• 한국식품과학회지
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• 제46권2호
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• pp.213-218
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• 2014

본 연구에서는 ${\beta}$-carotene 강화쌀의 생체이용율과 영양기능성을 모종쌀과 비교하였다. 실험동물은 수컷 Wistar 흰쥐를 사용하였고, Harlan 2018S-diet를 기본 식이로 하였다. 실험 식이는 낙동쌀(모종쌀), ${\beta}$-carotene 강화쌀(GM쌀) 및 Harlan 2018S-diet(대조군)으로 하였고, 실험군당 11마리씩 배정하였다. 실험식이에 함유된 전분 함량은 모종쌀과 GM쌀로 각각 30% 대체하였고, 펠렛 식이로 재성형하여 4주 동안 급여하였다. 실험기간동안 GM쌀의 급여는 모종쌀과 비교시 흰쥐에게 식이섭취량이나 체중 증가와 같은 일반적인 성장에 부정적인 영향을 주지 않았다. 간, 신장, 비장, 체지방 등의 6개 장기의 무게를 측정한 결과 GM쌀은 모종쌀 식이 섭취군과 비교시 유의적인 차이가 없었다. 간지질중 총콜레스테롤(TC) 함량은 GM쌀 식이군이 모종쌀 식이군 보다 유의하게 낮았고, 분변지질(TC, TG) 농도 역시 GM쌀 식이군이 모종쌀 식이군에 비해 낮았다. 혈청생화학치와 혈구세포의 변화를 살펴보면 전반적으로 두 실험군(모종쌀, GM쌀) 간의 차이가 없었으나, 혈청지질 농도는 GM쌀 식이군이 모종쌀 식이군 보다 낮았고, 공복혈당은 GM쌀 식이군이 모종쌀 식이군 보다 통계적으로 유의하게 낮았다. 위의 결과들을 종합하면 GM쌀은 모종쌀에 비해 생체이용 측면에서 성장률과 장기무게 및 체지방 등에서 차이가 없었고, 영양기능 측면에서는 배변량을 증가시키고, 간과 분변 및 혈중 지질농도를 낮추며, 공복혈당치를 유의하게 감소시키는 것으로 나타났다.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1310-1314
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• 2012

본 실험은 제초제 내성을 가지는 CP4EPSPS를 도입시킨 유전자재조합 콩을 이용하여 인공위액의 비율에 따른 도입 단백질의 소화특성을 평가하고, 열처리가 유전자재조합 콩의 도입단백질의 소화성에 미치는 영향을 살펴보았다. 유전자재조합 콩과 펩신의 비율에 따른 도입단백질 CP4EPSPS의 소화성은 인공위액 즉 펩신의 양에 따라 차이를 나타내 유전자재조합 식품의 도입단백질에 대한 소화성 평가에서 펩신의 양이 중요한 요인이 됨을 알 수 있었다. 또한 유전자 재조합 콩에 대한 예열처리가 도입단백질 CP4EPSPS의 펩신에 대한 내성을 많이 감소시켜 소화성을 높이는 것으로 나타났다. 따라서 앞으로 유전자재조합식품의 안전성을 평가하는 in vitro 소화평가 시험에서 생리학적 측면에 근거한 인공위액의 비율을 반영함으로써 보다 정확한 도입단백질의 소화성에 대한 평가가 필요하리라 사료되며, 또한 콩의 주요 섭취방법인 열처리에 따른 도입단백질의 소화특성을 평가함으로써 유전자재조합 콩의 안전성에 대한 신뢰도 제고를 위한 기초자료가 될 것으로 기대된다.

• Food Science and Biotechnology
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• 제18권3호
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• pp.694-699
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• 2009

A multiplex polymerase chain reaction (PCR) method was developed for the detection of 4 events of genetically modified (GM) soybean. The event-specific primers were designed from 4 events of GM soybean (RRS, A2704-12, DP356043-5, and MON89788). The lectin was used as an endogenous reference gene of soybean in the PCR detection. The primer pair YjLec-4-F/R producing 100 bp amplicon was used to amplify the lectin gene and no amplified product was observed in any of the 9 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM soybean mixture containing RRS, A2704-12, DP356043-5, and MON89788. In this study, 20 soybean products obtained from commercial food markets were analyzed by the multiplex PCR. As a result, 6 samples contained RRS. These results indicate that this multiplex PCR method could be a useful tool for monitoring GM soybean.

