• Title/Summary/Keyword: G-6-PD

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Effects of Modified Atmosphere Packaging on the Quality of Chitosan and $CaCl_2$ Coated Mushroom (Agaricus bisporus) (환경가스조절 포장이 키토산 및 $CaCl_2$로 코팅 처리된 양송이 버섯의 품질에 미치는 영향)

  • Lee, Jin-Sil
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1308-1314
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    • 1999
  • The effects of modified atmosphere(MA) packaging and coating on the quality of mushroom (Agaricus bisporus) were investigated. Whole mushrooms(100 g) were packed with polyvinyl chloride(PVC) film wrap, PD941 and PD961 film bags and were stored in a chamber at $12^{\circ}C$ and 80% RH for 6 days. Gas composition of packages, respiration rate, weight loss, color and maturity index of MA packaged mushrooms were examined. The wrap packaged mushrooms showed different level of internal $CO_2$ concentrations among water washed, chitosan coated and $CaCl_2$ coated subgroups significantly at 5% level. The maximum $CO_2$ concentration of PD941 and PD961 packages for all coated showed $4.1{\sim}4.7%$ and $10.4{\sim}11.7%$, respectively, for the first day. PD961 package showed the lowest $O_2$ concentration compared to other groups. There were no significant respiration rate differences between wrap packed and PD941 packaged. Respiration rate of water washed. chitosan coated and $CaCl_2$ coated in PD961 packaged was 192 mg, 226 mg and 245 mg, respectively. Maturity index of PD961 packaged were not significantly different among the water washed, chitosan and $CaCl_2$ coated packages. Chitosan coating showed a negative effect on color change of mushrooms. The weight loss of 961 packaged was lower $(7.0{\sim}8.0%)$ that those of wrap packaged and PD941 packaged.

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Influence of Level of Feed Intake on Concentration of Purine Derivatives in Urinary Spot Samples and Microbial Nitrogen Supply in Crossbred Bulls

  • George, S.K.;Dipu, M.T.;Mehra, U.R.;Verma, A.K.;Singh, P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1291-1297
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    • 2006
  • The potential of the spot urine sampling technique as an alternative to performing a total urine collection to predict the microbial nitrogen supply was evaluated in crossbred bulls. In a completely randomized design, 20 growing crossbred bulls were assigned four levels of feed intake (120, 100, 80 and 60% of voluntary dry matter intake) on diets comprised of wheat straw and concentrate mixture (50:50). After three months of experimental feeding, a metabolism trial was conducted for ten days, during which spot urine collections were performed every 6 h post feeding on days 9 and 10. The daily urinary excretion of allantoin (A) and purine derivatives (PD) decreased with the reduction in feed intake while creatinine (C) excretion remained similar in animals fed at different levels. The microbial nitrogen (MN) supply calculated from the PD excreted in total urine (35.08 to 72.08 g/d) was higher at increased levels of feed intake. PD concentration in spot urine samples had poor correlation with feed intake except at 12 h post feeding. A/C ratio and PD/C ratio in spot urine samples remained similar irrespective of sampling time and significantly (p<0.01) correlated with daily urinary PD excretion, digestible organic matter intake and dry matter (DM) intake. However, no significant differences were evident in these ratios among animals fed at levels 120, 100 and 80% of voluntary dry matter intake (VDMI) at different times post feeding. These results suggests that the spot urine sampling technique to predict the microbial protein supply is not suitable for detecting small differences in MN supply and hence, estimation of PD excreted in total urine (mmol/d) is necessary to assess precisely the MN supply in crossbred bulls.

Modified Atmosphere Packaging of Minimally Processed Cut Garlic (최소가공된 절단 마늘의 환경기체조절포장)

  • Kwon, Min-Ji;Shin, Yong-Jae;Lee, Dong-Sun;An, Duck-Soon
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.17 no.1
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    • pp.13-17
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    • 2011
  • There is a need in food industry to store minimally processed garlic for long time to have it be used just at the time of demand for final product processing. Optimal modified atmosphere packaging is expected to slow down the quality change extending its storage life. In order to find optimal packaging conditions, plastic films of different gas permeability properties (low density polyethylene (LDPE) $30{\mu}m$, polyolefin $50{\mu}m$ (PD 900), polyolefin $20{\mu}m$ (PD 941)) were used for packaging 400 g of minimally processed garlic. Perforated LDPE packages were prepared as control. The packaged products were stored at $1{\pm}1^{\circ}C$ for 52 days. Package treatments were compared in weight loss, decay, surface color, hardness and soluble solid content. While control package had normal atmosphere of air, LDPE, PD 900 and PD 941 packages attained internal concentration of $O_2$ 4.6% / $CO_2$ 12%, $O_2$ 0.9% / $CO_2$ 21% and $O_2$ 0.5% / $CO_2$ 13% after 45 days, respectively. Control packaging had rapid weight loss with high mold decay and great surface color change in 45 days. In PD 900 film packages of lowest gas permeability, the fresh-cut garlic could be stored without mold decay for 52 days. Except control packaging, there were no significant differences in surface color, hardness and soluble solid content among package treatments.

