• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2355-2359
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• 2013

Vulva cancer is rare among all gynecological cancer worldwide, including Thailand, and mainly affects older women. Persistent high risk type infection of human papillomavirus (HPV) is the one important factor for developing cancer. In this study, we focused on HPV DNA investigation and type-specific distribution of HPV in 25 formalin-fixed paraffin-embedded (FFPE) samples collected from Thai women with vulva cancer histologically confirmed by the National Cancer Institute, Thailand, during 2003-2011. HPV DNA detection and genotyping were undertaken with polymerase-chain reaction and enzyme-immunoassay using GP5+/bio6+ consensus specific primers and digoxigenin-labeled specific oligoprobes, respectively. Human ${\beta}$-globin genes was used as the internal control. Our results showed that 44% (11/25) of all vulva cancer samples were HPV-positive. All of them are high risk HPV type infection, detected as single (63.64%, 7/11) and/or double infections (4/11, 36.36%). HPV 16 was the most common type identified in vulva cancer, followed by HPV 35, 33, 18 and 58. In conclusion, this study presented that HPV-16 is observed at the highest frequency in this cancer, similar to cervical cancer, with HPV 18 being less frequent. Although the sample size was small and could not represent overall incidence and prevalence in Thai women, these preliminary data for vulva cancer are of interest since they reinforce the necessity for HPV screening or vaccination in Thailand.

• Journal of Acupuncture Research
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• 제20권5호
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• pp.50-62
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• 2003

Objective: Bee Venom(BV) has been used for various kinds of inflammatory or painful conditions in Oriental Medicine clinics, and there publishes reports on its therapeutic effects and the probable mechanism of those therapeutic effects, where CDs and cytokines plays important role. This study investigated the influences of bee venom on the expressions of CDs and cytokines of HMC cell line Methods: In this study we analysed the expression profile of HMC cell line treated with BV of 10-2ug/ml in relation to that of HMC cell line treated with vehicle by way of CD/cytokine microarray hybridization with 342 genes on it. Results: There were no upregulated genes by more than 3 fold, while there showed some downregulated genes by less than 1/3 fold as follows: colony stimulating factor 2, CD122, IL-7, CD112, TNF-alpha, CD138, CD166, TGFbetaR2, CD42b, CD62L, CD111, interleukin 10 receptor alpha, colony stimulating factor 1(macrophage), CD38 antigen(p45), CD121a, CD33 antigen(gp67), colony stimulating factor 1 receptor, B cell linker protein (SLP65) mRNA, CD94, alanyl(membrane) aminopeptidase, immunoglobulin(CD79A) binding protein 1, CD205, CD241, CD207, CDw121b, integrin alpha L(CD11a), integrin beta 1(CD29), CD91, CD42b. Conclusions: Bee venom treatment induced downregulation of some CDs or cytokines including $TNF-{\alpha}$. IL-1R with its possible implication in an antiinflammatory action of BV. Further research on expression profile changes induced by BV treatment is expected.

• Bulletin of the Korean Chemical Society
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• 제27권10호
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• pp.1618-1622
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• 2006

Bacteriophage T7 gp4A' is a ring-shaped hexameric DNA helicase that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. To investigate the effect of single-stranded DNA (ssDNA) on the kinetic pathway of dTTP hydrolysis by the T7 DNA helicase complexed with ssDNA, we have first determined optimal concentration of long circular M13 single-stranded DNA and pre-incubation time in the absence of $Mg^{2+}$ which is necessary for the helicase-ssDNA complex formation. Steady state dTTP hydrolysis in the absence of $Mg^{2+}$ by the helicase-ssDNA complex provided $k_{cat}$ of $8.5\;{\times}\;10^{-3}\;sec^{-1}$. Pre-steady state kinetics of the dTTP hydrolysis by the pre-assembled hexameric helicase was monitored by using the rapid chemical quench-flow technique both in the presence and absence of M13 ssDNA. Pre-steady state dTTP hydrolysis showed distinct burst kinetics in both cases, indicating that product release step is slower than dTTP hydrolysis step. Pre-steady state burst rates were similar both in the presence and absence of ssDNA, while steady state dTTP hydrolysis rate in the presence of ssDNA was much faster than in the absence of ssDNA. These results suggest that single-stranded DNA stimulates dTTP hydrolysis reaction by T7 helicase by enhancing the rate of product release step.

