• 한국우주과학회:학술대회논문집(한국우주과학회보)
• /
• 한국우주과학회 1992년도 한국우주과학회보 제2권1호
• /
• pp.16-16
• /
• 1993

The Cosmic Mach number M is the ratio of the bulk flow velocity of the galaxrvelocity field on some scale R to the unall scale velocity dispersion within refcions of scale R. Because M is the ratio of two velocities, it is inn-dimansionat and the Here, independent of the amplitude of the power specHim and of the biasplnmeter in the linear theory. We have measured the Mach rnlmber for two observational samples: a spiral galaxy sample(AHM) of Aaronson and hiscoBlaborators with absolute distances measured by the infrared Ttillr-Fisher relatioa and an elliptical galaxy sample(EGALS) of Faber or 0, with distances determined by the relation. The effective depths distances of galaxies from the Local Group of these samples are 1639 km/s and 2862 e/s, respectivelr. The Machnumbers from these observed peculiar velocity Selds He fund as M=0.95 for AHMand M=0.59 for EGALS. We comPBre these calculated Mach numbers with thosefrom meck surweys drawn fuom three cosnulogical medels: the stand8rd biased nh=0.5 CDM modet an open CDM rrudel with gh=0.2, and a medd with thepower-law power specelm P(k)-k-1 and n=1. The Mach rnlmber test can give robust constraints on these cosmelogical nudels whose power spectra have very different shapes at large scales.

• 미생물학회지
• /
• 제42권4호
• /
• pp.313-318
• /
• 2006

가정에서 제조된 된장으로부터 cellulase 생산균으로 분리된 고온성 WL-12는 형태적 특성, 생화학적 성질 및 16S rRNA의 염기서열에 근거하여 Bacillus licheniformis로 동정되었다. B. licheniformis WL-12의 cellulase 유전자를 클로닝하여 그 염기서열을 결정한 결과 cellulase 유전자(celA)는 517 아미노산으로 구성된 단백질을 코드하며 1,551 뉴클레오티드로 이루어졌다. 아미노산 잔기배열을 분석한 결과 WL-12의 cellulase는 활성영역과 cellulose 결합영역으로 구성되어 있었으며, glycosyl hydrolase (GH) family 5에 속하는 B. licheniformis, B. subtilis와 B. amyloliquefaciens의 cellulase와 높은 상동성을 보였다. 클론된 celA를 발현용 vector에 도입하여 B. subtilis에서 발현시켜 cellulase 최대생산성이 7.0 units/ml에 이르렀다.

• Preventive Nutrition and Food Science
• /
• 제16권2호
• /
• pp.110-116
• /
• 2011

This study examined the growth effects of yeast hydrolysate (YH) and a traditional Korean herbal mixture (HM, a mixture of safflower seed and gasiogapi extract). Three-week old male SD rats were divided into the following five groups: negative control (saline), positive control (foremilk 0.5 g/kg/day), YH (YH 0.5 g/kg/day), HM (HM 0.2 g/kg/day), and YH+HM (YH 0.5 g/kg/day and HM 0.2 g/kg/day). Tibia bone length was 9.22 mm in the normal control rats, while both the YH and YH+HM groups had significantly longer tibia bones than the control rats (9.75 mm and 10.46 mm, respectively). The proximal epiphyses of YH, HM, and YH+HM measured 0.75, 0.70, and 0.75 mm, respectively, while the length in the control group was 0.50 mm. Plasma insulin growth factor-1 (IGF-1) level was slightly higher in the YH group (1.36 mg/mL) than in the control rats (1.29 mg/mL), but the difference was not significant. Plasma IGF-1 level was significantly increased in the HM (1.49 mg/mL) and YH+HM (1.53 mg/mL) groups compared to the control group (1.29 mg/mL). Growth hormone (GH) levels in YH (17.45 ng/mL), HM (15.49 ng/mL), and YH+HM (16.07 ng/mL) were significantly different compared to the control group (3.63 ng/mL).

