• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.689-698
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• 2016

Specific metabolic network responses to mineral starvation are not well-defined. We examined a detailed broad-scale identification of metabolic responses of tomato leaf and root to N, P or K starvation. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and starved (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Overall, the levels of metabolites (lipids, nucleotides, peptides and secondary metabolites) presented in this paper largely showed mineral- and tissue-specific responses. Most strikingly, G3P (glycerol-3-P), GPC (glycerol-P-choline) and choline phosphate responded differently to a type of mineral; an increase in N or K starvation and a decrease in P starvation. A dramatic increase in the levels of secondary metabolites, in particular, rutin and chlorogenate in both tomato tissues during N starvation were observed. Based on these data, it is necessary to clearly elucidate an unknown event taking place in a variety of abiotic impacts, and we are now studying to expand our knowledge on metabolic- and proteomic-responses using GS-MS and LC-MS.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.171-176
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• 2003

The complete nucleotide sequences of the genomic RNAs of Soybean mosaic virus strains GS (SMV-G5) and G7H (SMV-G7H) were determined and compared with sequences of other SMV strains. Each viral RNA was determined to be 9588 nucleotides in length excluding the poly (A) tail and contained an open reading frame to encode a polyprotein subsequently processed into up to ten proteins by proteolytic cleavage. Com-parison of the amino acid sequences with those of other SMV strains showed high percentage of amino acid sequence homology with the same genome organization. The nucleotide and the deduced amino acid sequences between SMV-G5 and SMV-G7H were greater than 99% identity. When compared with those of other SMV strains in a phylogenetic analysis of the nucleotide and deduced amino acid sequences, they formed a distinct virus clade showing over 97％ amino acid identity, but were more distantly related to the other potyvirus (44.1-69.6% identity). Interestingly, SMV G7H strain caused a severe mosaic or necrosis symptom in soybean cultivars including Jinpum-1, Jinpum-2, and Sodam, whereas, no symptom was observed in SMV-G5 inoculation. Complete nucleotide sequences of these strains will give clues for determining symptom determinant(s) in future research.

• Reproductive and Developmental Biology
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• v.31 no.2
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• pp.83-90
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• 2007

Insulin-like growth factor II (IGF2) and H19 genes are mutually imprinted genes which may be responsible for abnormalities in the cloned fetuses and offspring. This study was performed to identify putative differentially methylated regions (DMRs) of porcine H19 locus and to explore its genomic imprinting in in vitro fertilized (IVF) and somatic cell nuclear transferred (SCNT) embryos. Based on mice genomic data, we identified DMRs on H19 and found porcine H19 DMRs that included three CTCF binding sites. Methylation patterns in IVF and SCNT embryos at the 2-, 4-, $8{\sim}16$-cells and blastocyst stages were analyzed by BS (Bisulfite Sequencing)-PCR. The CpGs in CTCF1 was significantly unmethylated in the 2-cell stage IVF embryos. However, the 4- (29.1%) and $8{\sim}16$-cell (68.2%) and blastocyst (48.2%) stages showed higher methylation levels (p<0.01). On the other hand, SCNT embryos were unmethylayted ($0{\sim}2%$) at all stages of development. The CpGs in CTCF2 showed almost unmethylation levels at the 2-,4- and $8{\sim}16$-cell and blastocyst stages of development in both IVF ($0{\sim}14.1%$) and SCNT ($0{\sim}6.4%$) embryos. At all stages of development, CTCF3 was unmethylated in IVF ($0{\sim}17.3%$) and SCNT ($0{\sim}1.2%$) embryos except at the blastocyst stage (54.5%) of IVF embryos. In conclusion, porcine SCNT embryos showed an aberrant methylation pattern comprised to IVF embryos. Therefore, we suggest that the aberrant methylation pattern of H19 loci may be a reason for increased abnormal fetus after embryo transfer of porcine SCNT embryos.

• Journal of Dental Rehabilitation and Applied Science
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• v.25 no.4
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• pp.417-429
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• 2009

Since the introduction of the concept of osseointegration in dental implants, high long-term success rates have been achieved and accepted as viable option for the treatment of fully and partially edentulous patients. Although the use of domestic implants have increased dramatically, there are few studies on domestic implants with clinical and objective long-term data. 96 endosseous implants placed in 31 patients at Wonkwang University Sanbon Dental Hospital were examined to determine the effect of various factors on implant survival rate and marginal bone loss, through clinical and radiographic results. The design of endosseous implant used to this study is straight with the microthread.(GS II RBM Fixture) 1. 3 fixtures were lost, resulting in 96.9% cumulative survival rate. 2. Survival rate in fifties was significantly lower (93.6%) and no significant difference in marginal bone loss was found according to gender. 3. Survival rates were 95.6% in the maxillary molar area and 97.3% in the mandible molar area. 4. No significant difference in survival rate was found according to presence of bone grafts, type of prostheses, implant position, and length and diameter of implant. 5. A factor influencing marginal bone loss was presence of type of prostheses, while facters such a length, diameter of fixture and bone grafts had no statistically significant effect on crestal bone loss. This study indicates the amount of marginal bone loss around implant has maintained a relative stable during follow-up periods.