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Measurement of Microbial Protein Supply in Murrah Buffaloes (Bubalus bubalis) Using Urinary Purine Derivatives Excretion and PDC Index

  • Dipu, M.T.;George, S.K.;Singh, P.;Verma, A.K.;Mehra, U.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.347-355
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    • 2006
  • A study was conducted to predict the rumen microbial protein production based on urinary excretion of purine derivatives in buffaloes fed a diet of wheat straw and concentrate (40:60) at four fixed levels of feed intake. (95, 80, 60 and 40% of preliminary voluntary feed intake) following experimental protocol of IAEA (Phase I). The buffaloes were allocated according to a $4{\times}4$ latin square design. The urinary allantoin, uric acid, total PD excretion (mmol/d) in treatments L-95, L-80, L-60 and L-40 was 20.13, 16.00, 12.96 and 9.17; 1.88, 2.12, 2.11 and 2.15; 22.01, 18.12, 15.07 and 11.32, respectively and were significantly (p<0.05) different among treatments except for uric acid. The rate of PD excretion (mmol/d) was positively correlated with the digestible organic matter intake. Variations were observed in PD and creatinine concentration in spot samples collected at 6-hour interval. However, daily PD:Creatinine ratio (PDC index) appears to be a reasonably good predictor of microbial-N supply. The contribution of basal purine excretion to total excretion of purine derivatives (PD) was determined in pre-fasting period followed by a fasting period of 6 d (Phase II). Daily PD and creatinine excretion (mmol/kg $W^{0.75}$) during fasting averaged 0.117 and 0.456 respectively for buffaloes. The excretion rates of PD decreased significantly (p<0.01) during fasting compare to pre-fasting period, the urinary creatinine excretion remained almost similar. Except for creatinine, plasma concentration of target parameters significantly (p<0.01) declined during fasting. Likewise, glomerular filtration rate (GFR) and renal clearance of allantoin and uric acid also decreased. Based on the PD excretion rates during fasting and at different levels of feed intake obtained in this study, a relationship between daily urinary PD excretion (Y-mmol) and microbial purine absorption (X-mmol) was developed for buffaloes as Y = 0.74X+0.117 kg $W^{0.75}$. The microbial N supply (g/kg DOMI) remained statistically similar irrespective of dietary treatment. The results showed that excretion of urinary purine derivatives is positively correlated with the levels of feed intake in Murrah buffaloes and thus, estimation of urinary purine derivatives and PDC index could be used to determine microbial nitrogen supply when there is large variation in level of feed intake.

A Simple Metric for Assessing the Severity of Partial Discharge Activity Based on Time-Sequence-Analysis-Discharge Level Patterns

  • Stewart Brian G;Yang Lily;Judd Martin D;Reid Alistair;Fouracre Richard A
    • Transactions on Electrical and Electronic Materials
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    • v.7 no.6
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    • pp.313-318
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    • 2006
  • This paper introduces a partial discharge (PD) severity metric, S, based on the evaluation of time-sequence PD data capture and resulting Time-Sequence-Analysis Discharge (TSAD) level distributions. Basically based on an IEC60270 measurement technique, each PD event is time stamped and the discharge level noted. By evaluating the time differences between a previous and subsequent discharge, a 3D plot of time-sequence activity and discharge levels can be produced. From these parameters a measurement of severity, which takes into account dynamic or instantaneous variations in both the time of occurrence and the level of discharge, rather than using standard repetition rate techniques, can be formulated. The idea is to provide a measure of the severity of PD activity for potentially measuring the state of insulation within an item of plant. This severity measure is evaluated for a simple point-plane geometry in $SF_{6}$ as a function of gap distance and applied high voltage. The results show that as the partial discharge activity increases, the severity measure also increases. The importance of future investigations, quantifications and evaluations of the robustness, sensitivity and importance of such a severity measurement, as well as comparing it with typical repetition rate assessment techniques, and other monitoring techniques, are also very briefly discussed.

