• Journal of the Korean earth science society
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• v.32 no.7
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• pp.732-749
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• 2011

In this study, 27-year (1979-2005) regional climate over the CORDEX East Asia domain was reproduced using a regional climate model, RegCM4, driven by HadGEM2-AO output, and the model's simulation skill was evaluated in terms of surface air temperature and precipitation. The RegCM4 reasonably simulated the spatial distribution and interannual variability and seasonal variability of surface air temperature, while it had systematic biases in the simulation of precipitation. In particular, simulated rainband of East Asian summer monsoon was southward shifted below $30^{\circ}N$ as compared with the observation, thereby, summer mean precipitation over South Korea was significantly underestimated. Simulated temperature from the RegCM4 driven by the HadGEM2-AO output was comparable to that driven by the reanalysis. However, the RegCM4 driven by the HadGEM2-AO had prominently poor skill in the simulation of precipitation. This can be associated with the distorted monsoon circulations in the driving data (i.e., HadGEM2-AO) such as southward shifted low-level southwesterly, which resulted in the erroneous evolution of East Asian summer monsoon simulated by RegCM4.

• Biomolecules & Therapeutics
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• v.29 no.2
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• pp.184-194
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• 2021

Histone acetylation is a well-characterized epigenetic modification controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Imbalanced histone acetylation has been observed in many primary cancers. Therefore, efforts have been made to find drugs or small molecules such as HDAC inhibitors that can revert acetylation levels to normal in cancer cells. We observed dose-dependent reduction in the endogenous and exogenous protein expression levels of KAT8 (also known as human MOF), a member of the MYST family of HATs, and its corresponding histone acetylation at H4K5, H4K8, and H4K16 in chemotherapy drug gemcitabine (GEM)-exposed T24 bladder cancer (BLCA) cells. Interestingly, the reduction in MOF and histone H4 acetylation was inversely proportional to GEM-induced γH2AX, an indicator of chemotherapy drug effectiveness. Furthermore, pGL4-MOF-Luc reporter activities were significantly inhibited by GEM, thereby suggesting that GEM utilizes an MOF-mediated anti-BLCA mechanism of action. In the CCK-8, wound healing assays and Transwell® experiments, the additive effects on cell proliferation and migration were observed in the presence of exogenous MOF and GEM. In addition, the promoted cell sensitivity to GEM by exogenous MOF in BLCA cells was confirmed using an Annexin V-FITC/PI assay. Taken together, our results provide the theoretical basis for elucidating the anti-BLCA mechanism of GEM.

• Proceedings of the Korean Nuclear Society Conference
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• 2003.10a
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• pp.416.1-416.1
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• 2003

• Proceedings of the Korean Nuclear Society Conference
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• 1999.10a
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• pp.334-334
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• 1999

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.88-95
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• 1992

The genetically engineered microorganisms (GEMS) could be released accidentally or ii)rexperimental purposes, as the genetic engineering, technique ha:. become very popular inany laboratories of biological sciences. But there have been littlt: informations on transkrbehavior of the genetically ~nodified genes in the natural en\ironmentx. In this stutly.antibiotic resistant bacteria were isolated from nat.ural waters. and then GEM strains wereconstructed i'rom the natural isolate (NI) by ~noclification oi' the Km' plasmitl. Thetransferability of the plasmids in the GEM and NI strains were examinetl by con-jugationin Luria-Bertani broth :it 30$^{\circ}$C. Also the cff'ccts 01' mating strain and pH on their transferfrequency and rearrangement of the plasmids in tl-~ec o~ijugantsM ere comp:irati\ely stuclictl.I'hc transkr frequency of Km' plasmid in donor of GEM and N1 strains wah similar a.;about 10 ' when co~ljugation was conducted wit11 M'I'I strain is recipient at pH 7. butthat of 1)KCOOI was lowered to 1.2X 10 '. And when the lab. stlain was uhccl as recipient.the transfer tendency of the plasmid was about same in both (;EM and NI strains usedas donor. All thc tionor 5trains. except for I)KC601. showecl the Ilighcs~ frequency of about10 ' at pH 7 and the frequcncics were lowered at both pH 5 and 9. Hut the mocliliedKm' plasmid in the cloned strain of DKC601 was transferred hy very low frequency of10 "at pH 5 ant1 7 comparing to other GEM strains. especiall! any co~~.jugantws ere notobtained at pH 4 and 9 even after conjugation for 6 hours. Rearrangement of the plasmidstranskrred into the lab. strain was not found in the conjugants. I\ulcornerut a lot of rearrangclncntwas ohservecl nlhen they were transferred into the NI strain. Such a rearrangement wasmore severe when donor was GEM strain rather then NI strain Hut such ;r phenomenonwas less affected by p!-l values.r phenomenon was less affected by p!-l values.