• Journal of Environmental Science International
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• v.1 no.1
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• pp.51-59
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• 1992

Two comparative poplar clones (I-214: Populus euramerinm, Peace: P koreana x p. trihocarpa) were exposed to two $CO_2$ concentrations (350 or 2, 000 ${\mu}L L^{-1} CO_2$) for 21 days. When both poplar clones were compared at growth conditions, the net photosynthetic rate ($P_N$) in $CO_2$-enriched ($2, 000{\mu}L L^{-1} CO_2 = C_{2, 000}$) plants become about 50-60% higher than that of 350 ${\mu}L L^{-1} CO_2 (=C_{350}$ Plants on 7 days treatment. But the enhancement of PN by high $CO_2$ was not maintained throughout all the experimental period. At 21 days, there was no difference of photosynthetic rates between $C_{350}$ and $C_{2000}$ plants. In contrast with photosynthesis, the response of leaf conductance to the elevated $CO_2$ concentration was very different between I-214 and Peace. During all experimental period, leaf conductance ($g_{s}$) of $C_{2000}$ plants is 50% lower than that of the $C_{350}$ plants for I-214, while there is no difference of gs between the plants of $C_{350}$ and $C_{2, 000}$ for Peace. The results of gs in Peace indicate that decreased photosynthetic rate after 21 days in $C_{2, 000}$ Plants for two poplar clones is possibly due to non-stomatal factors. To investigate the non-stomatal factors, starch accumulation and ribulose-1, 6-bisphosphate carboxylase (RuBPCase) were measured. We found significant accumulation of starch in two poplar clones exposed to high $CO_2$, especially starch of I-214 in $C_{2, 000}$ become 3.5 times higher than in $C_{350}$ plants at 21 days. This suggests that high proportion of photosynthates was directed into starch. After 21 days, the activity of ribulose-1, 6-bisphosphate carboxylase of $C_{2, 000}$ plants become decreased in 40-50% compared with that of the $C_{350}$ plants. Two poplar clones show the same trend to RuBPCase declines under high $CO_2$ concentration, although the decline is more significant for I-214. The results reported here suggest that starch accumulation and decreased RuBPCase activity in $C_{2, 000}$ plants can be partly ascribed to the loss of photosynthetic efficiency of high $CO_2$-grown poplar plants.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.227-238
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• 2004

Rhododendron micranthum is an endangered species in Korea. In order to develop the strategy of gene diversity conservation, estimation of the amount of genetic diversity, the genetic variation and relationship in the native populations of Rh. micranthum was performed on the basis of AFLP and RAPD analysis. Analysis of 56 accessions derived from 6 populations of Rh. micranthum with four AFLP primer combinations and ten RAPD primers detected a total of 33 polymorphic AFLP fragments and 15 polymorphic RAPD fragments, respectively. By UPGMA cluster analysis with molecular markers, the 56 accessions were grouped into three major clusters at 73.3% genetic similarity; group I consists of most accessions of populations I, II, IV, V and Ⅵ, group II consists of 7 accessions of population III, and group III consists of only two accessions of population IV. The geographic locations of the most accessions derived from six populations were not related to their position in the UPGMA cluster analysis, except for several accessions of populations III and IV. The genetic similarity of among six populations measured by AFLP and RAPD markers ranged from 0.66 to 0.99. Among them, population Ⅵ showed the highest GS with means of 0.87, while population I showed the lowest GS with means of 0.78. This result will be useful for designing the strategy of conservation in the native populations of Rh. micranthum.

• Journal of the Korean Geotechnical Society
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• v.26 no.8
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• pp.77-88
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• 2010

In this study, 1 g shaking table group pile tests were performed for various conditions of subgrade and pile spacing. The pile spacing was changed from three to seven times of pile diameters. It could be confirmed that the dynamic p-y curves for the group pile observed as the results of a series of shaking table tests show difference according to the pile spacing, the pile location within the pile group, the relative density of subgrade and the excess pore pressure during earthquake. The dynamic p-multipliers were calculated by comparing the dynamic p-y backbone curves of a single pile suggested by Yang (2009) and dynamic p-y curves for the group pile. Dynamic p-multiplier values overall increase as the relative density of subgrade and amplitude of input acceleration increase. The dynamic group pile effect was neglected, if the pile spacing was seven times as large as pile diameters. It was found that the exisiting p-multiplier values suggested by various researchers for the static and dynamic loading, and the values recommended by globally used specifications show difference with the test results by up to 0.7 (approximately 70%). Therefore, the dynamic p-multipliers were newly suggested according to the pile spacing and the relative density of subgrade using the test results.

