• 제목, 요약, 키워드: G-Rh2

검색결과 390건 처리시간 0.061초

개에서 재조합 과립구 자극 인자 (rh G-CSF)의 투여 간격에 따른 말초혈액과 골수의 반응에 대한 연구 (A Study of Peripheral Blood and Bone Marrow Responses Depends on the Frequency of rhG-CSF Administration in Dogs)

  • 김지현;지초희;원진희;정해원;문종현;조규완;강병택;정동인
    • 한국임상수의학회지
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    • v.31 no.2
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    • pp.77-84
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    • 2014
  • 본 연구는 사람 재조합과립구자극인자 (rhG-CSF) 투여 간격에 따른 말초혈액과 골수의 변화를 평가하였다. 사람 재조합과립구자극인자는 개에서 항암치료 후 나타나는 골수억압 등에서 유용한 치료효과가 있는 것으로 알려져 있으나 그 투여간격에 따른 연구는 아직 진행되지 않았다. 따라서 본 연구에서는 다음과 같은 간격으로 실험 기간 동안 각각 2마리씩 3그룹으로 체중당 $5{\mu}g$으로 사람 재조합과립구자극인자를 주사하였다; (1) 하루에 한번 주사; 그룹 1, (2) 2일에 한번 주사; 그룹 2, (3) 3일에 한번 주사; 그룹 3, 말초혈액 분석과 100개의 직접 현미경 세포계수는 매일 실시하였다. 골수천자는 0일차, 3일차 9일차 마지막으로 말초혈액상 총 백혈구수가 정상범위내로 떨어지는 12일차 혹은 13일차에 시행하였다. 사람 재조합과립구자극인자는 모든 실험견에서 잘 투여되었으며 부작용을 보이지 않았다. 이러한 본 연구의 결과에 따르면 체중당 $5{\mu}g$의 사람 재조합과립구자극인자의 투여는 말초혈액과 골수에서 세포특이적이고 투여간격에 대체적으로 비례하는 효과를 보였으며 사람 재조합과립구자극인자 투여가 반복될수록 그 효과는 증가하였으며 사람 재조합과립구자극인자 투여를 중지한 이후에도 골수에 미치는 효과는 3일이상 지속되는 것으로 보여졌다. 따라서 본 연구의 결과를 통해 임상가들은 적절한 치료 간격을 세울 수 있을 것으로 기대된다.

Ultrasonic Synthesis of CoSe2-Graphene-TiO2 Ternary Composites for High Photocatalytic Degradation Performance

  • Ali, Asghar;Oh, Won-Chun
    • 한국세라믹학회지
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    • v.54 no.3
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    • pp.205-210
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    • 2017
  • In this study, we examined the photo-degradation efficiency of $CoSe_2$-Graphene-$TiO_2$ ($CoSe_2-G-TiO_2$) nanocomposites under visible light irradiation using rhodamine B (RhB) as standard dye. $CoSe_2-G-TiO_2$ nanocomposites were synthesized by ultrasonication and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopic analysis and UV-Vis absorbance spectra analysis. Our results show that the $CoSe_2-G-TiO_2$ nanocomposite exhibited significant photo degradation efficiency compared to pure $TiO_2$ and $CoSe_2-G$, approximately 85.2% of the rhodamine (Rh B) degraded after 2.5 h. It is concluded that the $CoSe_2-G-TiO_2$ nanocomposite is a promising candidate for use in dye pollutants.