• 한국식품조리과학회지
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• 제32권3호
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• pp.261-269
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• 2016

Purpose: The purpose of this study was to investigate the effects of mechanical processing (tumbler, tenderizer, injector) and Ganghwa mugwort extracts (GM) on the stability of chicken Neobiahni during storage for 10 days at $4^{\circ}C$. Six treatments of chicken Neobiahni were manufactured with the following conditions: CON (tumbler), CON-A (tumbler + 0.2% GM), T1(tenderizer) T1-A (tenderizer + 0.2% GM), T2 (injector), T2-A (injector + 0.2% GM). Methods: The pH, POV, TBA, and sensory characteristics of chicken Neobiahni during storage for 10 days at $4^{\circ}C$ were measured in triplicate. Results: The pH of chicken Neobiahni was in the range of 6.00-6.37, with the highest values in the treatments containing GM (CON-A, T1-A, T2-A). Mechanical processing had no significant effects during storage. The color values (lightness, redness, and yellowness) did not differ significantly in all chicken Neobiahni samples, whereas storage time had a significant effect (p<0.05). The mechanical processing combined with GM appeared to effectively control the POV and TBA levels of chicken samples during the entire storage period. In addition, sensory evaluation ratings (color, juiciness, flavor, tenderness, and overall acceptability) were improved by the mechanical processing and the addition of GM. Conclusion: These results suggest that the combination of mechanical processing and Ganghwa mugwort extracts is a useful technique for retarding lipid oxidation in chicken Neobiahni.

• 한국식품저장유통학회지
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• 제22권6호
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• pp.901-907
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• 2015

CMV 바이러스 저항성 H15 고추 도입유전자의 발현산물인 CMV-CP 단백질의 80개씩의 아미노산 비교에서 35% 보다 큰 상동성을 갖거나 8개 이상의 일련된 아미노산 염기 서열이 일치하는 알레르겐은 존재하지 않는 것이 확인되었으며, 따라서 CMV-CP 단백질 서열은 기지의 알레르겐과 구조적 및 면역학적으로 연관된 유사성이 없음을 확인하였다. 형질전환에 따른 특성 변화를 비교하기 위해 서로 다른 지역에서 재배된 CMV 바이러스 저항성 H15 고추 및 그 모본의 발현 단백질 항원 농도와 그 분포를 비교한 결과 발현된 단백질의 양상과 양은 재배 연도 및 환경적 영향에 따른 차이가 발견되었지만, 동일한 연도에 같은 장소에서 재배된 유전자변형 고추와 그 모본 사이에서의 차이는 발견되지 않아 형질전환으로 인한 특성변화는 없는 것으로 확인 되었다. 환자 혈청을 이용해 immunoblotting법과 ELISA법으로 확인한 항원-항체 반응성 비교에서도 CMV 바이러스 저항성 H15 고추와 그 모본 P2377사이에 차이가 없는 것이 확인되었다. 이러한 결과를 종합할 때, 유전자변형 고추 H15는 그 모본과 비교하여 형질전환으로 인한 알레르기성의 변화는 없는 것으로 판단된다.

• 원예과학기술지
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• 제34권6호
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• pp.924-939
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• 2016

The great economic losses caused by Cucumber mosaic virus (CMV) infection of peppers has led to the development of genetically modified (GM) CMV-resistant peppers. We developed virus-resistant pepper plants using Agrobacterium tumefaciens -mediated transformation. The expressed recombinant protein was purified using nickel-nitrilotriacetic acid resin and immunoaffinity chromatography, and purity was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoblot analysis revealed the purified CMV coat protein (CMV-CP) had a molecular mass of 25 kDa. After in-gel digestion and desalting, the internal peptide fragments of CMV-CP were sequenced by matrix-assisted laser desorption/ionization-time of flight. Most GM pepper and Escherichia coli BL21 internal peptides had identical peptide sequences and contained 137 of 183 whole peptides in CMV-CP. A quantitative enzyme-linked immunosorbent assay was performed to detect CMV-resistant GM peppers. We also provide basic information about the expressed protein in GM peppers for further safety assessment. The contents of soluble protein and CMV-CP were measured in GM and control peppers cultivated in three different areas of Korea. Statistical significance in terms of cultivation areas, harvest times, generations, and plant tissue origin were determined based on a P value of 0.05. The highest amount of CMV-CP was detected at the seedling stage from plant grown in each region. T3 and T5 showed significantly different levels of CMV-CP from T4 in leaves in the whorl stage. No statistical differences were observed among GM peppers at different stages of maturity in any cultivation area. The results from this study contribute to the safety evaluation of newly designed CMV-resistant GM peppers and provide a standard against which to compare other virus-resistant GM peppers.