• Clinical and Experimental Pediatrics
• /
• 제52권2호
• /
• pp.227-233
• /
• 2009

목 적 : 소아청소년기 GHD 환자들은 성인기에 진단된 환자보다 체성분과 심혈관계의 변화가 심한 것으로 알려져 있어 성인기에도 GHD가 지속되면 성장호르몬 치료를 꼭 받아야 한다. 본 연구에서는 소아청소년기에 GHD로 진단 받은 성인 환자들을 대상으로 ITT를 시행하여 성인기에도 GHD가 지속되는 요인을 살펴보고, 성인기에도 GHD가 지속될 가능성이 높은 대상을 알아보고자 한다. 방 법 : 성인키에 도달한 55명(남 39명, 여 16명)을 대상으로 ITT의 최대 성장호르몬 값이 $3{\mu}g/L$ 미만이면 성인기 GHD로 진단하였다. 특발성 GHD 환자(n=12)는 뇌하수체 구조적인 이상이 있는 2명과 둔위 분만 또는 가사의 병력이 있는 10명이었으며, 기질성 GHD 환자(n=43)는 시상하부-뇌하수체를 침범하는 뇌종양(n=33), 그 외의 뇌종양(n=3), 뇌수막염(n=3), 백혈병(n= 2), 지주막낭종(n=1), 뇌하수체염(n=1)에 해당하였다. 결 과 : 성인기에도 GHD가 지속된 환자는 49명(89.1%)이었다. 최대 성장호르몬 값의 log값은 기저 IGF-I 농도와 양의 상관관계를 보였다(r=0.57, P<0.001). 특발성 GHD 환자의 91.7%가 성인기에도 GHD가 지속되어, 성인기 GHD 여부는 소아청소년기 특발성, 기질성 GHD 여부에 따른 차이가 없었다. 동반된 뇌하수체호르몬 결핍 개수가 많을수록(단독 GHD 40%, 1개의 결핍 동반 80%, 2개 이상 동반 95.6%, P=0.002), 기질성 GHD 환자 중에서 병변이 시상하부-뇌하수체를 일차적으로 침범하는 경우(P=0.003)에 성인기에도 GHD가 지속될 가능성이 높았다. 두개 내 방사선 치료를 받은 18명 중에서 15명이 성인기에도 GHD가 지속되었는데, 시상하부-뇌하수체를 침범하는 종양으로 방사선 치료를 받은 환자들 14명은 모두 방사선조사량에 관계없이 성인기에도 GHD가 지속되었다. 동반된 뇌하수체호르몬 결핍이 있는 경우(P=0.005), 소아청소년기 완전 GHD인 경우(P=0.005)에 성인기에도 GHD가 지속되는 비율이 높았다. 결 론 : 소아청소년기에 GHD로 진단 받은 환자들 중에서 동반 뇌하수체호르몬 결핍이 2개 이상이거나, 기저 질환의 병변이 시상하부-뇌하수체를 포함하면 성인기에도 GHD가 지속될 가능성이 높다.

• 한국산림과학회지
• /
• 제76권3호
• /
• pp.181-192
• /
• 1987

각산정(角算定) 측정법(測定法)으로 임분재적(林分材積)을 추정(推定)할 수 있도록 하기 위하여 경기, 충남북, 강원지역 등의 소나무임분(林分)에서 표준지(標準地) 164개소(個所)를 선정(選定)하여 각(各) 조사인자별(調査因子別)로 실측(實測)하고 Plotless Sampling 방법(方法)으로 측정(測定)한 자료(資料)를 가지고 임분재적(林分材積) 조제방법(調製方法)과 Plotless Sampling 법(法)에 필요한 각종(各種) 표(表)를 조제하여 이를 종합적으로 분석검토(分析檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 각산정(角算定) 측정법(測定法)에 의하여 측정(測定)이 가능(可能)한 임분평균수고(林分平均樹高), ha 당(當) 단면적(斷面積), 단면적수고(斷面積樹高)의 측정치(測定値)와 표준지(標準地)에서 측정(測定)한 실측치(實測値) 사이에는 회귀계수(回歸係數) b 가 거의 1인 Y=bx 의 회귀계수(回歸係數)가 성립(成立) 하였으며 이들 사이에는 유의차(有意差)가 없어 소나무림(林)에서 각산정(角算定) 측정법(測定法)로 임분재적(林分材積)을 추정(推定)할 수 있다. 2. Dendrometer 로 측정(測定)이 가능한 인자(因子)를 이용(利用)하여 구한, 가장 적합(適合)한 임분재적식(林分材積式)은 log V = -0.0208+0.8497 log G. H., log V = -0.0028+0.7981 log G+0.9313 log H 이며 본식(本式)에 의하여 조제된 임분재적표(林分材積表)는 Table 4, 5와 같으며 표(表)에 의한 측정치(測定値)와 실측치간(實測値間)의 추정오차율(推定誤差率)은 1변수재적표(變數材積表)가 9.16%, 2변수재적표(變數材積表)가 8.50%이었다. 3. 각산정(角算定) 측정법(測定法)에 의한 임분재적(林分材積) 추정시(堆定時) 필요(必要)한 인자(因子)인 임분형상고(林分形狀高), 형상단면적(形狀斷面積), 임분형수(林分形數) 등을 구할 수 있는 가장 적합(適合)한 추정식(推定式)은 다음과 같다. FH=D/(1.5205+0.0994 D) log FH=0.0451+0.2429 log D+0.3474 log H log FG=-0.0380+0.7758 log G-0.0066 log H F=H/(-5.1697+2.6013 H) F=FH/(-3.1256+2.7611 FH) log F=-0.0634-0.0848 log GH-0.1224 log Di 4. 이와 같은 식(式)에 의하여 조제한 임분형상고표(林分形狀高表)는 Table 7, 8과 같으며 이의 추정오차율(推定誤差率)은 임분평균직경(林分平均直徑)에 의한 1변수표(變數表)와 임분평균직경(林分平均直徑) 및 임분평균수고(林分平均樹高)에 의한 2변수표(變數表)는 각각(各各) 8.05%, 8.32%이며 단면적(斷面積)과 임분평균수고(林分平均樹高)의 2변수(變數)에 의한 형상단면적표(形狀斷面積表)는 Table 9와 같고 이의 추정오차율(推定誤差率)은 9.76%이었다. 또한 임분형수표(林分形數表)는 Table 10, 11, 과 같으며 이의 추정오차율(推定誤差率)은 임분평균수고(林分平均樹高), 임분형상고(林分形狀高)에 의한 1변수표(變數表)는 각각(各各) 5.58%, 5.39%이고 단면적(斷面績) 수고(樹高)와 수간거리(樹幹距離)에 의한 2변수표(變數表)는 4.30% 이었다.

