• The Korean Journal of Physiology and Pharmacology
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• v.16 no.5
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• pp.361-365
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• 2012

Anti-tumor activity of the proteins from Gecko (GP) on cervical cancer cells, and its signaling mechanisms were assessed by viable cell counting, propidium iodide (PI) staining, and Western blot analysis. GP induced the cell death of HeLa cells in a dose-dependent manner while it did not affect the viability of normal cells. Western blot analysis showed that GP decreased the activation of Akt, and co-administration of GP and Akt inhibitors synergistically exerted anti-tumor activities on HeLa cells, suggesting the involvement of PI3-kinase/Akt pathway in GP-induced cell death of the cancer cells. Indeed, the cytotoxic effect of GP against HeLa cells was inhibited by overexpression of constituvely active form of Akt in HeLa cells. The candidates of the functional proteins in GP were analyzed by Mass-spectrum. Taken together, our results suggest that GP elicits anti-tumor activity against HeLa cells by inhibition of PI3-kinase/Akt pathway.

• Advances in concrete construction
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• v.10 no.5
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• pp.443-454
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• 2020

In recent years, many studies have been done on the performance of concrete containing glass powder (GP). For the purpose of widespread use of GP in concrete mixes, a knowledge of the performance of such a mixture after a fire is essential for the perspective of structural use. This research work was carried out to evaluate the performance of High Performance Concrete (HPC) made with GP after being exposed to elevated temperature. The studied mixtures include partial replacement of cement by GP with up to 30%. The mechanical performance and structural alterations were assessed after high temperature treatment from 200℃ to 800℃. The mechanical performance was evaluated by testing the specimens to the compressive and tensile strength. In addition, the mass loss and the porosity were measured to notice the structural alterations. Changes in microstructure due to temperature was also investigated by the X-ray diffraction (XRD) and thermal gravimetric analyses (TGA) as well as porosity adsorption tests. The results of the concrete strength tests showed a slight difference in compressive strength and the same tensile strength performance when replacing a part of the cement by GP. However, after high temperature exposition, concrete with GP showed better performance than the reference concrete for temperature below 600℃. But, after heating at 800℃, the strength of the concrete with GP drop slightly more than reference concrete. This is accompanied by an important increase in mass loss and water porosity. After the microstructure analysis, no important changes happened differently for concrete with GP at high temperature except a new calcium silica form appears after the 800℃ heating.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.22 no.5
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• pp.431-440
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• 2019

Purpose: No national survey has yet described the guidelines followed by Korean pediatricians to treat acute gastroenteritis (AGE). An online survey was performed to investigate the management of AGE followed by members of The Korean Society of Pediatric Gastroenterology, Hepatology, and Nutrition, and the results were compared between pediatric gastroenterologists (PG) and general pediatricians (GP). Methods: Questionnaires were sent to pediatricians between June 2 and 4, 2018 regarding the type of hospital, indications for admission, antiemetic and antidiarrheal drugs and antibiotics prescribed, and dietary changes advised. Results: Among the 400 pediatricians approached, 141 pediatricians (35.3%) responded to the survey. PG comprised 39% of the respondents and 72.7% worked at a tertiary hospital. Both PG and GP considered diarrhea or vomiting to be the primary symptom. The most common indication for hospitalization was severe dehydration (98.8%). Most pediatricians managed dehydration with intravenous fluid infusions (PG 98.2%, GP 92.9%). Antiemetics were prescribed by 87.3% of PG and 96.6% of GP. Probiotics to manage diarrhea were prescribed by 89.1% of PG and 100.0% of GP. Antibiotics were used in children with blood in diarrheal stool or high fever. Dietary changes were more commonly recommended by GP (59.3%) than by PG (27.3%) (p<0.05). Tests to identify etiological agents were performed primarily in hospitalized children. Conclusion: This survey assessing the management of pediatric AGE showed that the indications for admission and rehydration were similar between GP and PG. Drug prescriptions for diarrhea and dietary changes were slightly commonly recommended by GP than by PG.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.307-314
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• 2009

