• 대한원격탐사학회지
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• 제37권6_2호
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• pp.1829-1836
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• 2021

구름탐지란 위성영상내의 픽셀 혹은 화소에서의 구름 유무를 결정하는 것을 의미하며 해당 위성영상의 활용성과 정확도에 영향을 미치는 중요한 요소로 작용한다. 본 연구에서는 구름탐지 자료를 제공해주는 여러 선진기관들의 위성 중에서, GK-2A(GeoKompsat-2A)/AMI(Advanced Meteorological Imager)와 Terra(Earth Observation System-Terra)/MODIS(Moderate-Resolution Imaging Spectroradiometer), Suomi-NPP(The Suomi National Polar-orbiting Partnership)/VIIRS(Visible Infrared Imaging Radiometer Suite)의 구름탐지 자료의 차이에 대해서 정량적 및 정성적으로 비교 분석을 수행하고자 한다. 정량적으로 비교한 결과 1월의 Proportion Correct(PC)지수 값이 GK-2A & MODIS가 74.16%, GK-2A & VIIRS가 75.39%를 나타냈으며 4월의 GK-2A & MODIS는 87.35%, GK-2A & VIIRS는 87.71%로 4월이 1월보다 위성별로 큰 차이 없이 구름을 탐지한 것으로 나타났다. 정성적 비교 결과는 RGB영상과 비교하였을 때, 앞선 정량적 결과들의 경향과 동일하게 1월보다 4월에 해당하는 결과들이 구름을 잘 탐지한 것을 확인할 수 있었으나 얇은 구름이나 적설이 존재하는 경우에는 위성별로 구름탐지 결과에 다소 차이가 존재하였다.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.298-304
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• 1997

Thermus caldophilus GK24 was selected as sources of thermostable $\beta$-galactosidase from a survey of genus Thermus. T. caldophilus GK24 (Tca) $\beta$-galactosidase was found to be inducible. The enzyme was optimally active at 75$\circ$C. Enzyme induction was achieved by addition of lactose, galactose and cellobiose to basal media. The addition of glucose to culture media had a repressive effect on further enzyme synthesis. T caldophilus GK24 was tested for production of $\beta$-galactosidase by addition of various concentration of lactose, galactose and cellobiose to standard media. Cellobiose was found to be effective for the $\beta$-galactosidase induction. The optimal induction medium for production of $\beta$-galactosidase was composed of 0.2% cellobiose, 0.3% bactotryptone, 0.3% yeast extract, basal salts and Tris/HCI(pH 7.8). The activity of the enzyme in the optimal induction medium increased nearly 16.5-fold compared to the standard medium. Tca $\beta$-galactosidase was detected when cell extracts was subjected to electrophoresis in a nondenaturing polyacryamide gel and stained for activity with 6-bromo-2-naphtyl-$\beta$-D-galactopyranoside(BNG).

• 한국축산식품학회지
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• 제42권6호
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• pp.928-941
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• 2022

This study aimed to analyze the microbiological composition and sensory characterization of fermented sausage using strains isolated from Kimchi (GK1, Pediococcus pentosaceus SMFM2016-GK1; NK3, P. pentosaceus SMFM2016-NK3), Doenjang (D1, Debaryomyces hansenii SMFM2021-D1), and spontaneously fermented sausage (S8, D. hansenii SMFM2021-S8; S6, Penicillium nalgiovense SMFM2021-S6). The control was commercial starter culture. Nine treatments were applied [GD (GK1+D1), GS (GK1+S8), GDS (GK1+D1+S8), ND (NK3+D1), NS (NK3+S8), NDS (NK3+D1+S8), GND (GK1+NK3+D1), GNS (GK1+NK3+S8), and GNDS (GK1+NK3+D1+S8)] by mixing lactic acid bacteria and yeast, and S6 was sprayed. The microbial composition of fermented sausage was analyzed [aerobic bacteria (AC), Lactobacillus spp. (LABC), Staphylococcus spp. (STPC), and yeast and mold (YMC)], and pH and electronic nose and tongue measurements were taken. The AC, LABC, STPC, and YMC values of the control and treatment groups tended to increase during fermentation (p>0.05). The STPC values of the GD, GS, ND, and GDS groups were similar to that of the control on day 3. The pH of the control on day 3 was significantly lower than that of the GD, ND, and GND groups (p<0.05). Higher levels of 4-methylpentanol, 2-furanmethanol, and propyl nonanoate, which provide a "fermented" flavor, were detected in the GD group compared to in the control and other treatment groups. GD and ND groups showed higher umami values than the control and other treatment groups. Therefore, it is expected that GD can be valuable as a starter culture unique to Korea when manufacturing fermented sausage.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.660-665
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• 2003

