• Title/Summary/Keyword: GR 24

검색결과 149건 처리시간 0.028초

Modified Drum Priming and Exogenous Application of 24-Epibrassinolide (24-EBL) for Enhancing Germination under High Temperature Condition in Lettuce Seeds

  • Kang, Won Sik;Kim, Min Geun;Kim, Du Hyun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 추계학술대회
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    • pp.86-86
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    • 2018
  • This study was conducted to investigate the effects of modified drum priming and 24-Epibrassinolide (24-EBL) treatment to improve the seed quality for export. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 16 and 24 h in a container with a relative humidity of 99% at 26 rpm for a modified drum priming treatment. The treated seeds were sown at $20^{\circ}C$ and $30^{\circ}C$ (12/12h, light/dark) with four replications of 25 seeds on pleated paper. The seeds were hydrated with water or 24-EBL solutions of $10^{-7}$, $10^{-8}$ and $10^{-9}M$, respectively. The germination of the modified drum primed seeds (24 h incubation after 60% SMC hydration) improved to 1.6 days mean germination time (MGT) and $46%{\cdot}day^{-1}$ germination rate (GR), while the untreated seeds showed 2.1 days MGT and $28%{\cdot}day^{-1}$ GR. The modified drum priming (60% SMC and 24 h incubation with $10^{-9}M$ 24-EBL) showed improved results in MGT (1.8 days) and GR (55%) at $20^{\circ}C$, whereas untreated seeds showed 2.3 days MGT and 44% GR. Under $30^{\circ}C$, germination of modified drum primed seeds was significantly improved in GP (80%), GR ($31%{\cdot}day^{-1}$), HS (55%) and MGT (3.3 days), however, untreated seeds showed decreased GP (27%), GR ($22%{\cdot}day^{-1}$), HS (55%) and MGT (4.8 days). This study showed that the germination of lettuce seeds is enhanced by 24 h drum incubation with 24-EBL and this method can be used effectively to achieve the benefits of early germination and uniform seedling development. In addition, these treatments circumvent thermo-dormancy of lettuce seed and have a possibility of high-quality and environment-friendly seed processing.

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冠岳山에 있어서 空中에 飛散하는 花紛層에 關한 硏究 (A Study on the Airborne Pollen Calender in Mt. Kwanak)

  • Chang, Nam-Kee;Nam-il Kim
    • The Korean Journal of Ecology
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    • 제8권1호
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    • pp.39-48
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    • 1985
  • The airborne pollens were investigated by Durhan's pollen trap in Mt. Kwanak from March 1, 1984 to February 28, 1985. The observed airborn pollens were identified 22 families and 49 genera, which were divided into 27 tree pollens. Most airborne pollens were found from late-March to late-September and a few from early-November to late-February, 1985. The first observed pollen of the year was Alnus on March 5 and the last was Artemisia on Decomber 19, 1984. The maximum amount of daily airborne pollens was 823 grains/cm2 on May 16. Peak day and the maximum amount of major airborne pollens were as follows; 166 grains/cm2/day of Alnus pollen on April 4, 38 gr. of Populus on April. 13, 22 gr. of Quercus on May 10, 791 gr. of Pinus on May 26, 139 gr. on May 26 and 104 gr. of Graminae on September 6, 24 gr. of Ambrosia on Sep. 10 and 236 gr. on May 26 and 104 gr. of Graminae on September 6, 24 gr. of Ambrosia airborne pollen for one year was 12,733 grains/cm2 which were composed of 61% tree pollens and 39% herb ones. Decreasing order of pollen counts was Pinus, Alnus, Quercus and Populus in trees, and Graiminae, Artemisia and Ambrosia in herbs. Most of the airborn pollens were deposited in the day-time and a few at night, the maximum at 2∼4p.m.and the mininum at 8∼10 p.m.

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��프신의 고정화(固定化)에 관한 연구(硏究) (A Study on Immoblization of Pepsin)

  • 박종래
    • Current Research on Agriculture and Life Sciences
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    • 제3권
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    • pp.166-173
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    • 1985
  • ��신을 DEAE-cellulose, CM-cellulose, Sepharose-4B와 porous glass beads을 사용(使用)하여 고정화(固定化)시켰던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. CM cellulose와 DEAE-cellulose의 ��신 흡착량(吸着量)은 건조된 bead 1 gr에 대하여 각각 10 mg와 15 mg이었고 glutaralde을 사용(使用)함으로서 20 mg와 27 mg로 흡착량(吸着量)이 증가(增加)하였고 효소(酵素)의 활력(活力)은 용해성효소(溶解性酵素)에 비해 각각 22%, 24%로 나타났다. 2. Sepharose-4B을 cyanogen bromide을 사용(使用)하여 활성화(活性化)시킨후 glutaraldehyde을 사용(使用)하여 공유결합을 유도하였던 바 건조된 bead 1 gr에 ��신 19 mg가 통합(統合)되었고 효소(酵素)의 활력(活力)은 23%로 나타났다. 3. Porous glass beads의 표면(表面)을 succineamido - propyl glass beads의 형태(形態)로 유도체를 만든후 ��신을 고정화(固定化)시켰을 때 bead 1 gr가 ��신 40 mg을 통합(統合)할 수 있었고 효소(酵素)의 활력(活力)은 용해성효소(溶解性酵素)에 비해 45%로 나타났다.

