• Parasites, Hosts and Diseases
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• v.39 no.4
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• pp.313-318
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• 2001

The identification , characterization and quantification of Plasmodium sp. genetic polymorphism are becoming increasingly important in the vaccine development. We investigated polymorphism of Plasmodium vivax GAM-1 (PvGAM-1) gene in 30 Korean isolates. The polymorphic region of the PvGAM-1 gene, corresponding to nt 3792-4029, was amplified using polymerase chain reaction (PCR) followed by sequencing. All of the P. viuax Korean isolates were one type of GAM-1 gene, which were identical to that of the Belem strain. It is suggested that PvGAM-1 could not be used as a genetic marker for identifying or classifying P. vivax Korean isolates. It revealed that the polymorphic pattern as acquired basically by duplication and modification or deletion event of a 33 bp-motif fragment ended by poly guanine (G) and that there were at least three complete and one partial 33 Up-motif sequences within the polymorphic region in the longest cases such as those of South Korean and Belem isolates. In addition, we clustered P. vivax isolates with parsimonious criteria on the basis of PvGAM- 1 polymorphic patterns (insertion/deletion patterns) .

• Herbal Formula Science
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• v.15 no.1
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• pp.37-47
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• 2007

許浚(Heo Jun), the best physician on his day, wrote 東醫寶鑑(DongEuiBoGam, 1613) based on his clinical experience as a court doctor and life-long study of over 500 medical books. Now most clinicians consult 東醫寶鑑(DongEuiBoGam) for its practicality. And it is also considered as one of the most valuable medical books for basic medical studies. As 許浚(Heo Jun) mentioned on the 東醫寶鑑(DongEuiBoGam) preface. we should practice flexibility on the interpretation of the medical classics instead of binding ourselves to the precedents. But worship of 東醫寶鑑(DongEuiBoGam) may hinder the studying of new pathways. The treatment and its understandings of a disease should be based on objectivity of observation. Finding the common traits of the recipes in 東醫寶鑑(DongEuiBoGam) is important for the study of 東醫寶鑑(DongEuiBoGam). Accordingly, group synthesis of symptoms and resultant classification of recipes are vital. In this research, symptoms listed in the head part(頭門) of External Shape vol. I(外形篇 卷1) of 東醫寶鑑(DongEuiBoGam) are synthesized into 3 different parts: head-wind syndrome(頭風證), dizziness(眩暈), headache(頭痛). Classification of prescriptions according to these 3 parts is also suggested.

• Journal of the Korean Society of Clothing and Textiles
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• v.2 no.1
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• pp.177-183
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• 1978

Byul-Gam is a low-ranking official who takes charge of sundry services in Yi-Dynasty court, and escorts his king when he make a honored going. Uniforms of Byul-Gam are noted for their gaiety. This study aims at inquiring into uniforms of Byul-Gam through historical records. The resultant findings are as follows: 1) Uniforms of Byul-Gam are divided into full dress and ordinary clothes. 2) The full dress has two kinds of style. One is Dan-Ryung, a kind of robe, clad together with Gun as a headdress. This robe is blue, Gun for king's Byul-Gam violet, and Gun for the Crown Prince's Byul-Gam blue. The other is a combination of yellow Cho-Rip and red Chup-Ri. This is a military uniform. 3) Ordinary dresses for Byul-Gam are orange Cho-Rip and red Jik-Ryung. Underwear in this case is Hyup-Joo-Um and Chup-Ri. Hyup-Joo-Um is a kind of robe. Inside it Korean style of jacket and slacks are usually clad. 4) As time passed, the uniform of Byul-Gam had been changed from Cheong-Dan-Ryung to Hong-Dan-Ryung, and Jik-Ryung had been shifted to a full dress. 5) Only five remains of Jik-Ryung for Byul-Gam have been handed down to us. The analysis of those indicate that they gradually became similar to Duru-Magi, a kind of Korean robe. This fact is common in the other transfiguring process of dresses of robe kind.

• The Journal of Korean Medical History
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• v.17 no.1
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• pp.177-194
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• 2004

This study examines the main points of DongUiBoGam(東醫寶鑑) based on the preface(序) and Compiled Examples(集禮) of DongUiBoGam(東醫寶鑑). The results of this study are the following: 1) DongUiBoGam(東醫寶鑑)'s InSinReon(人身論: man-body doctrine) states that Form(形 -hyeong) and Jeong(精), Ki(氣), Sin(神) are the foundation and the Organs are the mediators. 2) DongUiBoGam(東醫寶鑑) includes Taoism, Buddhism, and Medical Science. 3) DongUiBoGam(東醫寶鑑)'s NaeGyeongChapter(內景篇), OeHyeongChapter(外形篇), JapByeongChapter(雜病篇)'s name shows that each chapter's key concept is Gyeing, Hyeong(形-Form), and Byeong(病-Sickness). 4) The purpose of DongUiBoGam(東醫寶鑑) is Following the Ideas Handed Down From Forefathers(慕古人之遺意), and in this prospect, the context could be named, 'The Treasure Box and Mirror of Eastern Ideas' (東意寶鑑-DongUiBoGam).