High-level expression of Thermus caldophilus GK24 alkaline phosphatase (Tca APase) was achieved in Escherichia coli using the pET-based expression plasmids, pEAP1 and pEAP2. In the case of plasmid pEAP2, the signal peptide region of Tca APase was replaced by the PelB leader peptide of expression vector pET-22b(+). Furthermore, the expression level was somewhat higher than that of plasmid pEAPl. A rapid purification procedure of Tca APase overproduced in E. coli was developed which involved heating to denature E. coli proteins followed by HiTrap Heparin HP column chromatography. Optimal temperature and pH and $Mg^{2+}$ dependence of the recombinant Tca APase were similar to those of native enzyme isolated from T. caldophilus GK24.

• Restorative Dentistry and Endodontics
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• 제15권1호
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• pp.129-139
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• 1990

In order to determine if GK-101E(N-monochloro-DL-2-aminobutyrate) solution was effective in the removal of carious dentin, this study was conducted on 75 extracted human carious teeth. GK-101E solution was compared in effectiveness to saline solution.75 extracted human carious teeth were randomly assigned to two groups accordingly : (1) 50 teeth were treated with GK-101E solution : and (2) 25 teeth were treated with saline solution. Teeth in each group were divided into the medium or medium-hard consistency. Treatment consisted of directing the pulsating stream of each coded test solution utilizing the Caridex$^{TM}$ Caries Removal System against the carious surface for 4 mimutes. One operator treated all teeth with corded solution, and four investigators scored on the treated teeth based on visual examination and tactile evaluation with a dental explorer in conjunction with staining method with Caries Detector$^{TM}$ The results were as follows : 1. All groups showed various effect of carious dentin removal. 2. GK-101E solution was statistically superior to physiologic saline solution in the removal of carious dentin, regardless of carious consistency. 3. GK-101E solution in the medium-hard consistency was statistically superior to that in the medium consistency in the caries removal(p=0.001). 4. Saline solution in the medium consistency was equivalent to that in the medium-hard consistency in caries removal(p>0.1).

• 항공우주기술
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• 제13권2호
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• pp.115-121
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• 2014

천리안위성(2010년 발사)의 성공적인 개발을 바탕으로 두 기의 위성을 동시에 개발하는 정지궤도 복합위성(GEO-KOMPSAT-2) 프로그램이 진행 중이다. 정지궤도 복합위성 중 GEO-KOMPSAT-2A(GK2A)위성에는 차세대 고성능 기상 탑재체와 우주기상 탑재체가 개발 탑재될 예정이다. 국내 주도로 개발되는 우주기상 탑재체는 대한민국 최초의 정지궤도용 우주기상 센서가 될 예정이다. 세 가지 종류의 우주기상 탑재체(고에너지 입자 검출기, 자력계, 위성 대전 감시기)는 정지궤도에서 우주공간에 대한 물리적 현상(고에너지 입자 분포, 지구 주위의 자기장, 위성 대전 전류)을 관측하여 우주기상 예 경보에 활용될 목적을 가지고 있다. 본 논문에서는 GK2A위성의 부탑재체인 우주기상탑재체 개발 요구사항, 시스템 설계 및 접속 설계에 대해 요약 기술하였다.

• 혜화의학회지
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• 제24권2호
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• pp.35-57
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• 2016

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, $100{\mu}g/ml$). 4. NO, IL-6, TNF-${\alpha}$, MCP-1 production were significantly decreased in GK extract(at 10, $100{\mu}g/ml$). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, $100{\mu}g/ml$). IL-$1{\beta}$, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

• 대한원격탐사학회지
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• 제37권1호
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• pp.41-55
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• 2021

To detect thunderstorms occurring in Korea, National Meteorological Satellite Center (NMSC) also introduced the rapid-development thunderstorm (RDT) algorithm developed by EUMETSAT. At NMCS, the H-RDT (HR) based on the Himawari-8 satellite and the K-RDT (KR) which combines the GK2A convection initiation output with the RDT were developed. In this study, we optimized the KR (KU) to improve the detection level of thunderstorms occurring in Korea. For this, we used all available data, such as GK2A/AMI, RADAR, lightning, and numerical model data from the recent two years (2019-2020). The machine learning of logistic regression and stepwise variable selection was used to optimize the KU algorithms. For considering the developing stages and duration time of thunderstorms, and data availability of GK2A/AMI, a total of 72 types of detection algorithms were developed. The level of detection of the KR, HR, and KU was evaluated qualitatively and quantitatively using lightning and RADAR data. Visual inspection using the lightning and RADAR data showed that all three algorithms detect thunderstorms that occurred in Korea well. However, the level of detection differs according to the lightning frequency and day/night, and the higher the frequency of lightning, the higher the detection level is. And the level of detection is generally higher at night than day. The quantitative verification of KU using lightning (RADAR) data showed that POD and FAR are 0.70 (0.34) and 0.57 (0.04), respectively. The verification results showed that the detection level of KU is slightly better than that of KR and HR.