• BMB Reports
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• v.43 no.2
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• pp.79-90
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• 2010

Mouse models are crucial for the functional annotation of human genome. Gene modification techniques including gene targeting and gene trap in mouse have provided powerful tools in the form of genetically engineered mice (GEM) for understanding the molecular pathogenesis of human diseases. Several international consortium and programs are under way to deliver mutations in every gene in mouse genome. The information from studying these GEM can be shared through international collaboration. However, there are many limitations in utility because not all human genes are knocked out in mouse and they are not yet phenotypically characterized by standardized ways which is required for sharing and evaluating data from GEM. The recent improvement in mouse genetics has now moved the bottleneck in mouse functional genomics from the production of GEM to the systematic mouse phenotype analysis of GEM. Enhanced, reproducible and comprehensive mouse phenotype analysis has thus emerged as a prerequisite for effectively engaging the phenotyping bottleneck. In this review, current information on systematic mouse phenotype analysis and an issue-oriented perspective will be provided.

• Journal of Environmental Health Sciences
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• v.34 no.6
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• pp.414-422
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• 2008

Methane is a potent greenhouse gas and methane emissions from landfill sites have been linked to global warming. In this study, LandGEM (Landfill Gas Emission Model) was applied to predict landfill gas quantity over time, and then this result was compared with the data surveyed on the site, Cheongju Megalo Landfill. LandGEM allows the input of site-specific values for methane generation rate (k) and potential methane generation capacity $L_o$, but in this study, k value of 0.04/yr and $L_o$ value of $100\;m^3$/ton were considered to be most appropriate for reflecting non-arid temperate region conventional landfilling like Cheongju Megalo Landfill. Relatively high discrepancies between the surveyed data and the predicted data about landfill gas seems to be derived from insufficient compaction of daily soil-cover, inefficient recovery of landfill gas and banning of direct landfilling of food waste in 2005. This study can be used for dissemination of information and increasing awareness about the benefits of recovering and utilizing LFG (landfill gas) and mitigating greenhouse gas emissions.

• Journal of The Korean Astronomical Society
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• v.41 no.3
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• pp.59-64
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• 2008

To detect exoplanets and study pulsation of K giant stars, we have observed precise RV (radial velocity) of about 55 early K giant (K0 - K4) stars brighter than V = 5 magnitude since 2003 by using BOES, a high resolution Echelle spectrograph attached to the 1.8 m telescope at BOAO (Bohyunsan Optical Astronomy Observatory). We detected periodic RV variation of KO III star $\beta$ Gem (HD 62509) with a period $P\;=\;596.6\;{\pm}\;2.3$ days and a semi-amplitude $K\;=\;44.8\;{\pm}\;0.7\;ms^{-1}$. If we adopt 1.7 $M_{\odot}$ for the mass of $\beta$ Gem, this yields the minimum mass of the companion m sin i = 2.64 $M_{Jupiter}$. Our results agree well with Hatzes et al. (2006) and Reffert et al. (2006), and confirm their discovery of a planetary object around $\beta$ Gem. We also confirmed about 192 minutes short period stellar oscillation found by Hatzes and Zechmeister (2007). This is the first report of exoplanet detection using BOES and demonstrates that the RV observation using BOES is accurate and stable enough to detect exoplanets around bright K giant stars.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.343-343
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• 2012

