• Korean Journal of Food Science and Technology
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• v.37 no.5
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• pp.850-855
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• 2005

Model was developed to predict the growth of Listeria monocytogenes in raw pork. Experiment condition for model development was full 5-by-7 factorial arrangements of temperature (0, 5, 10, 15, and $20^{\circ}C$) and time (0, 1, 2, 3, 18, 48, and 120 hr). Gompertz values A, C, B, and M, and growth kinetics, exponential growth rate (EGR), generation time (GT), lag phase duration (LPD), and maximum population density (MPD) were calculated based on growth increased data. GT and LPD values gradually decreased, whereas EGR value gradually increased with increasing temperature. Response surface analysis (RSA) was carried out using Gompertz B and M values, to formulate equation with temperature being main control factor. This equation was applied to Gompertz equation. Experimental and predictive values for GT, LPD, and EGR, compared using the model, showed no significant differences (p<0.01). Proposed model could be used to predict growth of microorganisms for exposure assessment of MRA, thereby allowing more informed decision-making on potential regulatory actions of microorganisms in raw pork.

• Proceedings of the Korean Environmental Health Society Conference
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• 2004.06a
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• pp.178-181
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• 2004

Di-n-butyl phthalate (DBP) is used extensively in the plastic industry and has been known as an environmental hormone (endocrine disruptor). Present study was undertaken to examine whether DBP can induce oxidative stress in mice. In this study, oxidative stress was measured in terms of the modification of lipid peroxidation and gamma-glutamyltranspeptidase (${\gamma}-GT$) activity. The activity of ${\gamma}-GT$, the level of lipid peroxidation and serum toxicity index were measured in male ICR mice after treatment with DBP (5 g/kg, po). Administration of DBP was found to significantly increase the level of lipid peroxidation approximately 2 fold in liver. The activity of ${\gamma}-GT$ in the liver of DBP-exposed animals was also increased approximately 2.5 fold. However, DBP did not alter the parameters for hepatotoxicity and nephrotoxicity such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine. These results indicate that DBP can induce oxidative stress in mice. The ${\gamma}-GT$ activity is considered to be increased as one of the adaptive defense mechanisms to oxidative stress induced by DBP.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1057-1060
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• 2004

In this study, the wear characteristics of five different dental composite resins cured by conventional halogen light and LED light sources were investigated. Five different dental composite resins of Surefil, Z100, Dyract AP, Fuji II LC and Compoglass were worn against a zirconia ceramic ball using a pin-on-disk type wear tester with 15 N contact force in a reciprocal sliding motion with sliding distance of 10 mm/cycle at 1Hz under the room temperature dry condition. The wear variations of dental composite resins were linearly increased as the number of cycles increased. It was observed that the wear resistances of these specimens were in the order of Dyract AP ＆gt; Surefil ＆gt; Compoglass ＆gt; Z100 ＆gt; Fuji II LC. On the morphological observations by SEM, the large crack formation on the sliding track of Fuji II LC specimen was the greatest among all resin composites. Dyract AP showed the least wear with few surface damage. There is no significant difference in wear performance between conventional halogen light curing and light emitting diodes curing sources. It indicates that a light emitting diodes (LED) source can replace a halogen light source as a curing unit for composite resin restorations.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.539-542
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• 2007

Glycosyltransferase family 1 (UOT) uses small chemicals including phenolics, antibiotics, and alkaloids as substrates to have an influence in biological activities. A glycosyltransferase (XcGT-2) from Xanthomonas campestris was cloned and consisted of a 1,257 bp open reading frame encoding a 45.5 kDa protein. In order to use this for the modification of phenolic compounds, XcGT-2 was expressed in Escherichia coli as a glutathione S-transferase fusion protein. With the E. coli transformant expressing XcGT-2, biotransformation of flavonoids was carried out. Flavonoids having a double bond between carbons 2 and 3, and hydroxyl groups at both C-3' and C-4', were glycosylated and the glycosylation position was determined to be at the hydroxyl group of C-3', using nuclear magnetic resonance spectroscopy. These results showed that XcGT-2 regiospecifically transferred a glucose molecule to the 3'-hydroxyl group of flavonoids containing both 3' and 4'-hydroxyl groups.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.154-160
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• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.

• Bulletin of the Korean Chemical Society
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• v.26 no.2
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• pp.260-264
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• 2005

The nature of the active site of Tobacco acetohydroxyacid synthase (AHAS) in the substrate- and cofactorbinding was studied by kinetics and fluorescence spectroscopy. The substrate saturation curve does not follow Michaelis-Menten kinetics at different temperatures (7, 21 and 37 ${^{\circ}C}$), pH (6.5, 7.5 and 8.5) and buffers (Tris-HCl and MOPS). The concentration of one half of the maximum velocity ($S_{0.5}$) decreased in the following order: pyruvate $\gt$ ThDP $\approx$$Mg^{+2}$ $\gt$ FAD. However, the catalytic efficiency (K$_{cat}/S_{0.5}$) inversely decreased in the following order; FAD $\gt$ $Mg^{+2}$ $\approx$ThDP $\gt$ pyruvate, indicating that the cofactors by in decreasing order; FAD, $Mg^{+2}$, ThDP, affect the catalysis of AHAS. The dissociation constant ($K_d$) of the intrinsic tryptophan fluorescence decreased with the same tendency of the concentration of one half of the maximum velocity ($S_{0.5}$) decreasing order. This data provides evidence that the substrate and cofactor binding natures of the active site, as well as its activation characteristics, resemble those of other ThDP-dependent enzymes.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.28 no.7
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• pp.41-47
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• 2014