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인삼이 사람태아골모세포의 세포주기조절에 미치는 영향 (Effects of Ginseng Radix on the Cell Cycle Regulation in Human Fetal Osteoblast)

  • 김대겸;이용배;박상기;유형근;유경태;김윤철;신형식
    • Journal of Periodontal and Implant Science
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    • 제33권3호
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    • pp.415-437
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    • 2003
  • Ginseng Radix(GR) had been used widely from oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The results were as follows. Increased cell proliferation was observed in cells exposed to 100 ng/ml, 10 ng/ml of GR-1 at 12 hours and 24 hours, 1 ${\mu}g$/ml of GR-1 at 48 hours, and 100 ${\mu}g$/ml, 10 ${\mu}g$/ml of GK-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2. CDK 4 and CDK 6 were increased in the group of treated with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3. On the other hand, p21 was decreased in the treatment group with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, 10 ${\mu}g$/ml of GR-3, and p53 and p16 was decreased in the treatment group with 100 ng/ml of GR-1, 100 ${\mu}g$/ml and GR-3 and pRb was decreased in the all treatment groups except 1 ${\mu}g$/ml of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1 , Cyclin E, CDK 2, CDK 4, CDK 6 and decreased cell cycle regulation protein levels of p21, pRb.

중력 장-흐름 분획법을 이용한 전분 입자의 swelling에 관한 연구 (Study on swelling of starch granules using gravitational field-flow fractionation (GrFFF))

  • 김선태;서소연;이승호
    • 분석과학
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    • 제24권4호
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    • pp.249-255
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    • 2011
  • 물의 흡수에 의한 전분알갱이의 swelling은 물과 친화력이 있는 새로운 부분의 노출에 의하여 구조적인 변화로써 점진적으로, 때로는 갑작스럽게 변화를 일으킨다. 이러한 이유로 인해서, 물과의 접촉 시간에 따르는 전분 알갱이의 크기 또는 모양의 변화에 관심을 가진다. 중력 장-흐름 분획법(gravitation field-flow fractionation, GrFFF)은 마이크론 범위의 크기를 가지는 입자들을 분리하는 데에 유용한 분리기술이다. 본 연구에서는 감자와 고구마 전분알갱이들의 크기와 크기 분포도를 결정하는 데에 있어서 GrFFF의 응용가능성을 검사하고자 한다. GrFFF를 이용하여 물과의 접촉 시간에 따르는 전분 알갱이들의 크기분포도 변화를 모니터하였다. GrFFF로부터 얻어진 전분알갱이들의 크기 및 크기분포는 광학현미경(optical microscopy, OM) 결과와 비교하였다. 결과적으로 감자와 고구마 전분의 swelling은 물과의 접촉시간이 증가함에 따라 전분알갱이의 크기가 증가하며, 전분의 종류가 달라지면 swelling 속도가 달라짐을 확인하였다.

Determination of Optimal Harvest Time of Chuchung Variety Green Rice® (Oryza sativa L.) with High Contents of GABA, γ-Oryzanol, and α-Tocopherol

  • Kim, Hoon;Kim, Oui-Woung;Ha, Ae Wha;Park, Soojin
    • Preventive Nutrition and Food Science
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    • 제21권2호
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    • pp.97-103
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    • 2016
  • In our previous study, an early-maturing variety of rice (Oryza sativa L.), Jinbu can have feature with unique green color, various phytochemicals as well as nutritive components by the optimal early harvesting, called Green Rice$^{(R)}$ (GR). The aims of the present field experiments were to evaluate the changes in the weight of 1,000 kernels, yield, and contents of proximate and bioactive compounds in Chuchung, a mid-late maturing variety, during the pre-harvest maturation of rough rice and to research the appropriate harvest time and potent bioactivity of Chuchung GR. The weights of 1,000 kernels of Chuchung GR dramatically increased until 27 days after heading (DAH). The yields of Chuchung GR declined after 27 DAH and significantly declined to 0.0% after 45 DAH. The caloric value and total mineral contents were higher in the GR than in the full ripe stage, the brown rice (BR). In the GR, the contents of bioactive compounds, such as ${\gamma}$-aminobutyric acid, ${\gamma}$-oryzanol, and ${\alpha}$-tocopherol, were much higher (P<0.05) than those in the BR, specifically during 24~27 DAH. Therefore, bioactive Chuchung GR can be produced with a reasonable yield at 24~27 DAH and it could be useful for applications in various nutritive and functional food products.