• 한국측량학회지
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• 제26권5호
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• pp.475-483
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• 2008

본 연구의 목적은 국내 측량관련 전문학회의 학회지, 석 박사 학위논문을 대상으로 하여 논문분야별, 연도별 분석을 통하여 측량분야의 연구동향을 파악하는데 있다. 본 연구에서는1957년 이후 현재까지의 국내에서 발표된 측량관련 전문학술지 발표논문, 석 박사 학위논문을 취합 정리하였다. 연구 동향을 연도별과 분야별로 그 현황을 분석하였으며 각 연구 분야에 사이의 상관관계를 분석한 결과 원격탐사 분야와 GIS 분야가 매우 높은(약 98%) 상관관계를 가지고 있었으며, 그 다음으로는 GPS와 원격탐사 분야가 높은(약 95%) 상관관계를 가지고 있음을 알 수 있었다. 각 논문분야의 발표연도변화에 따른 회귀분석 결과 측지분야를 제외하면 대부분 추세선이 대부분 3차식에 유의함을 알 수 있었다. 또한, 일반측량, 사진측량, 원격탐사, 토지정보 분야의 논문은 증가하는 것으로 예상되며, GPS, GIS, 수치지도, 측지분야는 감소하는 것으로 예상됨을 알 수 있었다.

• Nutrition Research and Practice
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• 제4권2호
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• pp.114-120
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• 2010

The effects of grape seeds extract and grape peels extract prepared from grape pomace on the activity of antioxidant enzymes, degree of lipid peroxidation in serum and liver tissue were investigated in rabbits fed on high cholesterol diet. New Zealand white rabbits were divided as follows ; 1) NOR (normal group); 2) CHOL (cholesterol group); 3) GSH (cholesterol + grape seed extract group); 4) GPE (cholesterol + grape peel extract); 5) GSP (cholesterol + grape seed powder); 6) GPP (cholesterol + grape peel powder); 7) GE (cholesterol + grape seed and peel extract); 8) GP (cholesterol + grape seed and peel powder). Eight groups of rabbits were studied for 8 weeks. At the end of the experimental period, rabbits were sacrificed and the liver tissue were removed. Then, GSH, GPx, GST, CAT and MDA in the liver were measured. In liver tissues, total glutathione contents (GSH), glutathione peroxidase (GPx) and catalase (CAT) activity, which was significantly higher by grape seed extract supplementation. The level of malondialdehyde (MDA) was lower in the serum of rabbits fed grape seed extract or grape peel powder plus cholesterol than in the serum of rabbits fed cholesterol alone. It is therefore likely that grape seed extract prepared from grape pomace functioned as antioxidants in vivo, negating the effects of the oxidative stress induced by 1% cholesterol diet. The grape seed extract was found effective in converting the oxidized glutathione into reduced glutathione, and in removing $H_2O_2$ that is created by oxidative stress. The grape peel powder was found to have small influence on reduced glutathione content, CAT and GPX activity, but it increased GST activity in liver tissues, resulting in promoting the combination of lipid peroxide and glutathione (GSH), and further, lowering the formation of lipid peroxide in the serum. Therefore, grape pomace (grape seed extract and grape peel powder) supplementation is considered to activate the antioxidant enzyme system and prevent damage with hypercholesterolemia.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.1022-1026
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• 2010

The different cleavage patterns of pYBamy59 plasmid isolated from E. coli $DH5{\alpha}$ and B. longum MG1 by the cell extract of B. longum MG1 suggested that the main reason for its low transformation efficiency was related to the restriction modification (R-M) system. To confirm the correlation between the R-M system and transformation efficiency, in vitro methylation and site-directed mutagenesis were performed in pYBamy59. Sequence analysis of pYBamy59 fragments digested by the cell extract of B. longum MG1 revealed that all fragments were generated by restriction of the sequence recognized by SacII endonuclease. When pYBamy59 from E. coli was methylated in vitro by CpG or GpC methyltransferase, it was protected from SacII digestion. Site-directed mutagenesis, which removed SacII sites from pYBamy59, or in vitro methylation of pYBamy59 showed 8- to 15-fold increases in the transformation efficiency over intact pYBamy59. Modification of the SacII-related R-M system in B. longum MG1 and in vitro methylation in pYBamy 59 can improve the transformation efficiency in this strain. The results showed that the R-M system is a factor to limit introduction of exogenous DNA, and in vitro modification is a convenient method to overcome the barrier of the R-M system for transformation.

