• Title/Summary/Keyword: G-Rh2

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Local Irritation Test of HM10411 (rhG-CSF) in Rabbits (인과립구 콜로니 자극인자 제제인 HM10411 (rhG-CSF)의 국소자극성)

  • 차신우;한정희;김충용;서정은;김종춘;권세창
    • Biomolecules & Therapeutics
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    • v.10 no.3
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    • pp.170-174
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    • 2002
  • The local irritation study (skin and occular irritation tests) of HM10411, a rhG-CSF (recombinant human granulocyte-colony stimulating factor) was carried out in New Zealand White rabbits. HM10411 was applied to the bare skin at a dose of 2.5 mg/rabbit (5.0 mg/ml, 0.5 ml) and to the conjunctival sac of eye at a dose of 0.5 mg/rabbit (5.0 mg/ml, 0.1 ml) , respectively. In this study, there were no clinical signs which were related to HM10411 compared with those of control group. From above results, HM10411 has not any irritating activity to skin and eye in rabbits.

항암화학요법에 의하여 골수억제가 수반된 진행암 환자에서 Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor(rhGM-CSF)의 용량과 효과에 관한 비교 연구

  • 노재경;라선영;이경희;이혜란;정현철;김주항;김병수
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.306-306
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    • 1994
  • 항암화학요법후 가장 심각한 부작용의 하나는 중성구 감소에 의한 감염이다. 본원에서는 rhGM-CSF을 이용한 제 I상 임상연구에서 150-500$\mu$g/M$^2$/day가 biologically active dose임을 보고한 바 있다. 연자들은 연세암센터에 내원하여 진행성 악성종양으로 병리조직학적 진단을 받고 항암화학요법시행후 골수억제가 예상되는 환자를 대상으로 GM-CSF 용량에 따른 안전성 및 독성을 검토하고 백혈구 감소증 및 감염의 예방, 치료효과를 분석하여 임상사용권장량을 결정하기위한 2상 연구를 대상환자의 동의를 얻은후 시행하였다. 대상환자는 37명 (여 26, 남 11)이었고, 항암제는 Adriamycin, Cisplatin, VP-16이 주로 사용되었다. 최적임상사용권장량을 결정하기 위하여 1500$\mu\textrm{g}$/M$^2$/day을 12명, 250$\mu\textrm{g}$/M$^2$/day을 12명, 350$\mu\textrm{g}$/M$^2$/day을 13명의 환자에게 투여하였다. 첫번째 항암 요법에는 rhGM-CSF을 투여하지않고 (비투여기) 두 번째 항암요법에서는 항암요법후 익일부터 10일간 연속, 매일 1회 피하주사하여 (투여기), rhGM-CSF 투여기와 비투여기의 백혈구 감소증 정도의 차이를 비교하였다.

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항암화학요법에 의하여 골수억제가 수반된 진행암 환자에서 Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor(rhGM-CSF)의 용량과 효과에 관한 비교 연구

  • 노재경;라선영;이경희;이혜란;정현철;김주항;김병수
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.330-330
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    • 1994
  • 항암화학요법후 가장 심각한 부작용의 하나는 중성구 감소에 의한 감염이다. 본원에서는 rhGM-CSF을 이용한 제 I상 임상연구에서 150-500$\mu$g/M$^2$/day가 biologically active dose임을 보고한 바 있다. 연자들은 연세암센터에 내원하여 진행성 악성종양으로 병리조직학적 진단을 받고 항암화학요법 시행후 골수억제가 예상되는 환자를 대상으로 GM-CSF 용량에 따른 안전성 및 독성을 검토하고 백혈구 감소증 및 감염의 예방, 치료효과를 분석하여 임상사용권장량을 결정하기위한 2상 연구덜 대상환자의 동의를 얻은후 시행하였다. 대상환자는 37명 (여 26, 남 11)이었고, 항암제는 Adriamycin, Cisplatin, VP-l6 이 주로 사용되었다. 최적임상사용권장량을 결정하기 위하여 1500$\mu\textrm{g}$/M$^2$/day을 12명, 250$\mu\textrm{g}$/M$^2$/day을 12명, 350$\mu\textrm{g}$/M$^2$/day을 13명의 환자에게 투여하였다. 첫번째 항암요법에는 rhGM-CSF을 투여하지않고 (비투여기) 두번째 항암요법에서는 항암요법후 익일부터 10일간 연속, 매일 1회 피하주사하여 (투여기), rhGM-CSF 투여기와 비투여기의 백혈구 감소중 정도의 차이를 비교하였다.