• 한국식품과학회지
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• 제36권5호
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• pp.828-832
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• 2004

본 연구에서는 유전자 재조합 되지 않은(non-GM) 대두와 유전자 재조합된(GM) 대두가 혼입되어 제조된 두부에서 효소 면역 측정법을 이용하여 non-GM 대두의 혼입량을 추정하고자 하였다. 두부의 SDS-PAGE 실행 결과 non-GM 두부에서만 나타나는 특이 단백질 non-GM 113kDa 밴드와 non-GM과 GM 두부에서 모두 나타나는 non-GM 24kDa 밴드를 선별하고 이들을 토끼에 면역하여 항체생성 여부를 ELISA한 결과 non-GM 113kDa과 non-GM 24kDa 단백질 모두 항체가 형성됨을 확인하였고 $10^{-1}-10^{-6}$의 단백질 희석배수에서 두부를 이들 항체에 대하여 ELISA함으로써 원료대두의 GM여부를 확인할 수 있었다. 이들 중, 보다 감도가 높았던 non-GM 113kDa 단백질을 $10^{-7}-10^{-6}$의 배수로 희석하여 ELISA 흡광도와 non-GM 단백질의 관계를 나타내는 표준곡선을 작성하였고, 임의로 non-GM 대두와 GM 대두를 혼합하여 제조한 두부의 ELISA 흡광도를 이 표준곡선과 비교하여 non-GM 원료와 GM 원료 작물의 혼입율을 측정한 결과, 높은 정확도를 보였다.

• Food Science and Biotechnology
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• 제17권4호
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• pp.829-832
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• 2008

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

• Journal of Nutrition and Health
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• 제16권3호
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• pp.162-184
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• 1983

The sodium amuounts of 35 food items and of the city supplied tap water in Seoul area were analyzed ay the Atomic Absorption Spectrophotometry. The Korean food exchange lists for the sodium resricted diets were developed from the available data and the ones newly obtained in this research. The food exchange lists provided in this research is compiled from (1) Milk group (2) Vegetable groups : A with the carbhydrate content of 0-4.9% and -B with that of 5.0-14.9% (3) Fruit group (4 ) Grains and starch food group (5) Meat groups : -Low fat meat and protein foods with the fat content of 0-3.0gm ; -Medium fat meat and protein foods with that of 5.0gm and : -High fat meat and protein foods with that of 8.0gm and (6) Fat group. Lists of sweets, alcoholic and nonalcoholic beverages and seasonings and condiments were also provided with the amount of sodium they contain in portions commonly used. The research described in this report was supported by the Grant from the Department of Education.

• 한국식품위생안전성학회지
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• 제9권3호
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• pp.123-131
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• 1994

An enzyme-linked immunosorbent assay(ELISA) was developed for the detection of residual gentamicin(GM) in edible animal products. The immunogen(GM-KLH conjugate) and coating antigen(GM-BSA conjugate) were prepared by coupling GM sulfate to keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, respectively. Polyclonal antibody to GM was produced in rabbits(New Zealand White, female) by using the immunogen and the antibody titer was measured by indirect ELISA. A competitive ELISA was developed using GM-bovine serum albumin conjugate as a coating antigen, GM(as standards or sample), polyclonal antibody to GM, secondary antibody conjugated with horseradish peroxidase as an enzyme, and H2O2 and o-phenylenediamine dihydrochloride as a substrate and a chromophore, respectively. The detection limit of GM was 10 ng/ml and the standard curve of GM(n=26) was linear up to 10 $\mu\textrm{g}$/ml in this competitive ELISA system. There were no cross-reactivities of the partially purified antibody between GM and the various antibiotice such as amikacin, benzyl-penicillin, chloramphenicol, erythromycin, furazlidone, kanamycin, neomycin, oleandomycin, streptomycin, sulfathiazole and thiamphenicol(CR50<0.05%)