• 생명과학회지
• /
• 제25권6호
• /
• pp.680-685
• /
• 2015

이전에 토양으로부터 cellulase와 xylanase 생산 균주로 단리하였다. 단리한 균주 유래의 16S rRNA 유전자 및 API 50 kit를 분석한 결과 Bacillus subtilis와 약 99.5%의 높은 상동성을 보였기에 본 균주를 B. subtilis NC1으로 명명하였다. Bacillus subtilis NC1 균주 유래 cellulase와 xylanase 유전자를 cloning 하여 유전자 배열을 규명하였다. 또한, 두 효소의 아미노산 배열을 이용하여 상동성을 검토한 결과 cellulase는 Glycoside hydrolase family (GH) 5 그리고 xylanase는 GH30에 속하는 효소임을 밝혔다. 본 연구에서는 B. subtilis NC1 의 cellulase 생산을 위한 배지성분의 최적 농도를 결정하기 위해 중심합성계획법(central composite design, CCD)을 기반으로 한 반응표면 분석법(Response Surface Methodology) 을 수행하였다. 세가지 독립변수로는 tryptone, yeast extract, 그리고 NaCl이 조사되었다. 반응값에 대하여 분산분석을 실시한 결과 결정계수(R²)는 0.96이었으며 전체 모델에 대한 유의확률이 0.0001로 매우 높은 유의성을 지님을 확인하였다. 반응표면분석법을 통하여 얻어진 B. subtilis NC1의 cellulase 활성을 위한 최적화 배지의 각 변수 농도는 tryptone 2.5%, yeast extract 0.5%, 그리고 NaCl 1.0%로 예측 되었다. 최적화 배지에서의 B. subtilis NC1의 cellulase 활성을 검증한 최적화를 실시하기 이전인 대조구의 cellulase 활성 0.5U/ml와 비교하면 24% 활성이 향상된 0.62U/ml의 높은 활성을 보였다.

• Toxicological Research
• /
• 제16권2호
• /
• pp.95-100
• /
• 2000

In previous study, both constitutive expression and 3-methylcholanthrene (3MC)-mediated elevation of CYP1A2 mRNA were demonstrated in human hepatoma HepG2 cells by reverse transcription-polymerase chain reaction (RT-PCR), suggesting that HepG2 cells would be appropriate for the study of human CYP1A2 regulation(Chung and Bresnick, 1994). Further studies were conducted to determine the basis of this induction phenomenon that is observed in HepG2 cells. Since CYP1A1 gene, another polycyclic hydrocarbon(PH)-inducible gene, is regulated by PHs through their interactions via receptors with cis-elements, the 5'-flanking region of human CYP 1A2 gene was analyzed to search such responsive elements. The promoter activity of various lengths of CYP1A2 gene sequence (-3203/+58bp) was measured in transiently-transfected HepG2 cells by fusion constructs containing the CAT, hGH or luciferase genes as a reporter. This region of the CYP1A2 gene, although containing a XRE, was only weakly responsive (less than 2 fold induction) to 10 nM of TCDD or 1 $\mu$M 3 MC treatment. This small enhancement of promoter activity is inconsistent with the previous observation, i.e., 12 to 14 fold-enhanced CYP1A2 mRNA from 1 $\mu$M 3 MC treated HepG2 cells, suggesting that additional mechanisms would exist for PH-mediated induction of CYP1A2 in these cells.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권10호
• /
• pp.1397-1401
• /
• 2003