This experiment was conducted to investigate the effects of dietary garlic powder (GP) on growth performance and mRNA expression of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) in broiler chickens. Day-old male chicks (Ross, n=270) were fed diets containing 0% [positive control (PC) with antibiotics or negative control (NC) without antibiotics], 1%, 3% and 5% GP for 6 wks. There were no significant differences in feed intake among the treatments throughout the experimental period. Body weight gains in groups fed dietary GP at 3% and 5% were significantly higher than that of NC group (P<0.05). Feeding GP up to 5% did not exert any adverse effect on weight gain and feed intake. There were no significant differences in the relative weights of liver, spleen, cecum and breast muscle. The content of meat cholesterol in GP containing dietary groups tended to be reduced as compared to NC group. Average infectious bronchitis antibody titers in chicks fed GP containing diets were significantly higher than that of NC group (P<0.05). The mRNA expression of hepatic HMG-CoA reductase was reduced by dietary GP. These results indicate that dietary GP has growth promoting effects and tended to alter cholesterol metabolism in broiler chickens.

• Journal of Dairy Science and Biotechnology
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• v.17 no.1
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• pp.1-10
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• 1999

A bacteriocin produced by Lab. acidophilus GP4A isolated from fecal contents of pig was characterized. Lab. acidophilus GP4A produced a heat-stable and pH-resistant bacteriocin, which was hydrolyzed by trypsin and pepsin and active against various microorganisms. Lab. acidophilus GP4A produced bacteriocin at maximum rate when grown in MRS broth(pH 6.5${\sim}$7.5) at$37^{\cric}C$ or $40^{\cric}C$. The bacteriocin produced by Lab. acidophilus GP4A inhibited the growth of Lactobacillus delbrueckii subsp. lactis 4794 in early logarithmic phase. The bacteriocin was purified by ammonium sulfate precipitation and Octyl sepharose CL-4B column chromatography. The purification resulted in a final yield of 21.7% and a 13.6-fold increase in the specific activity.

• Journal of the Korean institute of surface engineering
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• v.54 no.5
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• pp.238-247
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• 2021

PEO (plasma electrolytic oxidation) was applied to modify the surface of AZ31 magnesium alloy in this study. The mixed solution of sodium hydroxide (NaOH) and sodium silicate (Na₂SiO₃) was used as the electrolyte, and 0 - 0.05 g/L of Ca-GP (Glycerol Phosphate Calcium salt) was added in the electrolyte as an additive. PEO treatment was conducted at a current density of 30mA/cm² for 5 minutes using a DC power supply. The surface properties were identified by SEM, XRD and surface roughness analyses, and the corrosion resistance was evaluated by potentiodynamic polarization and immersion tests. In addition, the biocompatibility was evaluated by immersion test in SBF solution. As the concentration of Ca-GP was increased, the surface morphology was denser and more uniform, and the amount of Ca and the thickness of oxide layer increased. Only Mg peak was observed in XRD analysis due to very thin oxide layer. The corrosion resistance of PEO-treated samples increased with the concentration of Ca-GP in comparision with the untreated sample. In particular, the highest corrosion resistance was identified at the group of 0.04g Ca-GP through potentiodynamic polarization and immersion tests in saline solution (0.9 wt.%NaCl). During the immersion in saline solution, pH rapidly increased at the beginning of immersion period due to rapid corrosion, and then increase rate of pH decreased. However, the pH value in the SBF temporarily increased from 7.4 to 8.5 during the day, then decreased due to the inhibition of corrosion with HA(hydroxyapatite) formation.

• Magazine of the Korean Society of Agricultural Engineers
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• v.41 no.6
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• pp.90-96
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• 1999

Over 4.1 billion metric tons of non-hazardous solid wstes are generated in the United States annually. Therefore, there is a need to reuse or reprocess as many products or by-products as possible. In this report, theaggregates produced from Naval Shipyard and Charleston area dredge spoils are designated as GP-21-23 and GP 4-5, repectively. The major objective of this study is to determine the feasiblity of the use of these aggregates in concrete. The followings are some of the conclusions, observations and recommendations regarding this portion of the research project. In most cases, the unit weight of concrete containing GP 21-23 and GP 4-5 (vitrified galss) lower than the control concrete, and the concrete containing vitrified glass produced lower compressive and bending strength compared to the control. However the concrete containing virtrified glass could be used in the field based on the minimum criterial set for the mix design. The use of vitrified glass in concrete is recommended in several applications except parking lots, side walks and main roads, because abrasion resistace is not high.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1262-1269
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• 2003