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A Correlation Analysis between Visibility and PM10 in Jinju (진주지역 시정과 미세먼지(PM10) 농도와의 상관관계)

  • Kim, Hyoung-Kab;Suh, Jeong-Min;Park, Jeong-Ho
    • Journal of Environmental Science International
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    • v.26 no.4
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    • pp.535-542
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    • 2017
  • This study was conducted to investigate how $PM_{10}$ concentration and Relative Humidity (RH) affected visibility in Jinju, Korea. A 9-yr dataset of 1 h averages for visibility, $PM_{10}$, and RH data was analyzed to examine the correlation between these variables. On average, visibility decreased by 1.4 km for every $10{\mu}g/m^3$ increase in $PM_{10}$ and by 2.1 km for every 10% increase in RH. In general, a negative correlation was observed between visibility and and $PM_{10}$ concentration. However, under conditions of low $PM_{10}$ concentration(< $15{\mu}g/m^3$) and visibility(< 2 km), there was a positive correlation between these two variables. In this case, RH levels were high (> 75%). A high correlation analysis between two variables need to be under control conditions with RH < 75%, $PM_{10}$ $15{\sim}100{\mu}g/m^3$, and visibility > 2 km.

Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats (Rat의 방사선 조사성 구내염에 대한 Recombinant Human Epidermal Growth Factor (rhEGF)의 효과)

  • Jung Kwon-Il;Kim Sun-Hee;Moon Soo-Young;Kim Yeon-Wha;Hong Joon-Pio;Kim Hyun-Sook;Lee Sang-Wook
    • Radiation Oncology Journal
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    • v.24 no.1
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    • pp.67-76
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    • 2006
  • Purpose: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model. Materials and Methods: Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF-treated groups. Rats were topically applied with rhEGF (15 or $30{\mu}g/oral$ cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation. Results: rhEGF-treated groups (15 or $30{\mu}g/oral$) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats. Conclusion: These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.

Preparation of $Ginsenoside-Rh_2$ from Dammarane Saponins of Panax ginseng Leaves (인삼잎의 Dammarane계 사포닌으로부터 $Ginsenoside-Rh_2$의 제조)

  • Cha, Bae-Cheon;Lee, Sang-Guk
    • YAKHAK HOEJI
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    • v.38 no.4
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    • pp.425-429
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    • 1994
  • The genuine aglycone, 20(S)-protopanaxadiol, obtained from the leaves of Panax ginseng as a result of direct alkaline treatment was isolated and characterized by spectroscopic evidences. The study on the yield of genuine aglycone which is produced from the treatment of some kinds of alkali was carried out. $Ginsenoside-Rh_2$ was synthesized by conjugation of 2,3,4,6-tetra-O-acetyl-${\alpha}$-D-glucopyranosyl bromide to 20(S)-protopanaxadiol in the presence of silver carbonate and cadmium cabonate. The preparation of $ginsenoside-Rh_2$ by this method is a new one which the yield of this saponin can be improved in the mild condition.

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Tissue response of Pro-Root® MTA with rhBMP-2 in pulpotomized rat teeth (백서에서 치수 절단술 시행 시 Pro-Root® MTA 단독 사용군과 rhBMP-2 혼용 사용군 간의 조직 반응 비교 연구)

  • Park, Kyung-Tae;Yang, Won-Kyung;Ko, Hyun-Jung;Kim, Mi-Ri
    • Restorative Dentistry and Endodontics
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    • v.32 no.5
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    • pp.403-410
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    • 2007
  • The purpose of this study was to investigate whether rhBMP-2 (BMP2) could induce synergistic effect with $Pro-Root^{(R)}$ MTA (MTA) in pulpotomized teeth in the rats. Healthy upper first molars from thirty-two, 10 weeks old, Sprague-Dawley rats were used for this investigation. The molars were exposed with round bur, and light pressure was applied with sterilized cotton to control hemorrhage. 1.2 grams of MTA cement was placed in right first molars as a control group. In left first molars, $1\;{\mu}g$ of BMP2 was additionally placed on exposed pulps with MTA. All cavities were back-filled with light-cured glass-ionomer cements. The rats were sacrificed after 2 weeks and 7 weeks, respectively. Then histologic sections were made and assessed by light microscopy. Data were statistically analyzed via student t-test with SPSSWIN 12.0 program (p < 0.05). Inflammation observed in 2 weeks groups were severe compared to the 7 weeks groups. But the differences were not statistically significant. BMP2-addition groups had less inflammation than MTA groups in both periods, though these differences were also not statistically significant. In conclusion, the combination of BMP2 and MTA showed no differences with MTA only for pulpotomy of rat teeth.