The aim of this study was to determine genotypes of four genetic markers and to investigate their association with milk production traits in Brown Swiss cattle imported to Slovakia. The bovine $\kappa$-casein, $\beta$-lactoglobulin, growth hormone and prolactin genotypes of 107 cows were identified by polymerase chain reaction. Effects all four genetic markers on milk, fat, protein and lactose yields and fat, protein and lactose percentage were estimated from a data set of 249 lactations. The frequency of desirable B allele of $\kappa$-casein gene to milk production was 0.46, alleles A of $\beta$-lactoglobulin gene was 0.55, allele and L of growth hormone gene was 0.45 and allele A and B of bovine prolactin gene were 0.61 and 0.39. The results of milk production obtained in our work showed that BB genotypes of $\kappa$-CN gene, AA genotypes of $\beta$-LG gene, LL genotypes of bGH gene were significantly associated with better milk production traits, mainly about the fat content. Association of a bovine prolactin genotypes with milk production were not found.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권1호
• /
• pp.1-5
• /
• 2008

To analyze genetic effects on milk production traits, 164 unrelated Italian Jersey cows reared in 15 farms located in Southern Italy were characterized at the growth hormone locus. Using a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism protocol all three possible genotypes for the Leucine/Valine polymorphism were identified even if in the considered population the number of homozygous animals (Leucine/Leucine = 36; Valine/Valine = 28) was smaller than Leucine/Valine (n = 100) cows; the Hardy-Weinberg equilibrium was verified. Statistical analysis showed that daily milk yield in the Leucine/Leucine genotype was higher (p<0.01) than in Leucine/Valine (22.76 kg vs. 16.96 kg ); cows with Leucine/Valine genotype differed significantly from Leucine/Leucine and Valine/Valine genotypes particularly in fat (4.95% vs. 4.13% and 4.82%, respectively) and protein content (4.00% vs. 3.47% and 3.79%, respectively). No significant effect on lactation length was observed.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권6호
• /
• pp.750-756
• /
• 2010

Two trials were conducted to study the effects of two Chinese herbal polysaccharides, Astragalus polysaccharides (APS) and Achyranthes bidentata polysaccharides (ABPS), and one Chinese herbal saponin, Acantbepanax senticosus saponin (ASS), on the immunity and growth performance of weaned pigs. Experiment 1 was a 14-day growth assay, in which 32 weaned pigs were randomly allocated to one of four dietary treatments: i) 0.05% talcum powder control; ii) 0.05% APS; iii) 0.05% mixture of APS and ASS in a 1:1 ratio by weight; and iv) 0.05% mixture of APS, ASS, and ABPS in a ratio of 1:1:1 by weight. Blood samples were collected on day 14 to determine plasma parameters. Feed intake, body weight gain, and feed efficiency were also determined. Experiment 2 was a 21-day immunity assay, in which 16 weaned pigs were randomly allotted to one of two dietary treatments: i) 0.05% talcum powder control; and ii) 0.05% mixture of APS and ASS in a 1:1 ratio by weight. On day 21, pigs were challenged with lipopolysaccharide (LPS) and 3 h later blood samples were collected and analyzed for lymphocyte proliferation as well as interleukin 6 (IL-6), insulin-like growth factor 1 (IGF-1), growth hormone (GH), and cortisol levels. In Experiment 1, feeding Chinese herbal polysaccharides and saponin increased growth performance of the pigs. The effects of the mixture of APS and ASS were especially notable, as there was a significant improvement in growth performance compared with the control (p<0.05). The plasma concentration of immunoglobulin G (IgG), nitric oxide (NO), and nitric oxide synthase (NOS) were increased in all treatments groups, with the mixture of APS and ASS increasing the level of IgG and NOS significantly (p<0.05), compared with the control. There was no difference in the NO level between the control and treatment groups (p>0.05). In Experiment 2, Chinese herbal polysaccharides and saponin showed immunostimulating effects. The level of cortisol, GH, and IGF-I were significantly increased (p>0.05), and the level of IL-6 showed a significant decrease (p<0.05) in the APS and ASS treatment after the LPS challenge. The mixture of APS and ASS could stimulate the blood lymphocyte proliferation significantly whether the LPS was injected or not (p<0.05). These results show that Chinese herbal extracts can improve growth performance and stimulate immunity of weaned pigs. A mixture of APS and ASS, compared with APS alone, could be a new kind of immunostimulant for weaned pigs, which could result in greater positive effects on their growth performance and immunity.