The on-line partial discharge (PD) in stator windings of air-cooled gas turbine (GT) generator (119.2MVA, 13.8kV) is measured and analyzed in this paper. This generator was designed by global vacuum pressure impregnation (VPI). The generator failed two times at top bar (16T) of phase B in the stator slot. Six epoxy-mica capacitors were installed in three phases of GT generator. On-line PD test was performed on GT generator using turbine generator analyzer (TGA). TGA showed that the normalized quantity number (NQN) and the PD magnitude($Q_m$) were high in phase B. Internal discharges were generated in phases A, B and C. The trend analysis of NQN and $Q_m$ value are obtained in order to monitor the insulation condition in GT generator stator windings. Phases A and C were in good condition. But phase B had deteriorated significantly

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.888-893
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• 2021

The International Atomic Energy Agency has developed a tomographic imaging system for accomplishing the total fuel rod-by-rod verification time of fuel assemblies within the order of 1-2 h, however, there are still limitations for some fuel types. The aim of this study is to develop a deep learning-based denoising process resulting in increasing the tomographic image acquisition speed of fuel assembly compared to the conventional techniques. Convolutional AutoEncoder (CAE) was employed for denoising the low-quality images reconstructed by filtered back-projection (FBP) algorithm. The image data set was constructed by the Monte Carlo method with the FBP and ground truth (GT) images for 511 patterns of missing fuel rods. The de-noising performance of the CAE model was evaluated by comparing the pixel-by-pixel subtracted images between the GT and FBP images and the GT and CAE images; the average differences of the pixel values for the sample image 1, 2, and 3 were 7.7%, 28.0% and 44.7% for the FBP images, and 0.5%, 1.4% and 1.9% for the predicted image, respectively. Even for the FBP images not discriminable the source patterns, the CAE model could successfully estimate the patterns similarly with the GT image.

• Smart Structures and Systems
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• v.31 no.2
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• pp.199-212
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• 2023

Nowadays fuzzy logic in control applications is a well-recognized alternative, and this is thanks to its inherent advantages. Generalized type-2 fuzzy sets allow for a third dimension to capture higher order uncertainty and therefore offer a very powerful model for uncertainty handling in real world applications. With the recent advances that allowed the performance of general type-2 fuzzy logic controllers to increase, it is now expected to see the widespread of type-2 fuzzy logic controllers to many challenging applications in particular in problems of structural control, that is the case study in this paper. It should be highlighted that this is the first application of general type-2 fuzzy approach in civil structures. In the following, general type-2 fuzzy logic controller (GT2FLC) will be used for active control of a 9-story nonlinear benchmark building. The design of type-1 and interval type-2 fuzzy logic controllers is also considered for the purpose of comparison with the GT2FLC. The performance of the controller is validated through the computer simulation on MATLAB. It is demonstrated that extra design degrees of freedom achieved by GT2FLC, allow a greater potential to better model and handle the uncertainties involved in the nature of earthquakes and control systems. GT2FLC outperforms successfully a control system that uses T1 and IT2 FLCs.

• Proceedings of the Korea Information Processing Society Conference
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• 2008.11a
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• pp.1215-1218
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• 2008

IEEE 802.15.4 기반의 무선 센서 네트워크는 다양한 종류의 센서들로 구성된다. 이러한 센서들은 각각 다양한 주기들로 중심 노드에게 데이터를 전송하고자 한다. 슈퍼프레임마다의 주기적인 전송을 위해 IEEE 802.15.4에서는 GTS(Guaranteed Time Slot) 메커니즘을 제공한다. 하지만 하나의 센서 네트워크에서 슈퍼프레임 길이는 모두 동일하기 때문에 이보다 긴 주기를 갖는 센서들은 GTS를 할당받고도 데이터를 전송하지 않는 경우가 발생할 수 있다. 때문에 IEEE 802.15.4의 GTS 메커니즘에서는 낮은 사용 효율의 문제가 발생한다. 본 논문은 이러한 문제를 보완하고자 주기적 GTS 할당 방법(PGTS: Periodic Guaranteed Time Slot)을 제안한다. 주기적 GTS 할당 방법에서는 단말들이 GTS 요청 시 자신의 데이터 전송 주기를 PAN 코디네이터에게 보고하고, PAN 코디네이터는 이를 고려하여 단말의 주기에 따른 슈퍼프레임에서만 GTS 구간을 할당한다. 이를 통해 주기적 GTS 할당 방법은 기존 GTS 할당 방법보다 대역의 낭비를 줄여 GTS를 효율적으로 사용가능하도록 하고 더 많은 수의 단말들에게 GTS를 배정할 수 있게 한다. 본 논문에서는 시뮬레이션 결과를 통해 주기적 GTS 할당 방법이 IEEE 802.15.4의 GTS 할당 방법보다 효율적이며 더 많은 수의 단말에게 GTS를 할당할 수 있음을 확인하였다.