTX) displayed close similarity with primary gastric tumor tissues in their in vivo growth pattern and genomic alterations. GTX-085 cells were resistant to cisplatin, while GTX-087 was the most sensitive cell line. GTX-085 was the only cell line showing a metastatic potential. Epithelial cell adhesion molecule (EPCAM) expression was especially strong in all tissue samples, as well as in cell cultures. GTX-139, the largest tumor graft obtained after injection, displayed distinct expression of CD44v6, fibroblast growth factor receptor 2 (FGFR2), and prominin 1 (PROM1, also known as CD133). In summary, we established eight xenograft gastric cancer cell lines from gastric cancer patient tissues, with their histological and molecular features consistent with those of the primary tumors. The established GTX cell lines will enable future studies of their responses to various treatments for gastric cancer.

• 한국식품과학회지
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• 제40권1호
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• pp.8-14
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• 2008

본 연구는 야생꿀을 비롯한 벌꿀, 벌집채꿀 등 국내산 및 수입산 꿀의 안전관리를 위한 grayanotoxin(GTX)의 시험분석법 확립 및 실태조사를 위하여 수행하였다. GTX 표준품 I, II, III는 LC-MS/MS로 분자량을 확인한 후, 시판품인 III를 제외한 I과 II는 NMR을 이용하여 구조를 확인하였다. 총 111건(국내산 벌꿀25건, 국내산 야생꿀 21건, 벌집채꿀 13건, 수입산 벌꿀 44건, 수입산 야생꿀 8건)의 벌꿀시료는 메탄올을 사용하여 벌꿀-메탄올 용액을 만들어 tC18 cartridge에 loading 한 후, 여과된 액을 동량의 증류수로 희석하여, 이온화장치로 ESI를 장착한 triplequadrupole LC-MS/MS를 이용하여 분석하였다. LC의 용리액은 1% 포름산이 첨가된 "메탄올-물"을 사용하는 것이 10분 이내의 분석시간대에 나타나는 피크의 모양과 감도가 우수한 경향을 나타내었다. 본 방법을 이용하여 검체 중의 GTX I, II, III의 함유량을 조사한 결과 총 111건 중 수입산 야생꿀 3건(2.7%)에서 GTX I, II, III가 검출되었고, 수입산 야생꿀 1건에서 GTX I, III가 검출되었다. GTX I의 검출량은 최소 $3.13{\pm}0.00mg/kg$에서 최고 $12.93{\pm}0.01mg/kg$으로 나타났고 GTX II는 $0.84{\pm}0.01mg/kg$, $0.92{\pm}0.00mg/kg$, $1.08{\pm}0.01mg/kg$의 함량을 나타내어 GTX I 에 비해 낮은 수치를 나타내었다. GTX III는 최소 $0.25{\pm}0.01mg/kg$에서 최고 $3.29{\pm}0.74mg/kg$으로 함량에 큰 차이를 보였다. 본 방법을 이용한 총 111건의 벌꿀 시료의 GTX분석시 수입산 야생꿀 4건에서만 GTX가 검출됨을 알 수 있었다. 본 연구에서는 비 휘발성 또는 극성 때문에 GTX 분석시 GC 및 GC-MS에서 분석이 어려운 벌꿀시료를 대상으로 전처리 시간의 단축을 모색함과 동시에 LC-MS/MS를 이용한 시험분석법을 개발할 수 있었고, 모니터링을 통하여 네팔, 터키 등 특정 지역의 야생꿀의 섭취를 제한하는 과학적 근거를 마련할 수 있었다.

• 한국수산과학회지
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• 제33권6호
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• pp.550-553
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• 2000

해수에서 분리한 마비성패류독 분해 균주 Enterobactersp. CW-6 균주의 마비성 패류독소 최적 분해 온도 및 시험 균주의 독소 분해과정 중의 독소 함량 및 구성성분을 조사한 결과는 다음과 같다. 시험 균주는 배양 적온으로 확인된 $30, 35^{\circ}C$에서 배양 5일만에 각각 최초 첨가 독력의 $56.8, 44.1{\%}$, 12일 후에는 $93.0, 79.5{\%}$를 분해하였다. 그러나, 배양 적온에서 벗어날수록 균주의 독소 분해 활성은 감소하여 $20^{\circ}C$에서는 배양 12일 후에도 최초 독력의 $69.0{\%}$가 잔존하였다. 시험 균주 Enterobacter sp. CW-6는 $30^{\circ}C$에서의 조독소를 이용한 분해 활성 측정에서 초기 농도 38.2nmole/g의 마비성패류독을 배양 8일 및 12일째 각각 $88.4, 92.7{\%}$ 분해하였다. 정제 독소를 이용한 분해 시험에서, 조독소 분해과정 중에 일시적으로 증가하는 STX group은 GTX2, 3에서 전환된 것으로 확인되었다. 시험 균주 Enterobactersp. CW-6는 정제 독소에 대해서도 강한 분해 활성을 나타내었으며, 최초 농도 47 nmole/g 의 GTX1과 37 nmole/g GTX4를 분해 과정 12일 후에 각각 $100, 90.8{\%}$ 분해하였으며, GTX2, 3 혼합물에 대하여는 최초농도 25.6 nmole/g의 독소를 12일 후에 $66.4{\%}$까지 분해하였다.