생식소자극호르몬방출호르몬 유사물질에 의한 메기(Silurus asotus)의 배란유도 (Induced Ovulation in Catfish (Silurus asotus) by GnRH-Analogue)

  • 권혁추;최낙중;박홍양
    • 한국양식학회지
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    • v.9 no.3
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    • pp.205-213
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    • 1996
  • 한국산 메기의 경제적이고 효율적인 산란유도를 위한 연구로써 GnRH-a의 이용가능성에 대하여 검토하여 다음과 같은 결과를 얻었다. GnRH-a 처리된 메기의 산란유도율은 어체중 kg당 70 ${\mu}g$에서 $67\%$, 90 ${\mu}g$에서 $86\%,\;120{\mu}g$이상에서는 $100\%$로 나타났다. 호르몬 주사후 산란이 유도되기까지 대체로 $22\~25$시간이 소요되었다. 생식소중량지수(GSI)는 $100\%$의 산란유도율을 보인 120 ${\mu}g/kg$처리된 그룹에서 $23\~30\%$를 pseudo-GSI는 $18\~21\%$로 비교적 높고 고른 분포를 나타냈으며, 산란된 난의 수는 어체중 kg당 $58,000\~65,000$개 였다. 또한 수정률 및 부화율은 각각 $94\%$$81\%$로 나타났다. GnRH-a 처리에 따른 뇌하수체의 미세구조적 변화를 관찰한 바, 호르몬 주사전 성숙한 암컷 메기의 생식소자극호르몬 분비세포(gonadotrops)는 전자밀도가 높은 $150\~300$ nm 크기의 수 많은 소과립과 $800\~1000\;nm$의 전자밀도가 다소 낮은 소수의 대과립의 존재가 관찰되었다. 한편 호르몬주사후의 gonadotrops에서는 대소 과립들의 현저한 소실과 rER의 현저한 증가가 관찰되었는데, 이는 GnRH-a에 의해 생식소자극호르몬의 대량방출을 시사하는 것이다. 이상의 결과로부터 GnRH-a의 사용은 기존의 HCG 및 잉어뇌하수체 분말보다 적어도 $2\~3$ 배이상의 비용절감을 가져와 메기의 인공 종묘 생산에 매우 효과적이고 경제성이 있는 것으로 나타났다.

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3차원 전극을 사용한 Rhodamine B의 전기분해에 미치는 운전인자의 영향 (Effect of Operating Parameters on Electrochemical Degradation of Rhodamine B by Three-dimensional Electrode)

  • 김동석;박영식
    • 한국환경보건학회지
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    • v.35 no.4
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    • pp.295-303
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    • 2009
  • A simulated wastewater containing the dye Rhodamine B (RhB) was electrolytically treated using a three-dimensional electrode reactor equipped with granular activated carbon (GAC) as particle electrode. The effect of type of packing material (GAC, ACF, Nonwoven fabric fiber coated with activated carbon), amounts of GAC packing (25-100 g), current (0.5-3 A) and electrolyte concentration (0.5-3 g/l) was evaluated. Experimental results showed that performance for RhB decolorization of the 3 three-dimensional electrodes lie in: GAC > Nonwoven fabric fiber > ACF. When considered RhB decolorization, oxidants concentration and electric power, optimum GAC dosage was 50 g. Generated concentration of 3 oxidants ($ClO_2$, free Cl, $H_2O_2$) was increased with increase of applied current, however optimum current for RhB degradation was 2.5 A. The oxidants concentration was increased with increase of NaCl concentration and optimum NaCl dosage for RhB degradation was 1.5 g/l.

Gonadotropin-Releasing Hormone의 투여(投與)가 산욕기(産褥期)의 유우(乳牛)와 난소낭종유우(卵巢囊腫乳牛)의 번식효율(繁殖效率)에 미치는 영향(影響) (Effects of Gonadotropin-Releasing Hormone on Reproductive Performance of Early Postpartum Dairy Cows and Cystic Cows)

