• Journal of Microbiology
• /
• 제42권3호
• /
• pp.169-173
• /
• 2004

Enterobacter cloacae (strain PR2/7), a genetically modified endophyte(GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial- clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.

• 한국운동역학회지
• /
• 제17권4호
• /
• pp.141-148
• /
• 2007

Falls associated with tripping over an obstacle can be dangerous, yet little is known about the strategies used for stepping over obstacles in older Parkinson disease. The purpose of this study was to investigate the lower extremity muscle activity on the obstacle gait according to obstacle height in older Parkinson diseases. The obstacle gait of 7 older Parkinson disease was examined during a 5.0 m approach to, and while stepping over, obstacles of 0, 25, 52, and 152mm. Seven pairs of surface electrodes(Noraxon MyoResearch, USA) were attached to the right-hand side of the body to monitor the adductor longus(AL), gluteus medius(GME), gluteus maximus(GMA), biceps femoris(BF), rectus femoris(RF), gastrocnemius(GA), tibialis anterior(TA). Electromyography data were filtered using a 10Hz to 350 Hz Butterworth band-pass digital filter and normalized to the maximum value in the analyzed phases. A one-way ANOVA for repeated measures was employed for selected electromyography variables to analyze the differences of the height of four obstacles. The results showed significant differences between 0.0mm and 25, 52, and 152mm obstacle height in TA and GA activities during the second phase(swing phase). But the more increase obstacle height, the more not increase the muscle activities. This means that the Parkinson disease stepping over obstacle inefficiency. To prevent and reduce the frequency of falls, elderly Parkinson disease maintained and improved their balance, muscular strength, neuromuscular control and mobility.

• 한국운동역학회지
• /
• 제19권2호
• /
• pp.257-264
• /
• 2009

본 연구의 목적은 파킨슨 환자들에게 일상생활 속에서 빈번히 수행되는 방향전환 동작 중에 작용하는 하지의 운동학 및 근육의 활동을 규명하는데 있다. 호엔야르 $1.5{\sim}4$척도의 60대 파킨슨 환자 7명이 본 실험에 참여하였다. 실험실 바닥에 직선($0^{\circ}$) 및 방향전환($60^{\circ}$, $90^{\circ}$, $120^{\circ}$) 보행로를 만들었다. 보행로에 5cm 넓이의 검은색 접착테이프를 붙여 놓아, 그 경로를 따라 무작위 순서로 지나가도록 하였다. 하지의 운동학적 변인을 측정하기 위해 해당부실에 21개의 마커를 부착하였으며, 근전도 변인을 측정하기 위해 우측 하지의 장내전근, 중둔근, 대둔근, 대퇴이두근, 대퇴직근, 비복근, 전경골근에 전극을 부착하였다. 방향전환 각도별(4수준: $0^{\circ}$, $60^{\circ}$, $90^{\circ}$, $120^{\circ}$)로 얻은 하지의 운동학 및 근전도 변인의 차이를 규명하기 위하여 반복이 있는 일원 변량 분석을 실시하였다. 파킨슨 환자들은 방향전환시 활보장과 보간을 감소시키는 것으로 나타났으며, 하지에 작용하는 근육활동에는 차이가 없는 것으로 나타났다.