미래 극한사상의 초과확률을 산정하기 위하여 저해상도의 전지구 기후변화 시나리오 자료를 그대로 사용하거나 이를 역학적 또는 통계적 방법으로 상세화한 고해상도 기후변화 시나리오 자료를 활용한다. 통계적 상세화는 전지구 또는 지역기후모델의 현재기후 모의 자료와 관측 자료와의 통계적 관계를 미래 예측자료에 적용하는 방법으로, 현재와 미래 기후의 시공간적 분포가 동일하다는 가정을 포함하고 있다. 반면 역학적 상세화 방법은 기후변화 강제력을 고려하는 지역기후모델을 이용하여 기후시스템의 역학 및 물리과정, 기후시스템간 의 상호작용, 기후변화의 비정상성 등을 고려할 수 있고, 변수간의 시공간적 상관성을 지구시스템의 물리 역학적 과정으로 해석할 수 있다는 장점이 있다. 이에 국립기상연구소에서는 영국 기상청의 통합모델(UM)기반의 지역기후모델(HadGEM3)을 사용하여 50 km 및 12.5 km 격자 단위로 역학적 상세화(dynamic downscaling)를 수행하였다. 본 연구에서는 역학적 상세화로 생산된 HadGEM3-RA 자료를 이용하여 현재기후(1980-2005), 가까운 미래(2020-2049)와 21세기말(2070-2099)의 20년 빈도 강수량을 비교하였다. 연구결과, 남한에 걸쳐 현재기후에 비하여 미래에는 극한강수의 크기와 빈도가 전반적으로 증가하는 경향을 확인할 수 있었다. 20년에 한번씩 발생하였던 일 극한강수는 RCP8.5를 고려한 21세기말에는 약 4년에 한번씩 발생하리라 전망되었다.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.32-32
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• 2017

기후변화에 따른 이상가뭄과 홍수의 영향으로 하천의 수문과 수리적 요소가 변화되고 그로인해 발생하는 지형변화와 그에 따른 수질변화 분석이 필요하다. 낙동강의 효율적인 수질관리를 위해서는 수질과 더불어 유역관리도 함께 이루어져야 한다. 기후변화로 인한 가뭄이나 홍수 등 극한 사상이 보 같은 인공구조물이 설치되어 정체성 수역이 된 낙동강 수계에 어떠한 수질변화가 일어나는지에 대한 연구가 필요하며 이를 고려한 체계적이고 통합적인 수질관리대책이 필요하다. 본 연구에서는 낙동강 유역의 합천창녕보와 창녕함안보 구간에서 HadGEM3-RA RCP 4.5와 8.5를 적용하여 미래기후시나리오를 예측하여 수문분석을 수행하였다. 수문분석 결과인 유출량을 하상변동모형의 입력자료로 적용하여 상?하류 구간의 지형변화에 미치는 영향을 분석하고자 하였다. 마지막으로 지형변화가 수질에 미치는 영향을 분석하고자 QUAL-MEV 수질모형을 적용하여 지형변화에 따른 수질분석의 상대적 비교를 통해 기후변화와 지형변화의 영향을 분석하고자 하였다. 연구결과, 기후시나리오의 경우 HadGEM3-RA RCP 4.5 보다 8.5에서 강수가 6% 증가하는 결과를 나타내고 그에 따라 유출량도 증가하고 있다. 하상변동 분석결과, 유량이 많은 풍수년에 침식과 퇴적양상이 크게 나타나고 RCP 8.5에서 변동 폭이 크게 나타났다. 하천지형변화를 고려한 수질분석결과, 유량의 증가에 따라 수질도 개선되는 결과를 나타내고 있다.