수종(數種) 수복물(修復物)의 색소침투(色素浸透)에 관(關)한 실험적(實驗的) 연구(硏究) (AN EXPERIMENTAL STUDY ON PENETRATION OF DYE IN FILLING MATERIALS)

  • 이명종
    • Restorative Dentistry and Endodontics
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    • 제4권1호
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    • pp.29-34
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    • 1978
  • The purpose of this study was to measure penetration of dye stuff(5% Methylene blue, Hematoxylin, Crystal violet and Safranin-O) on silicate cement, Adaptic, Hi-pol and unfilled resin. Each filling material was mixed on the mixing pad and the mixed material was inserted with condensation force of 500gr, 1000gr and 2000gr and without condensation force into preformed glass tube (10mm in diameter and 10mm in height). The specimen was stored in the air for 24 hours, then specimen was immersed in various dye solution (5% methylene blue, hematoxin, crystal violet and safranin-O) for different period of time (l hour and 24 hours). These dye-treated specimen was cut horizontally at the middle portion and the dye penetration in cut surface was measured. Following results were obtained. 1. Pentration of various dye was excessive in silicate cement with and without Condensation force. 2. There has been no evidence of dye penetration in unfilled resin. 3. Dye penetration occurced with in 1 hour period and the extending time didn't affect the dye penetration.

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Simultaneous Voltammetric Determination of Mefenamic Acid and Paracetamol using Graphene Nanosheets/Nickel Oxide Nanoparticles Modified Carbon Paste Electrode

  • Naeemy, Ali;Gholam-Shahbazi, Rozhina;Mohammadi, Ali
    • Journal of Electrochemical Science and Technology
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    • 제8권4호
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    • pp.282-293
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    • 2017
  • A new modified carbon paste electrode (CPE) was constructed based on nickel oxide nanoparticles (NiONPs) and graphene nanosheets (Gr) for simultaneous determination of paracetamol (PCM) and mefenamic acid (MFA) in aqueous media and pharmaceutical dosage forms. NiONPs were synthesized via a simple and inexpensive technique and characterized using X-ray diffraction method. Scanning electron microscopy was used for the characterization of the morphology of modified carbon paste electrode (NiONPs/Gr/CPE). Voltammetric studies suggest that the NiONPs and Gr provide a synergistic augmentation that can increase current responses by improvement of electron transfers of these compounds on the NiONPs/Gr/CPE surface. Using cyclic voltammetry, the NiONPs/Gr/CPE showed good sensitivity and selectivity for the determination of PCM and MFA in individually or mixture standard samples in the linear range of $0.1-30{\mu}g\;mL^{-1}$. The resulted limit of detection and limit of quantification were 20 and $60ng\;mL^{-1}$ for PCM, 24 and $72ng\;mL^{-1}$ for MFA, respectively. The analytical performance of the NiONPs/Gr/CPE was evaluated for the determination of PCM and MFA in pharmaceutical dosage forms with satisfactory results.

Neural Mechanisms Underlying Antidepressant-Like Effects of Glycyrrhizae Radix in Rats

  • Park, Hyun-Jung;Shim, Hyun-Soo;Shim, In-Sop;Lee, Tae-Hee
    • 동의생리병리학회지
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    • 제24권6호
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    • pp.1053-1058
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    • 2010
  • Glycyrrhizae radix (GR) is an herbal medicine commonly used in East Asia for treating a variety of diseases, including stomach disorders. In this study, the antidepressant-like activity of GR was investigated using the forced swimming test (FST) in rats. After the FST, the expression of c-Fos and corticotrophin releasing factor (CRF) was assessed by immunochemistry of brain samples from the paraventricular nucleus of the hypothalamus (PVN). The results of the FST showed that a high dose (400 mg/kg) of extract was very effective in reducing immobility(P<0.01), and increased climbing. In addition, treatment with GR (400 mg/kg) significantly decreased the expression of c-Fos and CRF in the PVN, compared to controls. In conclusion, the findings of this study demonstrated that GR effectively reduced behavioral and physiological depression responses in an animal model of depression, suggesting that GR might be useful in the treatment of clinical depression.