• 대한원격탐사학회지
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• 제37권5_1호
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• pp.1029-1042
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• 2021

차세대 중형위성 사업의 일환으로 농지 및 산림에서의 원격 탐사를 위하여 농림위성 (차세대 중형위성 4호)이 발사 예정에 있다. 위성 영상에서 식생의 정량적인 정보를 얻기 위해서는 대기보정을 통한 지표 반사도 취득이 선행되어야 하므로 농림위성을 위한 대기보정 기술 개발은 불가피할 것으로 생각된다. 특히 대기에서의 흡수와 산란 특성은 파장에 따라 다르게 나타나므로 농림위성 파장 영역을 고려한 대기보정 파라미터 민감도 분석이 필요하다. 또한, 농림위성은 5개 채널(Blue, Green, Red, Red edge, Near-infrared)을 보유하고있어 대기보정 주요 파라미터인 AOD (Aerosol optical depth)와 WV (Water vapor)를 직접 산출하기 어려우므로 이를 외부에서 제공할 수 있는 방안을 마련할 필요가 있다. 따라서, 본 연구에서는 농림위성과 유사한 사양을 가진 Sentinel-2 위성 영상을 이용하여 주요 파라미터인 AOD, WV, O₃ 민감도 분석을 수행하고, 파라미터 제공을 위해 천리안 2A (GK2A; GEO-KOMPSAT-2A) 정지궤도 복합위성의 산출물을 이용하여 대기보정 파라미터로서의 활용 가능성을 살펴보았다. 민감도 분석 결과는 AOD가 가장 중요한 파라미터임을 보여주었으며, 근적외선 채널보다는 가시광 채널에서 더 큰 민감도를 가지는 것으로 나타났다. 특히 Blue 채널에서 AOD의 20%의 변화는 지표 반사도에서 약 100%의 오차율을 야기하므로 정확한 지표 반사도 취득을 위해서는 높은 신뢰성을 가진 AOD가 필요할 것으로 생각된다. GK2A AOD 산출물을 이용한 대기보정 결과는 토지피복별 분류 가능성을 이용하여 Sentienl-2 L2A 자료와 비교한 결과, 두 모델별 분류 가능성은 유사하였으나, 파장대가 짧은 영역일수록 GK2A AOD 산출물을 적용한 대기보정 결과가 Sentinel-2 L2A보다 높게 나타났다. 이를 통해 GK2A에서 제공되는 산출물이 향후 농림위성 대기보정 파라미터로서 충분히 활용될 수 있을 것으로 판단된다. 본 연구의 결과는 추후 농림위성 발사 후 대기보정에 참고 자료로서 활용될 수 있을 것으로 기대된다.

• 한국식품영양과학회지
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• 제46권5호
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• pp.552-561
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• 2017

본 연구에서는 가시오갈피 물 추출물(ASW)를 이용하여 아직 시도된 바가 없는 HepG2 세포 내 포도당 유입과정 및 glucokinase(GK) 활성을 통한 포도당 이용대사 실험을 수행하였다. 포도당의 세포 내 유입은 GLUT2의 transcription factor들 중 하나인 $HNF-1{\alpha}$의 활성화로 GLUT2의 유전자 발현이 증가하여 이루어지는 것을 확인하였다. GK 활성 측정 결과 ASW가 GK를 활성화하여 포도당의 인산화에 영향을 주는 것을 확인하였고 AMP-activated protein kinase의 인산화 증가로 glycolysis에 관여하는 효소인 GK의 단백질 발현은 증가하고, gluconeogenesis에 관여하는 phosphoenolpyruvate carboxykinase의 단백질 발현은 감소하는 것을 확인하였다. 그리고 인산화된 포도당이 glycogen으로 전환 저장되는 메커니즘을 pPI3k-pAkt-pGSK-$3{\beta}$의 단계별 단백질 발현을 확인함으로써 검증하였으며, glycogen 함량 측정을 통해 확인하였다. 본 연구를 통해 ASW가 다양한 메커니즘에 작용하여 당뇨의 예방 및 개선에 활용할 수 있는 잠재적 소재임을 확인하였고, 이는 ASW가 천연 기능성 소재로서의 개발가치가 높음을 시사한다.