Difference of the Ginsenosides Contents According to the Planting Location in Panax ginseng C. A. Meyer (인삼의 재식 위치에 따른 진세노사이드 함량 차이)

  • Li, Xiangguo;Nam, Ki-Yeul;Choi, Jae-Eul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.2
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    • pp.159-164
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    • 2009
  • The difference of ginsenosides content according to placement of ginseng planting (line) under shading net in 5-year-old ginseng roots were examined. The total saponin ($Rb_1$, $Rb_2$, Rc, Rd, Re, and $Rg_1$) contents were 15.01 mg/g and 21.79 mg/g in the main roots, 35.93 mg/g and 43.32 mg/g in the lateral roots, 87.85 mg/g and 105.51 mg/g in the fine roots for the front $1st{\sim}2nd$ lines in Yunpoong and Landrace variety (purple-stem variant), respectively. In the middle $3rd{\sim}5th$ lines the total saponin contents were 18.73 mg/g and 23.19 mg/g in the main roots, 44.92 mg/g and 43.50 mg/g in the lateral roots, 92.97 mg/g and 110.70 mg/g in the fine roots in Yunpoong and Landrace variety, respectively. In the rear $6th{\sim}7th$ lines the total saponin contents were 21.88 mg/g and 26.68 mg/g in the main roots, 38.41 mg/g and 44.89 mg/g in the lateral roots, 101.03 mg/g and 107.06 mg/g in the fine roots in Yunpoong and Landrace variety, respectively. The differences in total and individual ginsenosides content in the main, lateral and fine roots among the lines were not significant but total ginsenosides contents in the main roots were different in case of Yunpoong variety. The ratios of protopanaxadiol (PD) type saponin to protopanaxatriol (PT) type saponin in roots were lower in the front lines compared to the middle and rear lines and the ratios were significantly different among the parts of roots.

Effects of in vivo-stresses on the Activities of the Myocardial Antioxidant Enzymes and the Ischemia-Reperfusion Injury in Rat Hearts (스트레스성 자극에 의한 항산화효소 유도와 허혈/재관류 심장 보호효과)

  • 박종완;김영훈;김명석
    • Toxicological Research
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    • v.11 no.1
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    • pp.161-168
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    • 1995
  • It has been found that various stress challenges induce the myocardial antioxidant enzymes and produce an acquisition of the cellular resistance to the ischemic injury in animal hearts. Most of the stresses, however, seem to be guite dangerous to an animal's life. In the present study, therefore, we tried to search for safely applicable stress modalities which could lead to the induction of antioxidant enzymes and the production of myocardial tolerance to the ischemia-reperfusion injury. Male Sprague-Dawley rats (200-250 g) were exposed to various non-fatal stress conditions, i.e., hyperthermia (environmental temperature of $42^{\circ}C$ for 30 min, non-anesthetized animal), iramobilization (60 min), treadmill exercise (20 m/min, 30min), swimming (30 min), and hyperbaric oxyflenation (3 atm, 60 min), once a day for 5 days. The activities of myocardial antioxidant enzymes and the ischemia-reperfusion injury of isolated hearts were evaluated at 24 hr after the last application of the stresses. The activities of antioxidant enzymes, superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase (G6PD), were assayed in the freshly excised ventricular tissues. The ischemia-reperfusion injury was produced by 20 min-global ischemia followed by 30 min-reperfusion using a Langendorff perfusion system. In swimming and hyperbaric oxygenation groups, the activities of SOD and G6PD increased significantly and in the hyperthermia group, the catalase activity was elevated by 63% compared to the control. The percentile recoveries of cardiac function at 30 min of the post-ischemic reperfusion were 55.4%, 73.4%, and 74.2% in swimming, the hyperbaric oxygenation and the hyperthermia groups, respectively. The values were significantly higher than that of the control (38.6%). In additions, left ventricular end-diastolic pressure and lactate dehydrogenase release were significantly reduced in the stress groups. The results suggest that the antioxidant enzymes in the heart could be induced by the apparently safe in vivo-stresses and this may be involved in the myocardial protection from the ischemia-reperfusion injury.

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Improvement of Liquid Culture Methods of Paecilomyces japonica (눈꽃동충하초균의 액체배양법 개선)

  • 남성희;정이연;조세연;한명세
    • Journal of Sericultural and Entomological Science
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    • v.43 no.1
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    • pp.33-36
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    • 2001
  • This study was carried out to improve the liquid culture methods of Paecilomyces japonica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150rpm under the photoperiod of 12L-12D. Dry weight of mycelium was averagely 1.216 g in the Sikworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6 g of 6 mmbeads in the SL medium, the dry weight is increased to 1.332 g. The optimal addition of silkworm larval powder to the culture medium for gest harvest was 1.360$\pm$0.67 g in dry weight.