Conception Rate of Ovulation-Estrus Synchronization Method in Hanwoo (한우에 있어서 발정ㆍ배란 동기화법에 의한 수태율)

  • 박정준;임석기;이명식;전기준;박수봉;정영훈;우제석;나기준
    • Korean Journal of Animal Reproduction
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    • v.27 no.3
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    • pp.207-213
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    • 2003
  • This objective of this experiment were to evaluate the effect of various estrus synchronization programs on estrus detection rate and pregnancy rate in Hanwoo. After Postpartum 60 Days, a total of 150 cows divided into 2 groups. Cows Group 1 were treated with one luteolytic dosage of PGF$_2$$\alpha$(25 mg, im; lutalyse. USA) on Day 0, and with a second dosage 14 d later; cows in Group 2 were treated with GnRH(l00 $\mu\textrm{g}$, im; Conceral. Korea) on Day 0, PGF$_2$$\alpha$ 7 d later, GnRH 2 d later, and then time-inseminated approximately 16 h after this second treatment with GnRH. Ovarian morphology was monitored cows by trans-rectal ultrasonography from 24 hr to 32 hrs after second GnRH injection. The result obtained summarized as follows: 1. Cows synchronization of estrus with GnRH+PGF$_2$$\alpha$+GnRH(Ov-synch) and PGF$_2$$\alpha$ were 91.3 and 40.0%, respectively. 2. Induced ovulation were 24 to 32hr after the second GnRH injection, but high induced ovulation was 28hr. 3. High conception rate were 24hr insemination after the second GnRH injection. 4. Conception rate with PGF$_2$$\alpha$, CIDR and GnRH treatment were 50.0, 36.0 and 76.9%, respectively.

Decolorization of Rhodamine B Using UV/$TiO_2$ System (UV/$TiO_2$ 시스템을 이용한 Rhodamine B의 색도 제거)

  • 박영식;나영수;안갑환
    • Journal of Environmental Health Sciences
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    • v.28 no.5
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    • pp.59-64
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    • 2002
  • The photocatalytic decolorization of the Rhodamine B (RhB) was studied using a UV/TiO$_2$ reactor. Yakuri titanium dioxide(anatase) was used as the suspended photocatalyst and proved to be effective for decolorization irradiated with UV light (254 mm). The photocatalyzed dioxide concentrations, light intensity and air flow rates. In 0.01 mM RhB, color could be completely photodegraded after 3 hours. Absorption spectrum of an aqueous solution containing RhB showed a continued diminution of the RhB concentration in the solution bulk : concomitantly, no new absorption peaks appeared. This confirmed the decolorization of RhB, i.e., the break up of the chromopore. The optimum loaded titanium dioxide for the decolorization was 0.75 g/(equation omitted). The light intensity showed exponential decay with distance. The decay of light intensity of RhB solution showed different tendency from TiO$_2$. These results suggested that the photocatalytic decolorization of dyes may be available method for decolorizing in wastewater.

Isolation of Ginsenoside${-Rh}_1$ and ${-Rh}_2$ by High Performance Liquid Chromatography (고속액체(高速液體) 크로마토그래피에 의(依)한 Ginsenoside ${-Rh}_1$${-Rh}_2$ 의 분리(分離))

  • Choi, Jin-Ho;Kim, Woo-Jung;Hong, Soon-Keun;Oh, Sung-Ki;Oura, Hikokichi
    • Korean Journal of Food Science and Technology
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    • v.13 no.1
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    • pp.57-66
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    • 1981
  • An effective method for isolation of the major components of ginseng saponin such as $ginsenoside-Rb_{1},\;-Rb_2,$ -Rc, -Rd, -Re and $-Rg_1$, and the minor components such as ginsenoside-Rf, $-Rg_2,\;and-Rh_1$, was developed and reported in previous papers (J. Korean Agr. Chem. Soc., 23(4), 199 and 206(1980) The conditions and procedures used for isolation and identification for ginsenosides described in the previous papers were not sufficient enough for clean separation of minor components, $ginsenoside-Rh_1,\;and-Rh_2$. In this work, modifications in extraction method and in mobile phase for HPLC were attempted. It was found that application of ethyl acetate extraction at $60^{\circ}C$ for 3 hr on crude saponin resulted in a removal of diol group saponin from crude saponin which made it possible for using higher portion of acetonitrile in mobile phase. The mixed solvents of acetonitrile : water (92 : 8 and 94 : 6) gave excellent resolution of $ginsenoside-Rh_1\;and\;-Rh_2$.

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