• The Journal of Pediatrics of Korean Medicine
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• v.24 no.1
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• pp.65-76
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• 2010

Objectives In the later period of Choseon dynasty, korean pediatrics became a pivot part of medicine. SoAUiBang was the most important book in that era. DongEuiBoGam was the most widely read of all oriental medicine books and published in early days of the Choseon dynasty. Therefore, this comparative study on the prescription and formation of SoAUiBang and DongEuiBoGam could be a useful way to understand SoAUiBang's characteristics. Methods This study was based on SoAUiBang and DongEuiBoGam, two literatures of oriental medicine. Results 1. Formation of SoAUiBang had a similar aspect with that of DongEuiBoGam's and enlarged the area of pediatrics by featuring general medical books. However, its formation did not follow the DongEuiBoGam's, but had its unique formation. It tried to include all diseases of pediatrics in the system of DongEuiBoGam. Plus, smallpox and measles belonged to the epidemic diseases were excluded from the area of pediatrics. 2. Comparing SoAUiBang's prescription of some important diseases with DongEuiBoGam's, it was almost a prescription based on DongEuiBoGam's prescription. However, adjustment of herbs and dosages could not be found in any oriental medicine books including DongEuiBoGam. Conclusions SoAUiBang maintained the traditional Oriental medicine based on author's scholarly creativeness. In addition, it also inherited the lineage of medicine in Korea along with the pedigree of DongEuiBoGam. Therefore, SoAUiBang should be re-evaluated as a medicine book that opened a new field of pediatric and had great academic values.

• Preventive Nutrition and Food Science
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• v.3 no.1
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• pp.67-70
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• 1998

To investigate an effect of Gam-Roa tea on the free radical or alcohol detoxicating enzyme activities, the rats got a drink at the Gam-Roa tea instead of water for 3 months, and then the animals were sacrificed and obtained the following findings. The animals receiving Gam-Roa tea showed a decreasing tendency of hepatic xanthine oxithine oxidase activity and significantly incresed content of cytochrome P-450 compared with the control. Furthermore, hepatic superoxide dismutase and glutathione S-transferase activities were also more increased in rats received Gam-Roa tea than in the control group, those receiving water. On the other hand, alcohol or aldehyde dehydrogenase activities were more increased in rats receiving Gam-Roa tea than the control. In conclusion, it is likely that the liver of rats receiving Gam-Roa tea may have the oxygen free radical or alcohol detoxication potential.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.24-30
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• 2004

The immune activities of Ganoderma lucidum Mycelium added garlic extracts (GAM), Ganoderma lucidum Mycelium (GM), garlic extracts (GS) and standard $({\beta}-glucan)$ were compared. GAM enhanced the growth of human immune T cell up to $1.25{\sim}1.46$ times, compared to control group. GAM showed relatively lower cytotoxicity in using normal human lung cell, while GAM showed the most potent inhibitory effect on the human lung carcinoma, compared to GM and GS. The selectivity of GAM was also higher than that of GM and GS. GAM increased the secretion of cytokines, IL-6 and TNF- from human B cell as well as the growth of human immune cells. It can imply that GAM has higher immune activity than GM or GS.

• The Journal of Korean Medical History
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• v.15 no.1
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• pp.147-182
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• 2002

By studing the congestion theory of Choo Dan-kyei mentioned in "DongEuiBoGam", I have reached following conclusions. 1. Dan-kyei's congestion theory influenced greatly on "DongEuiBoGam". The congestion theory of "DongEuiBoGam" is built up with several theories centering round on Dan-kyei's theory and Huh-Jun's own opinion in addition. 2. Dan-kyei's congestion theory has given theoretical foundation to the classification and diagnosis of congestion reported in "DongEuiBoGam"-Congestion Chapter. 3. Dan-kyei's congestion theory offers many examples of symptoms reported in "DongEuiBoGam"-Congestion Chapter. It's shown well through outer symptoms, several congestion disease, congestion mass, and "congestion never sudden death", etc. 4. Dan-kyei's congestion theory has influenced greatly on treatment of congestion reported in "DongEuiBoGam"-Congestion Chapter, and it's well shown through spewing method, grand method in congestion treatment and common medicine of congestion, etc. 5. Dan-kyei's congestion theory offers variety of treating methods to symptoms caused by congestion reported in "DongEuiBoGam". It's shown variously through upper stomachache, stomachache, back pain, side pain, "San", joint pain, Summer disease, internal injury, cough and morning sickness, etc.. 6. Dan-kyei's congestion theory has factors that could be operated to common diseases of mordern people. They can be classified as food congestion, alcohol congestion, obstruction congestion and Qi congestion, and they are clinically very efficient.

• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.33-43
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• 2015

GAM-1 and GAM-2, two themostable glucoamylases from Aspergillus niger B-30, possess different molecular masses, glycosylation, and thermal stability. In the present study, the effects of additives on the thermal inactivation of GAM-1 and GAM-2 were investigated. The half-lives of GAM-1 and GAM-2 at 70℃ were 45 and 216 min, respectively. Data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, UV absorption spectroscopy, and dynamic light scattering demonstrated that during the thermal inactivation progress, combined with the loss of the helical structure and a majority of the tertiary structure, tryptophan residues were partially exposed and further led to glucoamylases aggregating. The thermal stability of GAM-1 and GAM-2 was largely improved in the presence of sorbitol and trehalose. Results from spectroscopy and Native-PAGE confirmed that sorbitol and trehalose maintained the native state of glucoamylases and prevented their thermal aggregation. The loss of hydrophobic bonding and helical structure was responsible for the decrease of glucoamylase activity. Additionally, sorbitol and trehalose significantly increased the substrate affinity and catalytic efficiency of the two glucoamylases. Our results display an insight into the thermal inactivation of glucoamylases and provide an important base for industrial applications of the thermally stable glucoamylases.

• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.147-154
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• 2013

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and $100{\mu}g/chick$). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with $5{\times}10^4$ sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.