  • 황우석
    • 대한수의학회지
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    • v.21 no.1
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    • pp.59-64
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    • 1981
  • Holstein-Friesian cows(n=284) were given $100{\mu}g$ of gonadotropin-releasing hormone(GnRH) or saline solution by intramuscular injection at 10 to 22 days after parturition, and were investigated their reproductive performance and frequency of ovarian cysts. Among them 28 cystic cows were injected with $150{\mu}g$ of GnRH intramuscularly and examined the recovery rate. The results obtained in this study were summarized as follows: 1. The interval from calving to 1st ovulation was reduced from 28.2 days in controls to 16.5 days for cows given GnRH (p<0.01). 2. The intervals from calving to 1st estrus and from calving to conception were extended significantly in control group (p<0.05). 3. Inseminations per conception and conception rate at 1st insemination did not reveal difference between two groups. 4. Frequency of ovarian cysts was reduced from 14.0% in control to 4.20% for cows given GnRH (p<0.05). 5. Of the 28 cystic cows receiving $150{\mu}g$ of GnRH, 23(82.1%) responded to 1st treatment and returned to estrus $24.2{\pm}4.3$ days after treatment. 6. These data provide evidence for reduction in infertility and reproductive disorders in early postpartum dairy cows given GnRH as a prophylactic.

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Optimization of Extracellular Production of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) with Bacillus subtilis

  • Kim, Chun-Kwang;Rhee, Jong Il
    • Journal of Microbiology and Biotechnology
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    • v.24 no.2
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    • pp.188-196
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    • 2014
  • Extracellular production of recombinant human bone morphogenetic protein-7 (rhBMP-7) was carried out through the fermentation of Bacillus subtilis. Three significant fermentation conditions and medium components were selected and optimized to enhance the rhBMP-7 production by using the response surface methodology (RSM). The optimum values of the three variables for the maximum extracellular production of rhBMP-7 were found to be 2.93 g/l starch, 5.18 g/l lactose, and a fermentation time of 34.57 h. The statistical optimization model was validated with a few fermentations of B. subtilis in shake flasks under optimized and unoptimized conditions. A 3-L jar fermenter using the shake-flask optimized conditions resulted in a higher production (413 pg/ml of culture medium) of rhBMP-7 than in a shake flask (289.1 pg/ml), which could be attributed to the pH being controlled at 6.0 and constant agitation of 400 rpm with aeration of 1 vvm.

Hypophyseal and Gonadal Response to GnRH in Buffalo Heifers (Bubalus bubalis)

  • Singh, C.;Madan, M.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.4
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    • pp.416-421
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    • 1998
  • The objective of this study was to investigate the responsiveness of hypophysis and gonads to synthetic GnRH among noncycling Murrah buffalo heifers at 24 months of age. The plasma FSH, LH, estradiol and progesterone levels were measured in blood samples collected at 1 hour before and upto 18th day subsequent to the administration of GnRH ($(200 {\mu}g)$) or saline (2 ml). The pretreatment levels of plasma FSH, LH estradiol and progesterone among GnRH treated heifers (N = 6) were $11.55{\pm}0.57ng/ml$, $0.68{\pm}0.06ng/ml$, $19.84{\pm}0.82pg/ml$ and $0.45{\pm}0.07ng/ml$ respectively. A quick elevation of FSH (p < 0.01) and LH (p < 0.05) within 5 min of GnRH administration was observed in all the heifers. The peak FSH ($74.97{\pm}18.63ng/ml$) and LH ($3.09{\pm}0.54ng/ml$) level was obtained at 30 min of GnRH administration. The elevated level of plasma estradiol on 5th to 18th day, FSH on 7th to 9th day (n = 3) and the progesterone on 13th to 18th day (n = 2) of GnRH injection was obtained. The study indicates that gonads of buffalo heifers at 24 months of age are responsive of GnRH induced gonadotropin release for folliculogenesis and luteal tissue formation

3차원 전극을 이용한 염료의 색 제거

  • 박영식;김동석
    • 한국환경과학회:학술대회논문집
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    • pp.213-216
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    • 2008
  • 2차원 전극의 RhB 분해 성능은 Pt(+)-Pt(-) > Pt(+)-Sus(-) > Sus(+)-Sus(-) 전극의 순으로 나타났다. 3차원 전극은 Pt-ACF-Pt 전극만 2차원 전극보다 초기 반응속도가 낮고 최종 RhB농도가 높은 것으로 나타났으나 다른 전극은 충전물 종류에 관계 없이 2차원 전극보다 초기 반응속도가 높은 것으로 나타났다. 음극의 종류에 관계없이 3차원 전극의 충전물에 따른 RhB 분해는 GAC > Nonwoven fabric fiber > ACF로 나타났다. GAC 충전량이 50 g 이상에서 RhB 분해속도는 거의 유사한 것으로 나타났으나 전력을 고려한 단위 W당 시간당 RhB 농도 감소를 나타내었다. 그림에서 보듯이 최적 GAC 충전량은 50 g로 사료되었다. 최적 전류는 2.5 A로 사료되었다.