In this study, enzymes were investigated as an antistaling agent for a Korean rice cake. Thermograms by a DSC demonstrated that the gelatinization-onset temperature of the Korean rice cake was at its lowest temperature of 71.1$^{\circ}C$ with the GP (glucoamylase+pullulanase) treatment, followed by $\beta$-amylase and $\alpha$-amylase. The gelatinization peak temperature of the Korean rice cake with enzyme treatment was relatively lower compared to the control. Furthermore, the Korean rice cake with GP treatment showed the lowest peak temperature. Melting enthalpy of the Korean rice cake increased with the enzyme treatment, with $\beta$-amylase, followed by $\alpha$-amylase and GP. Melting enthalpy of the Korean rice cake with GP treatment was significantly lower compared to the $\beta$- and $\alpha$-amylase treatment. Recrystallinity in the case of GP treatment was also significantly lower than control. The range of Avrami exponent (n) was 0.90 ∼ 1.20 and the time constant of retrogradation (1/k) of the Korean rice cake crystalline decreased in the following order: GP, $\beta$-, $\alpha$ -amylase and control. Textural characteristics of the Korean rice cake with enzyme treatment differed greatly from that of control. The L＊ values of all the Korean rice cakes made without $\beta$-amylase decreased and the a＊ values were significantly different at p<0.05. The GP treatment altered the b＊ value toward blue color, whereas $\beta$-and $\alpha$-amylase changed to the direction to yellow color. In sensory evaluation, the Korean rice cake with enzyme treatment showed higher evaluation compared to control.

• Journal of Pharmaceutical Investigation
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• v.40 no.6
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• pp.363-366
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• 2010

Identification of compounds that function as P-glycoprotein (P-gp) substrates or inhibitors can facilitate the selection and optimization of new drug candidates. The purpose of this study is to optimize the experimental conditions for in vitro P-gp assay using LLC-GA5 cells, which is a well-known transformant cell line derived by transfecting LLC-PK1 with human MDR1. The amount of rhodamine123 transported by the LLC-GA5 and LLC-PK1 cells was evaluated under the following experimental conditions: 3 different types of transport media, colchicine pretreatment or nontreatment of the cells in the culture media, and with and without poly-L-lysine coating of the culture plates. The assay sensitivity was found to considerably differ depending on the diluents used in the transport media. P-gp-mediated transport in LLC-GA5 cells was most clearly characterized in the Hanks' balanced salt solution based transport media. The sensitivity of P-gp-mediated transport was not changed by colchicine pretreatment or poly-L-lysine coating of the culture plates.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.1003-1014
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• 2022

This study investigated quality characteristics of chicken stock with added plum extract (PE) and green tea powder (GP) stock during storage. Plum extract (0, 0.5, 1, 3%) and green tea powder (0, 0.5, 1, 3%) were added at three levels. Chicken stock was stored at room temperature for 14 days. The pH of the chicken stock decreased significantly as the content of PE and GP increased (p < 0.05). The group with 3% plum extract added showed significantly lower pH values (p < 0.05). Total numbers of microorganisms (TMC) showed significant differences according to the storage period (p < 0.05), where the groups with PE and GP added showed lower TMC values than the control group, This indicates that PE and GP could inhibit the growth of microorganisms. The addition of 3% PE or GP decreased lipid oxidation (TBARS) and protein deterioration (VBN) values. In sensory test, the results showed that adding PE or GP has a positive effect on storage characteristics of chicken stock. The addition of PE rather than GP is effective not only in improving sensory evaluation, but also in minimizing changes in quality by suppressing lipid oxidation and protein deterioration during storage. In conclusion, 3% PE addition was found to be the most optimal supplementation choice for increasing the storability of chicken stock.