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The Study of PM10, PM2.5 Mass Extinction Efficiency Characteristics Using LIDAR Data (라이다 데이터를 이용한 PM10, PM2.5 질량소산효율 특성 연구)

  • Kim, TaeGyeong;Joo, Sohee;Kim, Gahyeong;Noh, Youngmin
    • Korean Journal of Remote Sensing
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    • v.37 no.6_2
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    • pp.1793-1801
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    • 2021
  • From 2015 to June 2020, the backscattering coefficients of 532 and 1064 nm measured using LIDAR and the depolarization ratio at 532 nm were used to separate the backscattering coefficient at 532 nm as three types as PM10, PM2.5-10, PM2.5 according to particle size. The mass extinction efficiency (MEE) of three types was calculated using the mass concentration measured on the ground. The overall mean values of the calculated MEE were 5.1 ± 2.5, 1.7 ± 3.7, and 9.3 ± 6.3 m2/g in PM10, PM2.5-10, and PM2.5, respectively. When the mass concentration of PM10 and PM2.5 was low, higher than average MEE was calculated, and it was confirmed that the MEE decreased as the mass concentration increased. When the MEE was calculated for each type according to the mixing degree of Asian dust, PM2.5-10 was twice at pollution aerosol as high as 2.1 ± 2.8 m2/g, compare to pollution-dominated mixture, dust-dominated mixture, and pure dust of 1.1 ± 1.8, 1.4 ± 3.3, 1.1 ± 1.5 m2/g, respectively. However, PM2.5 MEE showed similar values irrespective of type: 9.4 ± 6.5, 9.0 ± 5.8, 10.3 ± 7.5, and 9.1 ± 9.0 m2/g. The MEE of PM10 was 5.6 ± 2.9, 4.4 ± 2.0, 3.6 ± 2.9, and 2.8 ± 2.4 m2/g in pollution aerosol (PA), pollution-dominated mixture (PDM), dust-dominated mixture (DDM), and pure dust (PD), respectively, and increased as the dust ratio value decreased. Even if the same type according to the same mass concentration or Asian dust mixture was shown, as the PM2.5/PM10 ratio decreased, the MEE of PM2.5-10 decreased and the MEE of PM2.5 showed a tendency to increase.

Effect of 6-Hydroxydopamine (6-OHDA) on the Expression of Testicular Steroidogenic Genes in Adult Rats

  • Heo, Hyun-Jin;Ahn, Ryun-Sup;Lee, Sung-Ho
    • Development and Reproduction
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    • v.14 no.3
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    • pp.199-205
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    • 2010
  • A neurotoxin, 6-hydroxydopamine (6-OHDA) has been widely used to create animal model for Parkinson's disease (PD). The present study was undertaken to examine whether depletion of brain dopamine (DA) stores with 6-OHDA can make alteration in the activities of the testicular steroidogenesis in adult rats. Young adult male rats (3 months old) were received a single dose of 6-OHDA (200 ${\mu}g$ in 10 ${\mu}{\ell}$/animal) by intracerebroventricular (icv) injection, and sacrificed after two weeks. The mRNA levels of steroidogenesis-related enzymes were measured by qRT-PCRs. Serum testosterone levels were measured by radioimmunoassay. Single icv infusion of 6-OHDA significantly decreased the mRNA levels of CYP11A1 (control:6-OHDA group=$1:0.68{\pm}0.14$ AU, p<0.05), CYP17 (control:6-OHDA group=$1:0.72{\pm}0.13$ AU, p<0.05). There were no changes in the mRNA levels of $3{\beta}$-HSD (control:6-OHDA group=$1:0.84{\pm}0.08$ AU) and $17{\beta}$-HSD (control: 6-OHDA group=$1:0.63{\pm}0.20$ AU), though the levels tended to be decreased in the 6-OHDA treated group. Administration of 6-OHDA decreased significantly the mRNA level of StAR when compared to the level of saline-injected control animals (control:6-OHDA group=$1:0.72{\pm}0.08$ AU, p<0.05). Treatment with single dose of 6-OHDA remarkably lowered serum testosterone levels compared to the levels of control group (control:6-OHDA group=$0.72{\pm}0.24:0.13{\pm}0.03ng/m{\ell}$, p<0.05). Taken together with our previous study, the present study demonstrated that the activities of hypothalamus-pituitary-testis hormonal axis could be negatively affected by blockade of brain DA biosynthesis, and suggested the reduced reproductive potential might be resulted in the animals. More precise information on the testicular steroidogenic activities in PD patients and PD-like animals should be required prior to the generalization of the sex steroid hormone therapy to meet the highest standards for safety and efficacy.