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BDD 전극을 이용한 OH 라디칼 생성과 염료 분해에 미치는 운전인자의 영향 (Effects of Operating Parameters on Electrochemical Degradation of Rhodamine B and Formation of OH Radical Using BDD Electrode)

  • 박영식;김동석
    • 한국환경과학회지
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    • v.19 no.9
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    • pp.1143-1152
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    • 2010
  • The purpose of this study is to degradation of Rhodamine B (RhB, dye) and N, N-Dimethyl-4-nitrosoaniline (RNO, indicator of the electro-generation of OH radical) in solution using boron doped diamond (BDD) electrode. The effects of applied current (0.2~1.0 A), electrolyte type (NaCl, KCl, and $Na_2SO_4$) and electrolyte concentration (0.5~3.0 g/L), solution pH (3~11) and air flow rate (0~4 L/min) were evaluated. Experimental results showed that RhB and RNO removal tendencies appeared with the almost similar thing, except of current. Optimum current for RhB degradation was 0.6 A, however, RNO degradations was increased with increase of applied current. The RhB and RNO degradation of Cl type electrolyte were higher than that of the sulfate type. The RhB and RNO degradation were increased with increase of NaCl concentration and optimum NaCl dosage was 2.5 g/L. The RhB and RNO concentrations were not influenced by pH under pH 7. Optimum air flow rate for the oxidants generation and RhB and RNO degradation were 2 L/min. Initial removal rate of electrolysis process was expressed Langmuir - Hinshelwood equation, which is used to express the initial removal rate of UV/$TiO_$2 process.

Disinfection of Penicillium-infected Wheat Seed by Gaseous Chlorine Dioxide

  • Jeon, Young-ah;Lee, Ho-sun;Lee, Young-yi;Lee, Sokyoung;Sung, Jung-sook
    • 식물병연구
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    • v.21 no.2
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    • pp.45-49
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    • 2015
  • Seeds of wheat (Triticum aestivum L. cv. Olgeurumil) were infected with Penicillium sp. at mean infection rate of 83%. Penicillium sp. was detected in endosperm with bran but not in embryo. Gaseous chlorine dioxide ($ClO_2$) effectively inhibited growth of Penicillium sp. at concentration of 5 to $20{\mu}g/ml$. As treatment duration was extended from 1 to 3 h, growth of Penicillium sp. was completely suppressed even at $10{\mu}g/ml$. There was no significant reduction in the incidence of Penicillium sp. at 30% relative humidity (RH). However, the incidence of Penicillium sp. was 27.7% at 50% RH, further those were 3.5% and 0.2% at 70% and 80% RH, respectively. Seed germination was not affected by $ClO_2$ treatment at all the RH conditions. Water-soaked seeds (30% seed moisture content) showed a drastic reduction in the incidence of Penicillium sp. when treated at more than $10{\mu}g/ml$ of $ClO_2$. The incidences of Penicillium sp. were 3.3, 1.8 and 1.2% at 10, 15 and $20{\mu}g/ml$, respectively. The incidence of Penicillium sp. in dry seeds with 9.7% seed moisture content did not reduce when treated with 5 and $10{\mu}g/ml$ at 50% RH although it tended to decrease as $ClO_2$ concentration increased to $20{\mu}g/ml$. Seed germination was not affected by $ClO_2$ treatment at the tested concentrations. These results indicated that gaseous $ClO_2$ was effective disinfectant to wheat seeds infected with Penicillium sp. and that the effectiveness of $ClO_2$ strongly increased when moisture content around or inside of the seed was increased.