• Journal of Astronomy and Space Sciences
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• 제16권1호
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• pp.61-68
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• 1999

GPS신호는 대류권을 지나면서 지연된다. 대류권에서의 신호지연은 건조공기에 의한 건조지연과 수증기에 의한 습윤지역으로 분리할 수 있다. 본 연구에서는 ’98년 5월 한달동안 대전, 수원에서 관측된 GPS자료를 최소자승법으로 처리하여 천장방향에 대한 총 대류권 신호지연값을 얻어내었다. 건조지연값은 지상기압을 정확하게 관측하면 얻어지므로 총대류권 지연양에서 건조지연값을 빼주면 천장방향의 습윤지연값을 얻게된다. 천장 습윤지연값은 대류층의 수증기양과 직접적으로 연관됨으로 수증기량을 측정하였고 결과의 신빙성을 검증하기 위해 오산에서 관측된 라디오존데 자료를 이용하여 계산한 대류권 수증기량과 비교하여 보았다. 그 결과 오산에서 가까운 수원의 경우 수증기량의 변화 경향이 라디오존데의 그것과 대체로 일치하였고 표준편차 3.68mm의 정도의 정확도를 보였다.

• 한국정보통신학회논문지
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• 제8권5호
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• pp.1004-1009
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• 2004

컴퓨터 시스템에서의 시각 메커니즘은 기본적이고 필수적인 요소로써 현재 시각의 유지 및 프로세스 점유 시간, 디스크 I/O 등, 시스템의 각종 성능 평가를 위한 척도이다. 분산 시스템에서 응용프로세스들은 시스템 내 여러 노드에서 동시에 수행되며 정확한 결과를 얻기 위해서는 노드 간 시각 동기가 이루어져야 한다. 현재 통신망이 보편적 요소가 되면서 분산 환경 구성 및 네트워크 기반 운용이 일반화됨에 따라 시각 동기는 필수 사항이 되었다. 네트워크 시각 서버는 시스템의 지역 클럭을 표준 참조 시각원에 동기시킴으로써 정확하고 정밀한 시각을 획득, 유지하고 표준 시각 동기 프로토콜을 통해 시각 정보를 네트워크에 분배한다. 본 논문은 네트워크 시각 동기를 위한 시각 서버의 설계 및 구현에 대해 제시한다. 시스템은 표준 참조 시각원으로 GPS (Global Positioning System)를 사용하며 NTP (Network Time Protocol)를 통해 표준시 (UTC: Universal Time Coordinated)를 제공한다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1083-1088
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• 2016

Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4OC and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

• 한국지리정보학회지
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• 제5권4호
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• pp.75-85
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• 2002

GPS 기술이 교통에 도입되면서 차량의 위치에 대한 실시간 추적이 가능하게 되었다. X, Y, Z의 3차원으로 측정되는 위치정보는 시간상으로 연속적일 뿐만 아니라, 2대 이상의 연속주행 차량에 대해서도 동시 측정이 가능하다. 따라서 그간 자료수집의 한계로 인해 제한적으로 진행되던 연구에 많은 도움이 되고 있다. 하지만 GPS를 통해 수집한 자료의 정확성을 규명하는 것이 선행되지 않고는 합리적인 결과를 기대하기는 어려울 것이다. 기본적으로 수반되는 오차의 크기에 따라 연구결과에 대한 신뢰성이 달라질 수 있기 때문이다. 따라서 본 연구의 목적은 GPS 수신기로부터 측정되는 자료의 정확도를 분석 보정함으로서 동일 기종의 장비를 이용하여 수행되는 향후 연구에 대한 준거를 제시하고자 함이다. 연구는 2가지 유형의 구간으로 구분하여 진행하였는데, 대상구간 주변의 3, 4등 삼각점을 설정하고, 이로부터 측정된 측량 좌표와 DGPS 수신자료에 의한 좌표를 비교하였다. DGPS 수신 결과는 최대 0.6m 이내의 오차를 수반하는 것으로 나타났다.