• Journal of the Korean Geotechnical Society
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• v.26 no.2
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• pp.45-54
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• 2010

The SCW numerical model described in the companion paper was used to carry out a comprehensive parametric study to evaluate the performance of the SCW. The five ground related parameters, which are porosity, hydraulic conductivity, thermal conductivity, specific heat, geothermal gradient, and five SCW design parameters, which are pumping rate, well depth, well diameter, dip tube diameter, bleeding rate, were used in the study. Two types of numerical simulations were performed. The first type was used to perform short-term (24-hour) simulation, while the second type 14 day simulation. The study results indicate that the parameters that have important influence on the performance of SCW were hydraulic conductivity, thermal conductivity, geothermal gradient, pumping rate, and bleeding rate. The thermal conductivity had the most important influence on the performance of the SCW. With the increase in the geothermal gradient, the performance increased in the heat mode, but decreased in the cooling mode. The hydraulic conductivity influenced the performance when the value was larger than $10^{-4}m/s$. The depth of the well increased the performance, but at the cost of increased cost of boring. The bleeding had an important influence on SCW, greatly enhancing the performance at a limited increased cost of operation. Overall, this study showed that various factors had a cumulative influence on the performance of the SCW, and a numerical simulation can be used to accurately predict the performance of the SCW.

• Korean journal of applied entomology
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• v.63 no.1
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• pp.53-61
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• 2024

The susceptibility of Aphis gossypii populations collected from three fields (WJ, CC, and GS) was evaluated to three insecticides (afidopyropen, chlorfenapyr and cyantraniliprole) and three binary mixtures. Three field populations showed resistance ratios of over 100 to all insecticides. The Combination Index (CI), %M_(synergism), Co-Toxicity Coefficient (CTC), Wadley Ratio (WR), Synergism Ratio (SR) and Abbott Ratio (AR) were used to evaluate combined effect of the insecticides. Afidopyropen + chlorfenapyr (CI ≤ 0.16; %M_(synergism) ≥ 94; CTC ≥ 764.5; WR ≥ 6.4; SR ≥ 6.9 and AR ≥ 1.1) showed a synergism in all filed populations. WJ and CC populations showed a synergism in all binary mixtures of insecticides, but GS population showed an antagonism for chlorfenapyr + cyantraniliprole (CI, 1.63; %M_(synergism), 30; CTC, 64.0; WR, 0.6 and AR, 0.54) and afidopyropen + cyantraniliprole (CI, 6.7; %M_(synergism), 1; CTC, 19.8; WR, 0.2 and AR ≤ 0.55). All mixtures (afidopyropen + chlorfenapyr, chlorfenapyr + cyantraniliprole and afidopyropen + cyantraniliprole) showed a control value of over 99% after 21 days of treatment in the field. This study highlights that binary mixtures of three insecticides serve as an effective control strategy for A. gossypii.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.138.1-138.1
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• 2010

정부는 그린홈 100만호 보급 사업에서 연료전지의 보급 목표를 2010년 200대 2011년 300대, 2012년 500대로 결정하였고 이에 따라 2012년까지 누적 보급대수가 1000대에 달할 전망이다. 본 연구에서는 현재 상업화 되어있는 국내업체의 1kW급 가정용 연료전지 시스템을 2009년에 도입되는 것으로 가정하여 경제성평가를 수행하였다. 분석의 편의상 2009년 서울 지역 난방면적 $100m^2$을 기준으로 전제하였고, 대표가정의 전기와 열수요는 CES 소형 열병합 사업 타당성 분석 프로그램(GS파워, 2006)을 활용하여 구했다. 비용의 경우 기존 보일러의 설비가격은 60만원이며 연료전지시스템의 설비가격은 1200만원이다. 다만 연료전지의 고가 소모품인 스택은 2007년 발간된 한국에너지 기술연구원의 신재생에너지 경제성평가 보고서를 인용하여 스택의 수명은 5년, 교체비용은 1000만 원이나 5년마다 30%의 비용 하락을 전제하였다. 또한, 연료전지시스템의 수명을 20년으로 가정하였으며 할인율은 5.5%를 가정하였다. 한편, 가정용 연료전지의 최적 운전방안을 찾기 위해서 기존 설비를 이용한 비용과 전기추종운전, 열추종운전의 시뮬레이션을 수행한 뒤 세가지 결과를 시간대별로 비교함으로써 최적의 시간대별 운전방식을 선택하는 복합추종운전의 비용을 분석하였다. 시뮬레이션결과, 기존 설비 이용 시 에너지 비용은 1,934,864원으로 분석된 반면 연료전지를 이용한 전기추종은 1,123,691원, 열추종은 1,180,425원, 복합추종은 1,121,174원으로 계산되었다. 한편 편익면에서는 복합추종운전시 813,690원의 편익이 발생하는 것으로 분석되었으며 B/C ratio의 결과는 0.405로 현재로서는 연료전지 시스템이 경제성이 없는 것으로 분석되었다. 따라서 정부는 연료전지의 보급목표와 민간 주도의 자생적인 시장형성을 촉진하기 위해서 단순 설치 보조금 이외에 연료전지시스템과 스택의 비용을 획기적으로 저감 시킬 수 있는 기술개발을 촉진하는 정책 병행이 필요해 보인다.