• Journal of Electrical Engineering and Technology
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• 제7권2호
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• pp.200-206
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• 2012

In order to develop a deep-underground great train express (GTX) in South Korea, the specifications decision and development of a traction control system (including an inverter and a traction motor), which considers a variety of route conditions, must be advanced. In this study, we examined the running resistance properties of a high-speed traction system based on a variety of tunnel types and vehicle organization methods. Then, we studied the power requirements necessary for the traction motor to maintain balanced speed in the high-speed traction system. From this, we determined the design criteria for the development of a high-speed traction system for use in the deep-underground GTX. Finally, we designed a linear induction motor (LIM) for a propulsion system, and we used the finite element method (FEM) to analyze its performance as it travelled through deep-underground tunnels.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.159-164
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• 2013

The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay ($40{\mu}g/100g$ saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation ($r^2=0.9478$) was obtained for mussels than for oysters ($r^2=0.8244$). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.137-142
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• 2013

To understand critical aspects of paralytic shellfish poisoning (PSP) in a chief area of bivalve production in Korea, seasonal variation in PSP toxins in bivalves collected from Jinhae Bay, Korea in 2009 was surveyed by the pre-column high-performance liquid chromatography oxidation method. We also confirmed the profiles of major bivalves such as oysters Crassostrea gigas and mussels Mytilus galloprovincialis in Jinhae Bay. PSP toxins in the bivalves showed remarkable seasonal variation. PSP toxin levels were detected from April to May in 2009, and the highest total toxin levels at all stations were recorded in May. The major toxins in bivalves were gonyautoxin [GTX] 1&4 and C 1&2; in oysters GTX 2&3 were also detected as major components. GTX 1&4, which showed the highest PSP toxin levels at each station, accounted for the highest proportions of toxin components in mussels and oysters (64.5-71.3% and 41.4-42.4%, respectively). It was also confirmed that the highest toxicity (in ${\mu}g$ saxitoxin [STX] eq/g) was derived from GTX 1&4. The highest total toxicity (in ${\mu}g$ STX eq/g) was approximately 2-8-fold higher in mussels than in oysters collected from the same station. PSP toxin levels in bivalves differed significantly according to the sample collection station. However, the profiles of toxins in the bivalves did not show significant differences during the survey period according to sample collection station. This study shows that PSP toxin levels in some samples from Jinhae Bay were above the regulatory limit in Korea during a specific period in spring.

• 한국수산과학회지
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• 제39권1호
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• pp.49-54
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• 2006

Cultured cells of the toxic Alexandrium tamarense were fed to four mussel species, Mytilus coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus, to examine the interspecies and interlocality differences in the ability to accumulate paralytic shellfish poisoning (PSP) toxins. Toxin content of A. tamarense cells varied during culture period. In contrast, toxin composition in the cell (C1,2, GTX1-4 and neoSTX) was constantly stable. In feeding experiment, the four mussel species collected from Geoje intoxicated after uptake of A. tamarense. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) of M. coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus were $1,660{\pm}79,\;3,914{\pm}2,242,\;5,626{\pm}1,620\;and\;958{\pm}163$, respectively. Toxin profiles included C1,2, GTX1,4 and neoSTX as the major components, and dcGTX2,3, GTX2,3, neoSTX and STX as the minor ones. Toxin accumulation of three mussel species collected from Pohang, Geoje and Anmyon-do showed interspecies and interlocality differences. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) were $91{\pm}4,\;151{\pm}14,\;39{\pm}3$ in M coruscus, $189{\pm}1,\;231{\pm}11,\;206{\pm}15$ in M edu/is and $214{\pm}28,\;326{\pm}30,\;291{\pm}26$ in M. galloprovincialis in order of Anmyon-do, Geoje and Pohang.

• 화약ㆍ발파
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• 제39권3호
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• pp.24-34
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• 2021

정밀한 시차를 갖고 있는 전자뇌관은 다양한 건설현장에서 폭넓게 사용되고 있다. 전기뇌관이나 비전기뇌관을 이용한 발파현장에서 소음과 진동이 허용기준치를 초과하는 경우를 비롯하여, 발파작업에 의한 파쇄도를 개선하거나, 2차 소할의 비용을 절감하기 위해 사용된다. 또한 작업효율성을 극대화하여 공사기간을 단축시키며 비용을 절감하기 위해 전자뇌관을 이용하는 경우가 늘어나고 있는 추세이다. 본 사례는 도심지 내 GTX A 프로젝트에서 대단면 정거장 공사현장에 전자뇌관을 적용한 사례이다. 당 현장은 설계에 비전기뇌관을 이용하여 시공하도록 되어 있었으나, 발파작업을 진행하던 중 보안물건에 손상이 가해졌다. 이를 해결하기 위한 대안으로 전자뇌관을 이용한 발파공법을 검토하였다. 전자뇌관을 적용하여 발파소음과 진동에 대한 환경규제기준을 충족시켰으며 인근의 보안물건에 대한 손상을 방지할 수 있었다. 또한, 안전과 효율적인 시공을 위해 정거장 대단면을 1회 동시발파를 실시하였으며, 이를 통해 계획대비 공사기간을 단축시켰다.